检索结果-南通市图书馆

Optimizing diabetic kidney disease animal models:Insights from a meta-analytic approach

在线全文

学校读者我要写书评

暂无评论

Animal Models and Experimental Medicine 2023年第5期6卷 433-451页

作者： Fanghong Li Zhi Ma yajie cai Jingwei Zhou Runping Liu School of Chinese Materia Medica Beijing University of Chinese MedicineBeijingChina School of Life Sciences Beijing University of Chinese MedicineBeijingChina Department of Nephrology Dongzhimen HospitalThe First Affiliated Hospital of Beijing University of Chinese MedicineBeijingChina

Diabetic kidney disease(DKD)is a prevalent complication of diabetes,often leading to end-stage renal *** models have been widely used to study the pathogenesis of DKD and evaluate potential ***,current animal models often fail to fully capture the pathological characteristics of renal injury observed in clinical patients with ***,modeling DKD is often a time-consuming,costly,and labor-intensive *** current review aims to summarize modeling strategies in the establishment of DKD animal models by utilizing meta-analysis related methods and to aid in the optimization of these models for future research.A total of 1215 articles were retrieved with the keywords of“diabetic kidney disease”and“animal experiment”in the past 10 *** screening,84 articles were selected for inclusion in the *** manager 5.4.1 was employed to analyze the changes in blood glucose,glycosylated hemoglobin,total cholesterol,triglyceride,serum creatinine,blood urea nitrogen,and urinary albumin excretion rate in each *** lesions shown in different models that were not suitable to be included in the metaanalysis were also extensively *** above analysis suggested that combining various stimuli or introducing additional renal injuries to current models would be a promising avenue to overcome existing challenges and *** conclusion,our review article provides an in-depth analysis of the limitations in current DKD animal models and proposes strategies for improving the accuracy and reliability of these models that will inspire future research efforts in the DKD research field.

关键词： animal model diabetic kidney diseases meta-analysis

Integrative lipidomic and transcriptomic study unravels the therapeutic effects of saikosaponins A and D on non-alcoholic fatty liver disease

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第11期11卷 3527-3541页

作者： Xiaojiaoyang Li Junde Ge Yajing Li yajie cai Qi Zheng Nana Huang Yiqing Gu Qi Han Yunqian Li Rong Sun Runping Liu Beijing University of Chinese Medicine Beijing 100029China The Second Hospital of Shandong University Shandong UniversityJi'nan 250033China Advanced Medical Research Institute Shandong UniversityJi'nan 250012China

Nonalcoholic fatty liver disease(NAFLD)has become one of the most prominent causes of chronic liver diseases and ***,few therapy has been *** Bupleuri(RB)is the most frequently used herbal medicine for the treatment of liver *** the current study,we aim to systemically evaluate the therapeutic effects of saikosaponin A(SSa)and saikosaponin D(SSd),the major bioactive monomers in RB,against NAFLD and to investigate the underlying *** results demonstrated that both SSa and SSd improved diet-induced *** lipidomic and transcriptomic analysis revealed that SSa and SSd modulated glycerolipid metabolism by regulating related genes,like Lipe and *** profoundly suppressed the fatty acid biosynthesis by downregulating Fasn and Acaca expression and promoted fatty acid degradation by inducing Acox1 and Cpt1 a *** analysis further predicted the implication of master transcription factors,including peroxisome proliferator-activated receptor alpha(PPARα),in the protective effects of SSa and *** results were further confirmed in vitro in mouse primary *** summary,our study uncoded the complicated mechanisms underlying the promising anti-steatosis activities of saikosaponins(SSs),and provided critical evidence inspiring the discovery of innovative therapies based on SSa and SSd for the treatment of NAFLD and related complications.

关键词： Saikosaponin Nonalcoholic fatty liver disease Lipidome Transcriptome Lipid metabolism

A novel pulmonary fibrosis murine model with immune-related liver injury

在线全文

学校读者我要写书评

暂无评论

Animal Models and Experimental Medicine 2023年第3期6卷 274-282页

作者： Kexin Jia Jianzhi Wu Yijie Li Jia Liu Runping Liu yajie cai Yinhao Zhang Xiaojiaoyang Li School of Life Sciences Beijing University of Chinese MedicineBeijingChina School of Chinese Materia Medica Beijing University of Chinese MedicineBeijingChina

Idiopathic pulmonary fibrosis(IPF),characterized by aggravated alveolar destruc-tion and fibrotic matrix deposition,tendentiously experiences the stage called acute exacerbation IPF(AE-IPF)and progresses to multiple organ damage,especially liver *** studies have found a variety of immune microenvironment disorders associated with elevated IPF risk and secondary organ injury,whereas current animal models induced with bleomycin(BLM)could not completely reflect the pathologi-cal manifestations of AE-IPF patients in clinic,and the exact underlying mechanisms are not yet fully *** the current study,we established an AE-IPF model by tracheal administration of a single dose of BLM and then repeated administrations of lipopolysaccharide in *** mouse model successfully recapitulated the clinical features of AE-IPF,including excessive intrapulmonary inflammation and fibrosis and extrapulmonary manifestations,as indicated by significant upregulation of Il6,Tnfa,Il1b,Tgfb,fibronectin,and Col1a1 in both lungs and liver and elevated serum aspartate transaminase and alanine transaminase *** effects might be attributed to the regulation of Th17 *** sharing this novel murine model,we expect to pro-vide an appropriate experimental platform to investigate the pathogenesis of AE-IPF coupled with liver injury and contribute to the discovery and development of targeted interventions.

关键词： bleomycin idiopathic pulmonary fibrosis lipopolysaccharide liver injury murine model

In vivo immunomodulatory alleviating effects of animal milk oligosaccharides on murine NEC: A study

在线全文

学校读者我要写书评

暂无评论

FOOD BIOSCIENCE 2023年第3期53卷 1452-1461页

作者： Wu, Junrui Ding, Ruixue Qi, Shuyi Zhang, Shuang Zhao, Liang Xiao, Guanghui cai, yajie Wang, Hanchen Mao, Xueying Wu, Rina Shenyang Agr Univ Coll Food Sci Liaoning Engn Res Ctr Food Fermentat Technol Shenyang Key Lab Microbial Fermentat Technol Innov Shenyang 110866 Peoples R China China Agr Univ Coll Food Sci & Nutr Engn Key Lab Funct Dairy Minist Educ Beijing Peoples R China Heilongjiang Feihe Dairy Co LTD Qiqihar 161033 Peoples R China

Human milk oligosaccharides may mediate prebiotic and anti-inflammatory effects on newborns. It is essential for preterm infants susceptible to intestinal dysfunction and necrotizing enterocolitis (NEC). However, the infant breastfeeding rate remains low, and there is still a great demand for suitable substitutes for HMOs prebiotic effect. In this paper, we study the new molecular insights of the protective mechanism of four animal milk ol-igosaccharides against developing experimental NEC from the perspective of intestinal flora balance and immunomodulatory mechanism. Studies have found that milk oligosaccharides (MOs), especially yak milk oli-gosaccharides, can directly affect the intestinal barrier by inhibiting the expression of inflammatory factors, restoring the abundance of Bifidobacteria, Bacteroidetes and Lactobacilli, improving the intestinal function, bac-terial colonization and NEC resistance of premature infants after birth. These results emphasize that MOs, a substitute for breastfeeding, have specific preventive and alleviating effects on neonatal NEC.

关键词： NEC HMOs Immunomodulatory Gut microbiota Milk oligosaccharides

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

并行数据库基于多连接的查询优化算法的研究

计算机应用与软件 2012年第12期29卷 182-185页

作者：蔡亚杰赵政文白松肖玉泽西北工业大学计算机学院陕西西安710072

多表连接查询是并行数据库中的一种常用且重要的操作,然而基于传统遗传算法所制定的多表连接查询计划,往往存在查询响应时间长的缺陷。根据无共享并行数据库的特点,将一种新的代价估计模型引入到传统遗传算法中,并对传统遗传算法进行了... 详细信息

多表连接查询是并行数据库中的一种常用且重要的操作,然而基于传统遗传算法所制定的多表连接查询计划,往往存在查询响应时间长的缺陷。根据无共享并行数据库的特点,将一种新的代价估计模型引入到传统遗传算法中,并对传统遗传算法进行了改进。实验证明改进后的遗传算法能制定出更优的查询计划,从而减少多表连接时的查询响应时间。

关键词：并行数据库无共享架构多表连接优化代价估计遗传算法

四逆散方中甘草在巨噬细胞中诱导Ⅰ型干扰素应答的作用与机制研究

在线全文

学校读者我要写书评

暂无评论

药物评价研究 2022年第9期45卷 1716-1724页

作者：李硕蔡雅洁白金钊辜一情刘闰平北京中医药大学中药学院北京100029

目的在巨噬细胞中确定四逆散中具有Ⅰ型干扰素(IFN-Ⅰ)调控作用的中药,并探究其具体作用靶点及主要作用成分。方法通过实时荧光定量PCR(qRT-PCR)技术检测四逆散组方药材柴胡、枳实、白芍、甘草水提物对IFN-Ⅰ通路相关基因表达的影响;... 详细信息

目的在巨噬细胞中确定四逆散中具有Ⅰ型干扰素(IFN-Ⅰ)调控作用的中药,并探究其具体作用靶点及主要作用成分。方法通过实时荧光定量PCR(qRT-PCR)技术检测四逆散组方药材柴胡、枳实、白芍、甘草水提物对IFN-Ⅰ通路相关基因表达的影响;通过CCK-8法检测甘草水提物对巨噬细胞RAW264.7细胞活力的影响;qRT-PCR法检测甘草水提物对IFNα2诱导的干扰素诱导基因(ISGs) mRNA表达水平的影响;蛋白免疫印迹(Western blotting)法检测甘草水提物对IFNα2诱导的JAK1、TYK2、STAT1、STAT2蛋白磷酸化水平的影响;qRT-PCR法检测甘草水提物中单体成分甘草酸、甘草苷、异甘草素、甘草素、18β甘草次酸对IFNα2诱导的ISGs的mRNA表达水平的影响。结果四逆散组方药材柴胡、枳实、白芍各水提物对IFNα2诱导的Isg15和Ifit1表达水平无显著影响,而甘草水提物显著增加了IFNα2诱导的Isg15和Ifit1的基因表达水平(P<0.01、0.001);甘草水提物显著增加了IFNα2诱导的JAK1、TYK2、STAT1、STAT2蛋白磷酸化(P<0.05、0.01、0.001);甘草水提物中18β-甘草次酸能够显著增强IFNα2诱导的Isg15和Ifit1的mRNA表达(P<0.001)。结论甘草水提物能够显著激活JAK-STAT信号通路并诱导IFN-Ⅰ下游ISGs的表达,具有较强的固有免疫激活作用。18β-甘草次酸可能是甘草水提物发挥激活IFN-Ⅰ通路的主要有效成分之一。

关键词：四逆散甘草 Ⅰ型干扰素巨噬细胞免疫调控 JAK-STAT信号通路甘草酸甘草苷异甘草素甘草素 18β-甘草次酸

四逆散改善Mdr2^(-/-)小鼠胆汁淤积性肝纤维化作用

在线全文

学校读者我要写书评

暂无评论

药物评价研究 2024年第2期47卷 291-301页

作者：范桂芳蔡雅洁李方红李宇飞刘闰平北京中医药大学中药学院北京100029

目的研究四逆散对Mdr2(Abcb4)基因缺陷(Mdr2^(-/-))小鼠胆汁淤积性肝纤维化的缓解作用,并探究其作用机制。方法 C57BL/6J小鼠作为对照组;C57BL/6J背景的Mdr2^(-/-)小鼠作为模型小鼠,设模型组和四逆散低、高剂量(按生药量计为3.12、6.2... 详细信息

目的研究四逆散对Mdr2(Abcb4)基因缺陷(Mdr2^(-/-))小鼠胆汁淤积性肝纤维化的缓解作用,并探究其作用机制。方法 C57BL/6J小鼠作为对照组;C57BL/6J背景的Mdr2^(-/-)小鼠作为模型小鼠,设模型组和四逆散低、高剂量(按生药量计为3.12、6.24 g·kg^(-1))组。四逆散组连续3周ig给予四逆散水提物,每天1次,对照组给予纯水。试剂盒法检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆汁酸水平;取肝脏、脾脏称质量,计算肝脏、脾脏系数;结合小鼠肝组织HE染色,Masson染色,纤连蛋白(Fibronectin)、细胞角蛋白19(CK19)的免疫组化染色,明确四逆散对Mdr2^(-/-)小鼠肝纤维化及胆汁淤积的影响。基于小鼠肝脏转录组学测序技术,挖掘四逆散改善Mdr2^(-/-)小鼠肝损伤的作用靶点,并通过实时荧光定量PCR(qRT-PCR)法检测肝纤维化[fibronectin(Fn1)、胶原蛋白1(Col1a1)、角蛋白19(krt19)]、炎症[白细胞介素-1β(Il1β)、Il6、肿瘤坏死因子-α(Tnfα)、一氧化氮合成酶(Inos)]、细胞焦亡[凋亡相关斑点样蛋白(Pycard)、Il18]、胆汁酸合成[细胞色素P450家族成员7A1(Cyp7a1)]及转运[胆盐输出泵(Abcb11)、ATP结合盒转运蛋白(Abcc3)、钠离子-牛磺胆酸共转运蛋白(Slc10a1)]相关基因的转录水平。结果与模型组比较,四逆散高剂量显著降低血清总胆汁酸的水平(P<0.05);明显缓解了Mdr2^(-/-)小鼠肝脏中央静脉及胆管周围炎性细胞的浸润和胶原纤维的沉积,并显著抑制胆管反应的发生。转录组学及qRT-PCR结果共同表明,四逆散下调Mdr2^(-/-)小鼠肝脏纤维化、炎症、细胞焦亡相关基因的转录(P<0.05);同时,四逆散下调胆汁酸合成关键限速酶调控基因Cyp7a1和调控胆汁酸向肝内转运的基因Slc10a1的转录,并上调调控胆汁酸外排的基因Abcb11、Abcc3的转录。结论四逆散能缓解Mdr2^(-/-)小鼠胆汁淤积性肝纤维化,机制可能与其调控炎症反应、细胞焦亡以及胆汁酸的合成和转运有关。

关键词：四逆散胆汁淤积性肝纤维化转录组学炎症细胞焦亡胆汁酸代谢

基于“久病入络”理论探讨痰瘀对鼻鼻窦炎嗅觉障碍的影响

在线全文

学校读者我要写书评

暂无评论

北京中医药大学学报 2023年第8期46卷 1100-1105页

作者：孙唯嘉张路秦玲利朱雪莹蔡丽珍高越陈典蔡雅杰中国中医科学院西苑医院北京100091

鼻鼻窦炎是造成嗅觉障碍的主要病因,且此类嗅觉障碍缠绵难愈,符合“久病入络”理论中慢性迁延性疾病“久”而“顽”的发病规律。中医学认为,络脉病变多凝痰聚瘀,按邪气入络的3个阶段以中西医互参的方法分析其病机演变规律,认为鼻鼻窦炎... 详细信息

鼻鼻窦炎是造成嗅觉障碍的主要病因,且此类嗅觉障碍缠绵难愈,符合“久病入络”理论中慢性迁延性疾病“久”而“顽”的发病规律。中医学认为,络脉病变多凝痰聚瘀,按邪气入络的3个阶段以中西医互参的方法分析其病机演变规律,认为鼻鼻窦炎嗅觉障碍初期多因外邪袭络,痰浊内生;中期多因痰结肺络,凝涩血运;末期多由痰瘀互结,浊毒伤络所致。治疗分别以清浊化痰、活血化瘀、祛邪解毒为原则。此外,鼻鼻窦炎嗅觉障碍存在微循环障碍、炎症因子增多、脂质代谢紊乱、诱导细胞凋亡等现代生物学机制,为从痰瘀认识和诊疗该病提供了科学依据。从痰瘀探讨“久病入络”所致的鼻鼻窦炎嗅觉障碍旨在为临床提供新的中医诊疗思路,为无法满足西医治疗的患者提供更加完善的中医诊疗方案。

关键词：鼻鼻窦炎嗅觉障碍久病入络痰瘀理论探讨

在线全文

学校读者我要写书评

暂无评论

发酵桑叶茶工艺条件优化及其抗氧化活性分析

食品工业科技 2024年第15期45卷 187-194页

作者：蔡亚洁乌日娜李煜穆德伦丁瑞雪王军武俊瑞沈阳农业大学食品学院辽宁沈阳110866 辽宁省沈阳市浑南区农业农村局辽宁沈阳110170 彰武绿阳苗木工程有限公司辽宁阜新123200

为了对发酵桑叶茶工艺条件进行优化并探究桑叶茶经发酵后抗氧化活性的变化,该试验通过响应面分析法确定发酵桑叶茶在发酵温度、发酵时间和黑曲霉接种量3种变量条件下的最佳工艺条件,并测定发酵桑叶茶生物活性物质含量及其抗氧化性。结... 详细信息

为了对发酵桑叶茶工艺条件进行优化并探究桑叶茶经发酵后抗氧化活性的变化,该试验通过响应面分析法确定发酵桑叶茶在发酵温度、发酵时间和黑曲霉接种量3种变量条件下的最佳工艺条件,并测定发酵桑叶茶生物活性物质含量及其抗氧化性。结果表明,最佳工艺条件为发酵时间5 d 16 h,发酵温度30℃,接种量16.55%,此条件下所制发酵桑叶茶感官评分高达90分,且其总黄酮、总多糖、多酚、游离氨基酸、芦丁、槲皮素含量比桑叶绿茶分别升高了14.39%、12.67%、2.90%、3.46%、81.13%、19.35%,还检测到了含量7.55%±0.37%的茶褐素。此外,1/1000(g/mL)浓度下发酵桑叶茶对羟基自由基、DPPH自由基、超氧自由基的清除率和对二价铁离子的螯合率分别是桑叶绿茶的1.16倍、1.07倍、1.06倍和0.82倍,该研究结果为今后桑叶的进一步开发与应用提供理论基础和参考。

关键词：发酵桑叶茶生物活性物质工艺优化抗氧化性