检索结果-南通市图书馆

中国病毒学 2007年第4期22卷 316-325页

作者： Fang-fang ZHUAN Zhen-feng ZHANG Di-ping XU yan-hong si Han-Zhong WANG Ghopur MIJIT College of Life Science and Technology Xinjiang University Urumchi 830046 China State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Institute of Animal and Veterinary Science Academy of Agricultural Sciences of Hubei Wuhan 430064 China

lacZα-mini-attTn7 was inserted into the intergenic region between the gG and gD genes in a PRV bacterial artificial chromosome (BAC) by homologous recombination in E. coli. The resulting recombinant BAC (pBeckerZF1) was confirmed by PCR and sequencing. Green fluorescent protein (GFP) gene was then transposed into pBeckerZF1 by transposon Tn7 to generate pBeckerZF2. Recombinant viruses vBeckerZF1 and vBeckerZF2 were generated by transfection with the corresponding BAC pBeckerZF1 or pBeckerZF2. The titers and cytopathic effect (CPE) observed for by vBeckerZF1 and vBeckerZF2 was comparable to that of the parental virus vBecker3. vBeckerZF2 was serial passaged for five rounds in cell culture, and the mini-Tn7 insertion was stably maintained in viral genome. These results show that recombinant viruses can be rapidly and reliably created by Tn7-mediated transposition. This technology should accelerate greatly the pace at which recombinant PRV can be generated and, thus, facilitate the use of recombinant viruses for detailed mutagenic studies.

关键词：狂犬病病毒无性系 DNA转导 Tn7转座子

Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第39期17卷 4389-4395页

作者： Lin-Lin Gao Fu-Rong Li Peng Jiao Ming-Feng yang Xiao-Jun Zhou yan-hong si Wen-Jian Jiang Ting-Ting Zheng Department of Pathology and Pathophysiology Taishan Medical University Taian 271000 Shandong Province China School of Pharmaceutical Science Taishan Medical University Taian 271000 Shandong Province China Institute of Basic Medical Sciences Taishan Medical University Taian 271000 Shandong Province China Department of Clinical Medicine Taishan Medical University Taian 271000 Shandong Province China

AIM:To investigate the anti-tumor effects of Paris chinensis dioscin(PCD)and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 ***:Cell viability was analyzed by the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide *** apoptosis was evaluated by flow cytometry and laser scanning confocal microscope(LSCM)using Annexin-V/propidium iodide(PI)staining,and the cell cycle was evaluated using PI staining with flow *** calcium ions were detected under fluorescence *** expression of cell cycle and apoptosis-related proteins cyclin B1,CDK1,cytochrome C and caspase-3 was measured by immunohistochemical ***:PCD had an anti-proliferation effect on human gastric cancer SGC-7901 cells in a dose-and time-de-pendent *** treatment of SGC-7901 cells with PCD,apoptosis appeared in SGC-7901 ***-logical changes typical of apoptosis were also observed with LSCM by Annexin V/PI staining,and the cell number of the G0/G1 phase was decreased,while the number of cells in the G2/M phase was *** cycle-related proteins,such as cyclin B1 and CDK1,were all down-regulated,but caspase-3 and cytochrome C were ***,intracellular calcium accumulation occurred in PCD-treated ***:G2/M phase arrest and apoptosis induced by PCD are associated with the inhibition of CDK-activating kinase activity and the activation of Ca2+-related mitochondrion pathway in SGC-7901 cells.

关键词： CyclinB1/CDK1 Cell cycle arrest Caspase-3 Ca2+ Cytochrome C

Research on Bast Fiber Extracted from the White Bark of Three Species in the Genus Broussonetia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2024年第6期42卷 729-738页

作者： Shan-Shan Jin si-Nong Wang yan-yan Huang Jing-Yu Zhang Peng Liu Hui Yu hong-Dong Zhang Yu-Liang yang Institute for Preservation and Conservation of Chinese Ancient Books Fudan University LibraryFudan UniversityShanghai200433China State key laboratory of Molecular Engineering of polymers Department of Macromolecular ScienceFudan UniversityShanghai200433China

Against the backdrop of a global paper resource shortage,there is a growing need to identify fast-growing tree species capable of producing long-lasting *** plant species namely Broussonetia kazinoki,Broussonetia papyrifera and hybrid paper mulberry,belong to the Broussonetia genus,were collected from China to study their white bark suitability for pulp and *** chemical composition revealed that the holocellulose content in Broussonetia kazinoki and Broussonetia papyrifera was more than 80%.The molecular weight distribution of several holocellulose/α-cellulose is observed by GPC,which allows us to better observe the changes of various components on the molecular *** yield,lignin,whiteness,and molecular weight of the pulps obtained by NaOH treatment were *** microscope was used to characterize the fiber length-width ratio and ***,the improvement of the fiber rigidity method based on the Kratky-Porod chain model makes it more theoretical and further reveals the influencing factors of fiber *** study demonstrates the high potentiality of these three species for papermaking applications.

关键词： Broussonetia genus Cellulose Alkaline pulping Kratky-Porod chain model Fiber rigidity

Competitive intermetallics formation in Pd-Zn-Cd system via seeded growth from ultra-thin Pd nanosheets for electrocatalytic ethanol oxidation reaction

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第9期17卷 8552-8559页

作者： Donghao Zhang Lianxi si Qianqian Ren Xintong yan Shi Hu Department of Chemistry School of ScienceTianjin Key Laboratory of Molecular Optoelectronic ScienceTianjin UniversityTianjin 300072China

Pd-based catalysts exhibit higher catalytic activity and durability in many electrochemical ***,the electrochemical performance can be further enhanced by fine-tune of the alloy *** binary alloys have been fully studied,the multicomponent alloys are far beyond understanding,which leaves cocktail effect a compromised explanation for the high-entropy *** Pd nanosheet-seeded growth was used to synthesize a Pd-Zn-Cd ternary alloy by accurately controlling the Pd-Zn-Cd molar ratio through adjusting the amount of introduced Cd *** analysis of the crystal phase structure of PdCdZnx and PdZn_(x)Cd_(1-x),the competitive relationship of Zn and Cd in the alloying process with Pd was unveiled:Pd1Cd1 intermetallics(IMC)is thermodynamically favored over Pd_(1)Zn_(1)IMC in the ternary ***,the increased structure stability of PdCd over PdZn does not bring about increased durability in the catalytic ethanol oxidation *** morphology selection of Pd seeds is also crucial for the study,as Pd cubes,Pd tetrahedrons,and Pd octahedrons do not form PdZn in the same *** successful alloying through the seeded growth depends on the maximum diffusion depth of foreign atoms into the seed.

关键词： PdZn alloy seed growth method ethanol oxidation reaction composition control ternary alloying

Superatom-assembled boranes,carboranes,and low-dimensional boron nanomaterials based on aromatic icosahedral B_(12)and C_(2)B_(10)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2024年第7期17卷 6734-6740页

作者： Qiao-Qiao yan yan-Fang Wei Qiang Chen Yue-Wen Mu si-Dian Li Institute of Molecular Science Shanxi UniversityTaiyuan 030006China

Using the experimentally known aromatic icosahedral superatoms I_(h)B_(12)H_(12)2−and D_(5d)1,12-C_(2)B_(10)H_(12)as building blocks and based on extensive density functional theory calculations,we predict herein a series of core–shell superpolyhedral boranes and carboranes in a bottom-up approach,including the high-symmetry Th B_(12)@B_(152)H_(72)2−(2),C2h C_(2)B_(10)@B_(152)H_(72)(3),D_(3d)B_(12)@B_(144)H_(66)(4),I_(h)B_(12)@C_(24)B_(12)0H_(72)2−(6),and D_(5d)C_(2)B_(10)@C_(24)B_(12)0H_(72)(7).More interestingly,the superatom-assembled linear D2h B_(36)H_(32)^(2−)(8),close-packed planar D_(3d)B_(84)H_(60)^(2−)(10),and nearly close-packed core−shell D_(3d)B_(12)@B144H_(6)6(4)can be extended periodically to form the one-dimensional(1D)α-rhombohedral borane nanowire B_(12)H_(10)(Pmmm)(9),two-dimensional(2D)α-rhombohedral monolayer borophane B_(12)H_(6)(P m1)(11),and the experimentally known three-dimensional(3D)α-rhombohedral boron(R m)(12)which can be viewed as an assembly of the monolayer B_(12)H_(6)(11)staggered in vertical direction,setting up a bottom-up strategy to form low-dimensional boron-based nanomaterials from their borane“seeds”via partial or complete *** bonding analyses indicate that the high stability of these nanostructures originates from the spherical aromaticity of their icosahedral B_(12)or C_(2)B_(10)structural units which possess the universal skeleton electronic configuration of 1S21P61D101F8 following the Wade’s n+1 *** infrared(IR)and Raman spectra of the most-concerned neutral B_(12)@B144H_(6)6(4)and C_(2)B_(10)@C_(24)B_(12)0H_(72)(7)are computationally simulated to facilitate their experimental characterizations.

关键词： superatom-assembling bottom-up approach density functional theory superpolyhedral boranes superpolyhedral carboranes borophanes

Neoadjuvant nivolumab with or without platinum-doublet chemotherapy based on PD-L1 expression in resectable NSCLC(CTONG1804):a multicenter open-label phase II study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

signal Transduction and Targeted Therapy 2024年第1期9卷 249-258页

作者： si-yang Liu Song Dong Xue-Ning yang Ri-Qiang Liao Ben-Yuan Jiang Qun Wang Xiao-Song Ben Gui-Bin Qiao Jun-Tao Lin hong-hong yan Li-Xu yan Qiang Nie Hai-yan Tu Bin-Chao Wang Jin-Ji yang Qing Zhou hong-Rui Li Ke Liu Wendy Wu si-yang Maggie Liu Wen-Zhao Zhong Yi-Long Wu Guangdong Lung Cancer Institute Guangdong Provincial Key Laboratory of Translational Medicine in Lung CancerGuangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouChina Department of Thoracic Surgery Zhongshan HospitalFudan UniversityShanghaiChina Department of Thoracic Surgery Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouChina Department of Pathology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouChina Fujian Key Laboratory of Advanced Technology for Cancer Screening and Early Diagnosis FuzhouChina Berry Oncology Corporation FuzhouChina Chinese Thoracic Oncology Group(CTONG) GuangzhouChina Department of Hematology The First Affiliated HospitalJinan UniversityGuangzhouChina

This prospective multicenter phase II study evaluated the clinical efficacy of neoadjuvant nivolumab-exclusive(N)and nivolumab–chemotherapy(N/C)combinations based on PD-L1 *** patients exhibited resectable clinical stage IIA–IIIB(AJCC 8th edition)NSCLC without EGFR/ALK *** received either mono-nivolumab(N)or nivolumab+nabpaclitaxel+carboplatin(N/C)for three cycles based on PD-L1 *** primary endpoint was the major pathological response(MPR).Key secondary endpoints included the pathologic complete response(pCR),objective response rate(ORR),and event-free survival(EFS).Baseline PD-L1 expression and perioperative circulating tumor DNA(ctDNA)status were correlated with pCR and ***-two patients were enrolled,with 46 undergoing *** MPR was 50.0%(26/52),with 25.0%(13/52)achieving pCR,and 16.7%and 66.7%for patients with PD-L1≥50%in N and N/C groups,***(25.0%)patients experienced grade 3 or higher immune-related adverse events during neoadjuvant *** with post-neoadjuvant ctDNA negativity was more likely to have pCR(39.1%)compared with those remained positive(6.7%,odds ratio=6.14,95%CI 0.84-Inf,p=0.077).With a median follow-up of 25.1 months,the 18-month EFS rate was 64.8%(95%CI 51.9–81.0%).For patients with ctDNA–***+,the 18m-EFS rate was 93.8%vs 47.3%(HR,0.15;95%CI 0.04,0.94;p=0.005).Immunochemotherapy may serve as an optimal neoadjuvant treatment even for patients with PD-L1 expression≥50%.ctDNA negativity following neoadjuvant treatment and surgery could help identify superior pathological and survival benefits,which requires further confirmation in a prospective clinical trial(NCT04015778).

关键词： chemotherapy NSCLC treatment

MiR-142-3p Regulates ILC1s by Targeting HMGB1 via the NF-κB Pathway in a Mouse Model of Early Pregnancy Loss

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current Medical Science 2024年第1期44卷 195-211页

作者： Xiang-li PANG Jie LI Jing WANG si-si yan Jing yanG Department of Obstetrics and Gynecology Renmin Hospital of Wuhan UniversityWuhan 430060China Reproductive Medical Center Renmin Hospital of Wuhan UniversityWuhan 430060China Clinic Research Center for Assisted Reproductive Technology and Embryonic Development in Hubei Province Wuhan 430060China

Objective Innate lymphoid cells(ILCs)are a class of newly discovered *** 1 ILCs(ILC1s)are identified in the decidua of humans and *** mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related ***,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling *** study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling *** Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different *** targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were *** addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,***,changes of the NF-κB signaling pathway in ILC1s were examined in the different *** the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling *** The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all Psignaling pathway(Psignificantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all Psion

关键词： maternal-fetal interface group 1 innate lymphoid cells(ILCis) high mobility group box 1(HMGB1) miR-142-3p abortion

Extreme value theory applied to the auroral electrojet indices

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Earth and Planetary Physics 2024年第2期8卷 375-381页

作者： si Chen hong Yuan Yong Wei Guang yang FengZheng Yu Aerospace Information Research Institute Chinese Academy of SciencesBeijing 100094China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

The study of extreme weather and space events has gained paramount importance in modern society owing to rapid advances in high *** and describing exceptional occurrences plays a crucial role in making decisive assessments of their potential impact on technical,economic,and social aspects in various *** research focuses on analyzing the hourly values of the auroral electrojet(AE)geomagnetic index from 1957 to 2019 by using the peak over threshold method in extreme value *** fitting the generalized Pareto distribution to extreme AE values,shape parameter indices were derived,revealing negative values that establish an upper bound for this time ***,it became evident that the AE values had reached a plateau,suggesting that extreme events exceeding the established upper limit are *** a result,although the need for diligent precautions to mitigate the consequences of such extreme events persists,surpassing the upper limit of AE values becomes increasingly *** is also possible to observe an aurora in the middle-and low-latitude regions during the maximum period of the AE index.

关键词： auroral electrojet indices extreme value theory extreme events

Challenges and Suggestions of Ethical Review on Clinical Research Involving Brain-Computer Interfaces

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2024年第2期39卷 131-139页

作者： Xue-Qin Wang hong-Qiang Sun Jia-Yue si Zi-yan Lin Xiao-Mei Zhai Lin Lu Peking University sixth Hospital Peking University Institute of Mental HealthNHC Key Laboratory of Mental Health(Peking University)National Clinical Research Center for Mental Disorders(Peking University Sixth Hospital)Chinese Academy of Medical Sciences Research Unit(No.2018RU006)Beijing 100191China Department of Psychology University of CaliforniaDavis 95616USA Department of Psychology University of TorontoToronto M5S 1A1Canada School of Population Medicine and Public Health Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Academy for Advanced Interdisciplinary Studies Peking-Tsinghua Centre for Life Sciences and Peking University-International Development Group/McGovern Institute for Brain ResearchPeking UniversityBeijing 100871China

Brain-computer interface(BCI)technology is rapidly advancing in medical research and *** an emerging biomedical engineering technology,it has garnered significant attention in the clinical research of brain disease diagnosis and treatment,neurological rehabilitation,and mental ***,BCI also raises several challenges and ethical concerns in clinical *** this article,the authors investigate and discuss three aspects of BCI in medicine and healthcare:the state of international ethical governance,multidimensional ethical challenges pertaining to BCI in clinical research,and suggestive concerns for ethical *** the great potential of frontier BCI research and development in the field of medical care,the ethical challenges induced by itself and the complexities of clinical research and brain function have put forward new special fields for ethics in *** ensure"responsible innovation"in BCI research in healthcare and medicine,the creation of an ethical global governance framework and system,along with special guidelines for cutting-edge BCI research in medicine,is suggested.

关键词： brain-computer interface clinical research bioethics ethical governance ethical review

Ultrasound blood flow characteristics changes in fetal umbilical artery thrombosis:A retrospective analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第2期12卷 240-248页

作者： si-Jie hong Li-Wei hong Xiao-Qin He Xiao-hong Zhong Department of Ultrasound Women and Children's HospitalSchool of MedicineXiamen UniversityXiamen 361000Fujian ProvinceChina Ministry of Science and Education Women and Children's HospitalSchool of MedicineXiamen UniversityXiamen 361000Fujian ProvinceChina

BACKGROUND Umbilical artery thrombosis(UAT)is extremely uncommon and leads to adverse perinatal *** of blood in pregnant women is suspected to be an important risk for *** is an effective way to detect *** mother can monitor her own fetal health using ultrasound,which enables her to take preventative action in case of *** To investigate ultrasonic blood signal after UAT in the umbilical artery,and evaluate the relationship between hypercoagulability and *** We described a case of a newly formed UAT with markedly altered ultrasonic indices of umbilical artery blood flow,and retrospectively studied it with 18 UAT patients confirmed by histopathology from October 2019 and March 2023 in Xiamen Women and Children's ***’information was collected from medical archives,including maternal clinical data,neonatal outcomes,pathological findings and ultrasonic indices of umbilical artery blood flow,such as systolic-diastolic duration ratio(S/D),resistance index(RI),pulsatility index(PI)and peak systolic velocity(PSV).Ultrasound and coagulation indices were analyzed with matched samples t-test and Wilcoxon rank sum test using the statistical packages in R(version 4.2.1)including car(version 3.1-0)and stats(version 4.2.1),and visualized by ggplot2 package(version 3.3.6).RESULTS A patient with normal findings in second and third-trimester routine ultrasound scan developed UAT with severe changes in ultrasonic indices of umbilical artery blood flow(within 2.5th of reference ranges)in a short period of *** analysis of umbilical artery blood flow ultrasound indices for 19 patients with UAT showed that the decrease in S/D,RI,and PI and increase of PSV during the disease process was greater than that of *** 18 patients delivered in our hospital showed characteristic manifestations of UAT on histological examination after delivery,most of which(16/18)showed umbilical cord abnormalities,with 1

关键词： Umbilical artery thrombosis Obstetric ultrasonography Peak systolic velocity Hypercoagulation Umbilical cord abnormalities Fetus