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Smad8 is involvement in follicular development via the regulation of granulosa cell growth and steroidogenesis in mice

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BIOCELL 2024年第1期48卷 139-147页

作者： DAOLUN YU DEYONG SHE KAI GE LEI yang ruina ZHAN SHAN LU YAFEI CAI College of Biotechnology and Pharmaceutical Engineering West Anhui UniversityLu’an237012China Lu’an Academy of Agricultural Sciences Lu’an237001China College of Life Sciences Anhui Normal UniversityWuhu241000China College of Animal Science and Technology Nanjing Agricultural UniversityNanjing210095China

Background:SMAD family proteins(SMADs)are crucial transcription factors downstream of transforming growth factor beta(TGF-ß)/SMAD signaling pathways that have been reported to play a pivotal role in mammalian ***,the role of SMAD family member 8(SMAD8,also known as SMAD9),a member of the SMAD family,in mammalian reproduction remains ***:We employed RNA interference techniques to knock down Smad8 expression in mouse granulosa cells(GCs)to investigate the effects of Smad8 on GC growth and ***:Our findings revealed a significant decrease in the proliferative capacity and a substantial increase in the apoptosis rate of GCs after transfection with Smad8-siRNA for 48 *** hormone assays demonstrated a significant decrease in estradiol(E2)levels,whereas progesterone(P4)remained *** mechanistic analysis showed that the mRNA expression of proliferating cell nuclear antigen(Pcna),Cyclin D2,cell cycle-dependent kinase 4(Cdk4),B-cell lymphoma-2(Bcl-2),estrogen receptor(Er),luteinizing hormone receptor(Lhr)and cytochrome P450 family 19 subfamily A member 1(Cyp19a1)significantly ***,the mRNA of cysteine aspartate proteinase 3(Caspase 3)significantly increased,wheras Bcl2-associated X(Bax),folliclestimulating hormone receptor(Fshr)and cytochrome P450 family 11 subfamily A member 1(Cyp11a1)remained unchanged compared to the ***:This study indicates that Smad8 knockdown inhibits cell proliferation,promotes apoptosis,reduces Er and Lhr transcription,and decreases E2 production in mouse *** findings suggest that Smad8 may serve as a novel genetic marker for mammalian reproduction.

关键词： Mouse Smad8 RNA interference Granulosa cells Growth and steroidogenesis

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Engineered plant extracellular vesicles for autoimmune diseases therapy

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Nano Research 2024年第4期17卷 2857-2873页

作者： ruina Han Yan Wu Yafei Han Xiangfei Liu Han Liu Jiacan Su Institute of Translational Medicine Shanghai UniversityShanghai 200444China Organoid Research Center Shanghai UniversityShanghai 200444China National Center for Translational Medicine(Shanghai)SHU Branch Shanghai UniversityShanghai 200444China Department of Orthopedics Shanghai Zhongye HospitalShanghai 200941China Department of Orthopedics Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China

Autoimmune diseases(AID)encompass a diverse array of conditions characterized by immune system dysregulation,resulting in aberrant responses of B cells and T cells against the body’s own healthy *** extracellular vesicles(PEVs)are nanoscale particles enclosed by phospholipid bilayers,secreted by plant cells,which facilitate intercellular communication by transporting various bioactive *** to their nanoscale structure,safety,abundant sources,low immunogenicity,high yield,biocompatibility,and effective targeting of the colon and liver,PEVs are regarded as a promising platform for the treatment of *** review provides a comprehensive summary of PEV biogenesis,physicochemical and biological properties,internalization mechanisms,isolation methods,and their applications in various diseases,with a specific focus on their potential roles in ***,we propose engineering approaches and administration methods for ***,we present an overview of the advantages and challenges associated with utilizing PEVs for the treatment of *** gaining a comprehensive understanding of PEVs,we anticipate the development of innovative therapeutic strategies for *** and engineered PEVs hold substantial promise as a valuable resource for innovative technologies in AID treatment.

关键词： autoimmune diseases plant extracellular vesicles extracellular vesicles engineering modification

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Improved Syntheses of Chrial BINAP with Stale Diphenylphosphine Oxide

Improved Syntheses of Chrial BINAP with Stale Diphenylphosph...

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河南省化学会2016年学术年会

作者： Hui Chen Yinlong Zhang Zhenqiang yang ruina yang Institute of Chemistry Henan Academy of Sciences

Chrial BINAP has drawn more attentions in past two decades because of its high catalytic activity in asymmetric catalysis. Although a number of methods of preparing BINAP and analogs have been reported, mostly air sensitive orgnophosphine compounds are involved. Here air stale diphenylphosphine oxide was used to synthesis BINAP. Chrial 2,2-dihydroxy-1,1-binaphthyl was firstly protected by trifluoromethanesulfonic anhydride, then after cross coupling with diphenylphosphine oxide and reduced by trichlorosilane, chrial BINAP was got in the yield of 82 % and 99 % ee.. Structure characterization of the intermediates was conducted by methods of PNMR、1HNMR and HRMS.

关键词： chrial BINAP syntheses diphenylphosphine oxide cross coupling reduce

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MicroRNAs in tumor stem cells

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The Chinese-German Journal of Clinical Oncology 2015年第2期14卷 92-96页

作者： Xiaochen Hu Junqiang yang Ruijie yang ruina yang Xinshuai Wang Shegan Gao Department of Oncology Cancer Institute the First Affiliated Hospital Cancer Institute Henan University of Science and Technology

MicroRNAs （miRNAs） are a class of non-coding RNAs that are believed to have a significant role in tumorigenesis and cancer metastasis. Cancer stem cells play a major role in tumor recurrence, metastasis, and drug resistance. Research has shown that miRNAs can promote or inhibit the sternness of cancer stem cells and regulate the differentiation and self-renewal of cancer stem cells. In this article, the phenotype and regulatory mechanisms of miRNAs in cancer stem cells will be described, together with an explanation of their potential role in tumor diagnosis and treatment.

关键词： miRNAs tumor stem cells sternness

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Study on the Synthesis and application of 4,6-bis(diphenylphosphino)phenoxazine

Study on the Synthesis and application of 4,6-bis(diphenylph...

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河南省化学会2016年学术年会

作者： Yinlong Zhang Hui Chen Shunwei Zhao ruina yang Institute of Chemistry Henan Academy of Sciences

4,6-bis(diphenylphosphino)phenoxazine was an important organic phosphorus ligand. It was widely used in hydroformylation, Palladium-catalyzed cross-coupling reaction, and C-H bond activation.4,6-bis(diphenylphosphino) phenoxazine was synthesized through protected by tertbutyldimethylsilyl chloride, introducing diphenylphosphine and off-protecting in 62% total yield. In the first step the solvent was THF, the temperature was 75 °C and n(phenoxazine):n( NaH):n( tertbutyldimethylsilyl chloride) = 1:1.25:1.5. In the second step the solvent was diethyl ether, and n(compound II):n( n-Bu Li):n( Ph2PCl):n( TMEDA) = 1:2.4:2.4:2.4. The protection group was removed with tetrabutylammonium fluoride in 24 h.

关键词： 4,6-bis(diphenylphosphino)phenoxazine cross-coupling organic phosphorus

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Synthesis and characterization of 7,7-dimethyl-7H-benzo[c]flourene

Synthesis and characterization of 7,7-dimethyl-7H-benzo[c]fl...

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河南省化学会2016年学术年会

作者： Kui Li Pengli Chen Zhenqiang yang Hui Chen ruina yang Institute of Chemistry Henan Academy of Sciences

Fluorene compounds used in photoelectric materials has researched and involved organic photoelectric molecular devices, electronic photographic materials, solar battery materials, color liquid crystal materials and cutting-edge technology fields such as organic optical information recording materials, has showed a broad application prospect. 7,7-dimethyl-7H-benzo[c]flourene is a very promising blu-ray material *** nitrogen protection, the Grignard reagent was synthesized used 1-bromonaphthalen as the starting material and tetrahydrofuran as solvent. 7,7-dimethyl-7H-benzo[c]flourene was synthesized with coupling reaction of 2-Bromochlorobenzene and The Grignard reagent preparation, with Addition reaction with acetone, with Closed loop reaction. The yield was 75%, and the content was 98%.1H NMR(400 MHz, DMSO-d6), δ: 8.799-8.778(d, 1H), 8.421-8.401(d, 1H), 8.051-8.031(d, 1H),7.961-7.940(d, 1H), 7.787-7.766(d, 1H), 7.716-7.552(m, 3H), 7.478-7.361(m, 2H), 1.501(s, 6H).

关键词： 1-bromonaphthalen 7,7-dimethyl-7H-benzo[c]flourene

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The synthesis and characterization of(7-bromo-9,9-dimethyl-9H-fluoren-2-yl)methanol

The synthesis and characterization of(7-bromo-9,9-dimethyl-9...

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河南省化学会2016年学术年会

作者： Chen Pengli Wang Chaojie Qu Fengbo yang Zhenqiang yang ruina Institute of Chemistry Henan Academy of Sciences

Organic electroluminescent materials are one of the hotspot in the field of photoelectric information functional materials. Fluorene derivatives have attracted considerable attention in recent years as organic light-emitting materials with development prospect and practical value of blue light emitting materials.(7-bromo-9,9-dimethyl-9H-fluoren-2-yl) methanol is a very promising blue-ray material *** nitrogen atmosphere, 7-bromo-9,9-dimethyl-9H-fluorene-2-carbaldehyde was synthesized with THF as solvent and 2,7-dibromo-9,9-dimethylfluorene as starting material at-78 °C. n-Bu Li was slowly added drop wise. After stirring for 1 hr, DMF was added at-78 °C. The reaction solution was poured into HCl and extracted with ethyl acetate. Recrystallization by Me OH as yellow solid. Yield: 52%。1HNMR(400 MHz, DMSO-d6), δ/ppm: 10.053(s,1H), 8.105-8.085(d, J=7.96 Hz, 2H), 7.956-7.914(m,3H), 7.622-7.597(m, 1H), 1.501(s, 6H).(7-bromo-9,9-dimethyl-9H-fluoren-2-yl)methanol was synthesized with reduction of7-bromo-9,9-dimethyl-9H-fluorene-2-carbaldehyde by Na BH4. Recrystallization by Me OH as white solid. Yield: 90%.1HNMR(400 MHz, DMSO-d6), δ/ppm:7.796-7.741(m, 3H), 7.523-7.486(m, 2H), 7.315-7.293(m,1H), 5.256-5.227(t, J=5.80 Hz 1H), 4.572-4.558(d, J=5.60 Hz, 2H), 1.438(s, 1H).

关键词： 2,7-dibromo-9,9-dimethylfluorene 7-bromo-9,9-dimethyl-9H-fluorene-2-carbaldehyde (7-bromo-9,9-dimethyl-9H-fluoren-2-yl) methanol

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The synthesis and characterization of 2,7-bis(4-bromophenyl)-9-hexylcarbazole

The synthesis and characterization of 2,7-bis(4-bromophenyl)...

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河南省化学会2016年学术年会

作者： Wang Chaojie Li Kui yang Zhenqiang Sun Minqing yang ruina Institute of Chemistry Henan Academy of Sciences

The derivatives of carbazole designed as blue-light chromospheres, have drawn much attention duo to their unique structures and excellent luminous properties in the electroluminescence. The bromine of2,7-bis(4-bromophenyl)-9-hexylcarbazole can carry out a variety of chemical modification. This method can improve the solubility of the product and the low temperature of the *** an argon atmosphere, 2,7-dibromo-9-hexylcarbazole(5.87 g), P-bromophenylboronic acid(6.53g) and tetrakistriphenylphosphine palladium(40 mg) were dissolved in toluene(20 ml). A 2M aqueous potassium phosphate solution(5ml) was added and the mixture was heated under reflux for 8 *** reaction mixture was poured into water and extracted with CH2Cl2. The organic layer was dried over Mg SO4, filtered and the solvent was removed by rotary evaporation. The product was isolated by recrystallization using toluene-Me OH mixed solvent. The product was a colorless solid(5.26 g). Purity97.5%, yield 65.4%. m.p.: 221.7-222.9 ℃. H NMR(400 MHz, CDCl3), δ: 0.841-0.876(3H, t),1.256-1.887(6H,m),1.869-1.943(2H,m),4.339-4.375(2H,t),7.409-7.433(2H,dd),7.520-7.522(2H,d),7.561-7.616(8H,m),8.113(1H,s),8.134(1H,s).

关键词： 2,7-dibromocarbazol 2,7-bis(4-bromophenyl)-9-hexyl-9H-carbazole

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数量柔性契约中的应急物资采购定价策略研究

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系统工程理论与实践 2016年第10期36卷 2590-2600页

作者：张琳田军杨瑞娜冯耕中西安交通大学管理学院西安710049

突发灾害情景下的应急物资需求面临爆发式增长的巨大不确定性,数量柔性契约可以为这类不确定性采购和供应提供保护性方案.本文构建了一个以政府主导的Stackelberg模型,通过常规采购和柔性采购相结合的方式,建立政企联合储备应急物资的... 详细信息

突发灾害情景下的应急物资需求面临爆发式增长的巨大不确定性,数量柔性契约可以为这类不确定性采购和供应提供保护性方案.本文构建了一个以政府主导的Stackelberg模型,通过常规采购和柔性采购相结合的方式,建立政企联合储备应急物资的合作关系.在推导获得政企双方的最优决策后,采用数值计算和敏感度分析方法,研究政府单位物资缺货成本和常规采购量等重要参数对政府采购成本和供应商收益的影响,获得以最大限度满足需求条件下的最优定价机制,以及相应的管理策略.结果显示,数量柔性契约可以有效地提高系统应急物资供应数量,并在实现政府成本控制的同时合理保护了供应商的收益.

关键词：应急物资储备单期采购数量柔性契约定价博弈帕累托改进

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豫西董家埝银矿床银的赋存状态研究

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矿床地质 2020年第3期39卷 486-500页

作者：杨睿娜纵瑞杨东潮河南省地质矿产勘查开发局第二地质矿产调查院河南郑州450001

董家埝银矿床位于豫西小秦岭地区小河岩体的南缘,矿体赋存于中元古代小河岩体与太古界太华群、中元古界官道口群、早古生界寒武系及新生界第四系地层的接触部位。文章通过矿相学研究、电子探针定量分析、能谱半定量分析及电子探针面扫... 详细信息

董家埝银矿床位于豫西小秦岭地区小河岩体的南缘,矿体赋存于中元古代小河岩体与太古界太华群、中元古界官道口群、早古生界寒武系及新生界第四系地层的接触部位。文章通过矿相学研究、电子探针定量分析、能谱半定量分析及电子探针面扫描分析等手段对该矿床矿石中银的赋存状态展开研究。结果表明,董家埝银矿床银矿物主要为自然银、辉银矿、硫锑铜银矿、金银矿、银金矿,载银矿物主要为黝铜矿、方铅矿、闪锌矿、黄铜矿,其中黝铜矿是银的主要载体矿物。银主要以可见银及不可见银赋存,可见银包括独立银矿物、显微包体银;不可见银包括晶格银、次显微包体银。赋存形式以独立银矿物、显微包体银及晶格银为主。

关键词：地球化学银的赋存状态矿石特征董家埝银矿床小秦岭

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