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Albumin fusion with granulocyte-macrophage colony-stimulating factor acts as an immunotherapy against chronic tuberculosis

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Cellular & Molecular Immunology 2021年第10期18卷 2393-2401页

作者： Yu-Min Chuang Liangmei He Michael L.Pinn ya-chea tsai Max A.Cheng Emily Farmer Petros C.Karakousis Chien-Fu Hung Department of Pathology Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Medicine Johns Hopkins University School of MedicineBaltimoreMDUSA Section of Infectious Diseases Department of Internal MedicineYale University School of MedicineNew HavenCTUSA

A long duration of treatment and emerging drug resistance pose significant challenges for global tuberculosis(TB)eradication ***,there is an urgent need to develop novel strategies to shorten TB treatment regimens and to treat drug-resistant *** an albumin-fusion strategy,we created a novel albumin-fused granulocyte-macrophage colony-stimulating factor(albGM-CSF)molecule that harnesses albumin’s long half-life and targeting abilities to enhance the biostability of GM-CSF and direct it to the lymph nodes,where the effects of GM-CSF can increase dendritic cell populations crucial for eliciting a potent immune *** this study,we demonstrate that albGM-CSF serves as a novel immunotherapy for chronic Mycobacterium tuberculosis(Mtb)infections by enhancing GM-CSF biostability in ***,albumin is very safe,stable,and has a long half-life,thereby enhancing the biostability of *** the lungs and draining lymph nodes,albGM-CSF is able to increase the numbers of dendritic cells,which are crucial for the activation of naive T cells and for eliciting potent immune *** administration of albGM-CSF alone reduced the mean lung bacillary burden in mice with chronic tuberculosis *** GM-CSF administration was associated with IL-1βrelease from Mtb-infected dendritic cells and macrophages,higher IL-1βlevels were observed in albGM-CSF-treated mice with chronic tuberculosis infection than in mice receiving *** fusion with GM-CSF represents a promising strategy for the control of chronic lung tuberculosis infections and serves as a novel therapeutic vaccination platform for other infectious diseases and malignancies.

关键词： Tuberculosis albumin GM-CSF therapeutic vaccine fusion protein

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Success of intravenous immunoglobulin and steroids in managing severe COVID-19 following lung transplantation:A case report

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World Journal of Transplantation 2025年第1期15卷 178-184页

作者： Yen-Shou Kuo Kuan-Hsun Lin Ying-Yi Chen Yuan-Ming tsai Ti-Hui Wu Hsu-Kai Huang tsai-Wang Huang Division of Thoracic Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical CenterTaipei 114202Taiwan

BACKGROUND Coronavirus disease 2019(COVID-19)pneumonia with severe septic shock and acute respiratory distress syndrome(ARDS)are critical illnesses for patients following *** immunoglobulin(IVIG)plays a role in both immune support and inflammation control,especially in immunocompromised *** case report describes the first successful experience using IVIG and pulse steroids to manage this critical condition following lung *** SUMMARY A 65-year-old male patient reported a history of chronic obstructive pulmonary disease and poor lung function and received bilateral sequential lung ***,he developed COVID-19 pneumonia,severe septic shock,and *** recovered from this critical condition after empirical antibiotics administration and veno-venous extracorporeal membrane oxygenation,in addition to IVIG and pulse *** IVIG is a valuable adjunct in managing severe sepsis in lung transplant recipients after COVID-19 *** aim,for the first time,to report the success of such a management approach for COVID-19 ARDS and sepsis in the post-lung transplant *** further investigations,this is a starting point for wider analysis of such an approach in this setting and consequently helps guide clinical practice for such a challenging patient population moving forward.

关键词： COVID-19 Septic shock Intravenous immunoglobulin Lung transplantation Post-transplant complications Case report

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Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture

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Cell Research 2005年第8期15卷 639-657页

作者： ya Huei CHEN,Yi Jung tsai,Fure Chyi CHEN Institute of Tropical Agriculture & International Cooperation National Pingtung University of Science & Technology Institute and Department of Plant Industry National Pingtung University of Science & Technology Pingtung 91201 Taiwan Institute of Tropical Agriculture & International Cooperation National Pingtung University of Science & Technology Pingtung 91201 Taiwan Pingtung 91201 Taiwan Institute and Department of Plant Industry National Pingtung University of Science & Technology Pingtung 91201 Taiwan Institute of Biotechnology National Pingtung University of Science & Technology Pingtung 91201 Taiwan

Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed.

关键词： Phalaenopsis tissue culture peloric mutant cDNA-RAPD suppression subtractive hybridization.

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User-guided line abstraction using coherence and structure analysis

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Computational Visual Media 2017年第2期3卷 177-188页

作者： Hui-Chi tsai ya-Hsuan Lee Ruen-Rone Lee Hung-Kuo Chu Department of Computer Science "National Tsing Hua University" Information and Communications Research Laboratories Industrial Technology Research Institute

Line drawing is a style of image abstraction where the perceptual content of the image is conveyed using distinct straight or curved lines. However,extracting semantically salient lines is not trivial and mastered only by skilled artists. While many parametric filters have successfully extracted accurate and coherent lines, their results are sensitive to parameter choice and easily lead to either an excessive or insufficient number of lines. In this work, we present an interactive system to generate concise line abstractions of arbitrary images via a few user specified strokes. Specifically, the user simply has to provide a few intuitive strokes on the input images, including tracing roughly along edges and scribbling on the region of interest, through a sketching interface. The system then automatically extracts lines that are long,coherent and share similar textural structures to form a corresponding highly detailed line drawing. We have tested our system with a wide variety of images. Our experimental results show that our system outperforms state-of-the-art techniques in terms of quality and efficiency.

关键词： line abstraction interactive drawing coherence strokes structure strokes stroke matching

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Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells

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International Journal of Ophthalmology(English edition) 2018年第2期11卷 189-195页

作者： ya-Yu Chen Chia-Fang tsai Ming-Chu tsai Wen-Kang Chen Yu-Wen Hsu Fung-Jou Lu Institute of Medicine College of MedicineChung Shan Medical UniversityTaichung City 40201TaiwanChina Department of Biotechnology TransWorld UniversityDouliu City 64063TaiwanChina Department of Applied Cosmetology Tainan Junior College of NursingTainan City 70043TaiwanChina Department of Optometry Da-Yeh UniversityChanghua 51591TaiwanChina Department of Ophthalmology Chung Shan Medical University HospitalTaichung City 40201TaiwanChina

AIM： To investigate the anti-fibrosis effect of rosmarinic acid（RA） in pterygium epithelial cells（PECs） to determine if RA is a potent agent for treating ***： The PECs（1×10-4 cells/mL） were treated with 100 μmol/L of RA for 1, 3 and 6h. After RA treatment, the cell viability was determined by staining with acridine orange/DAPI and analysis via a NucleoC ounter NC-3000. The protein expression levels of type I collagen, transforming growth factor beta-1（TGF-β1）, TGF-β type Ⅱ receptor（TGF-βRⅡ）, p-Smad1/5, p-Smad2, p-Smad3, and Smad4 of the cell lysates were measured by Western blot ***： The cell viability of PECs was significantly decreased after RA treatment（P〈0.01）. As the result, RA reduced the protein expression of typeⅠcollagen and TGF-β1 of PECs. Additionally, RA also inhibited TGF-β1/Smad signaling by decreasing the protein expressions of TGF-βRII, p-Smad1/5, p-Smad2, p-Smad3, and ***： This study demonstrate that RA could inhibit fibrosis of PECs by down-regulating type I collagen expression and TGF-β1/Smad signaling. Therefore, RA is a potent therapeutic agent for the treatment of pterygium.

关键词： fibrosis pterygium rosmarinic acid transforming growth factor beta-1 typeⅠcollagen

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Traumatic giant cell tumor of rib: A case report

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World Journal of Clinical Cases 2022年第24期10卷 8662-8666页

作者： Ying-Shian Chen Hon-We Kao Hsin-ya Huang tsai-Wang Huang Department of Surgery Taichung Armed Forces General HospitalTaichung 41168Taiwan Department of Surgery Tri-Service General HospitalNational Defense Medical CenterTaipei 11490Taiwan Department of Pathology Tungs’Taichung Metroharbor HospitalTaichung 43503Taiwan Department of Surgery Changhua Christian HospitalChanghua 50006Taiwan

BACKGROUND Giant cell tumor(GCT)of the anterior rib origin is extremely *** report the first case of trauma-induced GCT of the *** SUMMARY A 22-year-old female developed a mass over the right anterior chest wall with pain 3 mo after a falling injury with blunt trauma of the right chest *** computed tomography(CT)showed a tumor originating from the right 6th rib with bony destruction,and a CT-guided needle biopsy revealed a *** completely resected the tumor with chest wall and performed *** pathological diagnosis was GCT of the *** months after surgery,no signs of recurrence were *** GCT of the rib after trauma has not been *** history-taking and image evaluation are essential for the differential diagnosis of unusual chest wall tumors.

关键词： Chest wall tumor Giant cell tumor Trauma Rib Bone neoplasm Case report

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Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells

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World Journal of Gastroenterology 2006年第28期12卷 4478-4484页

作者： Jane C-J Chao Shih-Wen Chiang Ching-Chiung Wang ya-Hui tsai Ming-Shun Wu School of Nutrition and Health Sciences TaipJMedical University Taipei110 Taiwan China School of Pharmacy Taipei MedicalUniversity Taipei 110 Taiwan China Division of Internal Medicine Taipei Medical University-Wan Fang Hospital Taipei 116 Taiwan China

AIM： To investigate the effect of hot water-extracted Lydurn barbarum （LBE） and Rehrnannia glutinosa （RGE） on cell proliferation and apoptosis in rat and/or human hepatocellular carcinoma （HCC） cells. METHODS： Rat （H-4-Ⅱ-E） and human HCC （HA22T/ VGH） cell lines were incubated with various concentrations （0-10 g/L） of hot water-extracted LBE and RGE. After 6-24 h incubation, cell proliferation （n = 6） was measured by a colorimetric method. The apoptotic cells （n = 6） were detected by flow cytometry. The expression of p53 protein （n = 3） was determined by SDS-PAGE and Western blotting. RESULTS： Crude LBE （2-5 g/L） and RGE （2-10 g/L） dose-dependently inhibited proliferation of H-4-Ⅱ-E cells by 11% （P 〈 0.05） to 85% （P 〈 0.01） after 6-24 h treatment. Crude LBE at a dose of 5 g/L suppressed cell proliferation of H-4-Ⅱ-E cells more effectively than crude RGE after 6-24 h incubation （P 〈 0.01）. Crude LBE （2-10 g/L） and RGE （2-5 g/L） also dose-dependently inhibited proliferation of HA22T/VGH cells by 14%-43% （P 〈 0.01） after 24 h. Crude LBE at a dose of 10 g/L inhibited the proliferation of HA22T/VGH cells more effectively than crude RGE （56.8% ＋ 1.6% vs 70.3% ＋ 3.1% of control, P = 0.0003 〈 0.01）. The apoptotic cells significantly increased in H-4-Ⅱ-E cells after 24 h treatment with higher doses of crude LBE （2-5 g/L） and RGE （5-10 g/L）（P 〈 0.01）. The expression of p53 protein in H-4-Ⅱ-E cells was 119% and 143% of the control group compared with the LBE-treated （2, 5 g/L） groups, and 110% and 132% of the control group compared with the RGE -treated （5, 10 g/L） groups after 24 h. CONCLUSION： Hot water-extracted crude LBE （2-5 g/L） and RGE （5-10 g/L） inhibit proliferation and stimulate p53-mediated apoptosis in HCC cells.

关键词： Lycium barbarum extract Rehmannia glutinosa extract Proliferation Apoptosis Hepatocellular carcinoma

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A HYBRID MODEL OF KNOWLEDGE MANAGEMENT FOR NEW SERVICE DEVELOPMENT

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Journal of Systems Science and Systems Engineering 2016年第4期25卷 424-447页

作者： August tsai Department of Industrial Management College of Management Chien Hsin University of Science and Technology Taoyuan32097 Taiwan China

Although many theories regarding the implementation of knowledge management （KM） in organizations have been proposed and studied, most applications tend to stand alone without incorporating the business processes. Different categories of knowledge provide different benefits and how to integrate various categories of KM into a hybrid approach as an effective KM manner remains strategically important, and yet is still understudied. Therefore, in this paper a hybrid model that integrates principal KM applications for new service development （NSD） and the measurement of the resulting f＇mancial benefits have been developed. The proposed KM model incorporates newsgroups, knowledge forums, knowledge asset management and knowledge application processes as a hybrid means for sharing organizational knowledge along two axes, explicit vs. implicit and individual vs. collective. One of the largest management consulting companies in Taiwan, China, whose process model of NSD was standing alone with KM applications, was selected for the case study. A set of hybrid KM processes was developed to implement the proposed KM model, and it illustrates an application with greater financial benefits for integrating hybrid KM practices into the business process. Based on knowledge value added （KVA） validation, the proposed KM model provides a new operating system for sharing NSD knowledge within an organization. Through the case study by measuring the achieved financial results, the proposed KM model is found to provide an exclusive hybrid platform with an empirical process model to address innovative approaches and practical values of KM within an organization.

关键词： Knowledge management new service development (NSD) newsgroup knowledge forum knowledge asset knowledge application process knowledge value added (KVA)

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Comparisons of Subjective and Objective Measures of Free-Living Daily Physical Activity and Sedentary Behavior in College Students

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Journal of Science in Sport and Exercise 2021年第2期3卷 186-194页

作者： ya-Wen Hsu Chia-Chang Liu Yen-Jung Chang Yi-Ju tsai Wan-Chi tsai You Fu School of Community Health Sciences University of NevadaReno1664 N.Virginia StreetRenoNV 89557USA Department of Hospital and Health Care Administration Chia Nan University of Pharmacy and ScienceTainanTaiwanChina Department of Health Promotion and Health Education National Taiwan Normal UniversityTaipeiTaiwanChina Department of Physical Therapy College of MedicineNational Cheng Kung UniversityTainanTaiwanChina Department of Medical Laboratory Science and Biotechnology Kaohsiung Medical UniversityKaohsiungTaiwanChina

Purpose To compare physical activity and sedentary behavior between four commonly used subjective and objective meas-ures:the 7-day Physical Activity Recall (7DPAR),International Physical Activity Questionnaire (IPAQ),pedometer,and *** A total of 130 college students completed four measures for the same 7 *** composition was measured using a bioelectric impedance *** signed rank tests and Spearman correlations were performed to compare estimates between activity *** Spearman correlations between different activity measures were further examined separately for the higher and the lower body fat *** Compared with accelerometer-derived data,both the 7DPAR and the IPAQ overestimated light physical activity(P < 0.001) while underestimated sedentary behavior (P < 0.001).Across comparisons,the highest correlation was found between accelerometers and pedometers on steps/day (r =0.72,P < 0.001).The 7DPAR and the IPAQ were correlated with each other for all physical activity variables and sedentary behavior (r =0.37-0.45).There were low correlations (r=0.20-0.47) between the 7DPAR,the IPAQ,and accelerometers in sedentary behavior,light physical activity,and vigorous physical *** correlations between different activity modalities were observed among individuals with lower body fat(r=0.41-0.80) than among those with higher body fat (r=0.31-0.65).Conclusions The 7DPAR and the IPAQ yielded comparable estimation of moderate physical activity relative to *** were significant differences in sedentary time across activity *** compositions should be considered when comparing the estimates of activity levels between subjective and objective instruments.

关键词： Accelerometer Pedometer Self-report Body fat College student

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PESOI: Process Embedded Service-Oriented Architecture

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软件学报 2006年第6期17卷 1470-1484页

作者： Wei-Tek tsai Department of Computer Science and Engineering Arizona State University Tempe AZ 85281 USA

Service-Oriented Architecture (SOA) has drawn significant attention recently, and numerous architecture approaches have been proposed to represent SOA-based applications. The architecture of SOA-based applications is different from traditional software architecture, which is mainly static. The architecture of an SOA-based application is dynamic, i.e., the application can be composed at runtime using existing services, and thus the architecture is really determined at runtime, instead of design time. SOA applications have provided a new direction for software architecture study, where the architecture can be dynamically changed at runtime to meet the new application requirements. This paper proposes a Process-Embedded Service-Oriented Infrastructure to build SOA-based applications. This infrastructure embeds the entire software lifecycle management and service-oriented system engineering into the application developed on this infrastructure. Thus, the users can easily re-develop the applications during operation to meet the changing environments and requirements, through the supports provided by the embedded infrastructure.

关键词：服务导向处理服务导向体系软件体系结构分类

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