A cytosolic glucose-6-phosphat dehydrogenase,ScG6PDH gene,plays a positive role in response to various abiotic stresses in sugarcane
作者单位：Key Laboratory of Sugarcane Biology and Genetic BreedingFujian Agriculture and Forestry UniversityMinistry of Agriculture
会议名称：《2014年中国作物学会学术年会》
会议日期：2014年
关 键 词：ScG6PDH subcellular location real-time quantitative RT-PCR ion conductivity histochemical assay abiotic stress.
摘 要：Glucose-6-phosphat dehydrogenase(G6PDH) is one of the key enzymes in oxidative pentose phosphate pathway,playing a role in response to the abiotic stresses and ***,a full-length cDNA designed as ScG6 PDH from sugarcane,1,646 bp long with 1,524 bp long ORF encoding 507 amino acid residues,was *** of phylogenetic tree showed ScG6 PDH gene was a member of cytosolic G6 PDH gene family,which was consistent with the subcellular localization experiment result of locating in cytoplasm and on *** on a real-time quantitative RT-PCR under the treatments of salt,drought,heavy metal(CdCl) and low temperature(4 ℃),transcription levels of the ScG6 PDH gene were higher than those without treatments,implying its positive response to abiotic *** measurement of ion conductivity and histochemical assay showed that this ScG6 PDH gene also played a role in the hypersensitive cell death response.