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AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX

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Chinese Journal of Polymer Science 2012年第5期30卷 705-718页

作者： Dong Li yang-ping wen 徐景坤贺浩华 Ming Liu Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of EducationKey Laboratory of PhysiologyEcology and Cultivation of Double Cropping RiceMinistry of AgricultureJiangxi Agricultural University

The biocompatiable and low-toxic poly（thiophene-3-aeetic acid）（PTAA） matrix was successfully electrosynthesized in ionic liquid 1-butyl-3-metbylimidazolium hexafluorophosphate （BmimPF6） in comparison with the electrosynthesis of PTAA matrix in acetonitrile （ACN）. Ascorbate oxidase （AO） was used as a model for the development and application of biosensor. Vitamin C （VC） biosensors were facilely fabricated by the covalent immobilization of AO molecules on PTAA matrices electrosynthesized in ACN containing tetrabutylammonium tetrafluoroborate and BmimPF6, respectively. Electrochemical impedance spectroscopy, scanning electron microscopy and FTIR spectroscopy indicated that AO molecules were covalently immobilized on PTAA matrices. Parameters of the as-obtained biosensors such as working potential, pH and temperature have been optimized. The amperometric biosensor based on PTAA matrix electrosynthesized in BmimPF6 exhibited wider linear range, lower detection limit, higher sensitivity and bioaffinity, and better operational and storage stability than that electrosynthesized in ACN under optimal conditions. The as-obtained biosensor based on PTAA matrix electrosynthesized in BmimPF6 was employed for the detection of VC content in comme：rcial juices, and the result was close to the data given by manufacturers. Excellent results indicate that the PTAA matrix electrosynthesized in ionic liquid is a promising platform for the covalent immobilization of biologically-active species and the development of biosensors.

关键词： Poly(thiophene-3-acetic acid) Ionic liquids Ascorbate oxidase Vitamin C Biosensor.

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POLY(THIOPHENE-3-ACETIC ACID)-PALLADIUM NANOPARTICLE COMPOSITE MODIFIED ELECTRODES FOR SUPERSENSITIVE DETERMINATION OF HYDRAZINE

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Chinese Journal of Polymer Science 2013年第3期31卷 419-426页

作者： Ou Zhang Hong-mei Yu Li-min Lu yang-ping wen 段学民徐景坤 School of Chemical Engineering University of Science and Technology Liaoning School of Pharmacy Jiangxi Science & Technology Normal University

A promising electrochemical sensor was fabricated by electrodeposition of Pd nanoparticles （PdNPs） on poly（thiophene-3-acetic acid）（PTAA）-modified glassy carbon electrode （GCE）, forming a PdNPs/PTAA composites-modified GCE （PdNPs/PTAA/GCE）. Scanning electron microscope （SEM） and electrochemical techniques were used for the characterization of these composites. It was found that the PdNPs/PTAA layer was very uniform. ]Electrochemical experiments showed that this proposed PdNPs/PTAA composites-modified electrode exhibited excellent electrocatalytic activity towards the oxidation of hydrazine. Under the optimum conditions, the proposed sensor can be applied to the quantification of hydrazine with a wide linear range from 8.0 × 10-9 mol/L to 1.0 × 10-5 mol/L with a low detection limit of 2.67 × 10-9 mol/L. The experiment results also showed that the sensor exhibited good reproducibility and long-term stability, as well as high selectivity with no interference from other potential competing species.

关键词： Sensor Hydrazine Nanocomposites Pd nanoparticles Conducting polymer.

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Electrochemical determination of quercetin by self-assembled platinum nanoparticles/poly(hydroxymethylated-3,4-ethylenedioxylthiophene) nanocomposite modified glassy carbon electrode

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Chinese Chemical Letters 2014年第4期25卷 505-510页

作者： Yuan-Yuan Yao Long Zhang Zi-Fei Wang Jing-Kun Xu yang-ping wen Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University College of Science Jiangxi Agricultural University

A simple and sensitive platinum nanoparticles/poly（hydroxymethylated-3,4-ethylenedioxylthiophene） nanocomposite （PtNPs/PEDOT-MeOH） modified glassy carbon electrode （GCE） was successfully developed for the electrochemical determination of quercetin. Scanning electron microscopy and energy dispersive X-ray spectroscopy results indicated that the PtNPs were inserted into the PEDOT- MeOH layer. Compared with the bare GCE and poly（3,4-ethylenedioxythiophene）（PEDOT） electrodes, the PtNPs/PEDOT-MeOH/GCE modified electrode exhibited a higher electrocatalytic ability toward the oxidation of quercetin due to the synergic effects of the electrocatalytic activity and strong adsorption ability of PtNPs together with the good water solubility and high conductivity of PEDOT-MeOH. The electrochemical sensor can be applied to the quantification of quercetin with a linear range covering 0.04-91μmol L-1 and a low detection limit of 5.2 nmol L-1. Furthermore, the modified electrode also exhibited good reoroducibilitv and long-term stability, as well as high selectivity.

关键词： Poly( 3 4-ethylenedioxylthiophene methanol) Platinum nanoparticles Electropolymerization Differential pulse voltammetryQuercetin

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POLY(3,4-ETHYLENEDIOXYTHIOPHENE METHANOL)/ASCORBATE OXIDASE/NAFION-SINGLE-WALLED CARBON NANOTUBES BIOSENSOR FOR VOLTAMMETRIC DETECTION OF VITAMIN C

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Chinese Journal of Polymer Science 2012年第3期30卷 460-469页

作者： yang-ping wen Dong Li Yao Lu 贺浩华徐景坤 Xue-min Duan Ming Liu Jiangxi Agr Univ Key Lab Crop Physiol Ecol&Genet BreedingMinist EducNanchang 330045Peoples R China Jiangxi Agr Univ Key Lab Physiol Ecol&Cultivat Double Cropping RMinist AgrNanchang 330045Peoples R China Jiangxi Sci&Technol Normal Univ Jiangxi Key Lab Organ ChemNanchang 330013Peoples R China Jiangxi Sci&Technol Normal Univ Sch PharmNanchang 330013Peoples R China

Vitamin C （VC） content in commercial juices was voltammetrically determined using a highly selective and sensitive poly（3,4-ethylenedioxythiophene methanol）/ascorbate oxidase/Nafion-single-walled carbon nanotubes （PEDOTM/AO/Nation-SWCNT） biosensor. The biocompatible PEDOTM matrix was prepared facilely by the one-step electrochemical deposition technique in lithium perehlorate aqueous solutions. AO was dip-coated on the surface of the biocompatible PEDOTM matrix. The mixture of Nafion-SWCNT was dip-cast onto the surface of AO layer when it was obtained by blending Nation solution and SWCNT dispersion together in a volume ratio of 1：1. The prepared PEDOTM/AO/Nafion-SWCNT biosensor was used for the voltammetric determination of VC, which exhibited the good linear range （4.0 ~ 10-s-3 x 10-s mol/L）, low detection limit （13 I^mol/L）, pronounced sensitivity （1.4072 mA （mmol/L） i cm 2）, high bioaftinity （low apparent Michaelis-Menten constant）, good stability （good repeatability）, high specificity （good anti-interference ability） coupled with the good reliability and feasibility （the determination of VC in commercial juices）. Meanwhile, the good aqueous solubility and the low onset oxidation potential of EDOTM will be more beneficial to the application in biosensor field compared to 3,4-ethylenedioxythiophene. Moreover, the good biocompatibility of PEDOTM matrix and high selectivity of Nation-SWCNT films also provide a promising platform for the development of biosensing devices.

关键词： Biosensor Vitamin C Poly(3,4-ethylenedioxythiophene methanol) Nation Single-walled carbon nanotubes.

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Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3,4-ethylenedioxythiophene) matrices for agricultural application in crops

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Chinese Chemical Letters 2012年第2期23卷 221-224页

作者： yang ping wen Li Min Lu Dong Li Ming Liu Hao Hua He Jing KunXu Key Lab of Crop Physiology Ecology and Genetic BreedingMinistry of EducationJiangxi Agricultural UniversityNanchang 330045China Jiangxi Key Lab of Organic Chemistry Jiangxi Science and Technology Normal UniversityNanchang 330013China

The vitamin C（VC） in crops was successfully determined using ascorbate oxidase（AO） electrochemical biosensor based on the biocompatible poly（3,4-ethylenedioxythiophene）（PEDOT） matrices,which was easily prepared by one-step electrodeposition technique in ionic liquid *** fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC,wide linear range,low detection limit,fast response time,good operational and storage stability,the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.

关键词： Biosensor Agricultural application Ionic liquid microemulsions Ascorbate oxidase Poly(3 4-ethyl-enedioxythiophene) Vitamin C

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Electrochemical sensor based on f-SWCNT and carboxylic group functionalized PEDOT for the sensitive determination of bisphenol A

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Chinese Chemical Letters 2014年第4期25卷 517-522页

作者： Long Zhang yang-ping wen Yuan-Yuan Yao Zi-Fei Wang Xue-Min Duan Jing-Kun Xu School of Pharmacy Jiangxi Science & Technology Normal University Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University

A simple, sensitive, and reliable method for the voltammetric determination of bisphenol A （BPA） by using carboxylic group functionalized single-walled carbon nanotubes （f-SWCNT）/carboxylic-functionalized poly（3,4-ethylenedioxythiophene）（PC4） complex modified glassy carbon electrode （GCE） has been successfully developed. The electrochemical behavior of BPA at the surface of the modified electrode is investigated by electrochemical techniques. The cyclic voltammetry results show that the as-prepared electrode exhibits strong catalytic activity toward the oxidation of BPA with a well-defined anodic peak at 0.623 V in PBS （0.1 mol/L, pH 7.0）. The surface morphology of the 3D network of composite film is beneficial for the adsorption ofanalytes. Under the optimized conditions, the oxidation peak current is proportional to BPA concentration in the range between 0.099 and 5.794 μmol/L （R2 = 0.9989）, with a limit of detection of 0.032 μmol/L （SIN = 3）. The enhanced performance of the sensor can be attributed to the excellent electrocatalytic property off-SWCNT and the extraordinary conductivity of PC4. Furthermore, the proposed modified electrode displays high stability and good reproducibility. The good result on the voltammetric determination of BPA also indicates that the asfabricated modified electrode will be a good candidate for the electrochemical determination and analysis of BPA.

关键词： Bisphenol A Electrochemical sensor Voltammetric determination Poly(3 4-ethylenedioxythiophene) derivative

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One-step co-electrodeposition of graphene oxide doped poly(hydroxymethylated-3,4-ethylenedioxythiophene) film and its electrochemical studies of indole-3-acetic acid

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Chinese Chemical Letters 2014年第4期25卷 511-516页

作者： Zi-Lan Feng Yuan-Yuan Yao Jing-Kun Xu Long Zhang Zi-Fei Wang yang-ping wen Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University College of Science Jiangxi Agricultural University

A novel graphene oxide （GO） doped poly（hydroxymethylated-3,4-ethylenedioxythiophene）（PEDOTM） film has been achieved via one-step co-electrodeposition and utilized for electrochemical studies of indole-3-acetic acid （IAA）. The incorporation of GO into PEDOTM film facilitated the electrocatalytic activity and exhibited a favorable interaction between the PEDOTM/GO film and the phytohormone during the oxidation of IAA. Under optimized conditions, differential pulse voltammetry and square wave voltammetry were used for the quantitative analysis of IAA, respectively, each exhibiting a wide linearity range from 0.6 μmol L-1 to 10 μmol L-1 and 0.05 μmol L-1 to 40 μmol L-1, good sensitivity with a low detection Iimit of 0.087 μmol L-1 and 0.033μmol L T, respectively, as well as good stability. With the notable advantages of a green, sensitive method, expeditious response and facile operation, the as-prepared PEDOTM/GO organic-inorganic composite film provides a promising platform for electrochemical studies of IAA.

关键词： Co-electrodeposition EDOT derivatives Graphene oxide Indole-3-acetic acid

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Zhongfeng Xingnao Liquid ameliorates post-stroke cognitive impairment through sirtuin1(SIRT1)/nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase 1(HO-1) pathway

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Chinese Journal of Natural Medicines 2025年第1期23卷 77-89页

作者： wenqin yang wen wen Hao Chen Haijun Zhang Yun Lu ping Wang Shijun Xu State Key Laboratory of Southwestern Chinese Medicine Resources Chengdu University of Traditional Chinese Medicine Institute of Material Medica Integration and Transformation for Brain Disorders Chengdu University of Traditional Chinese Medicine School of Pharmacy Chengdu University of Traditional Chinese Medicine Emergency Department Hospital of Chengdu University of Traditional Chinese Medicine

The activation of the sirtuin1(SIRT1)/nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase 1(HO-1) pathway has been shown to mitigate oxidative stress-induced apoptosis and mitochondrial damage by reducing reactive oxygen species(ROS) levels. Clinical trials have demonstrated that Zhongfeng Xingnao Liquid(ZFXN) ameliorates post-stroke cognitive impairment(PSCI). However, the underlying mechanism, particularly whether it involves protecting mitochondria and inhibiting apoptosis through the SIRT1/Nrf2/HO-1 pathway, remains unclear. This study employed an oxygen-glucose deprivation(OGD) cell model using SHSY5Y cells and induced PSCI in rats through modified bilateral carotid artery ligation(2VO).The effects of ZFXN on learning and memory, neuroprotective activity, mitochondrial function, oxidative stress, and the SIRT1/Nrf2/HO-1 pathway were evaluated both in vivo and in vitro. Results indicated that ZFXN significantly increased the B-cell lymphoma 2(Bcl2)/Bcl2-associated X(Bax) ratio, reduced terminal deoxynucleotidyl transferase-mediated d UTP nickend-labeling(TUNEL)+ cells, and markedly improved cognition, synaptic plasticity, and neuronal function in the hippocampus and cortex. Furthermore, ZFXN exhibited potent antioxidant activity, evidenced by decreased ROS and malondialdehyde(MDA) content and increased superoxide dismutase(SOD), catalase(CAT), and glutathione(GSH) levels. ZFXN also demonstrated considerable enhancement of mitochondrial membrane potential(MMP), Tom 20 fluorescence intensity, adenosine triphosphate(ATP) and energy charge(EC) levels, and mitochondrial complex Ⅰ and Ⅲ activity, thereby inhibiting mitochondrial damage. Additionally,ZFXN significantly increased SIRT1 activity and elevated SIRT1, nuclear Nrf2, and HO-1 ***, these effects were substantially counteracted when SIRT1 was suppressed by the inhibitor EX-527 in vitro. In conclusion, ZFXN alleviates PSCI by activating the SIRT1/Nrf2/HO-1 pathway and preventing mitochond

关键词： Apoptosis Mitochondrial function Oxidative stress Post-stroke cognitive impairment Sirtuin1/nuclear factor erythroid 2-related factor 2/heme oxygenase 1 pathway Zhongfeng Xingnao Liquid

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Evaluation of the shear stability of aerobic granular sludge from a pilot-scale membrane bioreactor:Establishment of a quantitative method

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Journal of Environmental Sciences 2025年第2期148卷 579-590页

作者： Biao yang Bingduo Wang Liying Bin Weirui Chen Xinyi Chen ping Li Shanglong wen Shaosong Huang Zhiqi Zhang Bing Tang School of Environmental Science and Engineering Guangdong University of TechnologyGuangzhou 510006China Guangdong Guangshen Environmental Protection Technology Co. Ltd.Guangzhou 510663China Guangdong Yuehai Water Investment Co. Ltd.Shenzhen 518000China

This work established a quantitative method to access the shear stability of aerobic granular sludge(AGS)and validated its feasibility by using the mature AGS from a pilot-scale(50 tons/day)membrane bioreactor(MBR)for treating real municipal *** results showed that the changing rate( S)of the peak area(S)of granule size distribution(GSD)exhibited an exponential relationship(R^(2)≥0.76)with the shear time(y=a−b·cx),which was a suitable indicative index to reflect the shear stability of different AGS *** limiting granule size(LGS)was defined and proposed to characterize the equilibrium size for AGS after being sheared for a period of time,whose value in terms of Dv50 showed high correlation(R^(2)=0.92)with the parameter *** free Ca^(2+)(28.44-34.21 mg/L)in the influent specifically interacted with polysaccharides(PS)in the granule’s extracellular polymeric substance(EPS)as a nucleation site,thereby inducing the formation of Ca precipitation to enhance its Young’s modulus,while Ca2+primarily interacted with PS in soluble metabolic product(SMP)during the initial granulation ***,the Young’s modulus significantly affected the parameter a related to shear stability(R^(2)=0.99).Since the parameter a was more closely related(R^(2)=1.00)to S than that of the parameter b or c,the excellent correlation(R^(2)=0.99)between the parameter a and the wet density further verified the feasibility of this method.

关键词： Shear stability Quantitative evaluation Aerobic granular sludge Membrane bioreactor Pilot-scale study Municipal wastewater

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Quantum metrology with optimal control under an arbitrary non-Markovian bosonic environment

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Communications in Theoretical Physics 2025年第2期77卷 112-120页

作者： Likun Zhou wen yang Beijing Computational Science Research Center

Finding the optimal control is of importance to quantum metrology under a noisy *** this paper, we tackle the problem of finding the optimal control to enhance the performance of quantum metrology under an arbitrary non-Markovian bosonic environment. By introducing an equivalent pseudomode model, the non-Markovian dynamic evolution is reduced to a Lindblad master equation, which helps us to calculate the gradient of quantum Fisher information and perform the gradient ascent algorithm to find the optimal control. Our approach is accurate and circumvents the need for the Born–Markovian approximation. As an example, we consider the frequency estimation of a spin with pure dephasing under two types of non-Markovian environments. By maximizing the quantum Fisher information at a fixed evolution time, we obtain the optimal multi-axis control, which results in a notable enhancement in quantum metrology. The advantage of our method lies in its applicability to the arbitrary non-Markovian bosonic environment.

关键词： quantum metrology non-Markovian environment optimal control

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