Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

作     者：ZHAO Ran WANG Bi CAI Qing Tao LI Xiao Xia LIU Min HU Dong GUO Dong Bei WANG Juan FAN Chun 

作者机构：School of Public Health Xiamen University Xiamen 361102 Fujian China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics Xiamen 361102 Fujian China Shanghai Jinshan District Center for Disease Control and Prevention Shanghai 201599 China Department of Preventive Medicine Xiamen Medical School Xiamen 361008 Fujian China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2016年第29卷第2期

页      面：127-136页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the Xiamen Science and Technology Project of China[3502Z20123003] Fundamental Research Funds for the Central Universities of China National Undergraduate Training Program for Innovation and Entrepreneurship 

主　　题：Hexavalent chromium Sediment Sporosarcina saromensis Degradation 

摘      要：Objective Cr（VI） removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr（VI） bioremediation from sediment samples and to optimize parameters of biodegradation. Methods Strains with the ability to tolerate Cr（VI） were obtained by serial dilution and spread plate methods and characterized by morphology, 16 S r DNA identification, and phylogenetic analysis. Cr（VI） was determined using the 1,5-diphenylcarbazide method, and the optimum p H and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. Results Fifty-five strains were obtained, and one strain（Sporosarcina saromensis M52; patent application number： 201410819443.3） having the ability to tolerate 500 mg Cr（VI）/L was selected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr（VI）/L in 24 h, achieving the highest removal efficiency at p H 7.0-8.5 and 35 °C. Moreover, M52 could completely degrade 100 mg Cr（VI）/L at p H 8.0 and 35 °C in 24 h. The mechanism involved in the reduction of Cr（VI） was considered to be bioreduction rather than absorption. Conclusion The strong degradation ability of S. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation of heavy metal pollution.