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Insect Science 2020年第3期27卷 440-448页

作者： Jing Wang ya-yun zuo Ling-Li Li Hui Wang Shao-yan Liu Yi-Hua yang Yi-Dong Wu College of Plant Protection Nanjing Agricultural UniversityNanjingChina

Bacillus thuringiensis(Bt)insecticidal toxins have been globally utilized for control of agricultural insects through spraying or transgenic *** of Bt toxins to special receptors on midgut epithelial cells of target insects is a key step in the mode of *** studies suggested aminopeptidase N1(APN1)as a receptor or putative receptor in several lepidopteran insects including Helicoverpa armigera through evidence from RNA interefence‐based gene silencing *** the current study we tested the role of APNs in the mode of action of Bt toxins using clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR‐associated protein 9‐mediated gene *** APN genes(HaAPN1,HaAPN2 and HaAPN5)were individually knocked out in a susceptible strain(SCD)of *** to establish three homozygous knockout *** in vitro binding studies indicated binding of Cry1Ac or Cry2Ab to midgut brush border membrane vesicles was not obviously affected by APN *** results showed that none of the three knockouts had significant changes in susceptibility to Cry1A or Cry2A toxins when compared with the SCD *** suggests that the three HaAPN genes we tested may not be critical in the mode of action of Cry1A or Cry2A toxins in ***.

关键词： aminopeptidase N Bacillus thuringiensis CRISPR/Cas9 Helicoverpa armigera receptor

Knockin of the G275E mutation of the nicotinic acetylcholine receptor (nAChR) α6 confers high levels of resistance to spinosyns in Spodoptera exigua

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Insect Science 2022年第2期29卷 478-486页

作者： ya-yun zuo Yu-Xin Xue Ze-Yu Wang Xuan Ren Ahmed A.A.Aioub Yi-Dong Wu Yi-Hua yang Zhao-Nong Hu Institute of Pesticide Science College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi712100 China College of Plant Protection Nanjing Agricultural UniversityNanjing210095 China Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi712100 China Key Laboratory for Botanical Pesticide R&D of Shaanxi Province YanglingShaanxi712100 China Plant Protection Department Faculty of AgricultureZagazig UniversityZagazig44511 Egypt

Spinosyns,including spinosad and spinetoram,act on the insect central nervous system,gradually paralyzing or destroying the target *** resistance is associated with loss-of-function mutations in the nicotinic acetylcholine receptor(nAChR)α6 subunit in a number of agricultural *** gene editing,nAChRα6 has been verified as a target for spinosyns in five insect ***,a point mutation(G275E)in exon 9 of nAChRα6 was identified in spinosad-resistant strains of Thrips palmi and Tuta *** date,no in vivo functional evidence has been obtained to support that this mutation is involved in spinosyn resistance in lepidopteran *** this study,the G275E mutation was introduced into the nAChR of Spodoptera exigua using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene-editing *** transcriptase-polymerase chain reaction and sequencing confirmed that this mutation was present in exon 9 of the nAChR transcripts in the edited 275E *** results of bioassays showed that the 275E strain was highly resistant to spinosad(230-fold)and spinetoram(792-fold)compared to the unedited background strain,directly confirming that the G275E mutation of the nAChRα6 subunit confers high levels of spinosyn resistance in *** analysis showed that the resistance trait is autosomal and incompletely *** study employs a reverse genetics approach to validate the functional role played by the G275E mutation in nAChRα6 of *** in spinosyns resistance and provides another example of the use of CRISPR/Cas9 gene-editing technology to confirm the role played by candidate target site mutations in insecticide resistance.

关键词： beet armyworm CRISPR G275E mutation nicotinic acetylcholine recep・tor spinosyns insecticides target resistance

Identification of the ryanodine receptor mutation I4743M and its contribution to diamide insecticide resistance in Spodoptera exigua (Lepidoptera: Noctuidae)

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Insect Science 2020年第4期27卷 791-800页

作者： ya-yun zuo Huan-Huan Ma Wen-Jie Lu Xing-Liang Wang Shu-Wen Wu Ralf Nauen Yi-Dong Wu Yi-Hua yang College of Plant Protection Nanjing Agricultural UniversityNanjingChina Bayer CropScience R&D Pest ControlMonheimGermany

Insect ryanodine receptors(RyRs)are the targets of diamide *** point mutations G4946E and I4790M(numbering according to Plutella xylostella,PxRyR)in the transmembrane domain of the insect RyRs associated with diamide resistance have so far been identified in three lepidopteran pests,***,Tuta absoluta and Chilo *** this study,we identified one of the known RyR target site resistance mutations(I4790M)in a field-collected population of Spodoptera *** field-collected WF population of *** exhibited 154 fold resistance to chlorantraniliprole when compared with the susceptible WH-S *** the transmembrane domains of *** RyR(SeRyR)revealed that the resistant WF strain was homozygous for the 14743M mutation(corresponding to I4790M in PxRyR),whereas the G4900E allele(corresponding to G4946E of PxRyR)was not *** 4743M allele was introgressed into the susceptible WH-S strain by crossing WF with WH-S,followed by three rounds of backcrossing with *** introgressed strain 4743M was homozygous for the mutant 4743M allele and shared about 94%of its genetic background with that of the recipient WH-S *** with WH-S,the near-isogenic 4743M strain showed moderate levels of resistance to chlorantraniliprole(21 fold),cyantraniliprole(25 fold)and flubendiamide(22 fold),suggesting that the I4743M mutation confers medium levels of resistance to all three *** analysis showed diamide resistance in the 4743M strain was inherited as an autosomal and recessive *** from this study have direct implications for the design of appropriate resistance monitoring and management practices to sustainably control ***.

关键词： beet armyworm diamide insecticides point mutation resistance ryanodine receptor

FDX1 as a novel biomarker and treatment target for stomach adenocarcinoma

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1803-1824页

作者： Xian-Ze Xie Lei zuo Wei Huang Qiao-Mei Fan ya-yun Weng Wen-Dong yao Jia-Li Jiang Jia-Qi Jin Department of Pharmacy The First Affiliated Hospital of Zhejiang Chinese Medical University(Zhejiang Provincial Hospital of Chinese Medicine)Hangzhou 310018Zhejiang ProvinceChina Anhui Province Huainan City Shou County Agricultural Machinery Affairs Management Center Huainan 232200Anhui ProvinceChina

BACKGROUND Stomach adenocarcinoma(STAD)is one of the main reasons for cancer-related deaths *** investigation aimed to define the connection between STAD and Cuproptosis-related genes(CRGs).Cuproptosis is a newly identified form of mitochondrial cell death triggered by *** To explore the identification of potential biomarkers for STAD disease based on *** A predictive model using Gene Ontology(GO),Least Absolute Shrinkage and Selection Operator(LASSO),Kyoto Encyclopedia of Genes and Genomes(KEGG),Gene Set Variation Analysis(GSVA),and Gene Set Enrichment Analysis analyzed gene interconnections,focusing on 3 copper-related genes and their expression in The Cancer Genome *** for mRNA-miRNA and mRNA-transcription factor interactions were *** prognostic significance of CRG scores was evaluated using time-receiver operating characteristic,Kaplan-Meier curves,and COX regression *** was conducted with datasets GSE26942,GSE54129,and *** of copper-related differ-entially expressed genes was also analyzed in various human tissues and gastric cancer subpopulations using the human protein *** Three significant genes(FDX1,LIAS,MTF1)were identified and selected via LASSO analysis to predict and classify individuals with STAD into high and low CRG score *** genes were down-regulated in both risk *** and KEGG analyses highlighted their involvement mainly in the electron transport *** validating their differential expression,FDX1 emerged as the most accurate diagnostic marker for gastric ***,the RCircos package localized FDX1 on chromosome *** Our study revealed that FDX1 could be a potential biomarker and treatment target for gastric malignancy,providing new ideas for further scientific research.

关键词： Stomach adenocarcinoma disease Copper death FDX1 prognostic biomarkers Cuproptosis-related genes

Surface-targeted functionalization of nickel-rich cathodes through synergistic slurry additive approach with multi-level impact using minimal quantity

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Nano Research 2024年第1期17卷 333-343页

作者： Jing Zhang Jiapei Li Longhao Cao Wenhua Cheng Ziyin Guo Xiuxia zuo Chao Wang ya-Jun Cheng Yonggao Xia Yudai Huang State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources College of ChemistryXinjiang UniversityUrumqi 830017China Ningbo Institute of Materials Technology&Engineering Chinese Academy of SciencesNingbo 315201China School of Materials Science and Engineering Tongji UniversityShanghai 200240China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

LiNi0.8Co0.1Mn0.1O_(2)(NCM811),a Ni-rich layered oxide,is a promising cathode material for high-energy density lithium-ion batteries(LIBs).However,its structural instability,caused by adverse phase transitions and continuous oxygen release,as well as deteriorated interfacial stability due to excessive electrolyte oxidative decomposition,limits its widespread *** address these issues,a new concept is proposed that surface targeted precise functionalization(STPF)of the NCM811 cathode using a synergistic slurry additive(SSA)*** approach involves coating the NCM811 particle surface with 3-aminopropyl dimethoxy methyl silane(3-ADMS),followed by the precise deposition of ascorbic acid via an acid-base *** slurry additives induce the formation of an ultra-thin spinel surface layer and a stable cathode–electrolyte interface(CEI),which enhances the electrochemical kinetics and inhibits crack *** STPF strategy implemented by the SSA approach significantly improves the cyclic stability and rate performance of the NCM811 cathode in both half-cell and full-cell *** work establishes a promising strategy to enhance the structural stability and electrochemical performance of nickel-rich cathodes and provides a feasible route to promote practical applications of high-energy density lithium-ion battery technology.

关键词： nickel-rich cathode slurry additive lithium-ion battery cathode–electrolyte interface ascorbic acid phase transition

Design and fabrication of lightweight AlCrFeNiTi_(x) compositionally complex alloys with exceptional specific strength

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Rare Metals 2024年第7期43卷 3314-3328页

作者： Jun-Zhi Li Wei-Zong Bao Jie Chen Bo-Hua Yu Kun zuo Tian Gao Ze-yun Cai Guo-Qiang Xie School of Materials Science and Engineering Harbin Institute of Technology(Shenzhen)Shenzhen 518055China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001China

This study focuses on compositionally complex alloys(CCAs),aiming to achieve a balance between high strength and low density for new energy and aerospace *** composition of AlCrFeNiTi_(x) CCAs is strategically guided by employing density functional theory and the theoretical design of thermodynamic *** CCAs,particularly AlCrFeNiTi_(0.25) alloy,demonstrate remarkable specific yield strength(1640.8 MPa) and 22.7% maximum *** incorporation of Ti facilitates the formation of lightweight and high-strength L2_(1)phase,contributing to the overall high specific *** effects of grain boundary strengthening,solid solution strengthening,Orowan strengthening and Peierls flow stress further enhance *** exploration of micros tructural changes during fracture reveals the role of ordered phases in suppressing crack propagation and absorbing energy within disordered phases,thereby improving the toughness and fracture resistance of *** methods and discoveries establish a robust foundation for advancing the development of novel lightweight CCAs.

关键词： Lightweight Compositionally complex alloys Specific yield strength

Intermediate phase-assisted growth of CsPbBr_(3) for high performance of carbon-based perovskite solar cells

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Rare Metals 2024年第5期43卷 2222-2229页

作者： Bo-yan Liu He-Ming yang Yu-Dong zuo Zhi-Hao Tao Jun-Chun Li ya-Jing Chang Guo-Qing Tong yang Jiang School of Materials Science and Engineering Hefei University of TechnologyHefei 230009China State Key Laboratory of Pulsed Power Laser Technology National University of Defense TechnologyHefei 230037China

CsPbBr_(3)inorganic perovskites have been regarded as the promising materials in the field of photo-voltaics because of the high tolerance against *** high energy barrier of phase transition from lead bromide(PbBr_(2))to CsPbBr_(3)perovskite and low solubility of perovskite in organic solvent impede the further improvement of device performance in terms of CsPbBr_(3)solar ***,an intermediate phase-assisted growth of CsPbBr_(3)perovskite was proposed by introducing tetraphenylphosphonium bromide(TPPB)as *** TPPB is expected to react with PbBr_(2)in organic solvent to form an intermediate phase of[***]-PbBr_(2),which not only effectively improves the crystallinity of PbBr2 crystals,but also greatly reduces the phase transition energy barrier,leading to uniform and compact CsPbBr_(3)perovskite films with large grain size and high *** combination with carbon electrode,the CsPbBr_(3)solar cells yield a champion device performance of 9.57%in comparison of pristine CsPbBr_(3)solar cells showing a low efficiency of 8.17%.Furthermore,the intermediate phaseassisted growth of CsPbBr_(3)-based solar cells displays an outstanding storage over720 h.

关键词： CsPbBr_(3) Perovskite solar cells Carbon electrode Hole transport layer free Intermediate phase

Repair tolerance assessment for aircraft composite structures using Bayesian updating

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Chinese Journal of Aeronautics 2024年第6期37卷 360-391页

作者： Xin LI Hongfu zuo Bin yaNG School of Automotive and Rail Transit Nanjing Institute of TechnologyNanjing 211167China Civil Aviation Key Laboratory of Aircraft Health Monitoring and Intelligent Maintenance College of Civil AviationNanjing University of Aeronautics and AstronauticsNanjing 211106China

Detection and repair of composite damage is crucial to ensure the safety and reliability of aircraft structures.A novel approach to quantitatively evaluate the repair tolerance of composite structures in civil aircraft based on Bayesian updating is *** method incorporates historical damage inspection data to determine the prior distribution of damage size,which is then updated with newly collected damage size data using Bayesian *** Carlo simulation is employed to investigate the probability of failure and estimate maintenance costs,considering various factors such as the frequency and timing of damage events,damage detection,structural strength,gust loads,and maintenance expenses throughout the lifecycle of composite *** and economic factors are considered to establish a lower threshold for repairs and an upper threshold for maintenance based on the occurrence of accidental impact *** of the effectiveness and feasibility of a quantitative assessment method for repair tolerance is conducted using damage statistics data from civil aircraft routes utilizing the structural skin panels of composite outer *** results demonstrate that the method proposed in conjunction with extensive simulations and full utilization of field damage inspection data can effectively simulate unexpected impact damage situations that may occur during civil aircraft service and evaluate the reliability and economic feasibility of the repair of *** research findings hold significant theoretical and practical value for the preparation of documents for continued airworthiness of composite structures,including structural repair manuals and maintenance programs.

关键词： Composite structures Repair tolerance Maintenance Bayesian updating Probability of failure

A new elastoplastic model for bolt-grouted fractured rock

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International Journal of Mining Science and Technology 2024年第7期34卷 995-1016页

作者： Haoyi Li Shuangying zuo Peiyuan Lin State Key Laboratory for Tunnel Engineering Sun Yat-Sen UniversityGuangzhou 510275China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519082China School of Civil Engineering Sun Yat-Sen UniversityGuangzhou 510275China College of Resources and Environmental Engineering Guizhou UniversityGuiyang 550025China

Complexities in mechanical behaviours of rock masses mainly stem from inherent discontinuities,which calls for advanced bolt-grouting techniques for stability *** the mechanical properties of bolt-grouted fractured rock mass(BGFR)and developing accurate prediction methods are crucial to optimize the BGFR support *** paper establishes a new elastoplastic(E-P)model based on the orthotropic and the Mohr-Coulomb(M-C)plastic-yielding *** elastic parameters of the model were derived through a meso-mechanical analysis of composite materials mechanics(CMM).Laboratory BGFR specimens were prepared and uniaxial compression test and variable-angle shear test considering different bolt arrangements were carried out to obtain the mechanical parameters of the *** showed that the anisotropy of BGFR mainly depends on the relative volume content of each component material in a certain ***,the mechanical parameters deduced from the theory of composite materials which consider the short fibre effect are shown to be in good agreement with those determined by laboratory experiments,and the variation rules maintained good ***,a case study of a real tunnel project is provided to highlight the effectiveness,validity and robustness of the developed E-P model in prediction of stresses and deformations.

关键词： Bolt-grouted fractured rock mass Elastoplastic model Composite materials mechanics Laboratory experiment

Motion-pressure coupled control and simulation of long-endurance capability for multicapsule stratospheric airships

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Chinese Journal of Aeronautics 2024年第6期37卷 137-150页

作者： Zhiguang SHI Wei HUO Zongyu zuo School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China Beijing Near Space Airship Technology Development Co. LtdBeijing 100070China

The current study focuses on the motion-pressure coupled control for a multicapsule stratospheric airship and transforms the path-tracking and heading-hold control of airships into guidance tracking with a time-varying weighted sum of longitudinal and lateral velocities by the definition of compound ***,an improved nonlinear predictive control method is provided to reduce the control energy consumption by the rolling optimization of controller parameters based on finite time intervals,ensuring infinite-time path-tracking ***,combined with the proposed cyclic regulation process of safe pressure between internal and external capsules,this study can fully reflect the force-thermal coupled rule of airships under the actions of atmospheric environment and maneuvering force,while evaluating the long-endurance capability of airships under the conditions of safe superheating and *** effectiveness of the motionpressure coupled controller was verified through numerical simulations,which can overcome the influence of environmental wind and achieve a tracking effect for the desired cruise path and compound *** airspeed provided during the cyclic circadian time caused the maximum superheating of the helium controlled within 30*** helium in the internal and external capsules achieved circadian *** equivalent micropore diameter of the capsule of 5 mm can achieve 55 days of long-endurance *** controller satisfies the requirements of cruise-flight application modes for multicapsule stratospheric airships with important engineering value.

关键词： Compound speed Nonlinear predictive control Cyclic pressure regulation Long-endurance capability Stratospheric airship