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An epi-allele of SMS causes Sanming dominant genic male sterility in rice

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Science China(life Sciences) 2023年第12期66卷 2701-2710页

作者： yachun Yang Cheng Zhang Hao li Zeyuan Yang Zuntao Xu Dewei Tai Dahu Ni Pengcheng Wei Chengxin Yi Jianbo Yang Yong Ding Rice Research Institute Anhui Academy of Agricultural SciencesHefei 230036China Institute of Health and Medicine Hefei Comprehesive National Science CenterHefei 230031China Ministry of Education Key Laboratory for Membraneless Organelles and Cellular Dynamics School of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China

Male sterility is an important trait in rice for hybrid rice(Oryza sativa)***,the factors involved in dominant male sterility are largely ***,we identified a gene from Sanming dominant genic male sterile rice,named Sanming dominant male sterility(SMS),and reported that an epi-allele of this locus contributes to male *** analysis attributed dominant male sterility to a single locus,SMS,which we characterized using a male-sterile near isogenic line(NIL)of rice cultivar *** SMS locus was heterozygous in the male-sterile 93-11 NIL,containing an epi-allele identical to that in 93-11,and an epi-allele identical to that in rice cultivar Nipponbare,which we refer to as SMS9 and SMSN,***9is silent and hyper-methylated,whereas SMSNis expressed and hypo-methylated in the 93-11 *** SMSNled to male *** in SMS rescued the male sterility of the 93-11 ***,we observed the duplication of SMSN in Nipponbare,but did not observe the duplication of SMS9in ***,these findings suggest that the reduced methylation and enhanced expression of the SMSNepi-allele in the 93-11 NIL is responsible for its role in conferring dominant male sterility.

关键词： male sterility rice epi-allele Sanming DNA methylation

Predicting nitrogen use efficiency,nitrogen loss and dry matter intake of individual dairy cows in late lactation by including mid‑infrared spectra of milk samples

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1287-1299页

作者： Rui Shi Wenqi Lou Bart Ducro Aart van der linden Han A.Mulder Simon J.Oosting Shengli li yachun Wang Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture of ChinaNational Engineering Laboratory of Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Wageningen University&Research Animal Breeding and Genomics P.O.Box 3386700 AH Wageningenthe Netherlands Animal Production System Group Wageningen University&ResearchP.O.Box 3386700 AH Wageningenthe Netherlands

Background Nitrate leaching to groundwater and surface water and ammonia volatilization from dairy farms have negative impacts on the ***,the increasing demand for dairy products will result in more pollution if N losses are not ***,a more efficient,and environmentally friendly production system is needed,in which nitrogen use efficiency(NUE)of dairy cows plays a key *** genetically improve NUE,extensively recorded and cost-effective proxies are essential,which can be obtained by including mid-infrared(MIR)spectra of milk in prediction models for *** study aimed to develop and validate the best prediction model of NUE,nitrogen loss(NL)and dry matter intake(DMI)for individual dairy cows in *** A total of 86 lactating Chinese Holstein cows were used in this *** data editing,704 records were obtained for calibration and *** prediction models with three different machine learning algorithms and three kinds of pre-processed MIR spectra were developed for each *** showed that the coefficient of determination(R2)of the best model in within-herd validation was 0.66 for NUE,0.58 for NL and 0.63 for *** external validation,reasonable prediction results were only observed for NUE,with R2 ranging from 0.58 to 0.63,while the R2 of the other two traits was below *** infrared waves from 973.54 to 988.46 cm−1 and daily milk yield were the most important variables for *** The results showed that individual NUE can be predicted with a moderate accuracy in both within-herd and external *** model of NUE could be used for the datasets that are similar to the calibration *** prediction models for NL and 3-day moving average of DMI(DMI_a)generated lower accuracies in within-herd *** also indicated that information of MIR spectra variables increased the predictive ability of ***,pre-processed MIR spectra do not result in higher acc

关键词： Dairy cow Environment Mid-infrared spectra Nitrogen use efficiency Prediction

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基于多尺度细节增强的面部表情识别方法

电子与信息学报 2019年第11期41卷 2752-2759页

作者：谭小慧李昭伟樊亚春首都师范大学信息工程学院北京100048 北京师范大学虚拟现实应用教育部工程研究中心北京100875 首都师范大学电子系统可靠性与数理交叉学科国家国际科技合作示范型基地北京100048 首都师范大学北京成像理论与技术高精尖创新中心北京100048

人类面部表情是其心理情绪变化的最直观刻画,不同人的面部表情具有很大差异,现有表情识别方法均利用面部统计特征区分不同表情,其缺乏对于人脸细节信息的深度挖掘。根据心理学家对面部行为编码的定义可以看出,人脸的局部细节信息决定了... 详细信息

人类面部表情是其心理情绪变化的最直观刻画,不同人的面部表情具有很大差异,现有表情识别方法均利用面部统计特征区分不同表情,其缺乏对于人脸细节信息的深度挖掘。根据心理学家对面部行为编码的定义可以看出,人脸的局部细节信息决定了其表情意义。因此该文提出一种基于多尺度细节增强的面部表情识别方法,针对面部表情受图像细节影响较大的特点,提出利用高斯金字塔提取图像细节信息,并对图像进行细节增强,从而强化人脸表情信息。针对面部表情的局部性特点,提出利用层次结构的局部梯度特征计算方法,描述面部特征点局部形状特征。最后,使用支持向量机(SVM)对面部表情进行分类。该文在CK+表情数据库中的实验结果表明,该方法不仅验证了图像细节对面部表情识别过程的重要作用,而且在小规模训练数据下也能够得到非常好的识别结果,表情平均识别率达到98.19%。

关键词：表情识别图像金字塔高斯差分细节增强支持向量机

A cytosolic glucose-6-phosphat dehydrogenase,ScG6PDH gene,plays a positive role in response to various abiotic stresses in sugarcane

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A cytosolic glucose-6-phosphat dehydrogenase,ScG6PDH gene,pl...

Cloning and identification of a novel plasma membrane intrinsic protein gene ScPIP2-1 in sugarcane

2014年中国作物学会学术年会

作者： Yuting Yang Zhiwei Fu yachun Su Guoyin li Jinlong Guo Youxiong Que liping Xu Key Laboratory of Sugarcane Biology and Genetic Breeding Fujian Agriculture and Forestry UniversityMinistry of Agriculture

Glucose-6-phosphat dehydrogenase(G6PDH) is one of the key enzymes in oxidative pentose phosphate pathway,playing a role in response to the abiotic stresses and ***,a full-length cDNA designed as ScG6 PDH from sugarcane,1,646 bp long with 1,524 bp long ORF encoding 507 amino acid residues,was *** of phylogenetic tree showed ScG6 PDH gene was a member of cytosolic G6 PDH gene family,which was consistent with the subcellular localization experiment result of locating in cytoplasm and on *** on a real-time quantitative RT-PCR under the treatments of salt,drought,heavy metal(CdCl) and low temperature(4 ℃),transcription levels of the ScG6 PDH gene were higher than those without treatments,implying its positive response to abiotic *** measurement of ion conductivity and histochemical assay showed that this ScG6 PDH gene also played a role in the hypersensitive cell death response.

关键词： ScG6PDH subcellular location real-time quantitative RT-PCR ion conductivity histochemical assay abiotic stress.

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Cloning and identification of a novel plasma membrane intrin...

Rapid Automatized Naming and Rapid Alternating Stimulus Tests in Chinese-Speaking Children

2019年中国作物学会学术年会

作者：汤翰臣 Qing Yu Zhu li Feng liu Weihua Su Hui ling yachun Su Youxiong Que Key Laboratory of Sugarcane Biology and Genetic Breeding Ministry of Agriculture Fujian Agriculture and Forestry University Key Laboratory of Genetics Breeding and Multiple Utilization of Crops Ministry of Education College of Crop Science Fujian Agriculture and Forestry University

【Research background】 Sugarcane is the most important sugar crop and a major source of sugar production in the world. In our country, 80% of the sugarcane planting areas are dry sloping fields, which are characterized by low rainfall, uneven rainfall and low soil fertility. They are prone to drought and high salt stress, resulting in the loss of total yield and quality of sugarcane. Aquaporins(AQPs) play an important role in maintaining water balance, regulating plant growth and resisting stress, which can be found in major intrinsic protein(MIP) super family and has explicit classification, including plasma membrane intrinsic proteins(PIPs), tonoplast membrane intrinsic proteins(TIPs), nodulin26-like major intrinsic proteins(NIPs), small and basic intrinsic proteins(SIPs) and X intrinsic proteins(XIPs). PIP2 s, one of the family members of PIPs, was found to be responded to osmotic stress such as water balance, water deficit and salinity. So far, PIP2 s have not been reported in sugarcane. 【Materials and methods】 A full-length cDNA sequence of Sc PIP2-1 was successfully cloned from the Saccharum spp. hybrid(ROC22) by RT-PCR. Preliminary identification of ScPIP2-1 gene function was confirmed by tissue-specific expression analysis, gene expression patterns analysis of ScPIP2-1 under polyethylene glycol(PEG), salt(NaCl) and abscisic acid(ABA) stresses, subcellular localization, and transcriptional activation activity analysis by yeast two hybrid self-activation experiment, gene transient expression analysis under drought and NaCl in Nicotiana benthamiana leaves. 【Result and analysis】 The cDNA length of ScPIP2-1 gene was 1145 bp, containing a 873 bp complete open reading frame and encoding 290 amino acids residues. qRT-PCR analysis showed that ScPIP2-1 was constitutively expressed in all five collected tissues(root, leaf, bud, flesh and peel), mainly accumulated in leaves, roots and buds, and with the highest in buds but the lowest in peels. The expression levels of Sc

关键词： sugarcane aquaporins osmotic stress subcellular localization transcriptional activation activity transient expression88

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Rapid Automatized Naming and Rapid Alternating Stimulus Test...

Characteristics and main control factors of Ordovician shoal dolomite gas reservoir in Gucheng area,Tarim Basin,NW China

第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Min Zhang Tingting li yachun Xie Qin Hong Yaqin Xu Chunmei Shi lin Chen Xirong Guo Meiling Tong Xia Chi Department of pediatric Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Institute of Pediatrics Nanjing Medical University

Background:The Rapid Automatized Naming and Rapid Alternating Stimulus Tests(RAN/RAS Tests)are important for evaluating the development of reading *** were no established norm exists for using the RAN/RAS Tests on Chinese-speaking Chinese ***:These Objectives were used to develop norms forRAN/RAS Tests for Chinese-speaking children

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Petroleum Exploration and Development 2022年第1期49卷 52-63页

作者： FENG Jun ZHANG Yajin ZHANG Zhenwei FU Xiaofei WANG Haixue WANG yachun liU Yang ZHANG Junlong li Qiang FENG Zihui College of Earth Science Northeast Petroleum UniversityDaqing 163318China Ministry of Education Key Laboratory of Continental Shale Hydrocarbon Accumulation and Efficient Development Northeast Petroleum UniversityDaqing 163318China Exploration and Development Research Institute Daqing Oilfield Company Ltd.Daqing 163712China Heilongjiang Institute of Technology Ha’erbin 150050China

Based on seismic, drilling data and experimental analysis, the characteristics and main controlling factors of shoal dolomite gas reservoir in the third member of Ordovician Yingshan Formation of Gucheng area, Tarim basin were *** study shows that the dolomite gas reservoir in Gucheng area is lithologic gas reservoir controlled by shoal and fault jointly,and its formation is mainly attributed to the following factors:(1) The continuously developing paleotectonic structure has been in the direction of gas migration and accumulation;(2) The large area of medium-high energy grain bank deposited in gentle slope environment is the material basis for the formation of dolomite reservoir;(3) Atmospheric water leaching and dolomitization and fluid dissolution in fault zone are the key factors for the formation of high-quality dolomite reservoir;(4)The natural gas comes from cracking of the ancient oil reservoir and hydrocarbon generation of dispersed organic matter in source rocks, and the NNE-trending strike-slip fault is the dominant channel for vertical migration of natural gas;(5) limestone cap rocks in the first and second members of Yingshan Formation provide direct sealing for the formation of gas reservoir there. On the basis of comprehensive analysis, it is pointed out that the Gucheng area has three grain shoal zones in the third member of Yingshan Formation in nearly S-N direction, which together with seven strike-slip fault zones in NNE direction control the development of shoal dolomite gas reservoir.

关键词： Tarim Basin Gucheng area Ordovician Yingshan Formation dolomite gas reservoir grain shoal facies strike-slip fault dissolution

利塞膦酸钠和阿仑膦酸钠治疗对股骨粗隆下侧皮质骨密度变化的影响对比研究

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中国骨质疏松杂志 2019年第6期25卷 842-846页

作者：钟亚春陆璇徐立武汉市第一医院湖北武汉430022

目的回顾性评估利塞膦酸钠和阿仑膦酸钠治疗对股骨粗隆下侧皮质(subtrochanteric lateral cortex,STLC)骨密度(bone mineral density,BMD)变化的影响。方法共有168例受试者,使用双能X线骨密度仪(dual energy X-ray absorptiometry,DXA)对其BMD进行2~4年间隔随访,纳入本次回顾性研究,分为3组:46例未服用任何抗骨质疏松药物(对照组),69例使用阿仑膦酸钠(阿仑膦酸钠组),53例使用利塞膦酸盐(利塞膦酸盐组)。通过在骨皮质上绘制矩形感兴趣区,在每位患者的STLC和转子下内侧皮质(subtrochanteric medial cortex,STMC)中测量BMD。通过使用协方差模型控制来分析STLC中BMD的百分比变化,以控制年龄、体质量指数、STMC百分比变化、髋轴长度、DXA检查之间的时间间隔的5个独立变量。结果对照组、阿仑膦酸盐组和利塞膦酸盐组BMD的最小均数±标准差分别为1.47±1.51、2.24±1.27和6.98±1.23。与对照组(调整后的P=0.010)或阿仑膦酸盐组(调整后的P=0.011)相比,利塞膦酸盐组的STLC显示出更高的BMD百分比变化。结论利塞膦酸盐组中STLC的BMD变化百分比大于阿仑膦酸盐组和对照组。

关键词：阿仑膦酸钠骨密度股骨利塞膦酸盐骨质疏松

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驱油用纳米材料在提高采收率方面研究进展

化工进展 2024年第9期43卷 5035-5048页

作者：李镇武蒲迪熊亚春吴定莹金诚郭拥军西南石油大学化学化工学院四川成都610500 西南石油大学油气藏地质及开发工程国家重点实验室四川成都610500 四川光亚聚合物化工有限公司四川南充637900

随着对石油资源需求不断上升和开采难度不断增加,提高采收率的技术需要不断创新,纳米材料作为一种新型材料,由于具有纳米尺寸和独特晶界结构,导致其具有独特的性质,在提高采收率方面具有很大的潜力。本文系统综述了纳米材料在提高采收... 详细信息

随着对石油资源需求不断上升和开采难度不断增加,提高采收率的技术需要不断创新,纳米材料作为一种新型材料,由于具有纳米尺寸和独特晶界结构,导致其具有独特的性质,在提高采收率方面具有很大的潜力。本文系统综述了纳米材料在提高采收率方面的作用机理,包括降低表/界面张力,增加毛细管数;结构分离压力作用,微观展示石油从岩石上剥离;改变润湿性,加强水渗吸作用;增强乳液和泡沫稳定性从而增强体系稳定性;改善流度比增加体积波及效率;抑制沥青沉积,催化沥青分解,防止孔隙堵塞和减少地层伤害;抑制颗粒运移、减少地层伤害等。列举了纳米材料在低渗透油藏、稠油油藏和高含水油藏等条件下提高采收率的矿场案例,指出了纳米材料的油田规模化应用所面临的挑战。

关键词：纳米材料石油界面张力驱油机理提高采收率