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Adult adipose-derived stromal cells differentiate into neurons with normal electrophysiological functions

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Neural Regeneration Research 2011年第34期6卷 2681-2686页

作者： Xiaodong Yuan yanan Cai Ya ou Yanhui Lu Department of Neurology Kailuan General Hospital Hebei Union University Tangshan 063000 Hebei Province China

β-mercaptoethanol was used to induce in vitro neuronal differentiation of adipose-derived stromal cells. Within an 8-hour period post-differentiation, the induced cells exhibited typical neuronal morphology, and expression of microtubule-associated protein 2 and neuron-specific enolase, which are markers of mature neurons, reached a peak at 5 hours. Specific organelle Nissl bodies of neurons were observed under transmission electron microscopy. Results of membrane potential showed that fluorescence intensity of cells was greater after 5 hours than adipose-derived stromal cells prior to induction. In addition, following stimulation with high-concentration potassium solution, fluorescence intensity increased. These experimental findings suggested that neurons differentiated from adipose-derived stromal cells and expressed mature K^＋ channels. In addition, following stimulation with high potassium solution, the membrane potential depolarized and fired an action potential, confirming that the induced cells possessed electrophysiological functions.

关键词： adipose-derived stromal cells differentiation membrane potential neurons ultrastructure electrophysiological functions DiBAC4 (3)

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《ESH高血压管理指南2023》解读

护理研究 2024年第15期38卷 2633-2640页

作者：欧敏行薛亚男张秀杰大连医科大学附属第一医院辽宁116000

从护理视角对《欧洲高血压学会(ESH)高血压管理指南2023》概述、评估、预防及随访等方面内容进行解读,旨在为相关护理声明或指南的制定与更新提供依据,不断规范与完善高血压病人的专科护理,为临床护理实践提供循证指导。

关键词：高血压管理指南护理

《2023 AHA/ACC/ACCP/ASPC/NLA/PCNA慢性冠状动脉疾病患者管理指南》要点解读

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中国全科医学 2024年第18期27卷 2173-2178,2191页

作者：薛亚男欧敏行张秀杰孟庆雪刘莹大连医科大学附属第一医院心力衰竭与高血压科辽宁省大连市116011 大连医科大学辽宁省大连市116044 大连医科大学附属第一医院心力衰竭与结构性心脏病科辽宁省大连市116011

慢性冠状动脉疾病是指因心脏流入或流出血流不足所引起的慢性心脏或血管疾病,发病率与死亡率居高不下,导致巨大的个人、经济与社会负担。2023年7月美国心脏协会(AHA)、美国心脏病学会(ACC)等6个学术组织联合发布了《2023 AHA/ACC/ACCP/A... 详细信息

慢性冠状动脉疾病是指因心脏流入或流出血流不足所引起的慢性心脏或血管疾病,发病率与死亡率居高不下,导致巨大的个人、经济与社会负担。2023年7月美国心脏协会(AHA)、美国心脏病学会(ACC)等6个学术组织联合发布了《2023 AHA/ACC/ACCP/ASPC/NLA/PCNA慢性冠状动脉疾病患者管理指南》,该指南提供了一种以循证为基础和以患者为中心的方法综合管理慢性冠状动脉疾病患者,内容涉及慢性冠状动脉疾病的流行病学,以及对患者的评估、诊断与风险分层、治疗、特殊人群管理,患者随访和相关注意事项等建议,强调了健康饮食、规律体育锻炼和远离烟草对心血管健康的重要性,并且更新了钠-葡萄糖协同转运蛋白2抑制剂和胰高血糖素样肽-1受体激动剂等药物的相关治疗推荐,为临床工作提供了最新的基于循证医学证据的建议。本文对该指南的管理策略进行要点解读,旨在为国内相关指南的制订与更新提供依据,不断规范与完善慢性冠状动脉疾病患者的专科诊疗照护,以期为临床实践提供循证指导,改善慢性冠状动脉疾病患者的预后。

关键词：冠心病慢性冠状动脉疾病心血管疾病管理指南解读美国心脏协会

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脂肪模拟物及其在食品中的应用研究进展

食品科学 2024年第15期45卷 351-359页

作者：沈雪丽蒲媛媛曹亚楠赵江林邹亮欧逢莲景凯妮杨婷宋雨成都大学食品与生物工程学院四川成都610106 农业农村部杂粮加工重点实验室四川成都610106 四川省杂粮产业化工程技术研究中心四川成都610106 广元市妇幼保健院四川广元628040 云南师范大学生命科学学院云南昆明650500

脂肪是食品重要的营养成分之一,不仅能赋予食物独特的风味和口感,提供人体所需要的能量,还会对食品的结构和整体品质产生重要影响。但是过量摄入脂肪不利于人体健康,因此希望通过使用脂肪模拟物代替脂肪,在尽可能保持食品原有风味的情... 详细信息

脂肪是食品重要的营养成分之一,不仅能赋予食物独特的风味和口感,提供人体所需要的能量,还会对食品的结构和整体品质产生重要影响。但是过量摄入脂肪不利于人体健康,因此希望通过使用脂肪模拟物代替脂肪,在尽可能保持食品原有风味的情况下降低过量摄入脂肪对人体的危害。本文对脂肪替代品和脂肪模拟物的分类及近年来脂肪模拟物在食品中的作用及应用研究进行综述,概述了脂肪替代品分为脂肪替代物和脂肪模拟物的分类依据,重点阐述了脂肪模拟物中单一型脂肪模拟物和复合型脂肪模拟物替代脂肪的原理、优点、局限性及其在食品中的作用及应用,并指出未来研发策略,以期为开发健康安全的脂肪模拟物提供思路。

关键词：脂肪脂肪模拟物分类应用

民办职业教育现状及经费投入体制机制创新研究

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职业技术 2024年第3期23卷 67-73页

作者：欧咏梅许诺崔亚楠邱志兴解福泉广州华夏职业学院广州510935

民办职业教育是我国教育体系的重要组成部分,为我国经济社会的发展培养了数千万技能型人才,在中国式现代化建设中发挥着人才培育功能。但我国民办职业教育的发展走深、走实、走远遇到了制约瓶颈。文章以民办职业教育与公办高职教育的经... 详细信息

民办职业教育是我国教育体系的重要组成部分,为我国经济社会的发展培养了数千万技能型人才,在中国式现代化建设中发挥着人才培育功能。但我国民办职业教育的发展走深、走实、走远遇到了制约瓶颈。文章以民办职业教育与公办高职教育的经费投入比较为切入点,描述了民办职业教育的困境及当前教育经费分配现状,为给民办职业教育创造良好的投入机制,建议采取补贴或资助的方式对民办职业教育给予资助,建立民办职业教育奖补机制,以保障民办职业教育的教育教学水平。

关键词：民办职业教育经费投入机制创新

ACE2-Targeting antibody suppresses SARS-CoY-2 Omicron and Delta variants

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Signal Transduction and Targeted Therapy 2022年第3期7卷 613-615页

作者： Jianxia ou yanan Zhang Yongmei Wang Zherui Zhang Hongping Wei Junping Yu Qi Wang Guifeng Wang Bo Zhang Chunhe Wang Biotheropeutics Discovery Research Center Shanghai Institute of Materia MedicoShanghaiChina School of Chinese Materia Medica Nanjing University of Chinese MedicineNanjingChina Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhanChina University of Chinese Academy of Sciences BeijingChina

Dear Editor,The pandemic of COVID-19 continues worldwide with many variants arising,especially SARS-CoV-2 Delta(B.1.617.2)and Omicron(B.1.1.529)variants of concern(VOCs).According to WHO,Omicron has spread in almost a... 详细信息

关键词： Delta doubling variants

Apoptosis during β-mercaptoethanol-induced differentiation of adult adipose-derived stromal cells into neurons

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Neural Regeneration Research 2011年第10期6卷 750-755页

作者： yanan Cai Xiaodong Yuan Ya ou Yanhui Lu Department of Neurology Kailuan General Hospital Hebei United University Tangshan 063000 Hebei Province China

β-mercaptoethanol can induce adipose-derived stromal cells to rapidly and efficiently differentiate into neurons in ***,because of the short survival time of the differentiated cells,clinical applications for this technique are *** such,we examined apoptosis of neurons differentiated from adipose-derived stromal cells induced with β-mercaptoethanol in vitro using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and transmission electron *** results revealed that the number of surviving cells decreased and apoptosis rate increased as induction time *** together,these results suggest that apoptosis occurring in the process of adipose-derived stromal cells differentiating into neurons is the main cause of cell ***,the mechanism underlying cellular apoptosis should be researched further to develop methods of controlling apoptosis for clinical applications.

关键词： adult adipose-derived stromal cells induced differentiation neurons ultrastructure apoptosis 13-mercaptoethanol neural regeneration

A novel ethanol-based method to induce differentiation of adipose-derived stromal cells into astrocytes

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Neural Regeneration Research 2011年第10期6卷 738-743页

作者： Ya ou Xiaodong Yuan yanan Cai Yanhui Lu Department of Neurology Kailuan General Hospital Hebei United University Tangshan 063000 Hebei Province China

The quantity and survival time of astrocytes,which were differentiated from adult adipose-derived stromal cells after exposure to an inducer containing 3-isobutyl-1-methylxanthine,have thus far been *** present study investigated the growth and differentiation characteristics of induced astrocytes by observing their growth *** induction for 48 hours with an inducer containing 0.5% ethanol,some adult adult adipose-derived stromal cells displayed typical astrocytic *** cell quantity gradually decreased with prolonged induction ***,glial fibrillary acidic protein,and S-100 expression reached peak levels at 14 days,but neuron-specific enolase was not *** results suggest that the induced astrocytes reached their peak at 14 *** optimization of the culture environment may yield mature astrocytes with normal functions,in greater quantity,and prolonged survival time.

关键词： adult adipose-derived stromal cells induced differentiation astrocyte morphology growth characteristics neural regeneration

Viral receptor profiles of masked palm civet revealed by single-cell transcriptomics

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Journal of Genetics and Genomics 2022年第11期49卷 1072-1075页

作者： Peiwen Ding Haoyu Wang Jiacheng Zhu Fuyu An Jinqian Xu Xiangning Ding Lihua Luo Weiying Wu Qiuyu Qin yanan Wei Wandong Zhao Zhiyuan Lv Haimeng Li Yixin Zhu Meiling Li Wensheng Zhang yanan Zhang Zhihua ou Huan Liu Yan Hua College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China BGI-Shenzhen ShenzhenGuangdong 518083China Guangdong Provincial Key Laboratory of Silviculture Protection and UtilizationGuangdong Academy of ForestryGuangzhouGuangdong 510520China Department of Neurobiology NHC and CAMS Key Laboratory of Medical NeurobiologySchool of Brain Science and Brian Medicineand the MOE Frontier Science Center for Brain Research and Brain–Machine IntegrationZhejiang University School of MedicineHangzhouZhejiang 310012China School of Basic Medicine Qingdao UniversityQingdaoShandong 266071China School of Basic Medical Sciences Binzhou Medical UniversityYantaiShandong 264003China Tsinghua-Berkeley Shenzhen Institute Tsinghua UniversityShenzhenGuangdong 518000China Shenzhen Key Laboratory of Unknown Pathogen Identification BGI-ShenzhenShenzhenGuangdong 518083China

Civets are small mammals belonging to the family *** masked palm civets(Paguma larvata)served as an intermediate host in the bat-to-human transmission of severe acute respiratory syndrome coronavirus(SARS-Co V)in 2003(Guan et al.,2003).Because of their unique role in the SARS outbreak,civets were suspected as a potential intermediate host of SARS-Co V-2.

关键词： Vive mask intermediate