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The separation and recovery of copper(Ⅱ), nickel(Ⅱ), cobalt(Ⅱ), zinc(Ⅱ),and cadmium(Ⅱ) in a sulfate-based solution using a mixture of Versatic 10 acid and Mextral 984H

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Chinese Journal of Chemical Engineering 2017年第6期25卷 760-767页

作者： Yinxuan Qiu limei yang Songtao Huang Zhongguang Ji Yan Li National Engineering Laboratory of Biohydrometallurgy General Research Institute for Non-Ferrous Metals Beijing 100088 China

We studied the separation and recovery of copper(Ⅱ), nickel(Ⅱ), cobalt(Ⅱ), zinc(Ⅱ), and cadmium(Ⅱ) from magnesium and calcium, using synergistic solvent extraction(SSX) in a typical hydrometallurgical waste solution. A mixture of Versatic 10 acid and Mextral 984 H, diluted with Mextral DT100, was used to obtain fundamental data on p H and distribution isotherms, as well as the kinetics of extraction and stripping. We also investigated the main effects and interactions of common solvent extraction factors: the extraction p H at equilibrium, the temperature, and the extractant concentration. The synergistic effect for extracting metals was confirmed. The results showed that the addition of Mextral 984 H enhanced the separation factors of copper, nickel, cobalt,zinc, and cadmium over magnesium and calcium. Compared with Versatic 10 acid alone, for a mixture of0.5 mol·L^(-1) Versatic 10 acid/0.5 mol·L^(-1)Mextral 984 H, Δp H50 values of copper, nickel, cobalt, zinc, and cadmium were found to be N 2.0, 3.30, 2.85, 0.95, and 1.32 p H units, respectively. The Δp H_(50)(Zn–Mg)and Δp H_(50)(Zn–Ca)values were 3.27 and 2.25, respectively, indicating easy separation and recovery of copper, nickel, zinc, cobalt,and cadmium. The extraction and stripping of copper, cobalt, zinc, and cadmium were fast, with 90% of the metal transferred in 2 min. We next studied whether the metals could be stripped from the extracted liquid selectively in sequence, by using sulfuric acid at different concentrations. The influence of the molecular structure of the oxime and carboxylic acid components upon the synergistic effects was identified by numerical *** separation of copper, nickel, cobalt, and zinc over magnesium and calcium was achieved with this synergistic solvent extraction system.

关键词： Water treatment Solvent extraction Versatic 10 acid Mextral 984HRecovery

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An update on the arsenal:mining resistance genes for disease management of Brassica crops in the genomic era

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Horticulture Research 2020年第1期7卷 2357-2374页

作者： Honghao Lv Zhiyuan Fang limei yang yangyong Zhang Yong Wang Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural CropsMinistry of Agriculture12#Zhongguancun South StreetBeijing 100081China

Brassica species include many economically important crops that provide nutrition and health-promoting substances to humans ***,as with all crops,their production is constantly threatened by emerging viral,bacterial,and fungal diseases,whose incidence has increased in recent *** methods of control are often costly,present limited effectiveness,and cause environmental damage;instead,the ideal approach is to mine and utilize the resistance genes of the Brassica crop hosts ***,the development of genomics,molecular genetics,and biological techniques enables us to rapidly discover and apply resistance(R)***,the R genes identified in Brassica crops are summarized,including their mapping and cloning,possible molecular mechanisms,and application in resistance *** perspectives concerning how to accurately discover additional R gene resources and efficiently utilize these genes in the genomic era are also discussed.

关键词： crops resistance utilize

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Enhanced Cooling Efficiency of Urban Trees on Hotter Summer Days in 70 Cities of China

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Advances in Atmospheric Sciences 2024年第11期41卷 2259-2275页

作者： limei yang Jun GE Yipeng CAO Yu LIU Xing LUO Shiyao WANG Weidong GUO School of Atmospheric Sciences Nanjing UniversityNanjing 210023China Joint International Research Laboratory of Atmospheric and Earth System Sciences Nanjing UniversityNanjing 210023China Frontiers Science Center for Critical Earth Material Cycling Nanjing UniversityNanjing 210023China

Increasing the urban tree cover percentage(TCP) is widely recognized as an efficient way to mitigate the urban heat island effect. The cooling efficiency of urban trees can be either enhanced or attenuated on hotter days, depending on the physiological response of urban trees to rising ambient temperature. However, the response of urban trees' cooling efficiency to rising urban temperature remains poorly quantified for China's cities. In this study, we quantify the response of urban trees' cooling efficiency to rising urban temperature at noontime [~1330 LT(local time), LT=UTC+8] in 17summers(June, July, and August) from 2003–19 in 70 economically developed cities of China based on satellite observations. The results show that urban trees have stronger cooling efficiency with increasing temperature, suggesting additional cooling benefits provided by urban trees on hotter days. The enhanced cooling efficiency values of urban trees range from 0.002 to 0.055℃ %-1 per 1℃ increase in temperature across the selected cities, with larger values for the lowTCP-level cities. The response is also regulated by background temperature and precipitation, as the additional cooling benefit tends to be larger in warmer and wetter cities at the same TCP level. The positive response of urban trees' cooling efficiency to rising urban temperature is explained mainly by the stronger evapotranspiration of urban trees on hotter *** results have important implications for alleviating urban heat risk by utilizing urban trees, particularly considering that extreme hot days are becoming more frequent in cities under global warming.

关键词： urban trees cooling efficiency China's cities evapotranspiration summer hot days

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Recent advances in catalyst-modified Mg-based hydrogen storage materials

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Journal of Materials Science & Technology 2023年第32期163卷 182-211页

作者： Yaxiong yang Xin Zhang Lingchao Zhang Wenxuan Zhang Huifeng Liu Zhenguo Huang limei yang Changdong Gu Wenping Sun Mingxia Gao Yongfeng Liu Hongge Pan Institute of Science and Technology for New Energy Xi’an Technological UniversityXi’an 710021China State Key Laboratory of Silicon Materials Key Laboratory of Advanced Materials and Applications for Batteries of Zhejiang Province and School of Materials Science and EngineeringZhejiang UniversityHangzhou 310058China School of Civil&Environmental Engineering University of Technology Sydney81 BroadwayUltimoNSW2007Australia

The storage of hydrogen in a compact,safe and cost-effective manner can be one of the key enabling technologies to power a more sustainable *** hydride(MgH_(2))has attracted strong research interest as a hydrogen carrier because of its high gravimetric and volumetric hydrogen ***,the practical use of MgH_(2)for hydrogen storage has been limited due to high operation temperatures and sluggish *** is of crucial importance for the enhancement of hydrogen cycling kinetics of Mg/MgH_(2)and considerable work has been focused on designing,fabricating and optimizing *** review covers the recent advances in catalyzed Mg-based hydrogen storage *** fundamental properties and the syntheses of MgH_(2)as a hydrogen carrier are first briefly *** that,the general catalysis mechanisms and the catalysts developed for hydrogen storage in MgH_(2)are summarized in ***,the challenges and future research focus are *** studies indicate that transition metals,rare-earth metals and their compounds are quite effective in catalyzing hydrogen storage in Mg/MgH_(2).Most metal-containing compounds were converted in situ to elemental metal or their magnesium alloys,and their particle sizes and dispersion affect their catalytic *** in-situ construction of catalyzed ultrasmall Mg/MgH_(2)nanostructures(<10 nm in size)is believed to be the future research *** important insights will help with the design and development of high-performance catalysts for hydrogen storage in Mg/MgH_(2).

关键词： Hydrogen storage Magnesium hydride Kinetics Catalysts Reversibility

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Genome-wide identification and analysis of cytokinin dehydrogenase/oxidase(CKX) family genes in Brassica oleracea *** their involvement in response to Plasmodiophora brassicae infections

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Horticultural Plant Journal 2022年第1期8卷 68-80页

作者： Mingzhao Zhu Yong Wang Shujin Lu limei yang Mu Zhuang yangyong Zhang Honghao Lv Zhiyuan Fang Xilin Hou State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of AgricultureInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China

Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple ***,a comprehensive analysis of cytokinin dehydrogenase/oxidase(CKX)family genes in cabbage has not been *** this study,a total of 36 CKX genes were identified using a genome-wide search *** analysis classified these genes into three *** were distributed unevenly across nine chromosomes in ***,and 15 of them did not contain any *** results of colinearity analysis showed that 36 CKX gene in Arabidopsis was present in several copies in the Brassica oleracea *** analysis of cisacting elements indicated that all genes possessed at least one stress or hormone responsive cis-acting element.A heatmap of CKX gene expression showed the patterns of expression of these genes in various tissues and *** genes(Bol028363,Bol031036 and Bol018140)were relatively highly expressed in all of the investigated tissues under normal conditions,showing the expression profile of housekeeping ***,the expression patterns of CKX genes in Jingfeng 1 and Xiangan 336 were quite different under the same ***,three genes(Bol020547,Bol028392 and Bol045724)were significantly down-regulated and up-regulated in the susceptible and resistant material,respectively,after inoculation,which may indicate their crucial roles in resistance to clubroot *** results provide insights for better understanding the roles of CKX genes in the ***–*** interaction.

关键词： Brassica oleracea Genome-wide Cytokinin dehydrogenase/oxidase(CKX)genes Clubroot

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A pair of transporters controls mitochondrial Zn^(2+) levels to maintain mitochondrial homeostasis

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Protein & Cell 2022年第3期13卷 180-202页

作者： Tengfei Ma Liyuan Zhao Jie Zhang Ruofeng Tang Xin Wang Nan Liu Qian Zhang Fengyang Wang Meijiao Li Qian Shan yang yang Qiuyuan Yin limei yang Qiwen Gan Chonglin yang State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan and Center for Life Sciences School of Life SciencesYunnan UniversityKunming 650091China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Zn^(2+)is required for the activity of many mitochondrial proteins,which regulate mitochondrial dynamics,apoptosis and ***,it is not understood how the proper mitochondrial Zn^(2+)level is achieved to maintain mitochondrial *** Caenorhabditis elegans,we reveal here that a pair of mitochondrion-localized transporters controls the mitochondrial level of Zn^(2+).We demonstrate that SLC-30A9/ZnT9 is a mitochondrial Zn^(2+)*** of SLC-30A9 leads to mitochondrial Zn^(2+)accumulation,which damages mitochondria,impairs animal development and shortens the life *** further identify SLC-25A25/SCaMC-2 as an important regulator of mitochondrial Zn^(2+)*** of SLC-25A25 suppresses the abnormal mitochondrial Zn^(2+)accumulation and defective mitochondrial structure and functions caused by loss of ***,we reveal that the endoplasmic reticulum contains the Zn^(2+)pool from which mitochondrial Zn^(2+)is *** findings establish the molecular basis for controlling the correct mitochondrial levels for normal mitochondrial structure and functions.

关键词： mitochondria Zn^(2+)transporter C.elegans ER-mitochondrial contact development

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Map-based cloning and CRISPR/Cas9-based editing uncover BoNA1 as the causal gene for the no-anthocyanin-accumulation phenotype in curly kale (Brassica oleracea var. sabellica)

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Horticulture Research 2023年第8期10卷 194-202页

作者： Kaiwen Yuan Xinyu Zhao Wenru Sun limei yang yangyong Zhang Yong Wang Jialei Ji Fengqing Han Zhiyuan Fang Honghao Lv State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing100081China

Brassica oleracea comprises several important vegetable and ornamental crops,including curly kale,ornamental kale,cabbage,broccoli,and *** accumulation of anthocyanins,important secondary metabolites valuable to human health,in these plants varies widely and is responsible for their pink to dark purple *** curly kale varieties lack anthocyanins,making these plants completely *** genetic basis of this trait is still *** crossed the curly kale inbred line BK2019(without anthocyanins)with the cabbage inbred line YL1(with anthocyanins)and the Chinese kale inbred line TO1000(with anthocyanins)to generate segregating *** no-anthocyanin trait was genetically controlled by a recessive gene,*** generated a linkage map and mapped bona1 to a 256-kb interval on *** identified one candidate gene,Bo9g058630,in the target genomic region;this gene is homologous to AT5G42800,which encodes a dihydroflavonol-4-reductase-like(DFR-like)protein in *** BK2019,a 1-bp insertion was observed in the second exon of Bo9g058630 and directly produced a stop *** verify the candidate gene function,CRISPR/Cas9 gene editing technology was applied to knock out *** generated three bona1 mutants,two of which were completely green with no anthocyanins,confirming that Bo9g058630 corresponds to *** insertion/deletion mutations in BoNA1 exons were found in all six of the other no-anthocyanin kale varieties examined,supporting that independent disruption of BoNA1 resulted in no-anthocyanin varieties of *** study improves the understanding of the regulation mechanism of anthocyanin accumulation in *** subspecies.

关键词： crops fir insertion

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Dissection of two QTL clusters underlying yield-related heterosis in the cabbage founder parent 01–20

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Horticultural Plant Journal 2023年第1期9卷 77-88页

作者： Xing Li Honghao Lv Bin Zhang Zhiyuan Fang limei yang Mu Zhuang Yumei Liu Zhansheng Li Yong Wang yangyong Zhang Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesMinistry of Agriculture and Rural AffairsBeijingChina

Cabbage has significant heterosis and most commercial cultivars are *** explore genetic basis of cabbage heterosis and promote cabbage heterosis utilization,we constructed two populations by crossing 100 DH lines derived from a cabbage hybrid 01–20×96–100 with two female *** exhibited different extents of heterosis,the mean value of economic yield was 2.6 times bigger than *** identified 66 and 73 QTLs associated with mid-parent heterosis and transgressive heterosis of twelve yield-related traits,*** QTLs could be detected under the two-year experiment existed in two populations with different testers,showing relatively high phenotypic contribution rate(15.8%–20.0%).Heterosis QTLs exhibited clustered distribution in several cabbage chromosome *** dominant genetic regions,mk300–316 and mk258–268,originated from the elite parent 01–20,exhibited significant genetic effects for yield-related heterosis,which were first *** elite DH lines(D22,D46,D83)harboring these two dominant regions were selected as having strong heterosis in cabbage *** gene analysis revealed that some genes participating in biosynthetic processes of carbohydrates and some responses to auxin might affect cabbage yield *** identification and genetic dissection of yield-related traits provide new insights into the genetic effects of cabbage heterosis.

关键词： Cabbage Yield-related traits Heterosis QTL Genetic analysis New inbred line breeding

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Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers

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Horticultural Plant Journal 2021年第5期7卷 423-433页

作者： Jingjing Huang Yumei Liu Fengqing Han Zhiyuan Fang limei yang Mu Zhuang yangyong Zhang Honghao Lv Yong Wang Jialei Ji Zhansheng Li Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of AgricultureBeijing 100081China

To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)*** pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and *** 10 pairs of SNP primers were selected,producing 78 *** diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to *** observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 *** clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and *** study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese *** K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed *** Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple *** simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Q<0.6,18(11.18%)broccoli cultivars belonged to the mixed *** study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials *** meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar id

关键词： Broccoli Genetic diversity Population structure analysis Fingerprint SNP

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Heterotic Group Classification of 63 Inbred Lines and Hybrid Purity Identification by Using SSR Markers in Winter Cabbage(Brassica Oleracea L. var. capitata)

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Horticultural Plant Journal 2018年第4期4卷 158-164页

作者： LI Xing YU Hailong LI Zhiyuan LIU Xiaoping FANG Zhiyuan LIU Yumei yang limei ZHUANG Mu LV Honghao ZHANG yangyong Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences

Winter cabbage is an important crop cultivated through winter in the region near the yangtze River, enabling the supply of fresh cabbage there at that time of year. However, a problem has emerged regarding the newly developed parents of winter cabbage, which is completely different from spring and autumn cabbage, namely, how to combine these parents to breed an elite hybrid. To classify the heterotic groups and improve the efficiency of parent selection in winter cabbage breeding, 20 polymorphic SSR markers were selected to screen 63 winter cabbage inbred lines. Seventeen pairs among the 20 SSR markers amplified polymorphic bands. These primers amplified two to six bands,with an average of 2.8 bands per primer, and a total of 47 polymorphic bands were generated in the 63 inbred lines. These lines included flatheaded morphotype and round-headed morphotype, thus they were separately classified into heterotic groups based on the SSR markers. The flat-headed morphotype contained 21 inbred lines and was classified into three heterotic groups, named Hanchun 4, Jiali, and Dongsheng, in accordance with the representative germplasm contained in each group. The round-headed morphotype contained 42 inbred lines and was classified into five heterotic groups, named Parte, Bejo1039, YK-143, SCA002, and Golden B90. Meanwhile, parent analysis of 20 developed elite combinations showed that their parents were all distributed in different heterotic groups, indicating that the group classification was reasonable,which can provide a basis for further parent selection in winter cabbage breeding. Furthermore, polymorphic SSR primers were successfully used to identify the hybrid purity of three elite varieties.

关键词： winter cabbage head morphotype SSR marker heterotic group hybrid purity identification

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