ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis
作者机构：College of Life SciencesInstitute of Life Sciences and Green DevelopmentHebei UniversityBaoding 071002China Department of Public HealthAffiliated Hospital of Hebei UniversityBaoding 071000China Westlake Laboratory of Life Sciences and BiomedicineHangzhou 310024China School of Life SciencesWestlake UniversityHangzhou 310024China Institute of BiologyWestlake Institute for Advanced StudyHangzhou 310024China
出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)
年 卷 期：2023年第15卷第7期
页 面：1-14页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学]
基 金：This work was supported by grants from the National Natural Science Foundation of China(32071277,82002594,81874160,and 32171295) the Natural Science Foundation of Hebei Province(C2021201012) S&T Program of Hebei(216Z2602G) the Interdisciplinary Research Program of Natural Science of Hebei University(DXK202006 and DXK202007) Hebei Natural Science Foundation for Outstanding Young Scholars(H2020201017) the High-level Talents Research Start-up Project of Hebei University(521000981352)
主 题：ATRCs mono-ADP-ribosylation hARTC1 VAPB calcium homeostasis
摘 要：Mono-ADP-ribosylation(MARylation)is a post-translational modification that regulates a variety of biological processes,including DNA damage repair,cell proliferation,metabolism,and stress and immune *** mammals,MARylation is mainly catalyzed by ADP-ribosyltransferases(ARTs),which consist of two groups:ART cholera toxin-like(ARTCs)and ART diphtheria toxin-like(ARTDs,also known as PARPs).The human ARTC(hARTC)family is composed of four members:two active mono-ADP-ARTs(hARTC1 and hARTC5)and two enzymatically inactive enzymes(hARTC3 and hARTC4).In this study,we systematically examined the homology,expression,and localization pattern of the hARTC family,with a particular focus on *** results showed that hARTC3 interacted with hARTC1 and promoted the enzymatic activity of hARTC1 by stabilizing *** also identified vesicle-associated membrane protein-associated protein B(VAPB)as a new target of hARTC1 and pinpointed Arg50 of VAPB as the ADP-ribosylation ***,we demonstrated that knockdown of hARTC1 impaired intracellular calcium homeostasis,highlighting the functional importance of hARTC1-mediated VAPB Arg50 ADP-ribosylation in regulating calcium *** summary,our study identified a new target of hARTC1 in the endoplasmic reticulum and suggested that ARTC1 plays a role in regulating calcium signaling.
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