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琼脂糖微球凝胶制备、物理化学特性及其注射效果

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山东化工 2024年第4期53卷 73-78页

作者：王宝群陈醒李亚利林莎莎甄政安邹圣灿青岛琛蓝海洋生物工程有限公司山东青岛266104

为了解决目前市面上填充剂填充周期短以及注射风险高的问题,以琼脂糖为原料,采用乳化固化法制备出粒径均匀的琼脂糖微球凝胶,并探讨了上述微球凝胶的物理化学特性,评价其作为面部注射填充凝胶的可行性。在高频作用下,进行琼脂糖水溶液... 详细信息

为了解决目前市面上填充剂填充周期短以及注射风险高的问题,以琼脂糖为原料,采用乳化固化法制备出粒径均匀的琼脂糖微球凝胶,并探讨了上述微球凝胶的物理化学特性,评价其作为面部注射填充凝胶的可行性。在高频作用下,进行琼脂糖水溶液与油相的双相均质,得到小粒径琼脂糖微球,碱性条件下与固化剂进行固化反应得到可注射琼脂糖微球凝胶。利用平均粒径在25~40μm之间的小粒径微球进行凝胶制备,正交试验确认反应温度50℃,水浴振荡速率150 r/min,反应时间8 h为最优制备工艺。在30 mm/min条件下检测得到凝胶最大推挤力小于20 N,粘弹性测试结果表明凝胶形成了较为稳定的三维网络结构。同时,细胞毒性和家兔皮下植入后局部反应证明该凝胶作为填充剂具有良好的生物相容性,且降解速率慢,较透明质酸钠凝胶具有更长的填充周期。综合表明,琼脂糖微球凝胶作为一种新型的注射凝胶有望应用于整形美容领域。

关键词：琼脂糖乳化固化法粒径凝胶降解制备生物相容性

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Improving Semantic Part Features for Person Re-identification with Supervised Non-local Similarity

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Tsinghua Science and Technology 2020年第5期25卷 636-646页

作者： Yifan Sun Zhaopeng Dou yali li Shengjin Wang the Department of Electronic Engineering Tsinghua UniversityBeijing 100084China

In person re-IDentification (re-ID) task,the learning of part-level features benefits from fine-grained *** facilitate part alignment,which is a prerequisite for learning part-level features,a popular approach is to detect semantic parts with the use of human parsing or pose *** methods of semantic partition do offer cues to good part alignment but are prone to noisy part detection,especially when they are employed in an off-the-shelf *** response,this paper proposes a novel part feature learning method for re-ID,that suppresses the impact of noisy semantic part detection through Supervised Non-local Similarity (SNS) *** several detected semantic parts,SNS first locates their center points on the convolutional feature maps for use as a set of anchors and then evaluates the similarity values between these anchors and each pixel on the feature *** non-local similarity learning is supervised such that:each anchor should be similar to itself and simultaneously dissimilar to any other anchors,thus yielding the ***,each anchor absorbs features from all of the similar pixels on the convolutional feature maps to generate a corresponding part feature (SNS feature).We evaluate our method with extensive experiments conducted under both holistic and partial re-ID *** results confirm that SNS consistently improves re-ID accuracy using human parsing or pose estimation,and that our results are on par with state-of-the-art methods.

关键词： person re-identification non-local similarity feature learning semantic parts

High-performance nitrogen and sulfur co-doped nanotube-like carbon anodes for sodium ion hybrid capacitors

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Chinese Chemical Letters 2020年第9期31卷 2219-2224页

作者： Yongqiang Ding yali li Junshuai li Xingbin Yan Key Laboratory of Special Function Materials&Structure Design of the Ministry of Education School of Physical Science&TechnologyLanzhou UniversityLanzhou 730000China Laboratory of Clean Energy Chemistry and Materials State Key Laboratory of Solid LubricationLanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China

Sodium ion hybrid capacitors are of great concern in large-scale and cost-effective electrical energy storage owing to their high energy and power densities,as well as natural abundance and wide distribution of ***,it is difficult to find a well-pleasing anode material that matches the high-performance cathode materials to achieve good energy and power output for sodium ion hybrid *** this paper,nitrogen and sulfur co-doped nanotube-like carbon prepared by a simple carbonization process of high sulfur-loaded polyaniline nanotubes is introduced as the *** assembled sodium ion half cell based on the optimal nanotube-like carbon delivers a high reversible capacity of ~304.8 mAh/g at 0.2 A/g and an excellent rate performance of ~124.8 mAh/g at 10 A/g in a voltage window of 0.01-2.5 V(versus sodium/sodium ion).For the hybrid capacitors assembled using the optimal nanotube-like carbon as the anode and high-capacity activated carbon as the cathode,high energy densities of ~100.2 Wh/kg at 250 W/kg and ~50.69 Wh/kg at 12,500 W/kg are achieved.

关键词： Sodium ion hybrid capacitors Anode Nanotube-like carbon Polyaniline nanotubes Electrical energy storage

Research status and prospect of stem cells in the treatment of diabetes mellitus

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Science China(life Sciences) 2013年第4期56卷 306-312页

作者： liU XiaoFang WANG YunFang li yali PEI XueTao Department of Obstetrics and Gynecology General Hospital of PLA Stem Cell and Regenerative Medicine Lab Beijing Institute of Transfusion Medicine Academy of Military Medical Sciences

Beta cell mass and function are decreased to varying degrees in diabetes. Islet cell replacement or regenerative therapy may offer great therapeutic promise to people with diabetes. In addition to primary pancreatic cells, recent studies on regeneration of functional insulin producing cells (IPCs) revealed that several alternative cell sources, including embryonic stem cells, induced pluripotent stem cells and adult stem cells, can generate IPCs by differentiation, reprogramming, and trans-differentiation. In this review, we discuss stem cells as a potential alternative cell source for the treatment of diabetes.

关键词：干细胞治疗糖尿病人展望 IPCS 再生功能细胞来源胚胎干细胞成体干细胞

Design,application and verification of a novel system utilizing bacteria and microalgae to treat swine farm wastewater and produce value-added biomass

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International Journal of Agricultural and Biological Engineering 2023年第4期16卷 222-230页

作者： Xin Zhen yali li Jingwen Qiang Manyu Tang Wei Hua Wanqing Wang Shuang Wu Phil Perkins Roger Ruan Yanling Cheng College of Biochemical Engineering Beijing Union UniversityBeijing 100023China Beijing Key Laboratory of biomass waste resource utilization Beijing 100023 China Solaster LLC NashvilleTN 37210USA Center for Biorefining and Department of Bioproducts and Biosystems EngineeringUniversity of MinnesotaSaint PaulMN 55108USA

Swine farm wastewater is extremely harmful to the environment if not treated before it is *** this study,a system was developed and optimized for testing the high levels of organic matter in swine farm wastewater utilizing a microalgae/bacteria co-culture combined with a novel closed-loop extraction and dilution ***,the system produces biomass that also could be harvested and used in value-added *** efficacy of biomass as a biofertilizer was demonstrated by using a model plant of *** addition,the analysis of biomass indicates that it also has potential as a source for *** a 20-d cultivation period,a yield of biomass was achieved to 2.063 g/L of *** highest removal rates recorded in steady state conditions were:13.8 mg/L·d of Total Nitrogen(TN);11.5 mg/L·d of Ammonia Nitrogen(NH_(4)^(+)-N);24.8 mg/L·d of Chemical Oxygen Demand(COD);and 16.9 mg/L·d of Total Phosphorus(TP).After cultivation,the composition of the biomass was analyzed on a dry basis;the major components were protein(44.9%),lipids(24.6%),carbohydrates(19.9%),Chlorophyll-A(2.75%),Chlorophyll-B(1.66%),and carotenoids(0.57%).This biomass was diluted with water(5%by weight)and used as a biofertilizer to grow *** results showed that the average root and stem lengths of Arabidopsis were 43.0%and 55.0%longer compared to those of the control ***,the number of leaves and the maximum leaf length increased by 30.2%and 39.7%;and the fresh and dry leaf weights increased by 44.0%and 33.7%,*** results demonstrate the efficacy of this system for treating swine farm wastewater whilst simultaneously producing a value-added microalgae/bacteria *** paper also demonstrated the use of biomass as a fertilizer for cultivating a value-added crop and,based on the compositional analyses,propose that the biomass could be used as a raw material for biofuel production due to its high lipid content of 24.6%.By cons

关键词： bacteria-microalgae swine wastewater environmental impact value-added biomass biofertilizer

Effects ofβ-alanine on intestinal development and immune performance of weaned piglets

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Animal Nutrition 2023年第1期12卷 398-408页

作者： linlin Chen Yan Zhong Xiangqin Ouyang Chunfeng Wang Lanmei Yin Jing Huang yali li Qiye Wang Junyan Xie Pengfei Huang Huansheng Yang Yulong Yin National Center of Technology Innovation for Synthetic Biology Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin300308China Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation College of Life SciencesHunan Normal UniversityChangshaHunan410081China Chinese Academy of Science Institute of Subtropical AgricultureResearch Center for Healthy Breeding of Livestock and PoultryChangshaHunan410125China

Beta-alanine is an important amino acid involved in several metabolic reactions in the *** study aimed to investigate the effect ofβ-alanine supplementation on intestinal development and the immune performance of weaned ***-two 21-day-old healthy weaned piglets(half female and half male;Duroc×Landrace×Yorkshire)with an initial body weight of 8.11±0.21 kg were randomly divided into 4 groups with 8 replicates of 1 pig *** control group was fed a basal diet and the three experimental treatment groups were fed diets supplemented with 300,600 and 1,200 mg/kgβ-alanine,*** trial lasted 28 days and the diets fed were divided into 2 phases:the late lactation period(day 1 to 14)and the nursery period(day 15 to 28),during which the weaned piglets had free access to food and *** regulatory effects ofβ-alanine were further investigated in vitro using organoids obtained from the jejunum of *** vivo,the addition ofβ-alanine to the diet had no significant effect on the growth performance of weaned piglets(P>0.05),but significantly reduced serum levels of immunoglobulin G(IgG)(Plin M(IgM)(P=0.005),and complement 3(C3)(P=0.017).The serum interleukin-6(IL-6)levels(P<0.01)were significantly reduced in the 1,200 mg/kg treatment *** addition ofβ-alanine increased ileal villus height,with the most significant effect at a concentration of 300 mg/kg(P=0.041).The addition of 600 mg/kgβ-alanine significantly up-regulated the expression of superoxide dismutase(SOD)activity(P=0.020)and the zonula occludens-1(ZO-1)gene(P=0.049)in the *** supplemented with 300 mg/kgβ-alanine significantly increased the number of Ki67 positive cells in the jejunal crypts(P<0.01).In vitro,β-alanine increased the organoid budding rates(P=0.001)and the budding height of the crypt significantly(P=0.004).In conclusion,β-alanine can improve intestinal morphology and barrier function,reduce inflammatory responses and alleviate the adverse ef

关键词： Weaned piglet Beta-alanine Intestinal development Immune performance

一株人参内生真菌的鉴定及对人参根腐病的防效

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东北农业科学 2024年第1期49卷 80-84页

作者：张晶晶张宁侯微曲正义李亚丽郑培和中国农业科学院特产研究所长春130112

对从人参根部分离得到的1株优势内生真菌(编号为MS-01)进行鉴定并研究其对人参根腐病的防效。结合形态学观察及分子生物学的方法对该菌株进行分析鉴定。确定该菌为毛壳菌属的近缘毛壳菌(Chaetomium subaffine)。对峙培养和发酵液抑菌试... 详细信息

对从人参根部分离得到的1株优势内生真菌(编号为MS-01)进行鉴定并研究其对人参根腐病的防效。结合形态学观察及分子生物学的方法对该菌株进行分析鉴定。确定该菌为毛壳菌属的近缘毛壳菌(Chaetomium subaffine)。对峙培养和发酵液抑菌试验结果表明,该菌株对人参根腐病菌具有良好的抑制作用,抑菌率为(61.57±0.68)%,且在该菌株与人参根腐病菌对峙培养的菌丝交界处,人参根腐病菌菌丝出现溶解和断裂。未灭菌的MS-01菌株发酵液对人参根腐病菌的抑菌率为(79.92±0.77)%;灭菌的MS-01菌株发酵液对人参根腐病菌的抑菌率为(47.22±0.39)%。室内盆栽防治试验结果表明,该菌株对人参根腐病具有一定的防治效果。本研究为人参根腐病的生物防治提供了理论基础。

关键词：内生真菌毛壳菌属近缘毛壳菌生物防治

5-hydroxymethyl-2-furfural prolongs survival and inhibits oxidative stress in a mouse model of forebrain ischemia

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Neural Regeneration Research 2012年第22期7卷 1722-1728页

作者： Bailiu Ya Lan Zhang li Zhang yali li lin li Department of Pharmacology Xuanwu Hospital of Capital Medical University Beijing Geriatric Medical Research Center Key Laboratory for Neurodegenerative Diseases of Ministry of Education Beijing 100053 China Department of Physiology Jining Medical University Jining 272067 Shandong Province China

In the present study, we hypothesized that 5-hydroxymethyl-2-furfural could attenuate ischemic brain damage by reducing oxidative injury. Thus, mice were subjected to bilateral common carotid artery occlusion to establish a model of permanent forebrain ischemia. The mice were intraperitoneally injected with 5-hydroxymethyl-2-furfura130 minutes before ischemia or 5 minutes after ischemia. The survival time of mice injected with 5-hydroxymethyl-2-furfural was longer compared with untreated mice. The mice subjected to ischemia for 30 minutes and reperfusion for 5 minutes were intraperitoneally injected with 5-hydroxymethyl-2-furfural 5 minutes prior to reperfusion, which increased superoxide dismutase content and reduced malondialdehyde content, similar to the effects of Edaravone, a hydroxyl radical scavenger used for the treatment of stroke. These findings indicate that intraperitoneal injection of 5-hydroxymethyl-2-furfural can prolong the survival of mice with permanent forebrain ischemia. This outcome may be mediated by its antioxidative effects.

关键词： 5-hydroxymethyl-2-furfural forebrain ischemia survival time oxidative stress superoxidedismutase malondialdehyde mouse

Mutations of p53 gene exons 4-8 in human esophageal cancer

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World Journal of Gastroenterology 2005年第19期11卷 2998-3001页

作者： li-yali Jin-TianTang li-QunJia Pei-Wenli DepartmentofOncology China-JapanFriendshipHospitalBeijing100029China InstituteofMedicalPhysicsandEngineering TsinghuaUniversityBeijing100084China

AIM: To characterize the tumor suppressor gene p53 mutations in exon 4, esophageal cancer and adjacent noncancerous ***: We performed p53 (exons 4-8) gene mutation analysis on 24 surgically resected human esophageal cancer specimens by PCR, single-strand conformation polymorphism, and DNA sequencing. RESULTS: p53 gene mutations were detected in 9 of 22 (40.9%) esophageal cancer specimens and 10 of 17 (58.8%) adjacent non-cancerous tissues. Eight of sixteen (50.0%) point mutations detected were G-A transitions and 9 of 18 (50.0%) p53 gene mutations occurred in exon 4 in esophageal cancer specimens. Only 1 of 11 mutations detected was G-A transition and 4 of 11 (36.4%) p53 gene mutations occurred in exon 4 in adjacent non-cancerous ***: Mutation of p53 gene in exon 4 may play an important role in development of esophageal cancer. The observation of p53 gene mutation in adjacent noncancerous tissues suggests that p53 gene mutation may be an early event in esophageal carcinogenesis. Some clinical factors, including age, sex, pre-operation therapy and location of tumors, do not influence p53 gene mutation rates.

关键词： p53 基因突变食管癌病理机制基因表达