Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells
Paris chinensis dioscin induces G2/M cell cycle arrest and apoptosis in human gastric cancer SGC-7901 cells作者机构：Department of Pathology and Pathophysiology Taishan Medical University Taian 271000 Shandong Province China School of Pharmaceutical Science Taishan Medical University Taian 271000 Shandong Province China Institute of Basic Medical Sciences Taishan Medical University Taian 271000 Shandong Province China Department of Clinical Medicine Taishan Medical University Taian 271000 Shandong Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2011年第17卷第39期
页 面：4389-4395页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：Supported by The grant from the Department of Education of Shandong Province China No.J10LF18
主 题：细胞周期阻滞 细胞凋亡 薯蓣皂苷 SGC G2 巴黎 胃癌 M期
摘 要：AIM:To investigate the anti-tumor effects of Paris chinensis dioscin(PCD)and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 ***:Cell viability was analyzed by the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide *** apoptosis was evaluated by flow cytometry and laser scanning confocal microscope(LSCM)using Annexin-V/propidium iodide(PI)staining,and the cell cycle was evaluated using PI staining with flow *** calcium ions were detected under fluorescence *** expression of cell cycle and apoptosis-related proteins cyclin B1,CDK1,cytochrome C and caspase-3 was measured by immunohistochemical ***:PCD had an anti-proliferation effect on human gastric cancer SGC-7901 cells in a dose-and time-de-pendent *** treatment of SGC-7901 cells with PCD,apoptosis appeared in SGC-7901 ***-logical changes typical of apoptosis were also observed with LSCM by Annexin V/PI staining,and the cell number of the G0/G1 phase was decreased,while the number of cells in the G2/M phase was *** cycle-related proteins,such as cyclin B1 and CDK1,were all down-regulated,but caspase-3 and cytochrome C were ***,intracellular calcium accumulation occurred in PCD-treated ***:G2/M phase arrest and apoptosis induced by PCD are associated with the inhibition of CDK-activating kinase activity and the activation of Ca2+-related mitochondrion pathway in SGC-7901 cells.
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