Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells

作     者：Ya-Yu Chen Chia-Fang Tsai Ming-Chu Tsai Wen-Kang Chen Yu-Wen Hsu Fung-Jou Lu 

作者机构：Institute of MedicineCollege of MedicineChung Shan Medical UniversityTaichung City 40201TaiwanChina Department of BiotechnologyTransWorld UniversityDouliu City 64063TaiwanChina Department of Applied CosmetologyTainan Junior College of NursingTainan City 70043TaiwanChina Department of OptometryDa-Yeh UniversityChanghua 51591TaiwanChina Department of OphthalmologyChung Shan Medical University HospitalTaichung City 40201TaiwanChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2018年第11卷第2期

页      面：189-195页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by Ministry of Science and Technology Taiwan(No.NSC 106-2314-B-212-004) 

主　　题：fibrosis pterygium rosmarinic acid transforming growth factor beta 1 typeⅠcollagen 

摘      要：AIM： To investigate the anti-fibrosis effect of rosmarinic acid（RA） in pterygium epithelial cells（PECs） to determine if RA is a potent agent for treating ***： The PECs（1×10-4 cells/mL） were treated with 100 μmol/L of RA for 1, 3 and 6h. After RA treatment, the cell viability was determined by staining with acridine orange/DAPI and analysis via a NucleoC ounter NC-3000. The protein expression levels of type I collagen, transforming growth factor beta-1（TGF-β1）, TGF-β type Ⅱ receptor（TGF-βRⅡ）, p-Smad1/5, p-Smad2, p-Smad3, and Smad4 of the cell lysates were measured by Western blot ***： The cell viability of PECs was significantly decreased after RA treatment（P〈0.01）. As the result, RA reduced the protein expression of typeⅠcollagen and TGF-β1 of PECs. Additionally, RA also inhibited TGF-β1/Smad signaling by decreasing the protein expressions of TGF-βRII, p-Smad1/5, p-Smad2, p-Smad3, and ***： This study demonstrate that RA could inhibit fibrosis of PECs by down-regulating type I collagen expression and TGF-β1/Smad signaling. Therefore, RA is a potent therapeutic agent for the treatment of pterygium.