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Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition

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Journal of Zhejiang university-Science B(Biomedicine & Biotechnology) 2008年第7期9卷 558-566页

作者： Yao-xing XU yan-li LI Shao-chun XU Yong liu Xin WANG Jiang-wu TANG Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2.O3, MgSO4, peptone and (NH4)2.O4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each sig-nificant variable, which included urea, Na2.O3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2.(Na2.O3)=?1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.

关键词： Xylanase Optimization of medium composition Plackett-Burman design (PBD) Central composite design (CCD) Response surface methodology (RSM). Aspergillus niger XY- 1

ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis

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Journal of Molecular Cell Biology 2023年第7期15卷 1-14页

作者： Xueyao Ma Mengyuan Li Yi liu Xuefang Zhang Xiaoyun yang Yun Wang Yipeng Li Jiayue Wang Xiuhua liu Zhenzhen yan Xiaochun Yu Chen Wu College of Life Sciences Institute of Life Sciences and Green DevelopmentHebei UniversityBaoding 071002China department of Public Health Affiliated Hospital of Hebei UniversityBaoding 071000China Westlake Laboratory of Life Sciences and Biomedicine Hangzhou 310024China School of Life Sciences Westlake UniversityHangzhou 310024China Institute of Biology Westlake Institute for Advanced StudyHangzhou 310024China

Mono-ADP-ribosylation(MARylation)is a post-translational modification that regulates a variety of biological processes,including DNA damage repair,cell proliferation,metabolism,and stress and immune *** mammals,MARylation is mainly catalyzed by ADP-ribosyltransferases(ARTs),which consist of two groups:ART cholera toxin-like(ARTCs)and ART diphtheria toxin-like(ARTDs,also known as PARPs).The human ARTC(hARTC)family is composed of four members:two active mono-ADP-ARTs(hARTC1 and hARTC5)and two enzymatically inactive enzymes(hARTC3 and hARTC4).In this study,we systematically examined the homology,expression,and localization pattern of the hARTC family,with a particular focus on *** results showed that hARTC3 interacted with hARTC1 and promoted the enzymatic activity of hARTC1 by stabilizing *** also identified vesicle-associated membrane protein-associated protein B(VAPB)as a new target of hARTC1 and pinpointed Arg50 of VAPB as the ADP-ribosylation ***,we demonstrated that knockdown of hARTC1 impaired intracellular calcium homeostasis,highlighting the functional importance of hARTC1-mediated VAPB Arg50 ADP-ribosylation in regulating calcium *** summary,our study identified a new target of hARTC1 in the endoplasmic reticulum and suggested that ARTC1 plays a role in regulating calcium signaling.

关键词： ATRCs mono-ADP-ribosylation hARTC1 VAPB calcium homeostasis

New Metabolic Alterations and A Predictive Marker Pipecolic Acid in Sera for Esophageal Squamous Cell Carcinoma

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Genomics, Proteomics & Bioinformatics 2022年第4期20卷 670-687页

作者： Lei liu Jia Wu Minxin Shi Fengying Wang Haimin Lu Jibing liu Weiqin Chen Guanzhen Yu Dan liu Jing yang Qin Luo yan Ni Xing Jin Xiaoxia Jin Wen-Lian Chen department of Thoracic Surgery The Affiliated Tumor Hospital of Nantong UniversityNantong 226361China Cancer Institute Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China department of Epidemiology Tumor InstituteThe Affiliated Tumor Hospital of Nantong UniversityNantong 226361China department of Clinical Laboratory Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China The Children’s Hospital Zhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310029China department of Pathology The Affiliated Tumor Hospital of Nantong UniversityNantong 226361China

Esophageal squamous cell carcinoma(ESCC)is a major histological subtype of esophageal cancer with a poor *** several serum metabolomic investigations have been reported,ESCC tumor-associated metabolic alterations and predictive biomarkers in sera have not been ***,we enrolled 34 treatment-naive patients with ESCC and collected their pre-and post-esophagectomy sera together with the sera from 34 healthy volunteers for a metabolomic *** comprehensive analysis identified ESCC tumor-associated metabolic alterations as represented by a panel of 12.serum ***,postoperative abrosia and parenteral nutrition substantially perturbed the serum ***,we performed an examination using sera from carcinogen-induced mice at the dysplasia and ESCC stages and identified three ESCC tumor-associated metabolites conserved between mice and ***,among these metabolites,the level of pipecolic acid was observed to be progressively increased in mouse sera from dysplasia to cancerization,and it could be used to accurately discriminate between mice at the dysplasia stage and healthy control ***,this metabolite is essential for ESCC cells to restrain oxidative stress-induced DNA damage and cell proliferation ***,this study revealed a panel of 12.ESCC tumor-associated serum metabolites with potential for monitoring therapeutic efficacy and disease relapse,presented evidence for refining parenteral nutrition composition,and highlighted serum pipecolic acid as an attractive biomarker for predicting ESCC tumorigenesis.

关键词： Esophageal squamous cell carcinoma Serum metabolome Esophagectomy Predictive potential Pipecolic acid

The role of DNA methylation in the maintenance of phenotypic variation induced by grafting chimerism in Brassica

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Horticulture Research 2023年第3期10卷 164-175页

作者： Ke liu Tingjin Wang Duohong Xiao Bin liu {2.g {2.g Kexin Xu Zhenyu Qi {2. Wang Junxing Li Xun Xiang Lu Yuan Liping Chen department of Horticulture College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Agricultural Experiment Station Zhejiang UniversityHangzhou 310058China

Grafting facilitates the interaction between heterologous cells with different genomes,resulting in abundant phenotypic variation,which provides opportunities for crop ***,how grafting-induced variation occurs and is transmitted to progeny remains elusive.A graft chimera,especially a periclinal chimera,which has genetically distinct cell layers throughout the plant,is an excellent model to probe the molecular mechanisms of grafting-induced variation *** we regenerated a plant from the T-cell layer of a periclinal chimera,TCC(where the apical meristem was artificially divided into three cell layers–from outside to inside,L1,L2.and L3;T=Tuber mustard,C=red Cabbage),named rTTT0(r=regenerated).Compared with the control(rsTTT,s=self-grafted),rTTT0 had multiple phenotypic variations,especially leaf shape variation,which could be maintained in sexual *** were analyzed and 58 phenotypic variation-associated genes were ***-genome bisulfite sequencing analyses revealed that the methylome of rTTT0 was changed,and the CG methylation level was significantly increased by 8.74%.In rTTT0,the coding gene bodies are hypermethylated in the CG context,while their promoter regions are hypomethylated in the non-CG *** methylation changes in the leaf shape variation-associated coding genes,ARF10,IAA2.,ROF1,and TPR2.were maintained for five generations of ***,grafting chimerism also affected transcription of the microRNA gene(MIR),among which the DNA methylation levels of the promoters of three MIRs associated with leaf shape variation were changed in rTTT0,and the DNA methylation modification of MIR319 was maintained to the fifth generation of selfed progeny of rTTT0(rTTT5).These findings demonstrate that DNA methylation of coding and non-coding genes plays an important role in heterologous cell interaction-induced variation formation and its transgenerational inheritance.

关键词： phenotypic maintained grafting

Comparison and development of advanced machine learning tools to predict nonalcoholic fatty liver disease:An extended study

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Hepatobiliary & Pancreatic Diseases International 2021年第5期20卷 409-415页

作者： Yuan-Xing liu Xi liu Chao Cen Xin Li Ji-Min liu Zhao-yan Ming Song-Feng Yu Xiao-Feng Tang Lin Zhou Jun Yu Ke-Jie Huang Shu-Sen Zheng department of Hepatobiliary and Pancreatic Surgery the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China College of Information Science and Electrical Engineering Zhejiang UniversityHangzhou 310027China department of Pathology and Molecular Medicine Faculty of Health SciencesMcMaster University1200 Main Street WestHamilton L8S 4K1Canada College of Computer Science and Technology Zhejiang UniversityHangzhou 310027China

Background:Nonalcoholic fatty liver disease(NAFLD)is a public health challenge and significant cause of morbidity and mortality *** identification is crucial for disease *** recently proposed a nomogram-based NAFLD prediction model from a large population *** aimed to explore machine learning tools in predicting ***:A retrospective cross-sectional study was performed on 15315 Chinese subjects(10373 training and 4942.testing sets).Selected clinical and biochemical factors were evaluated by different types of machine learning algorithms to develop and validate seven predictive *** evaluation indicators including area under the receiver operating characteristic curve(AUROC),area under the precision-recall curve(AUPRC),accuracy,positive predictive value,sensitivity,F1 score,Matthews correlation coefficient(MCC),specificity and negative prognostic value were applied to compare the performance among the *** selected clinical and biochemical factors were ranked according to the importance in prediction ***:Totally 4018/10373(38.74%)and 1860/4942.37.64%)subjects had ultrasound-proven NAFLD in the training and testing sets,*** machine learning based models were developed and demonstrated good performance in predicting *** these models,the XGBoost model revealed the highest AUROC(0.873),AUPRC(0.810),accuracy(0.795),positive predictive value(0.806),F1 score(0.695),MCC(0.557),specificity(0.909),demonstrating the best prediction ability among the built *** mass index was the most valuable indicator to predict NAFLD according to the feature ranking ***:The XGBoost model has the best overall prediction ability for diagnosing *** novel machine learning tools provide considerable beneficial potential in NAFLD screening.

关键词： Nonalcoholic fatty liver disease Machine learning Population screening Prediction model Body mass index

Facile generation of highly durable thiol-functionalized polyhedral oligomeric silsesquioxane based superhydrophobic melamine foam

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Frontiers of Chemical Science and Engineering 2022年第8期16卷 1247-1258页

作者： Meng Li Yuanfeng Fang Chun liu Mengmeng Zhou Xiaomei Miao Yongbing Pei Yue yan Wenjun Xiao Huayu Qiu Lianbin Wu Key Laboratory of Organosilicon Chemistry and Materials Technology Ministry of EducationHangzhou Normal UniversityHangzhou 311121China

In this study,a durable superhydrophobic/superoleophilic melamine foam was fabricated by a facile and rapid one-step thiol-ene click chemistry and Michael addition reaction,which demonstrated excellent robustness in oil/water ***,1H,1H,2.-perfluoro-1-hexene reacted with thiol-functionalized polyhedral oligomeric silsesquioxane via the thiol-ene click chemistry to obtain a fluorinated thiol-functionalized polyhedral oligomeric silsesquioxane ***,the melamine foam was immersed to the solution system to form nanoaggregates on the melamine foam surface by the Michael addition reaction in the presence of ultraviolet *** micro/nano rough structure and low surface energy of the nanoaggregates layer endowed the pristine melamine foam with superhydrophobicity;the water contact angle was greater than 150°.More importantly,the as-prepared melamine foam could withstand harsh conditions,such as a corrosive solution environment,strong ultraviolet light,mechanical compression,high and low temperature exposure,and ultrasonic *** by gravity,the as-prepared melamine foam could efficiently separate the oil/water mixtures and maintain 98%separation efficiency at high and low *** addition,it maintained the desirable absorption capability in different oil/organic solvents even after 15 absorption ***,this facile,low-cost,and robust onestep method provides important support for the superhydrophobic oil/water separation field.

关键词： superhydrophobic melamine foam thiol-func tionalized polyhedral oligomeric silsesquioxane durable,oil/water separation

Pediatrics in china: challenges and prospects

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World Journal of Pediatrics 2018年第1期14卷 1-3页

作者： yang liu Li-Li yang Si-Yun Xu Zheng-yan Zhao Editorial Office of World Journal of Pediatrics Children’sHospitalZhejiang University School of MedicineHangzhouChina department of Children’s Health Care Children’s HospitalZhejiang University School of Medicine3333BinshengRoadHangzhou310052China

china has the largest number of children in the world. The population of children between 0 and 14 years of age was about 2.7 million in 2.15. It is a great and arduous task to ensure the healthy growth of all children in{2.. Mater-nal and child health care in{2. has achieved significant improvements.{2. has achieved the Millennium Develop-ment Goal 4 [ 1 ], the mortality rate of children under 5 years of age showed an overall downward trend from 61.0 per 1000 in 1991 to 10.7 per 1000 in 2.15 (2.However,{2. still faces many challenges, such as the serious shortage of pediatricians, shortage and uneven distribution of medical resources, imbalances among different population groups and different regions.

关键词： Pediatrics in china challenges prospects

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老年人定向障碍管理的证据总结

中国护理管理 2024年第5期24卷 719-724页

作者：刘晓玲鲁闻燕赵华吕敏芳余杭青朱英华黄婷妤马燕周妮娅浙江大学医学院附属第二医院护理部杭州市310009

目的:检索、筛选、评价、整合老年人定向障碍管理的相关证据并汇总,为医护人员及照顾者对定向障碍老年人的管理提供循证依据。方法:计算机检索国内外相关数据库、指南网站及相关专业网站中关于老年人定向障碍管理的证据,包括指南、最佳... 详细信息

目的:检索、筛选、评价、整合老年人定向障碍管理的相关证据并汇总,为医护人员及照顾者对定向障碍老年人的管理提供循证依据。方法:计算机检索国内外相关数据库、指南网站及相关专业网站中关于老年人定向障碍管理的证据,包括指南、最佳实践、专家共识、证据总结、系统评价以及随机对照试验。检索时限为建库至2.2.年4月2.。由4名研究者独立对指南进行质量评价、2.研究者独立对其余文献进行质量评价及证据提取、汇总。结果:共纳入9篇文献,包括1篇临床实践指南、2.专家共识、2.系统评价、4篇随机对照试验。最终形成时间定向障碍管理、空间定向障碍管理、人物定向障碍管理以及定向力训练4个方面18条证据。结论:医护人员及照顾者在应用证据时可结合老年人意愿及实际情景,适时推动证据的转化及临床应用,从而降低定向障碍对老年人生活及预后的不良影响。

关键词：老年人定向障碍证据总结循证护理学

Current status and challenge in clinical work of autism spectrum disorders in china

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World Journal of Pediatrics 2018年第3期14卷 209-211页

作者： Zhi-Wei Zhu yan Jin Ling-Ling Wu Xiao-Lin liu department of Developmental and Behavioral Pediatrics Children's HospitalZhejiang University School of MedicineNo.3333 Binsheng RoadHangzhou 310052China

Autism spectrum disorders (ASDs) are a group of neurodevelopmentaldisorders featured by social communicationand interaction deficits, and with limited interests andrepetitive behaviors. In china, ASDs was first reported byTao from Nanjing Brain Hospital in the early 1980s [ 1 , 2.].Since then, more children with autism have been *** the past more than 30 years have witnessed steadygrowth in ASD research and clinical diagnosis and interventionin china, there are still many challenges to *** this article, we will present the current status of prevalence,screening and diagnosis of ASD in china and proposefurther eff orts for practitioners and policy-makers to secureoptimal outcomes for children with ASD.

关键词： Current status challenge