Overexpression of MiR-633 Suppresses the Tumorigenicity of Gastric Cancer Cells and Induces Apoptosis by Targeting MAPK1
作者机构：Department of GeriatricsAffiliated Hospital of Gansu University of Traditional Chinese MedicineLanzhou730000China Department of Internal MedicineThe First Clinical Medical CollegeGansu University of Chinese MedicineLanzhou730000China Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and The Prevention and Treatment with Traditional Chinese Medicine in Gansu Colleges and UniversitiesGansu University of Chinese MedicineLanzhou730000China School of Basic MedicineGansu Vocational College of HealthLanzhou730300China College of Traditional Chinese MedicineNingxia Medical UniversityYinchuan750004China
出 版 物：《Current Medical Science》 (当代医学科学（英文）)
年 卷 期：2022年第42卷第5期
页 面：1033-1045页
核心收录：
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学]
基 金：supported by grants from Natural Science Foundation of Gansu Province(No.20JR5RA189 and No.17JR5RA169) the Foundation of the fundamental scientific research funds for colleges and universities in Gansu Province[No.(2014)63-15] the National Natural Science Fundation of China(No.81760830)
主 题：miR-633 gastric cancer apoptosis metastasis mitogen-activated protein kinase 1
摘 要：Objective MicroRNA(miRNA/miR)-633 is dysregulated in several types of cancers and is involved in ***,the function and role of this miRNA in gastric cancer(GC)are not fully *** aim of the present study was to evaluate miR-633 expression in GC cell lines and in GC tissue *** normal tissue,and to determine its association with clinicopathological *** work was extended to investigate the effects of miR-633 overexpression on tumor cells in *** Reverse transcription-quantitative PCR(RT-qPCR)was used to detect and compare the expression level of miR-633 in GC cells,as well as in GC and normal adjacent tissue *** clinical significance of miR-633 was also ***-633 lentivirus(LV-miR-633)and negative control lentivirus(LV-NC)were generated and used to transduce SGC-7901 and HGC-27 GC cells in order to analyze the effect of miR-633 on their *** effects of miR-633 overexpression on GC cell proliferation,apoptosis,migration and invasion were *** target gene of miR-633 was predicted,then confirmed using a dual luciferase reporter gene assay,RT-qPCR and Western *** MiR-633 was significantly downregulated in GC cell lines,as well as in GC tissue compared with adjacent normal ***,miR-633 expression was associated with the tumor/node/metastasis(TNM)stage,invasion depth,Borrmann classification and lymph node metastasis(P0.05).Compared with the LV-NC group,transduction with LV-miR-633 reduced the proliferation,the number of clones,the wound healing rate,the number of invading cells and the number of cells in the G1 phase of the cell cycle(P0.01).LV-miR-633 also increased the apoptosis rate(P0.01).The expression level of mitogen-activated protein kinase(MAPK)1,high-mobility group box 3(HMGB3),claudin 1(CLDN1)and MAPK13 were downregulated in LV-miR-633-transduced cells(P0.01).The dual luciferase reporter assay confirmed that the 3′-untranslated region of MAPK1 was the t
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