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Phase-separated bienzyme compartmentalization as artificial intracellular membraneless organelles for cell repair

Science China Chemistry 2023年第3期66卷 845-852页

作者： yanwen Zhang Shixin wang Yuling yan Xiaoxiao He Zefeng wang Shaohong Zhou Xiaohai yang Kemin wang Jianbo Liu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringKey Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan ProvinceHunan UniversityChangsha 410082China

Implanting artificial organelles in living cells is capable of correcting cellular dysfunctionalities for cell repair and biomedical applications. In this work, phase-separated bienzyme-loaded coacervate microdroplets are established as a model of artificial membraneless organelles in endothelial dysfunctional cells for the cascade enzymatic production of nitric oxide(NO) with a purpose of correcting cellular NO deficiency. We prepared the coacervate microdroplets via liquid-liquid phase separation of oppositely charged polyelectrolytes, in which glucose oxidase/horseradish peroxidase-mediated cascade reaction was compartmented. After the coacervate microdroplets were implanted in NO-deficient dysfunctional cells, the compartments maintained a phase-separated liquid droplet structure, which facilitated a significant enhancement of NO production in the dysfunctional cells. The recovery of NO production was further exploited to inhibit clot formation in blood plasma located in the cell suspension. This demonstrated a proof-of-concept design of artificial organelles in dysfunctional cells for cell repair and anticoagulation-related medical applications. Our results demonstrate an approach for the construction of coacervate droplets through phase separation for the generation of artificial membraneless organelles, which can be designed to provide an array of functionalities in living organisms that have the potential to be used in the field of cell engineering and medical therapy.

关键词： artificial membraneless organelles coacervate microdroplets nitric oxide liquid-liquid phase separation cell repair

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Neutral sulfate berberine modulates cytokine secretion and increases survival in endotoxemic mice

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Acta Pharmacologica Sinica 2006年第9期27卷 1199-1205页

作者： Fei LI Hua-dong wang~2 Da-xiang LU yan-ping wang Ren-bin QI Yong-mei FU Chu-jie LI Department of Pathophysiology,Medical College of Jinan University,Guangzhou 510632,China Department of Pathophysiology Medical College of Jinan University Guangzhou China

Aim:Berberine is thought to be an immunomodulator,so the present study aimedto investigate the effect of berberine on mortality,lung and intestine injury inendotoxemic mice,and the mechanism of its ***:Mice were chal-lenged with lipopolysaccharide(LPS,28 mg/kg,ip),and neutral sulfate berbefinewas administrated *** was monitored every 12 h,and his-tology of the lungs and intestine as well as the plasma tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-12(IL-12),IL-10,and nitric oxide(NO)levels were ***:Pretreatment with 50 mg/kg neutral sulfate ber-berine once a day for 5 days significantly decreased the mortality rate and attenu-ated tissue injury of the lungs and small intestine in mice challenged with *** stimulated a marked increase in plasma levels of TNF-α,IFN-γ,IL-12,IL-10,and *** administration of berberine significantly reduced plasma TNF-α,IFN-γ,and NO levels,but did not suppress plasma IL-12 levels in mice exposed ***,pretreatment with neutral sulfate berbefine augmented IL-10secretion stimulated by LPS in ***:Pretreatment with neutral sul-fate berberine attenuates tissue injury and improves survival in endotoxemic mice,which may be mediated,at least in part,by the inhibition of pro-inflammatorymediator production and upregulation of IL-10 *** findings mightprovide a new strategy for the treatment of endotoxemia.

关键词： lipopolysaccharide berberine tumor necrosis factor-α interleukin-10 interleukin-12 interferon-γ nitric oxide mice

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Ultrasound-triggered release of sinoporphyrin sodium from liposome-microbubble complexes and its enhanced sonodynamic toxicity in breast cancer

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Nano Research 2018年第2期11卷 1038-1056页

作者： Yixiang Li Huanxiao An Xiaobing wang Pan wang Fei Qu yan Jiao Kun Zhang Quanhong Liu Ministry of Education Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China College of Life Sciences Shaanxi Normal University Xi’an China

Applying ultrasound （US） to drug delivery and disease therapy is important work. Sonodynamic therapy （SDT）-a comprehensive therapy using US and a sonosensitizer-exhibits antineoplasfic activity in many tumors. In this stud）5 we investigated the feasibility of using a new sonosensitizer （sinoporphyrin sodium, DVDMS） loaded into liposome-microbubble complexes （DLMBs） as a possible candidate to enhance SDT against breast cancer. DLMBs were synthesized via the biofin-avidin linkage and confirmed to have good US response. US-induced cavitation played a key role to trigger a boosted payload release from DLMBs and improve the cellular uptake and intratumoral diffusion of DVDMS to realize better SDT effect. The combination of DLMBs and US treatment resulted in significant changes to cell morpholog34 mitochondria damage, and cell apoptosis in vitro. In vivo, the combined treatment markedly inhibited tumor growth, which appeared to result from increased apoptosis and reduced proliferation activity. The significant increase in the antitumor effect of DLMBs plus US suggests their potential use as a new approach to promote the killing activity of SDT against breast cancer.

关键词： ultrasound microbubble sinoporphyrin sodium sonodynamic therapy

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Crystallization of Al-based Amorphous Alloys in Good Conductivity Solution

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Journal of Materials Science & Technology 2014年第12期30卷 1262-1270页

作者： Yonggang wang yan Liu Yingjie Li Bang An Guanghui Cao Shifeng Jin Yimin Sun Weimin wang Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials Ministry of Education Shandong University Institute of Materials Science and Engineering Shanghai University Institute of Physics Chinese Academy of Sciences

The corrosion-induced crystallization of Al94 exNixGd6（x=6 and 10, in at.%） metallic glasses as well as phase separation, oxidation and cracking in good conductivity solution has been investigated by various *** transmission electronic microscopy（TEM） result reveals that crystalline intermetallics and oxides present on the electrochemically thinned hole edge, and the phase separation occurs in the matrix of the as-spun ribbons with the circumferential speed Rcof 29.3 m/s. In addition, the bending and cracking of the samples occur after corrosion. The influence of Ni content on the phase separation, bending and cracking can be explained by the fact that the percolation of the backbone clusters in the amorphous alloy melts and glasses is enhanced by increasing the composition of Ni.

关键词： Crystallization Oxidation Percolation of backbone cluster Al-based glassy alloy

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Eye-shaped keyboard for dual-hand text entry in virtual reality

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Virtual Reality & Intelligent Hardware 2023年第5期5卷 451-469页

作者： Kangyu wang yangqiu yan Hao ZHANG Xiaolong LIU Lili wang State Key Laboratory of Virtual Reality Technology and Systems Beihang UniversityBeijing 100083China Peng Cheng Laboratory Shenzhen 518000China

We propose an eye-shaped keyboard for high-speed text entry in virtual reality (VR), having the shape of dual eyes with characters arranged along the curved eyelids, which ensures low density and short spacing of the keys. The eye-shaped keyboard references the QWERTY key sequence, allowing the users to benefit from their experience using the QWERTY keyboard. The user interacts with an eye-shaped keyboard using rays controlled with both the hands. A character can be entered in one step by moving the rays from the inner eye regions to regions of the characters. A high-speed auto-complete system was designed for the eye-shaped keyboard. We conducted a pilot study to determine the optimal parameters, and a user study to compare our eye-shaped keyboard with the QWERTY and circular keyboards. For beginners, the eye-shaped keyboard performed significantly more efficiently and accurately with less task load and hand movement than the circular keyboard. Compared with the QWERTY keyboard, the eye-shaped keyboard is more accurate and significantly reduces hand translation while maintaining similar efficiency. Finally, to evaluate the potential of eye-shaped keyboards, we conducted another user study. In this study, the participants were asked to type continuously for three days using the proposed eye-shaped keyboard, with two sessions per day. In each session, participants were asked to type for 20min, and then their typing performance was tested. The eye-shaped keyboard was proven to be efficient and promising, with an average speed of 19.89 words per minute (WPM) and mean uncorrected error rate of 1.939%. The maximum speed reached 24.97 WPM after six sessions and continued to increase.

关键词： Virtual reality Human computer interaction Text entry Shaped keyboard QWERTY-like key sequence Dual-hand cooperation

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Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

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Biomedical and Environmental Sciences 2016年第2期29卷 127-136页

作者： ZHAO Ran wang Bi CAI Qing Tao LI Xiao Xia LIU Min HU dong GUO dong Bei wang Juan FAN Chun School of Public Health Xiamen University Xiamen 361102 Fujian China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics Xiamen 361102 Fujian China Shanghai Jinshan District Center for Disease Control and Prevention Shanghai 201599 China Department of Preventive Medicine Xiamen Medical School Xiamen 361008 Fujian China

Objective Cr（VI） removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr（VI） bioremediation from sediment samples and to optimize parameters of biodegradation. Methods Strains with the ability to tolerate Cr（VI） were obtained by serial dilution and spread plate methods and characterized by morphology, 16 S r DNA identification, and phylogenetic analysis. Cr（VI） was determined using the 1,5-diphenylcarbazide method, and the optimum p H and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. Results Fifty-five strains were obtained, and one strain（Sporosarcina saromensis M52; patent application number： 201410819443.3） having the ability to tolerate 500 mg Cr（VI）/L was selected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr（VI）/L in 24 h, achieving the highest removal efficiency at p H 7.0-8.5 and 35 °C. Moreover, M52 could completely degrade 100 mg Cr（VI）/L at p H 8.0 and 35 °C in 24 h. The mechanism involved in the reduction of Cr（VI） was considered to be bioreduction rather than absorption. Conclusion The strong degradation ability of S. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation of heavy metal pollution.

关键词： Hexavalent chromium Sediment Sporosarcina saromensis Degradation

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Impacts of warming on root biomass allocation in alpine steppe on the north Tibetan Plateau

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Journal of Mountain Science 2017年第8期14卷 1615-1623页

作者： MA Xing-xing yan yan HONG Jiang-tao LU Xu-yang wang Xiao-dan Key Laboratory of Mountain Surface Processes and Ecological Regulation Institute of Mountain Hazards and EnvironmentChinese Academy of Sciences University of Chinese Academy of Sciences

Biomass is an important component of global carbon cycling and is vulnerable to climate change. Previous studies have mainly focused on the responses of aboveground biomass and phenology to warming, while studies of root architecture and of root biomass allocation between coarse and fine roots have been scarcely reported in grassland ecosystems. We conducted an open-top-chamber warming experiment to investigate the effect of potential warming on root biomass and root allocation in alpine steppe on the north Tibetan Plateau. The results showed that Stipa purpurea had significantly higher total root length, root surface area and tips than Carex moocroftii. However,there were no differences in total root volume, mean diameter and forks for the two species. Warming significantly increased total root biomass(27.60%), root biomass at 0–10 cm depth(27.84%) and coarse root biomass(diameter > 0.20 mm, 57.68%) in the growing season(August). However, warming had no significant influence on root biomass in the non-growing season(April). Root biomass showed clear seasonalvariations: total root biomass, root biomass at 0–10 cm depth and coarse root biomass significantly increased in the growing season. The increase in total root biomass was due to the enhancement of root biomass at 0–10 cm depth, to which the increase of coarse root biomass made a great contribution. This research is of significance for understanding biomass allocation, carbon cycling and biological adaptability in alpine grassland ecosystems under future climate change.

关键词： Coarse root Fine root Root architecture Warming Climate change Open-top chambers

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Growth mechanism of primary Ti_(5)Si_(3) phases in special brasses and their effect on wear resistance

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Journal of Materials Science & Technology 2021年第2期61卷 138-146页

作者： Xianlong wang Jinchuan Jie Shichao Liu Zhuangzhuang dong Guomao Yin Tingju Li Key Laboratory of Solidification Control and Digital Preparation Technology(Liaoning Province) School of Materials Science and EngineeringDalian University of TechnologyDalian116024China

In the present work, in-situ Ti_(5)Si_(3) reinforced special brasses were prepared by melt reaction method. The synthesized Ti_(5)Si_(3) phase shows various morphologies in brasses with different Ti_(5)Si_(3) content, and the3 D morphological evolution of primary Ti_(5)Si_(3)and its growth mechanism were investigated. The Ti_(5)Si_(3) crystal, which bears D8_(8) hexagonal crystal structure, grows along direction and is revealed by{1010} faces during growth. With the increase of Ti_(5)Si_(3) content in the brasses, the morphology of primary Ti_(5)Si_(3)significantly changes from fibers to hexagonal prisms to short-rods with hollow. In addition,the influence of Ti_(5)Si_(3) volume fraction and morphology on the wear behavior of special brass was also revealed. It was substantiated that the wear resistance increases with the increasing volume fraction of Ti_(5)Si_(3), and the corresponding wear mechanism changes from delamination to slight adhesive wear and abrasive wear. However, the friction coefficient shows an abnormal increase when most of the Ti_(5)Si_(3) containing hollows appears in the brass. That is mainly due to the fact that the Ti_(5)Si_(3) is easier to break and fall off resulted by the hollow as a crack source, which makes it unable to resist the plastic deformation of the contact surface during the sliding.

关键词： Ti_(5)Si_(3) Brass Morphology Growth mechanism Wear behavior

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Topography guiding the accelerated and persistently directional cell migration induced by vaccinia virus

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Chinese Chemical Letters 2020年第1期31卷 167-171页

作者： Cheng wang Ji wang Dandan Fu Qiong yan Daiwen Pang Zhiling Zhang Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) College of Chemistry and Molecular Sciences State Key Laboratory of Virology Wuhan University

The rapid transmission of vaccinia virus(VACV)in vivo is thought to be closely related to the cell migration induced by *** migration involved in dynamic changes of cell-substrate adhesion and actin cytoskeleton organization,which can influence by the micro/nano-scale topographic structures that cells are naturally exposed to via contact ***,migration behaviors of VACV-infected cells exposed to topographic cues are still ***,we designed an open chip with microgrooved poly(dimethyl siloxane)(PDMS)substrate to explore the topography roles in VACV-induced cell *** from the random cell migration observed in traditional scratch assay on planar substrate,VACV-infected cells had a tendency to persistently migrate along the axis parallel to microgroove with increased ***,infected cells exhibited a dominant elongated protrusion aligned to the micro-grating axis compare to the shorter lamella extended in any direction on smooth ***,the Golgi complex preferred to relocate behind the nucleus confined within the micro-grating axis in majority of infected migratory *** directional polarization of cells embodied in protrusion formation and Golgi reorientation was responsible for the directionally persistent migration behaviors induced by VACV on microgrooved *** cells response to substrate topography,causing the actin-filled stretched protrusion containing numerous virions and accelerated movement is likely to facilitate direct and rapid spread of *** work opens a window for us to understand the migration behaviors of infected cells in vivo,and also provides a cue for revealing the relationship between virus-induced cell migration and virus rapid spread.

关键词： Cell migration Topography Vaccinia virus Cytoskeleton Contact guidance Micro-patterned substrate

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Application of laparoscopic total mesorectal excision combined with sphincter-preserving surgery in low or ultralow rectal cancer

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Journal of Innovative Optical Health Sciences 2016年第5期9卷 8-14页

作者： yan Liu Xiao-Ming Lu yan-Feng Niu Kai-Xiong Tao Guo-Bin wang Department of Gastrointestinal Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 P.R.China

This study aimed to investigate the influence of laparoscopic total mesorectal excision combined with sphincter-preserving surgery on the postoperative defecation function,urinary function and sexual function in low or ultralow rectal cancer.A retrospective study was undertaken on 107 patients(65 laparoscopic resection and 42 laparoctomic resection)with rectal cancer undergoing laparoscopic or open laparoscopic total mesorectal excision combined with sph incter-preserving surgery from April 2009 to April *** quality of life outcomes of all patients,including defection,urinary and sexual function,were assessed at 6,12 and 24 months after *** quality of life index(GQOLI)was used to evaluate the fecal incont inence and bowel *** and sexual functions were studied by means of questionnaires on the basis of the international prostatic symptom score(IPSS)and international index of erectile function(IEF),*** laparoscopic surgery group,there were two cases of anastomotic leakage,three cases of anast omotic strict ure,seven cases of local recurrence,ten cases of hepatic metastasis and five cases of lung *** satisfaction rates of patients about their defe-cation function reached 60.3%(35/58,84.5%(49/58)and 913%(53/58)at6,12,and 24 months follow-up,*** assessment after one year showed that the overall incidence of uri-nary dysfunction was 10.7%(7/65);Among male patients,18.4%(7/36)sufered from erectile dysfunction and 27.8%(10/36)sufered from ejaculatory dysfunction;65.5%(19/29)female patients investigated were satisfied with their postoperative sexual *** open surgery group,there were two cases of anastomotic leakage,two cases of anastomotic stricture,nine cases of local recurrence,ten cases of hepatic metastasis and seven cases of kung *** satisfaction rates of patients about their defecation function were 56.4%(22/39),82.1%(32/39)and 94.8%(37/39)at 6,12,and 24 months follow-up,resp

关键词： Rectal cancer laparoscope sphincter-preserving surgery quality of life.

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