Identification of the ryanodine receptor mutation I4743M and its contribution to diamide insecticide resistance in Spodoptera exigua (Lepidoptera: Noctuidae)
到在 Spodoptera exigua 的 diamide 杀虫药剂电阻的 ryanodine 受体变化 I4743M 和它的贡献的鉴定(鳞翅目: Noctuidae )作者机构：College of Plant ProtectionNanjing Agricultural UniversityNanjingChina Bayer CropScienceR&D Pest ControlMonheimGermany
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2020年第27卷第4期
页 面：791-800页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
主 题：beet armyworm diamide insecticides point mutation resistance ryanodine receptor
摘 要：Insect ryanodine receptors(RyRs)are the targets of diamide *** point mutations G4946E and I4790M(numbering according to Plutella xylostella,PxRyR)in the transmembrane domain of the insect RyRs associated with diamide resistance have so far been identified in three lepidopteran pests,***,Tuta absoluta and Chilo *** this study,we identified one of the known RyR target site resistance mutations(I4790M)in a field-collected population of Spodoptera *** field-collected WF population of *** exhibited 154 fold resistance to chlorantraniliprole when compared with the susceptible WH-S *** the transmembrane domains of *** RyR(SeRyR)revealed that the resistant WF strain was homozygous for the 14743M mutation(corresponding to I4790M in PxRyR),whereas the G4900E allele(corresponding to G4946E of PxRyR)was not *** 4743M allele was introgressed into the susceptible WH-S strain by crossing WF with WH-S,followed by three rounds of backcrossing with *** introgressed strain 4743M was homozygous for the mutant 4743M allele and shared about 94%of its genetic background with that of the recipient WH-S *** with WH-S,the near-isogenic 4743M strain showed moderate levels of resistance to chlorantraniliprole(21 fold),cyantraniliprole(25 fold)and flubendiamide(22 fold),suggesting that the I4743M mutation confers medium levels of resistance to all three *** analysis showed diamide resistance in the 4743M strain was inherited as an autosomal and recessive *** from this study have direct implications for the design of appropriate resistance monitoring and management practices to sustainably control ***.
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