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sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway

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The Crop Journal 2024年第1期12卷 164-176页

作者： Dongjiao Wang Wei Wang Shoujian Zang Liqian Qin Yanlan Liang Peixia Lin yachun su Youxiong Que Key Laboratory of sugarcane Biology and Genetic Breeding Ministry of Agriculture and Rural AffairsKey Laboratory of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National Key Laboratory for Tropical Crop Breeding Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesSanya 572024HainanChina

WRKY transcription factors,transcriptional regulators unique to plants,play an important role in defense response to pathogen ***,the resistance mechanisms of WRKY genes in sugarcane remain *** the present study,gene ontology(GO)enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense *** identified gene ScWRKY4,a classⅡc member of the WRKY gene family,in sugarcane cultivar *** gene was induced by salicylic acid(SA)and methyl jasmonate(MeJA)***,expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smutsusceptible sugarcane cultivars infected with Sporisorium ***,stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani *** and caused down-regulated expression of immune marker-related *** analysis indicated suppressed expression of most JAZ genes in the signal transduction ***4 interacted with ScJAZ13 to repress its *** thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance,most likely through the JA signaling *** present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation.

关键词： Disease resistance Expression profile Transcriptome analysis WRKY transcription factors

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Transcription profile analysis of interaction mechanism between sugarcane and Sporisorium scitamineum using microarray

Transcription profile analysis of interaction mechanism betw...

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2019年中国作物学会学术年会

作者：黄宁 Hui Ling Weihua su yachun su Youxiong Que Key Laboratory of sugarcane Biology and Genetic Breeding (Fujian) Ministry of Agriculture Fujian Agriculture and Forestry University Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization Ministry of Education Fujian Agriculture and Forestry University

【Research background】 sugarcane production is seriously affected by Sporisorium scitamineum infection in the world. Although some excellent resistant breeding materials have been created, the utilization of sugarcane germplasm and the breeding of resistant varieties are still greatly restricted for the complex genetic background of sugarcane and the differentiation of smut race. To solve this problem, much effort should be devoted to underlying the mechanism of sugarcane smut resistance and S. scitamineum virulence. 【Materials and methods】 According to our previous RNA-seq and smut pathogen genome data, the gene expression chip was customized. The transcription profile of gene set, which referred to 2,0392 from sugarcane and the whole CDS sequence(6,621) from S. scitamineum genome, was identified on smut spore injected samples. These samples were from sugarcane smut-susceptible genotype YC71-374 and smut-resistant genotype NCo376. 【Result and analysis】 During the interaction between sugarcane and smut fungus, 6,842 sugarcane genes and 3,569 S. scitamineum genes were differentially expressed. According to the GO and KEGG enrichment analysis, the differential expressed sugarcane genes involved in primary metabolism pathway, MAPK pathway, plant hormone signal transduction pathway and ROS response, the differential expressed S. scitamineum genes mostly located in vesicles, and have activities like transportation, signal transduction and redox. After that, the differential expressed genes from sugarcane and S. scitamineum were selected and used to construct the weighted gene co-expression(WGCNA) network of sugarcane-S. scitamineum interaction. Finally, two sugarcane smut-resistance related genes(vacuolar-sorting receptor, ScVSR;and leucine-rich repeat receptor-like protein kinase, Sc LRR-RLK) and four putative S. scitamineum effector genes, including protein essential during penetration 1(SsPep1), hypothetical cysteine-protease inhibitor(SsE4), conserved hypothetical pro

关键词： sugarcane Sporisorium scitamineum microarray WGCNA resistance gene effector protein

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sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway

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The Crop Journal 2024年第1期

作者： Dongjiao Wang Wei Wang Shoujian Zang Liqian Qin Yanlan Liang Peixia Lin yachun su Youxiong Que

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Transcriptome profile analysis of sugarcane responses to Sporisorium scitaminea infection using Solexa sequencing technology

Transcriptome profile analysis of sugarcane responses to Spo...

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中国作物学会2013年学术年会

作者： Qibin Wu Liping Xu Jinlong Guo yachun su Youxiong Que Key Laboratory of sugarcane Biology and Genetic Breeding Ministry of Agriculture/Fujian Agriculture and Forestry University

To understand the molecular basis of sugarcane-smut interaction,it is important to identify sugarcane genes respond to the pathogen ***-throughput tag-sequencing(tag-seq) analysis by Solexa technology was performed on sugarcane infected with ***,which should have massively increased the amount of data available for transcriptome profile *** mapping to sugarcane EST databases in NCBI, we obtained 2015 differentially expressed genes,of which 1125 were up-regulated and 890 down-regulated by *** ontology(GO) analysis revealed that the differentially expressed genes involve in many cellular *** analysis revealed that metabolic pathways and ribosome function are significantly affected, where up-regulation of expression dominates over *** expression of three candidate genes involved in MAP kinase signaling pathway,ScBAK1(GenBank Accession number:KC857629),ScMapkk (GenBank Accession number:KC857627) and ScGloI(GenBank Accession number:KC857628),was confirmed by reverse transcription polymerase chain reaction(RT-PCR).Real-time quantitative PCR(qRT-PCR) analysis concluded that the expression of these genes were all up-regulated after the infection of *** and may play a role in pathogen response in *** present study provides insights into the molecular mechanism of sugarcane defense to *** infection,leading to a more comprehensive understanding of sugarcane-smut interaction.

关键词： sugarcane Sporisorium scitaminea Smut sugarcane-smut interaction Solexa sequencing Transcriptome profile RT-PCR Real-time quantitative PCR

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Transcriptional insights into the sugarcane-Sorghum mosaic virus interaction using RNA-seq and microarray

Transcriptional insights into the sugarcane-Sorghum mosaic v...

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2019年中国作物学会学术年会

作者：凌辉 Ning Huang Qibin Wu yachun su Weihua su Liping Xu Youxiong Que Key Laboratory of sugarcane Biology and Genetic Breeding Ministry of Agriculture Fujian Agriculture and Forestry University College of Life Sciences Fujian Agriculture and Forestry University

【Research background】 sugarcane mosaic disease is one of the widely seen virus caused disease in sugarcane which is an eco-important crop. A good use of biomolecular and bioinformatics tool in pathogen-related genes identification is needed to ravel the molecular mechanism of sugarcane-mosaic virus interaction. 【Materials and methods】 In this study, a transcriptomic database was achieved from a Sorghum mosaic virus(SrMV)-infected sugarcane sample. Then a customized microarray was designed based on transcriptomic database and was used to analyze the differential transcript profile of sugarcane genes across two susceptible cultivars(ROC22 and FN15) and two resistant cultivars(FN39 and YZ01-1413). 【Result and analysis】 In total, 89,338 unigenes, 481 differentially expressed unigenes and 51 homologous sequences of Potyvirus host interactor(PHI) genes were obtained from RNA-seq data. Among 51 PHIs, the upregulation of a calmodulin-related protein gene and an ethylene-inducible transcription factor gene in two SrMV-resistant and two SrMV susceptible sugarcane cultivars under SrMV infection suggests that these two genes could be used as potential gene knockout targets for creating SrMV incompatible germplasm. Based on microarray data, we found that genes, which were ethylene-responsive transcription factor, jasmonate O-methyltransferase, auxin-responsive protein and two-component response regulator, were differentially expressed inSrMV infected leave. Differential transcription of LRR-RLKs, Lec-RLKs, WRKY, b HLH, and MAPKs also have be found among four sugarcane genotypes. 【Conclusion】Genes encoding heat shock protein 70, chloroplastic rieske Fe/S protein, reticulon homology domain protein and salicylic acid binding protein 3 might be used as the potential markers for identifying the resistance or susceptibility of sugarcane materials to SrMV. And phytohormone(ethylen, jasmonate, auxin and cytokinin) related genes, kinase genes(receptor-like kinase and MAPKs) and transcrip

关键词： sugarcane sugarcane mosaic disease transcriptome microarray differential expression

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Cloning and disease resistance analysis of sugarcane allene oxide synthase gene ScAOS1

Cloning and disease resistance analysis of sugarcane allene ...

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2019年中国作物学会学术年会

作者：孙婷婷 Wenju Wang Wenyue Lou yachun su Youxiong Que Key Laboratory of sugarcane Biology and Genetic Breeding Ministry of Agriculture Fujian Agriculture and Forestry University Key Laboratory of Genetics Breeding and Multiple Utilization of Crops Ministry of EducationCollege of Crop Science Fujian Agriculture and Forestry University

【Research background】 Saccharum spp. is one of the main sugar crops and bioenergy crops. Jasmonates(JAs), as an important signaling molecule, involved in regulating a wide range of plant growth and stress response. Allene oxide synthase(AOS), one of the key enzymes in the JAs biosynthesis pathway, plays an important role in enhancing the adaptability of plants to the external environment, resisting the invasion of pests and diseases, and improving the competitiveness of species. However, the study of AOS gene in sugarcane has not been reported yet. 【Materials and methods】 A novel ScAOS1 gene was isolated from sugarcane. The functional characteristics of ScAOS1 were preliminarily identified by bioinformatics analysis, subcellular localization analysis, tissue-specific expression analysis, and gene expression patterns analysis under different hormones and Sporisorium scitamineum stresses, as well as the transient overexpression and stable genetic transformation in Nicotiana benthamiana. 【Result and analysis】 The open reading frame length of ScAOS1 gene was 1446 bp, which encoding 481 amino acids. Its encoded protein was presumed to be an acid-stable hydrophilic and non-secretory 9/13-AOS protein. subcellular localization showed that ScAOS1::GFP fusion protein was distributed in the nucleus, cell membrane and cytoplasm of tobacco epidermal cells. Prokaryotic expression analysis revealed that the pEZYHb-ScAOS1 recombinant protein could be induced by isopropyl-β-D-thiogalactoside in Escherichia coli BL21(DE3). Tissue-specific expression analysis indicated that ScAOS1 gene was expressed in all organs of ROC22, with the highest expression in roots and the lowest expression in leaves. After infection by S. scitamineum, the ScAOS1 gene transcript did not change significantly in the susceptible variety ROC22, but increased significantly and peaked at 48 h which was 2.64-fold higher than the control in the resistant variety Yacheng 05-179. Moreover, the expression of ScAOS1 ge

关键词： sugarcane allene oxide synthase gene cloning expression analysis disease resistance verification

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A TaqMan real-time PCR assay for detection and quantification of Sporisorium scitamineum in sugarcane

A TaqMan real-time PCR assay for detection and quantificatio...

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中国作物学会2013年学术年会

作者： yachun su Shanshan Wang Jinlong Guo Bantong Xue Liping Xu Youxiong Que Key Laboratory of sugarcane Biology and Genetic Breeding Ministry of Agriculture/Fujian Agriculture and Forestry University

Sporisorium scitamineum is a fungal smut pathogen epidemic in sugarcane producing *** detection and proper identification of the smut are an essential requirement in its management *** this study,we developed a TaqMan real-time PCR assay using specific primers(bEQ-F/bEQ-R) and a TaqMan probe (bEQ-P) which were designed based on the bE(b East mating-type) gene(Genbank Accession No.U61290.1). Standard curves revealed that the new TaqMan real-time PCR procedure has high amplification efficiency when applied to a range of samples,*** pbE DNA,and plant DNA extracted from diseased *** method was more sensitive(a detection limit of 10 ag pbE DNA and 0.8 ng sugarcane genomic DNA) than that of conventional PCR(10 fg and 100 ng,respectively).Reliability was demonstrated through the positive detection of samples collected from artificially-inoculated sugarcane plantlets(FN40).This TaqMan assay was capable of detecting the smut pathogen at the initial stage(12 h) of infection and suitable for inspection of sugarcane pathogen-free seed cane and ***,quantification of pathogen was verified in pathogen challenged buds in different sugarcane *** values and copy numbers showed a significant difference between the varieties of Yacheng05-179(resistant) and "ROC"22(susceptible) suggesting this method can be used for evaluation of smut resistance in different sugarcane *** process of teliospore germination and the curve of mycelial growth was observed in order to get a better determination on the key time of sugarcane-smut *** together,this novel assay can be used as a diagnostic tool for sensitive,accurate,fast and quantitative detection of the pathogen especially for asymptomatic seed cane or plants and evaluation of smut resistance of sugarcane genotypes.

关键词： Saccharum officinarum Sporisorium scitamineum TaqMan real-time PCR Standard curve Detection Quantification

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Cloning and identification of a novel plasma membrane intrinsic protein gene ScPIP2-1 in sugarcane

Cloning and identification of a novel plasma membrane intrin...

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2019年中国作物学会学术年会

作者：汤翰臣 Qing Yu Zhu Li Feng Liu Weihua su Hui Ling yachun su Youxiong Que Key Laboratory of sugarcane Biology and Genetic Breeding Ministry of Agriculture Fujian Agriculture and Forestry University Key Laboratory of Genetics Breeding and Multiple Utilization of Crops Ministry of Education College of Crop Science Fujian Agriculture and Forestry University

【Research background】 sugarcane is the most important sugar crop and a major source of sugar production in the world. In our country, 80% of the sugarcane planting areas are dry sloping fields, which are characterized by low rainfall, uneven rainfall and low soil fertility. They are prone to drought and high salt stress, resulting in the loss of total yield and quality of sugarcane. Aquaporins(AQPs) play an important role in maintaining water balance, regulating plant growth and resisting stress, which can be found in major intrinsic protein(MIP) super family and has explicit classification, including plasma membrane intrinsic proteins(PIPs), tonoplast membrane intrinsic proteins(TIPs), nodulin26-like major intrinsic proteins(NIPs), small and basic intrinsic proteins(SIPs) and X intrinsic proteins(XIPs). PIP2 s, one of the family members of PIPs, was found to be responded to osmotic stress such as water balance, water deficit and salinity. So far, PIP2 s have not been reported in sugarcane. 【Materials and methods】 A full-length cDNA sequence of Sc PIP2-1 was successfully cloned from the Saccharum spp. hybrid(ROC22) by RT-PCR. Preliminary identification of ScPIP2-1 gene function was confirmed by tissue-specific expression analysis, gene expression patterns analysis of ScPIP2-1 under polyethylene glycol(PEG), salt(NaCl) and abscisic acid(ABA) stresses, subcellular localization, and transcriptional activation activity analysis by yeast two hybrid self-activation experiment, gene transient expression analysis under drought and NaCl in Nicotiana benthamiana leaves. 【Result and analysis】 The cDNA length of ScPIP2-1 gene was 1145 bp, containing a 873 bp complete open reading frame and encoding 290 amino acids residues. qRT-PCR analysis showed that ScPIP2-1 was constitutively expressed in all five collected tissues(root, leaf, bud, flesh and peel), mainly accumulated in leaves, roots and buds, and with the highest in buds but the lowest in peels. The expression levels of Sc

关键词： sugarcane aquaporins osmotic stress subcellular localization transcriptional activation activity transient expression88

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A cytosolic glucose-6-phosphat dehydrogenase,ScG6PDH gene,plays a positive role in response to various abiotic stresses in sugarcane

A cytosolic glucose-6-phosphat dehydrogenase,ScG6PDH gene,pl...

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2014年中国作物学会学术年会

作者： Yuting Yang Zhiwei Fu yachun su Guoyin Li Jinlong Guo Youxiong Que Liping Xu Key Laboratory of sugarcane Biology and Genetic Breeding Fujian Agriculture and Forestry UniversityMinistry of Agriculture

Glucose-6-phosphat dehydrogenase(G6PDH) is one of the key enzymes in oxidative pentose phosphate pathway,playing a role in response to the abiotic stresses and ***,a full-length cDNA designed as ScG6 PDH from sugarcane,1,646 bp long with 1,524 bp long ORF encoding 507 amino acid residues,was *** of phylogenetic tree showed ScG6 PDH gene was a member of cytosolic G6 PDH gene family,which was consistent with the subcellular localization experiment result of locating in cytoplasm and on *** on a real-time quantitative RT-PCR under the treatments of salt,drought,heavy metal(CdCl) and low temperature(4 ℃),transcription levels of the ScG6 PDH gene were higher than those without treatments,implying its positive response to abiotic *** measurement of ion conductivity and histochemical assay showed that this ScG6 PDH gene also played a role in the hypersensitive cell death response.

关键词： ScG6PDH subcellular location real-time quantitative RT-PCR ion conductivity histochemical assay abiotic stress.

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miRNA alteration is an important mechanism in sugarcane response to low-temperature environment

miRNA alteration is an important mechanism in sugarcane resp...

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2017年中国作物学会学术年会

作者： Yuting Yang Xu Zhang yachun su Jiake Zou Zhoutao Wang Liping Xu Youxiong Que College of Crop Science Key Laboratory of Sugarcane Biology and Genetic BreedingMinistry of Agriculture and Key Laboratory of Crop Genetics and Breeding and Comprehensive UtilizationMinistry of EducationFujian Agriculture and Forestry University

Background:Cold is a major abiotic stress limiting the production of tropical and subtropical crops in new production ***(Saccharum spp.) originates from the tropics but is cultivated primarily in the sub-tropics where it frequently encounters cold *** regulating plant growth,miRNAs play an important role in environmental ***:In this study,a total of 412 sugarcane miRNAs,including 261 known and 151 novel miRNAs,were obtained from 4 small RNA libraries through the Illumina sequencing *** them,62 exhibited significant differential expression under cold stress,with 34 being upregulated and28 being *** expression of 13 miRNAs and 12 corresponding targets was validated by RT-qPCR,with the majority being consistent with the sequencing *** and KEGG analysis indicated that these miRNAs were involved in stress-related biological *** further investigate the involvement of these miRNAs in tolerance to abiotic stresses,sugarcane miR156 was selected for functional ***-qPCR revealed that miR156 levels increased in sugarcane during cold,salt and drought stress *** benthamiana plants transiently overexpressing miR156 exhibited better growth status,lower ROS levels,higher anthocyanin contents as well as the induction of some cold-responsive genes,suggesting its positive role in the plant cold stress ***:This study provides a global view of the association of miRNA expression with the sugarcane response to cold *** findings have enriched the present miRNA resource and have made an attempt to verify the involvement of miR156 in plant response to cold stress.

关键词： Saccharum spp high-throughput sequencing miR156 cold transcriptome

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