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Utilization of Ogura CMS germplasm with the clubroot resistance gene by fertility restoration and cytoplasm replacement in Brassica oleracea L

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Horticulture Research 2020年第1期7卷 1971-1980页

作者： Wenjing Ren Zhiyuan Li Fengqing Han Bin Zhang Xing Li Zhiyuan Fang limei yang Mu Zhuang Honghao Lv Yumei Liu Yong Wang Hailong Yu yangyong Zhang Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural CropsMinistry of Agriculture#12 Zhong Guan Cun Nandajie StreetBeijing 100081China

Clubroot disease,a major plant root disease caused by Plasmodiophora brassicae,has become one of the most destructive diseases among cultivated cruciferous ***,clubroot-resistant Brassica oleracea materials are rare.A few clubroot-resistant cabbage varieties are available on the market,but all are Ogura cytoplasmic male sterile(CMS)***,in this study,to reutilize the clubroot-resistant Ogura CMS germplasm of cabbage,a new fertility-restored Ogura CMS material,16Q2-11,was used as a bridge to transfer the clubroot resistance(CR)gene from the Ogura CMS cytoplasm to the normal cytoplasm by a two-step method(a fertility restoration and cytoplasm replacement method).In the first cross for fertility restoration of Ogura CMS clubroot-resistant cabbage(FRCRC),16Q2-11 was used as a restorer to cross with Ogura CMS materials containing the CR gene *** Rfo-positive progenies were generated,of which four contained CRb2:F8-514,F8-620,F8-732 and *** inoculation with race 4 of ***,these four CRb2-positive individuals showed ***,F8-514 and F8-839 were then used as male parents in the second cross of FRCRC to cross with cabbage inbred lines,resulting in the successful introgression of the CRb2 gene into the inbred *** offspring produced from this step of cross,which had a normal cytoplasm,showed a high resistance to race 4 of *** and could be utilized for the breeding of clubrootresistant cabbage varieties in the *** is the first time that the Ogura CMS restorer has been used to restore the fertility of Ogura CMS clubroot-resistant cabbages,which could improve germplasm diversity in cabbage and provide a reference method for using CMS germplasm in Brassica crops.

关键词： fertility germplasm breeding

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花似花,花非花——隐喻视角下的意象花卉

美术 2021年第1期 150-151页

作者：杨丽梅菏泽学院美术与设计学院

花与女人的关系非常密切,古人常把美丽柔弱的女人比喻成娇嫩的花。在中国文学的源头《诗经》里,花与女人之间就建立起了联系。如在《桃夭》篇中"桃之夭夭,灼灼其华",就以绽放的桃花比兴新娘。或许花朵鲜艳的色彩、动人的姿态都让人情不... 详细信息

花与女人的关系非常密切,古人常把美丽柔弱的女人比喻成娇嫩的花。在中国文学的源头《诗经》里,花与女人之间就建立起了联系。如在《桃夭》篇中"桃之夭夭,灼灼其华",就以绽放的桃花比兴新娘。或许花朵鲜艳的色彩、动人的姿态都让人情不自禁地想起女子的容貌和体态。花如女人,女子如花,花若与女人融为一体也就被称之为女人花。也许是因为生为女人,内向的性格,喜欢读古典诗词等个人特点,笔者从小就对花开花落之类安静而美好之事尤其向往。花卉也是十几年来笔者一直坚持的绘画题材,不过笔者对花卉的表达与传统做法不同,不去追求花卉的逼真写实.

关键词：绘画题材花开花落逼真写实古典诗词灼灼其华花卉桃之夭夭生为女人

Correlation Coefficients of MLST Technology in Streptococcus

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Animal Husbandry and Feed Science 2016年第4期8卷 207-210页

作者： Zhang Ying Ma Li Wang Jianjun Zhang Zhimei yang limei Shen Zhiqiang Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhou 256600 China Shandong Lvdu Ante Veterinary Drug Co. Ltd. Binzhou 256600 China

Multi-locus sequence typing（ MLST） was a newly defined genotyping method used for determining the sequences of nucleic acid. It was used to analyze the sequences of several housekeeping genes and helped to fix the final sequence types. The review was aimed to summarize the advantages and application of MLST in Streptococus agalactiae,S. dysgalactiae subspecies equisimilis,S. pneumonia,S. pyogenes and S. suis,including the amount of housekeeping genes,primer sequences and the size of PCR products.

关键词： Streptococcus subspecies Correlation primer allele recessive pathogenic mastitis typing summarize

已登记但未通知的应收账款转让效力分析与法律续造

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法学杂志 2022年第4期43卷 160-172页

作者：杨丽美中国社会科学院大学北京102401

区分原则是我国《民法典》确立的一项重要原则。在应收帐款转让中,合同订立行为与应收帐款转让行为之间在成立要件和法律效果上存在着清晰的区分。作为处分行为之一种的应收帐款转让,无论是出于保障交易安全还是提高交易效率的需要,应... 详细信息

区分原则是我国《民法典》确立的一项重要原则。在应收帐款转让中,合同订立行为与应收帐款转让行为之间在成立要件和法律效果上存在着清晰的区分。作为处分行为之一种的应收帐款转让,无论是出于保障交易安全还是提高交易效率的需要,应当进行公示。通知的主要效力是“知悉”,因此通知不能作为应收帐款转让对第三人生效的要件。应追溯“应收账款”及其转让行为的性质本源,借鉴《民法典》区分原则下的应收账款质押登记生效主义,尊重法典编纂体系,以法律续造的形式解决已登记但未通知的应收账款转让效力问题。

关键词：保理应收账款转让登记通知法律续造

Determination of Pulchinenoside B4 in Compound Radix Pulsatillae Granule

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Animal Husbandry and Feed Science 2016年第5期8卷 266-268,280页

作者： Zhang Ying yang limei Xu Qianqian Wang Yanping Guo Shijin Shen Zhiqiang Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhnu 256600 China Shandong Lvdu Ante Veterinary Drug Co. Ltd. Binzhou 256600 China

The determination method of pulchinenoside B4 in compound Radix Pulsatillae granule was established in the paper. The results laid a foundation for quality control of compound Radix Pulsatillae granule.

关键词： Pulchinenoside B4 Compound Radix Pulsatillae Granule Quality control

Pathogenicity of Escherichia coli O_(123) from Rex Rabbit on White Mice

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Animal Husbandry and Feed Science 2014年第4期6卷 216-217页

作者： Wang Yanping Guo Shijin yang limei Dong Lin Xu Qianqian Shen Zhiqiang Shandong Binzhou Animal Science and Veterinary Medicine Academy Shandong Lvduante Veterinary Drug Industry Co. Ltd.

[Objective]The paper was to analyze the pathogenesis of Escherichia coli O123 from rex rabbit. [Method]E. coli O123 isolated from rabbit liver with diarrhea symptom in scale rex rabbit farm was intraperitoneally injected into 18- 22 g Kunming mice,and its pathogenicity was determined by clinical symptoms and pathological examination. [Result]When the inoculation concentration was about 8. 5 × 107 CFU /mL,Kunming mice appeared the clinical symptoms of drooping spirit,diarrhea and gathering,and the mortality reached 50%. Anatomical examination found that intestinal wall was thinning and intestinal mucosa was bleeding. [Conclusion]E. coli from rex rabbit has strong pathogenicity,and establishing animal model with Kunming mice to study its pathogenesis is of great reference significance for diagnosis and prevention of E. coli disease of rex rabbit.

关键词： Scale rex rabbit farm E.coli from rex rabbit Pathogenicity

旋流电解提取铜冶炼污泥模拟浸出液中有价金属研究

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稀有金属 2015年第9期39卷 847-853页

作者：纪仲光王巍徐政杨丽梅北京有色金属研究总院生物冶金国家工程实验室北京100088

配制模拟铜冶炼污泥的硫酸浸出液作为电解原液,考察了旋流电解技术分离提取溶液中Cu,Zn,Ni 3种有价金属的效果。结果表明,旋流电解可以实现3种有价金属的分步电解分离,实验中直接获得了电解铜和电解锌产品。对于Cu,Zn,Ni混合溶液的旋流... 详细信息

配制模拟铜冶炼污泥的硫酸浸出液作为电解原液,考察了旋流电解技术分离提取溶液中Cu,Zn,Ni 3种有价金属的效果。结果表明,旋流电解可以实现3种有价金属的分步电解分离,实验中直接获得了电解铜和电解锌产品。对于Cu,Zn,Ni混合溶液的旋流电解过程,Cu优先在阴极电解,电解铜产品以铜粉为主,另含有少量的铜片;电流密度越大,Cu的沉积速度越快,在电流密度为0.04 A·cm-2时,30 min内Cu的回收率达到54.4%;由于Cu的电阻率较低,因此其在阴极上的沉积可以提高系统的导电性,从而使槽电压下降,降低能耗。对于Zn,Ni混合溶液的旋流电解过程,Zn优先于Ni电解,在阴极得到形貌较好的Zn板;槽电压随运行时间下降较电解铜时更加缓慢,30 min内仅下降9.1%;电解产品上部有Ni沉积,且表面由于氢的析出产生类似月球表面环形山的椭圆坑;旋流电解槽内部的旋流流态加之镍的特殊超电势使得Zn优先于Ni电解。

关键词：铜冶炼污泥模拟浸出液旋流电解超电势

肺癌患者静脉血栓栓塞预防的疗效与安全性的meta分析

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Journal of Chinese Pharmaceutical Sciences 2023年第10期32卷 852-860页

作者：苏俐玫毛溪悦阳丽梅福建医科大学基础医学院福建福州350000 福建医科大学省立临床医学院福建省立医院药学部福建福州350000

静脉血栓栓塞(VTE)是肺肿瘤患者的重要并发症和死亡风险之一。抗凝治疗在预防静脉血栓栓塞中起着重要作用,但其在肺癌患者中预防静脉血栓栓塞的安全性和有效性尚未得到系统评估。本研究旨在对肺癌患者预防性使用抗凝治疗的有效性和安全... 详细信息

静脉血栓栓塞(VTE)是肺肿瘤患者的重要并发症和死亡风险之一。抗凝治疗在预防静脉血栓栓塞中起着重要作用,但其在肺癌患者中预防静脉血栓栓塞的安全性和有效性尚未得到系统评估。本研究旨在对肺癌患者预防性使用抗凝治疗的有效性和安全性进行meta分析。对于2020年9月之前发表在Pubmed,Embase,中国知网(CNKI)和万方数据库上的文献进行系统性检索,根据纳入标准和排除标准对文献进行筛选,采用改良Jadad量表对纳入文献进行质量评价,利用Review Manager 5.4软件进行meta分析。对2272篇文献进行筛选,最后纳入4项研究,均为随机对照研究。质量评价结果显示有两篇为高质量文献(≥4分),其余文献得分均≤3分,属低质量文献研究。所有文献Jadad平均得分为3.5分。Meta分析结果显示,抗凝治疗可以显著降低肺癌患者VTE的发生率[RR=0.55, 95%CI (0.43, 0.70), P <0.00001],且患者总出血发生率[RR=1.74, 95%CI (0.87, 3.50), P=0.12],一般出血发生率[RR=1.64, 95%CI (0.66, 4.05), P=0.29],大出血发生率[RR=1.70,95%CI(0.91, 3.16),P=0.10]均无统计学差异。随机对照研究的meta分析结果显示,抗凝治疗可以有效降低肺癌患者的VTE发生率,患者治疗出血并发症发生率的差异无统计学意义,安全性较好。

关键词：肺癌静脉血栓栓塞抗凝治疗出血系统评价

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Influence of CYP2C9*3 and CYP4F2 rs2108622 gene polymorphisms on over-anticoagulation and bleeding complications of warfarin therapy in Chinese patients:a cohort study

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Journal of Chinese Pharmaceutical Sciences 2021年第6期30卷 484-494页

作者： Yushan Wu limei yang Xuhui Huang Feng Huang Tao Han Cardiac surgery Provincial Clinical College of Fujian Medical University/Fujian Provincial HospitalFuzhou 350001China Pharmacy Department Provincial Clinical College of Fujian Medical University/Fujian Provincial HospitalFuzhou350001China

In this retrospective cohort study,we aimed to identify the influence of the CYP2C9*3 and CYP4F2 rs2108622 gene alleles on over-anticoagulation and bleeding complications associated with warfarin therapy.A total of 196 patients were included,including 80 males,the mean age was 50.8±10.7 years,and the average follow-up was 26.9±11.8 *** patients underwent heart valve replacement surgery in the Cardiovascular Surgery of Fujian Provincial Hospital between January 2018 and August 2019,who took warfarin for at least 3 months and had target international normalized ratio(INR)between 1.8 and *** of CYP2C9*3 and CYP4F2 rs2108622 genes were tested by polymerase chain reaction(PCR)-gene sequencing ***19.0 software was utilized to analyze the association between genotypes and warfarin-related over-anticoagulation and bleeding *** the 434 patient-years,18 severe bleedings and 59 mild ones occurred in 31 *** with CYP2C9*1/*3 were associated with a higher over-anticoagulation risk compared with the*1/*1 carriers(hazard rate(HR)7.10;95%confidence interval(CI):2.54–19.79,P<0.001).The CYP4F2 rs2108622 mutant genotype did not cause significant increase in bleeding risk(HR 0.89;95%CI:0.43–1.82,P=0.74)or over-anticoagulation(HR 0.43;95%CI:0.16–1.13,P=0.09).Meanwhile,Kaplan-Meier survival curves showed that the time to over-anticoagulation in CYP2C9*1/*3 carriers was significantly shorter compared with the*1/*1 carriers(log-rank test,P<0.001),while that in CYP4F2 rs2108622 mutant genotype patients was longer compared with the wild-type patients(P=0.05).CYP2C9*3 and CYP4F2 rs2108622 might be major predictive factors of over-anticoagulation for warfarin therapy in Chinese patients.

关键词： Anticoagulation Genetics Bleeding disorders&coagulopathies Risk management