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Influence of pH, Heat and Enzymatic Treatments on the Activity of Antibacterial Substance in MRS and Milk Media Produced by Lactobacillus fermentum F6

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Agricultural Sciences in China 2010年第6期9卷 911-920页

作者： zhang Yong LIU Yong bao yan zhang He-ping Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education/Department of Food Science and Engineering InnerMongolia Agricultural University Hohhot 010018 P.R. China

This study aimed to investigate the influence of pH,heat and enzymatic treatments on the activity of antibacterial substance in MRS,skim soy milk and bovine milk media fermented by a potential probiotic Lactobacillus fermentum *** antibacterial activity of the culture supernatant of *** F6 was tested against a wide range of Gram-positive and Gram-negative pathogenic bacteria including Staphylococcus aureus,Escherichia coli,Listeria monocytogenes,Salmonella typhimurium,and Shigella *** antibacterial activities were detected in MRS and milk but not in soy *** presumed that three kinds of probable components including organic acids,heat-stable and heat-labile proteinaceous compounds were involved in antibacterial activity of fermented MRS and *** influence of acids on antibacterial activity was pH-dependent and this effect multiplied with thermal treatments seemed *** analyses showed various significant differences of antibacterial activity among five pathogens were observed in pH and heat *** untreated fermented milk showed higher inhibitory activity to Gram-positive pathogenic bacteria than Gram-negative bacteria （P 〈 0.05）,indicating antibacterial substances of fermented milk are greatly different from fermented *** accurate stimulated gastrointestinal transit showed antibacterial substances would have no influence on intestinal *** range of antibacterial substances from pH 2.0 to 6.0 can be potentially used as food biopreservatives and alternative therapeutics.

关键词： Lactobacillusfermentum F6 antibacterial activity pH thermal treatments enzymatic effect

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Cloning of α-β fusion gene from Clostridium perfringens and its expression

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World Journal of Gastroenterology 2006年第8期12卷 1229-1234页

作者： Jia-Ning Bai yan zhang bao-Hua Zhao College of Life Science Hebei Normal University Shijiazhuang 050016 HebeiProvince China

AIM： To study the cloning of α-β fusion gene from Closindium perfringens and the immunogenidty of 0-6 fusion expression. METHODS： Cloning was accomplished after PCR amplification from strains NCTC64609 and C58-1 of the protective antigen genes of α-toxin and β-toxin. The fragment of the gene was cloned using plasmid pZCPAB. This fragment coded for the gene with the stable expression of α-β fusion gene binding. In order to verify the exact location of the α-β fusion gene, domain plasmids were constructed. The two genes were fused into expression vector pBV221. The expressed α-β fusion protein was identified by ELISA, SDS-PAGE, Western blotting and neutralization assay. RESULTS： The protective co-toxin gene （cpa906） and the β-toxin gene （cpb930） were obtained. The recombinant plasmid pZCPAB carrying α-β fusion gene was constructed and transformed into BL21（DE3）. The recombinant strain BL21（DE3）（pZCPAB） was obtained. After the recombinant strain BL21（DE3）（pZCPAB） was induced by 42℃, its expressed product was about 22.14% of total cellular protein at SDS-PAGE and thin-layer gel scanning analysis. Neutralization assay indicated that the antibody induced by immunization with α-βfusion protein could neutralize the toxicity of α-toxin and β-toxin. CONCLUSION： The obtained α-toxin and β-toxin genes are correct. The recombinant strain BL21（DE3）（pZCPAB） could produce α-β fusion protein. This protein can be used for immunization and is immunogenic. The antibody induced by immunization with α-β fusion protein could neutralize the toxicity of α-toxin and β-toxin.

关键词： Clostridium perfringens α-β fusion gene Cloning and expression

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Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第9期11卷 719-727页

作者： Wei WANG Li WANG Xin-xiu LI Xia CHEN Hai-yan zhang Yu HE Jing-jing WANG Yong-yan ZHAO bao-le zhang Yin-xue XU College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China

Bone morphogenetic proteins(BMPs) play a critical role in the growth and steroidogenesis of granulosa cells(GCs).BMP signals act through membrane-bound heteromeric serine/threonine kinase *** ligand binding,BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell *** objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs.A strategy of RNA interference(RNAi)-mediated 'gene silencing' of Smad4,a core molecule mediating the intracellular BMP/Smad signal transduction pathways,was used to interrupt endogenous BMP/Smad *** indicate that Smad4-small interfering RNA(siRNA) caused specific inhibition of Smad4 mRNA and protein expression after *** endogenous BMP/Smad signaling significantly inhibited growth,and induced apoptosis of porcine GCs,while decreasing estradiol *** addition,interrupted BMP/Smad signaling significantly(P<0.05) changed the expression of Cyclin D2,CDK4,Bcl-2,and *** findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.

关键词： Porcine granulosa cells Bone morphogenetic protein(BMP)/Smad Smad4 RNA interference(RNAi) Growth Steroidogenesis

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Effects of water discharge on river-dominated delta growth

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Petroleum Science 2021年第6期18卷 1630-1649页

作者： yan-bao zhang Yi-Ke Liu Jia Yi Xue-Jian Liu Institute of Geophysics China Earthquake AdministrationBeijing100081China Key Laboratory of Petroleum Resources Research Institute of Geology and GeophysicsChinese Academy of SciencesBeijing100029China China Earthquake Disaster Prevention Center China Earthquake AdministrationBeijing100029China The Pennsylvania State University Department of GeosciencesUniversity ParkPA16802USA

River-dominated deltas are commonly developed at modern bays and lakes and ancient petroliferous *** discharge is an important variable at pay zone scales in river-dominated delta reservoirs,which affects deltaic sand distributions and ***,it's unclear how it influences riverdominated delta *** paper integrates Delft3 D simulations and modern analogs to analyze the effects of water discharge,considering growth time,sediment supply,and coupled effects of sediment *** water discharges lead to the formation of lobate deltas,and the water discharge of 1,000 m~3/s is a referenced threshold ***-grained,highly-cohesive sediments increase the threshold values of water discharge at which the deltas become lobate from digitate,and vice *** the same simulation time,high water discharges favor more rugose shorelines,more distributary channels(especially secondary distributaries),and longer and wider deltas with more land ***,for the same sediment supply,high water discharges have few effects on shoreline roughness and the number of distributary channels.

关键词： River-dominated delta Water discharge Morphology Distributary channel Hydrocarbon exploration

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First-order multiples imaging aided by water bottom

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Petroleum Science 2021年第6期18卷 1650-1661页

作者： Zhen-Hua Xu Sheng-He Wu Ming-Cheng Liu Jun-Shou Zhao Zhao-Hui Chen Ke zhang Jia-Jia zhang Zhao Liu State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum-BeijingBeijing 102249China College of Geosciences China University of Petroleum-BeijingBeijing102249China Bohai Petroleum Research Institute CNOOC Tianjin BranchTianjin300459China Sinopec Geophysical Research Institute NanjingJiangsu211103China

Surface-related multiples frequently propagate into the subsurface and contain abundant information on small reflection *** with the conventional migration of primaries,migration of multiples offers complementary illumination and a higher vertical ***,crosstalk artifacts caused by unrelated multiples during reverse time migration(RTM)using multiples severely degrade the reliability and interpretation of the final migration ***,we proposed RTM using first-order receiver-side water-bottom-related multiples for eliminating crosstalk artifacts and enhancing vertical *** first backward propagate the first-order receiver-side water-bottom-related multiples using a water-layer model,followed by saving the upper boundary *** we produce the source wavefield using a seismic wavelet and the receiver wavefield by back-extrapolating the saved ***,the cross-correlation imaging condition is applied to generate the final *** method transforms the receiver-side multiples into primaries,followed by the conventional migration processing *** examples using synthetic datasets demonstrate that our method significantly enhances the imaging quality by eliminating crosstalk artifacts and improving the resolution.

关键词： Surface-related multiples Multiples imaging Multiples elimination

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Visible-light-responsive homologous heterostructure of oxynitrides constructed to boost charge separation and water splitting

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Science China Chemistry 2025年第1期68卷 186-191页

作者： Hai Zou Yunfeng bao Xueshang Xin Yu Qi Tengfeng Xie Fuxiang zhang State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences School of Chemistry and Chemical Engineering Liaoning Normal University College of Chemistry Jilin University

Constructing type II heterostructures represents a widely recognized strategy for enhancing charge separation and photocatalytic efficiency,but it remains challenging to fabricate homologous heterostructures based on visible-light-responsive semiconductors,especially for popularly investigated （oxy）nitrides with low thermal ***,we synthesized a homologous heterostructure （denoted as YTON＿H） based on the oxynitrides of YTaO4-xNyand YTa *** heterostructure exhibits type II band structure alignment and intimate interface achieved through a magnesium （Mg） powder-assisted nitridation *** with widely reported heterologous heterostructures,the as-obtained YTON＿H homologous heterostructure could similarly exhibit enhanced charge separation as well as photocatalytic water splitting performance with respect to its counterparts(YTaO4-xNyand YTaON2).Meanwhile,detailed structural characterizations and spectroscopy analysis have been made to confirm the formation of heterostructure and promotion of spatial charge separation.

关键词： charge separation heterostructure oxynitride photocatalysis visible light

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Autophagy-targeting modulation to promote peripheral nerve regeneration

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Neural Regeneration Research 2025年第7期20卷 1864-1882页

作者： yan Chen Hongxia Deng Nannan zhang Department of Obstetrics and Gynecology West China Second HospitalSichuan UniversityChengduSichuan ProvinceChina Key Laboratory of Birth Defects and Women and Children’s Diseases Ministry of EducationSichuan UniversityChengduSichuan ProvinceChina Laboratory of Reproductive Endocrinology and Reproductive Regulation Sichuan UniversityChengduSichuan ProvinceChina National Center for Birth Defect Monitoring West China Second University HospitalSichuan UniversityChengduSichuan ProvinceChina

Nerve regeneration following traumatic peripheral nerve injuries and neuropathies is a complex process modulated by diverse factors and intricate molecular *** studies have focused on factors that stimulate axonal outgrowth and myelin ***,recent studies have highlighted the pivotal role of autophagy in peripheral nerve regeneration,particularly in the context of traumatic ***,autophagy-targeting modulation has emerged as a promising therapeutic approach to enhancing peripheral nerve *** current understanding suggests that activating autophagy facilitates the rapid clearance of damaged axons and myelin sheaths,thereby enhancing neuronal survival and mitigating injury-induced oxidative stress and *** actions collectively contribute to creating a favorable microenvironment for structural and functional nerve regeneration.A range of autophagyinducing drugs and interventions have demonstrated beneficial effects in alleviating peripheral neuropathy and promoting nerve regeneration in preclinical models of traumatic peripheral nerve *** review delves into the regulation of autophagy in cell types involved in peripheral nerve regeneration,summarizing the potential drugs and interventions that can be harnessed to promote this *** hope that our review will offer novel insights and perspectives on the exploitation of autophagy pathways in the treatment of peripheral nerve injuries and neuropathies.

关键词： autophagy autophagy related genes Charcot–Marie–Tooth diseases diabetic peripheral neuropathy metformin myelination peripheral nerve injury Schwann cells sciatic nerve Wallerian degeneration

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An Artificial Intelligence‑Assisted Flexible and Wearable Mechanoluminescent Strain Sensor System

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Nano-Micro Letters 2025年第3期17卷 217-231页

作者： yan Dong Wenzheng An Zihu Wang Dongzhi zhang College of Control Science and Engineering China University of Petroleum(East China)Qingdao 266580People’s Republic of China

The complex wiring,bulky data collection devices,and difficulty in fast and on-site data interpretation significantly limit the practical application of flexible strain sensors as wearable *** tackle these challenges,this work develops an artificial intelligenceassisted,wireless,flexible,and wearable mechanoluminescent strain sensor system(AIFWMLS)by integration of deep learning neural network-based color data processing system(CDPS)with a sandwich-structured flexible mechanoluminescent sensor(SFLC)*** SFLC film shows remarkable and robust mechanoluminescent performance with a simple structure for easy *** CDPS system can rapidly and accurately extract and interpret the color of the SFLC film to strain values with auto-correction of errors caused by the varying color temperature,which significantly improves the accuracy of the predicted strain.A smart glove mechanoluminescent sensor system demonstrates the great potential of the AIFWMLS system in human gesture ***,the versatile SFLC film can also serve as a encryption *** integration of deep learning neural network-based artificial intelligence and SFLC film provides a promising strategy to break the“color to strain value”bottleneck that hinders the practical application of flexible colorimetric strain sensors,which could promote the development of wearable and flexible strain sensors from laboratory research to consumer markets.

关键词： Mechanoluminescent Strain sensor Flexible Deep learning Wireless

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Modular assembly of Co4-thiacalix[4]arene units and Keggin-type SiW12O40 for visible-light photothermal conversion

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Tungsten 2025年第1期7卷 112-119页

作者： Jia-Hui Li Qing Zhao Dong-Ao Mao Kun Zhou bao-Kuan Chen yan-Feng Bi School of Petrochemical Engineering Liaoning Petrochemical University

The construction of metal-organic coordination clusters（MCCs） using modular secondary building units is an important method for preparing functional *** this study,two new MCCs modularly assembled from Keggin-type {SiW12O40}H4TC4A=p-tert-Butylthiacalix[4]arene(SiW12) units and thiacalixarene-supported Co4-thiacalix[4]arene(Co4-TC4A polynuclear second-building units（PSBUs,H4TC4A=p-tert-Butylthiacalix[4]arene） are *** two MCCs,abbreviated as Co4-SiW12and Co8-SiW12,were structurally characterized by single-crystal X-ray *** Co4-SiW12is a supramolecular structure involving cation-anion interactions between Co4-TC4A PSBUs and ***8-SiW12features "barbell-like" shapes with two Co4-TC4A coordinatively linked to a Keggin-type SiW12unit on opposite via W-O_t-Co *** coordination of Co4-TC4A to SiW12in Co8-SiW12resulted in significantly improved photothermal conversion performances compared with Co4-SiW12and pure SiW12or ***-gap determination and photophysical analysis combined with density functional theory calculations indicated the binding mode between Co4-TC4A PSBUs and SiW12,which considerably influences the way of electron transport and the conversion ability of visible light to heat.

关键词： Modular construction Photothermal conversion Polyoxometalate Thiacalixarene

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Mesh-supported V2O5-WO3/TiO2 nanosheet array catalysts for efficient removal of NOx

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Tungsten 2025年第1期7卷 100-111页

作者： Zhi-Qi zhang Liu yang Wen Luo Vladimir An Jing Li bao-Dan Liu School of Materials Science and Engineering Northeastern University Foshan Graduate School of Innovation Northeastern University Kizhner Research Center School of Advanced Manufacturing Technologies Tomsk Polytechnic University

The design and synthesis of vanadium-based denitration catalysts with low-temperature activity pose significant challenges in the field of toxic gas *** this study,we have developed a novel approach to fabricate highly efficient V2O5-WO3/TiO2monolithic mesh catalysts for NOxreduction at low ***,TiO2nanosheets with strong adhesion and large surface area were grown on Ti mesh using plasma electrolytic oxidation technology and hydrothermal ***,super-small traces of WO3and V2O5active nano-species were subsequently deposited on TiO2nanosheets using the deposition-precipitation *** to traditional ceramic-based V2O5-WO3/TiO2catalysts,our V2O5-WO3/TiO2monolithic mesh catalyst exhibits superior mechanical performance,excellent thermal conductivity,lower pressure drop,higher utilization of active components,and stronger synergistic ***,the V2O5-WO3/TiO2monolithic mesh catalysts exhibited excellent low-temperature catalytic activity with 98% of NOxconversion at 220℃,and remarkable long-term SO2tolerance and water resistance in the selective catalytic reduction（SCR） of NOxwith *** SCR reaction of tiny V2O5and WO3active species supported on TiO2nanosheets directly demonstrated to follow the Eley-Rideal mechanism by in situ infrared spectra.

关键词： Low temperature activity Monolithic mesh catalysts NH3-SCR SO2 tolerance V2O5-WO3/TiO2

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