Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells

作     者：Wei WANG Li WANG Xin-xiu LI Xia CHEN Hai-yan ZHANG Yu HE Jing-jing WANG Yong-yan ZHAO Bao-le ZHANG Yin-xue XU 

作者机构：College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2010年第11卷第9期

页      面：719-727页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0905[农学-畜牧学] 0906[农学-兽医学] 071005[理学-微生物学] 10[医学] 

基　　金：(No. 2006AA10Z136) supported by National High-Tech R & D Program (863) of China 

主　　题：Porcine granulosa cells Bone morphogenetic protein(BMP)/Smad Smad4 RNA interference(RNAi) Growth Steroidogenesis 

摘      要：Bone morphogenetic proteins(BMPs) play a critical role in the growth and steroidogenesis of granulosa cells(GCs).BMP signals act through membrane-bound heteromeric serine/threonine kinase *** ligand binding,BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell *** objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs.A strategy of RNA interference(RNAi)-mediated gene silencing of Smad4,a core molecule mediating the intracellular BMP/Smad signal transduction pathways,was used to interrupt endogenous BMP/Smad *** indicate that Smad4-small interfering RNA(siRNA) caused specific inhibition of Smad4 mRNA and protein expression after *** endogenous BMP/Smad signaling significantly inhibited growth,and induced apoptosis of porcine GCs,while decreasing estradiol *** addition,interrupted BMP/Smad signaling significantly(P0.05) changed the expression of Cyclin D2,CDK4,Bcl-2,and *** findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.