检索结果-南通市图书馆

Genetic factors contributing to human primary ciliary dyskinesia and male infertility

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2017年第5期19卷 515-520页

作者： Zhi-Yong Ji yan-Wei Sha Lu Ding ping Li The Center for Reproductive Medicine Xiamen Maternity and Child Care Hospital No. 10 Zhenhai Road Xiamen China

Primary ciliary dyskinesia （PCD） is an autosomal-recessive disorder resulting from the loss of normal ciliary function. Symptoms include neonatal respiratory distress, chronic sinusitis, bronchiectasis, situs inversus, and infertility. However, only 15 PCD-associated genes have been identified to cause male infertility to date. Owing to the genetic heterogeneity of PCD, comprehensive molecular genetic testing is not considered the standard of care. Here, we provide an update of the progress on the identification of genetic factors related to PCD associated with male infertility, summarizing the underlying molecular mechanisms, and discuss the clinical implications of these findings. Further research in this field will impact the diagnostic strategy for male infertility, enabling clinicians to provide patients with informed genetic counseling, and help to adopt the best course of treatment for developing directly targeted personalized medicine.

关键词： genetic factors Kartagener syndrome male infertility primary ciliary dyskinesia

Differential protein expression during colonic adaptation in ultra-short bowel rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第20期17卷 2572-2579页

作者： Hai-ping Jiang Tao Chen Guang-Rong yan Dan Chen Department of Gen-eral Surgery the First Affiliated Hospital of Jinan UniversityGuangzhou 510630 Guangdong Province China Institute of Life and Health Engineering and National Engineering and Research Center for Genetic Medicine Jinan University Guangzhou 510632 Guangdong Province China

AIM: To investigate the proteins involved in colonic adaptation and molecular mechanisms of colonic adaptation in rats with ultra-short bowel syndrome (USBS). METHODS: Sprague Dawley rats were randomly assigned to three groups: USBS group (10 rats) undergoing an approximately 90%-95% small bowel resection; sham-operation group (10 rats) undergoing small bowel transaction and anastomosis; and control group (ten normal rats). Colon morphology and differential protein expression was analyzed after rats were given postsurgical enteral nutrition for 21 d. Protein expression in the colonic mucosa was analyzed by two-dimensional electrophoresis (2-DE) in all groups. Differential protein spots were detected by ImageMaster 2D Platinum soft-ware and were further analyzed with matrix-assisted laser desorption/ionization-time-of-flight/time-of-flightmass spectrometric (MALDI-TOF/TOF-MS) analysis. RESULTS: The colonic mucosal thickness significantly increased in the USBS group compared with the control group (302.1 ± 16.9 μm vs 273.7 ± 16.0 μm, P 0.05). The height of colon plica markedly improved in USBS group compared with the control group (998.4 ± 81.2 μm vs 883.4 ± 39.0 μm, P 0.05). A total of 141 differential protein spots were found in the USBS group. Forty-nine of these spots were down-regulated while 92 protein spots were up-regulated by over 2-folds. There were 133 differential protein spots in USBS group. Thirty of these spots were down-regulated and 103 were upregulated. There were 47 common differential protein spots among the three groups, including 17 downregulated protein spots and 30 up-regulated spots. Among 47 differential spots, eight up-regulated proteins were identified by MALDITOF/TOF-MS. These proteins were previously reported to be involved in sugar and fat metabolism, protein synth

关键词：蛋白表达 SD大鼠结肠差分超短基质辅助激光解吸蛋白质表达蛋白质点

Discovery of a highly potent and orally available importin-β1 inhibitor that overcomes enzalutamide-resistance in advanced prostate cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2023年第12期13卷 4934-4944页

作者： Jia-Luo Huang Xue-Long yan Dong Huang Lu Gan Huahua Gao Run-zhu Fan Shen Li Fang-Yu Yuan Xinying zhu Gui-Hua Tang Hong-Wu Chen Junjian Wang Sheng Yin School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China School of Pharmacy Guizhou Medical UniversityGuian New DistrictGuizhou 550025China School of Medicine University of California DavisSacramentoCA 95817USA

Nuclear transporter importin-β1 is emerging as an attractive target by virtue of its prevalence in many cancers.However,the lack of druggable inhibitors restricts its therapeutic proof of concept.In the present work,we optimized a natural importin-β1 inhibitor DD1 to afford an improved analog DD1-Br with better tolerability(>25 folds)and oral bioavailability.DD1-Br inhibited the survival of castration-resistant prostate cancer(CRPC)cells with sub-nanomolar potency and completely prevented tumor growth in resistant CRPC models both in monotherapy(0.5 mg/kg)and in enzalutamidecombination therapy.Mechanistic study revealed that by targeting importin-β1,DD1-Br markedly inhibited the nuclear accumulation of multiple CRPC drivers,particularly AR-V7,a main contributor to enzalutamide resistance,leading to the integral suppression of downstream oncogenic signaling.This study provides a promising lead for CRPC and demonstrates the potential of overcoming drug resistance in advanced CRPC via targeting importin-β1.

关键词： Importin-β1 CRPC Drug discovery Enzalutamide-resistance Natural product Cancer Daphnane diterpenoid Nuclear transporter

Molecular Dynamics Simulation of Temperature-dependent Flexibility of Thermophilic Xylose Isomerase

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Physics 2009年第5期22卷 467-472,I0001页

作者： Wei Xu ping Cai Ming yan Lin Xu ping-kai Ouyang School of Chemical and Biological Engineering Yancheng Institute of Technology Yancheng 224051 China State Key Laboratory of Materials-Oriented Chemical Engineering College of Life Science and Pharmacy Nanjing University of Technology Nanjing 220009 China

有 D 木糖的 Thermus thermophilus 木糖 isomerase (TtXI ) 的复杂模型被构造，并且分子的动力学(MD ) 模拟被 NAMD2.5 为 10 ns 在 300 和 360 K 执行。旋转(Rg ) 的半径，子单元相互作用，和残余灵活性被分析。结果表演残余 6069， 1... 详细信息

有 D 木糖的 Thermus thermophilus 木糖 isomerase (TtXI ) 的复杂模型被构造，并且分子的动力学(MD ) 模拟被 NAMD2.5 为 10 ns 在 300 和 360 K 执行。旋转(Rg ) 的半径，子单元相互作用，和残余灵活性被分析。结果表演残余 6069， 142148， 169172，和 332340 在 300 和 360 K 有高灵活性。有在 360 K 的更高的灵活性的残余能主要在 300 K 比那被划分成二个组：一个人在由残余组成的 helix-loop-helix 区域定位 5580 在里面催化领域；其它在子单元连接。在 360 K 的催化领域的 Rg 显示出 0.16 吗？高，比那，在小 C 终端的 300 K，而是 Rg，领域没有明显的差别。结果显示催化领域的那提高的 Rg 可以导致 TtXI 的活跃地点的强烈运动并且支持 D 木糖 isomization 反应。八张氢契约和五离子对与 300 K 相比在 360 K 在子单元接口被减少，那可以是为在在在 TtXI 的高温度的活动的刚硬和增加的减少的主要原因。也，解释 TtXI E372G 异种的冷改编的现象的帮助以前报导了的这结果。我们的结果揭示在温度和 TtXI 的结构灵活性之间的关系，并且在与多重子单元理解 thermophile 蛋白质的 thermostability 起一个重要作用。

关键词：木糖异构酶分子动力学结构柔性

Overexpression of MiR-633 Suppresses the Tumorigenicity of Gastric Cancer Cells and Induces Apoptosis by Targeting MAPK1

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current Medical Science 2022年第5期42卷 1033-1045页

作者： Hai-long LI Yao-hui SONG Zheng-ping DU Yong-hua HU zhuan-xiong WANG Xi CHEN Xing-mei LU Ying-xia CHEN Yong-qiang DUAN Xiang-dong zhu Department of Geriatrics Affiliated Hospital of Gansu University of Traditional Chinese MedicineLanzhou730000China Department of Internal Medicine The First Clinical Medical CollegeGansu University of Chinese MedicineLanzhou730000China Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and The Prevention and Treatment with Traditional Chinese Medicine in Gansu Colleges and Universities Gansu University of Chinese MedicineLanzhou730000China School of Basic Medicine Gansu Vocational College of HealthLanzhou730300China College of Traditional Chinese Medicine Ningxia Medical UniversityYinchuan750004China

Objective MicroRNA(miRNA/miR)-633 is dysregulated in several types of cancers and is involved in tumorigenesis.However,the function and role of this miRNA in gastric cancer(GC)are not fully understood.The aim of the present study was to evaluate miR-633 expression in GC cell lines and in GC tissue vs.adjacent normal tissue,and to determine its association with clinicopathological data.This work was extended to investigate the effects of miR-633 overexpression on tumor cells in vitro.Methods Reverse transcription-quantitative PCR(RT-qPCR)was used to detect and compare the expression level of miR-633 in GC cells,as well as in GC and normal adjacent tissue samples.The clinical significance of miR-633 was also analyzed.MiR-633 lentivirus(LV-miR-633)and negative control lentivirus(LV-NC)were generated and used to transduce SGC-7901 and HGC-27 GC cells in order to analyze the effect of miR-633 on their phenotype.The effects of miR-633 overexpression on GC cell proliferation,apoptosis,migration and invasion were investigated.The target gene of miR-633 was predicted,then confirmed using a dual luciferase reporter gene assay,RT-qPCR and Western blotting.Results MiR-633 was significantly downregulated in GC cell lines,as well as in GC tissue compared with adjacent normal tissue.Moreover,miR-633 expression was associated with the tumor/node/metastasis(TNM)stage,invasion depth,Borrmann classification and lymph node metastasis(P<0.05).Compared with the LV-NC group,transduction with LV-miR-633 reduced the proliferation,the number of clones,the wound healing rate,the number of invading cells and the number of cells in the G1 phase of the cell cycle(P<0.01).LV-miR-633 also increased the apoptosis rate(P<0.01).The expression level of mitogen-activated protein kinase(MAPK)1,high-mobility group box 3(HMGB3),claudin 1(CLDN1)and MAPK13 were downregulated in LV-miR-633-transduced cells(P<0.01).The dual luciferase reporter assay confirmed that the 3′-untranslated region of MAPK1 was the target site of miR-633(P<0.01).Conclusion MiR-633

关键词： miR-633 gastric cancer apoptosis metastasis mitogen-activated protein kinase 1

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

粗糙度对硅烷环氧杂化树脂涂层附着力影响

环境技术 2024年第2期42卷 49-57页

作者：慕仙莲何卫平张雪原刘元海朱利敏中国特种飞行器研究所结构腐蚀防护与控制航空科技重点实验室荆门448035

目的研究基体粗糙度对涂层附着力的影响。方法选取LY12铝合金、硅烷环氧杂化树脂涂层为研究对象,开展不同粗糙度下涂层与基体间附着力的影响关系研究,确定粗糙度对涂层机械附着力的微观作用机理,并通过有限元软件ABAQUS6.12建立硅烷环... 详细信息

目的研究基体粗糙度对涂层附着力的影响。方法选取LY12铝合金、硅烷环氧杂化树脂涂层为研究对象,开展不同粗糙度下涂层与基体间附着力的影响关系研究,确定粗糙度对涂层机械附着力的微观作用机理,并通过有限元软件ABAQUS6.12建立硅烷环氧杂化树脂涂层/铝合金基体的界面损伤粘结模型,模拟涂层/基体界面从起始剥离到完全开裂的过程,并通过对比分析不同基体粗糙度与涂层S22应力值的关系,从而验证基体表面粗糙度对涂层附着力的作用机理为机械互锁理论。结果随着基体表面粗糙度的增加,涂层的附着强度逐渐增大,但二者并不呈线性关系,当硅烷环氧杂化树脂涂层的厚度为(30±2)um时,铝合金基体表面粗糙度Ra=(3.6~4.8)um范围内,可保证涂层较好的附着力。结论凹凸不平的基体表面可增大涂层/基体间的接触面积,可以使涂层与基体像铆钉一样牢固结合,从而提高涂层与基体的吸附作用力。

关键词：粗糙度涂层附着力有限元粘结模型应力值机械互锁理论

In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第9期12卷 3682-3693页

作者： yanqiu Gu Rong Wang Panpan Chen Shengnan Li Xinyi Chai Chun Chen Yue Liu yan Cao Diya Lv Zhanying Hong Zhenyu zhu Yifeng Chai Yongfang Yuan Xiaofei Chen Department of Pharmacy Shanghai Ninth People's HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 201999China School of Pharmacy Naval Medical University(Second Military Medical University)Shanghai 200433China Shanghai Key Laboratory for Pharmaceutical Metabolite Research Shanghai 200433China

Cell membrane affinity chromatography has been widely applied in membrane protein(MP)-targeted drug screening and interaction analysis.However,in current methods,the MP sources are derived from cell lines or recombinant protein expression,which are time-consuming for cell culture or purification,and also difficult to ensure the purity and consistent orientation of MPs in the chromatographic stationary phase.In this study,a novel in situ synthesis membrane protein affinity chromatography(iSMAC)method was developed utilizing cell-free protein expression(CFE)and covalent immobilized affinity chromatography,which achieved efficient in situ synthesis and unidirectional insertion of MPs into liposomes in the stationary phase.The advantages of iSMAC are:1)There is no need to culture cells or prepare recombinant proteins;2)Specific and purified MPs with stable and controllable content can be obtained within 2 h;3)MPs maintain the transmembrane structure and a consistent orientation in the chromatographic stationary phase;4)The flexible and personalized construction of cDNAs makes it possible to analyze drug binding sites.iSMAC was successfully applied to screen PDGFRβinhibitors from Salvia miltiorrhiza and Schisandra chinensis.Micro columns prepared by in-situ synthesis maintain satisfactory analysis activity within 72 h.Two new PDGFRβinhibitors,salvianolic acid B and gomisin D,were screened out with KD values of 13.44 and 7.39μmol/L,respectively.In vitro experiments confirmed that the two compounds decreasedα-SMA and collagen Ⅰ mRNA levels raised by TGF-βin HSC-T6 cells through regulating the phosphorylation of p38,AKT and ERK.In vivo,Sal B could also attenuate CCl_(4)-induced liver fibrosis by downregulating PDGFRβdownstream related protein levels.The iSMAC method can be applied to other general MPs,and provides a practical approach for the rapid preparation of MP-immobilized or other biological solid-phase materials.

关键词： Affinity chromatography In situ synthesis of membrane proteins Unidirectional insertion PDGFRβ Drug screening Ligand–protein interaction Antihepatic fibrosis

Molecular evidence on the presence of Schistosoma japonicum infection in snails along the yangtze River, 2015–2019

在线全文

学校读者我要写书评

暂无评论

Infectious Diseases of Poverty 2022年第3期11卷 54-62页

作者： Yin-Long Li Hui Dang Su-Ying Guo Li-Juan Zhang Yun Feng Song-Jun Ding Xiao-Wei Shan Guang-ping Li Min Yuan Jing Xu Shi-zhu Li National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)Shanghai200025People’s Republic of China NHC Key Laboratory of Parasite and Vector Biology Shanghai200025People’s Republic of China WHO Collaborating Centre for Tropical Diseases Shanghai200025People’s Republic of China National Center for International Research on Tropical Diseases Shanghai200025People’s Republic of China Jiangsu Provincial Institute of Schistosomiasis Control WuxiJiangsu Province214064People’s Republic of China Anhui Provincial Institute of Schistosomiasis Control HefeiAnhui Province230061People’s Republic of China Hubei Provincial Institute of Schistosomiasis Control Hubei Center for Disease ControlWuhanHubei Province430079People’s Republic of China Hunan Provincial Institute of Schistosomiasis Control Hunan Province 414000YueyangPeople’s Republic of China Jiangxi Provincial Institute of Parasitic Disease NanchangJiangxi Province330006People’s Republic of China

Background: Due to sustained control activities,the prevalence of Schistosoma japonicum infection in humans,livestock and snails has decreased significantly in P.R.China,and the target has shifted from control to elimination according to the Outline of Healthy China 2030 Plan.Applying highly sensitive methods to explore the presence of S.japonicum infection in its intermediate host will benefit to assess the endemicity or verify the transmission interruption of schistosomiasis accurately.The aim of this study was to access the presence of S.japonicum infection by a loop-mediated isothermal amplification(LAMP)method through a 5-year longitudinal study in five lake provinces along the yangtze River.Methods: Based on previous epidemiological data,about 260 villages with potential transmission risk of schistosomiasis were selected from endemic counties in five lake provinces along the yangtze River annually from 2015 to 2019.Snail surveys were conducted in selected villages by systematic sampling method and/or environmental sampling method each year.All live snails collected from field were detected by microscopic dissection method,and then about one third of them were detected by LAMP method to assess the presence of S.japonicum infection with a single blind manner.The infection rate and nucleic acid positive rate of schistosomes in snails,as well as the indicators reflecting the snails’distribution were calculated and analyzed.Fisher's exact test was used to examine any change of positive rate of schistosomes in snails over time.Results: The 5-year survey covered 94,241 ha of environment with 33,897 ha of snail habitats detected accumulatively.Totally 145.3 ha new snail habitats and 524.4 ha re-emergent snail habitats were found during 2015–2019.The percentage of frames with snails decreased from 5.93%[45,152/761,492,95%confidence intervals(CI):5.88–5.98%]in 2015 to 5.25%(30,947/589,583,95%CI:5.19–5.31%)in 2019,while the mean density of living snails fluctuated but presented a downward trend generally from 0.20 sna

关键词： Schistosomajaponicum Dissection Loop-mediated isothermal amplification The yangtze River Transmission risk Low endemic area

An efficient bifunctional Ni-Nb_(2)O_(5)nanocatalysts for the hydrodeoxygenation of anisole

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2022年第9期35卷 187-197页

作者： Juan Xu ping zhu Islam HEl Azab Ben Bin Xu Zhanhu Guo Ashraf YElnaggar Gaber AMMersal Xiangyi Liu Yunfei Zhi Zhiping Lin Hassan Algadi Shaoyun Shan Faculty of Chemical Engineering Kunming University of Science and TechnologyKunming 650500China College of Chemical Engineering Southwest Forestry UniversityKunming 650224China Yunnan Province Special Equipment Safety Inspection and Research Institute Kunming 650228China Department of Food Science and Nutrition College of ScienceTaif UniversityTaif 21944Saudi Arabia Department of Mechanical and Construction Engineering Faculty of Engineering and EnvironmentNorthumbria UniversityNewcastle upon Tyne NE18STUK Integrated Composites Lab(ICL) Department of Chemical&Biomolecular EngineeringUniversity of TennesseeKnoxvilleTN 37996USA Department of Chemistry College of ScienceTaif UniversityTaif 21944Saudi Arabia Taizhou University Taizhou 31800China Department of Electrical Engineering Faculty of EngineeringNajran UniversityNajran 11001Saudi Arabia

The Ni-Nb_(2)O_(5)nanocatalysts have been prepared by the solgel method,and the catalytic hydrodeoxygenation(HDO)performance of anisole as model compound is studied.The results show that Nb exists as amorphous Nb_(2)O_(5)species,which can promote Ni dispersion.The addition of Nb_(2)O_(5)increases the acidity of the catalyst.However,when the content of niobium is high,there is an inactive Nb-Ni-O mixed phase.The size and morphology of Ni grains in catalysts are different due to the difference of Nb/Ni molar ratio.The Ni_(0.9)Nb_(0.1)sample has the largest surface area of 170.8 m^(2)·g^(-1)among the catalysts prepared in different Nb/Ni molar ratios,which is mainly composed of spherical nanoparticles and crack pores.The HDO of anisole follows the reaction route of the hydrogenation HYD route.The Ni_(0.9)Nb_(0.1)catalyst displayed a higher HDO performance for anisole than Ni catalyst.The selectivity to cyclohexane over the Ni_(0.9)Nb_(0.1)sample is about 10 times that of Ni catalyst at 220℃and 3 MPa H_(2).The selectivity of cyclohexane is increased with the increase of reaction temperature.The anisole is almost completely transformed into cyclohexane at 240℃,3 MPa H_(2)and 4 h.

关键词： Hydrodeoxygenation(HDO) Catalysts Ni-Nb_(2)O_(5) Solgel method Anisole