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氨基羧酸类配体强化Fe^(2+)活化分子氧降解对硝基酚

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中国环境科学 2024年第10期44卷 5630-5638页

作者：辛思怡张成武王瑛琪王德玉赵薇秦传玉任黎明吉林大学地下水资源与环境教育部重点实验室吉林长春130021 吉林大学石油化工污染场地控制与修复技术国家地方联合工程实验室吉林长春130021 吉林大学吉林省水资源与环境重点实验室吉林长春130021 中石化石油化工科学研究院有限公司北京100083

本研究构建了Fe^(2+)/O_(2)/乙二胺四乙酸钠(EDTA)、Fe^(2+)/O_(2)/次氮基三乙酸钠(NTA)、Fe^(2+)/O_(2)/乙二胺二琥珀酸(EDDS)高级氧化体系,选取对硝基酚(PNP)为目标污染物,探究3种体系降解PNP的性能,着重分析Fe^(2+)/O_(2)/EDTA、Fe^(2+)/O_(2)/NTA体系的ROS产生过程及对污染物的降解贡献,阐明氨基羧酸类配体的强化效果及机制.结果表明,Fe^(2+)/O_(2)/EDTA、Fe^(2+)/O_(2)/NTA体系的最佳降解条件均为pH=3、Fe^(2+)与配体物质的量比为1:1,PNP降解率分别为50.8%、81.2%.Fe^(2+)/O_(2)/EDTA体系生成的ROS为HO·与O_(2)^(·−),ROS产生途径存在单电子转移(O_(2)→O_(2)^(·−)→H_(2)O_(2)→HO·)、双电子转移(O_(2)→H_(2)O_(2)→HO·)途径,部分PNP被O_(2)^(·−)还原为对氨基酚,大多数PNP及其还原产物被HO·氧化生成对苯二酚和对苯醌,之后氧化开环;Fe^(2+)/O_(2)/NTA体系生成的ROS主要是HO·,体系ROS产生途径主要为双电子转移途径,PNP被HO·直接氧化,生成对苯二酚和对苯醌,之后氧化开环.此外探究了两种配体活化能力的差异及机制.

关键词：分子氧活化活性自由基乙二胺四乙酸钠次氮基三乙酸钠二价铁对硝基酚

Improving anode performances of lithium-ion capacitors employing carbon-Si composites

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Rare Metals 2019年第12期38卷 1113-1123页

作者： ya-Bin An Si Chen Min-Min Zou Lin-Bin Geng Xian-Zhong Sun Xiong Zhang Kai Wang yan-wei Ma Institute of Electrical Engineering Chinese Academy of SciencesBeijing 100190China College of Materials Science and Engineering Beijing Institute of Petrochemical TechnologyBeijing 102617China University of Chinese Academy of Sciences Beijing 100049China

The lithium-ion capacitor is a promising energy storage system with a higher energy density than traditional ***,its cycling and rate performances,which depend on the electrochemical properties of the anode,are still required to be *** this work,soft carbon anodes reinforced using carbon-Si composites of various compositions were fabricated to investigate their beneficial influences on the performance of lithium-ion *** results showed that the specific capacities of the anodes increased significantly by 16.6 mAh g^(-1) with 1.0 wt% carbon-Si composite,while the initial discharge efficiency barely *** specific capacity of the anode with a 10.0 wt% carbon-Si composite reached 513.1 mAh g^(-1),and the initial discharge efficiency was 83.79%.Furthermore,the anodes with 7.5 wt% or lower amounts of carbon-Si composite demonstrated reduced charge transfer resistances,which caused an improvement in the rate performance of the lithium-ion ***,the use of the optimized amount(7.5 wt%) of carbon-Si composite in the anode could significantly improve the cycling performance of the lithium-ion capacitor by compensating the consumption of active *** capacity retention of the lithium-ion capacitor reached 95.14% at 20 C after 10,000 cycles,while the anode potential remained below 0.412 V,which is much lower than that of a soft carbon anode.

关键词： Lithium-ion capacitor Soft carbon Carbon-Si composite Cycling performance Rate performance

Effect of Guizhi Fuling Capsule on Apoptosis of Myeloma Cells Through Mitochondrial Apoptosis Pathway

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Chinese Journal of Integrative Medicine 2023年第2期29卷 127-136页

作者： SUN Run-jie XU Jie GAO wei ZHANG yan-yu SUN Xiao-qi JI Lin CUI Xing Department of Traditional Chinese Medicine Shandong University of Traditional Chinese MedicineJinan 250014China Department of Hematology Affiliated Hospital of Shandong University of Traditional Chinese MedicineJinan 250014China Department of Laboratory Affiliated Hospital of Shandong University of Traditional Chinese MedicineJinan 250014China Department of Neurology the Second Affliated Hospital of Shandong University of Traditional Chinese MedicineJinan 250001China Department of Oncology the Second Affiliated Hospital of Shandong University of Traditional Chinese MedicineJinan 250001China

Objective: To observe the effects of Guizhi Fuling Capsule(GZFLC) on myeloma cells and explore the mechanisms. Methods: MM1S and RPMI 8226 cells were co-cultured with different concentrations of serum and the cell experiments were divided into negative(10%, 20% and 40%) groups, GZFLC(10%, 20%, and 40%)groups and a control group. Cell counting kit-8(CCK-8) assays and flow cytometry were used to detect the viability and apoptosis levels of myeloma cells. The effects on mitochondria were examined by reactive oxygen specie(ROS) and tetrechloro-tetraethylbenzimidazol carbocyanine iodide(JC-1) assays. Western blot was used to detect the expression of B cell lymphoma-2(Bcl-2), Bcl-2-associated X(Bax), cleaved caspase-3,-9, cytochrome C(Cytc)and apoptotic protease-activating factor 1(Apaf-1). RPMI 8226 cells(2×107) were subcutaneously inoculated into 48 nude mice to study the in vivo antitumor effects of GZFLC. The mice were randomly divided into four groups using a completely randomized design, the high-, medium-, or low-dose GZFLC(840, 420, or 210 mg/kg per day,respectively) or an equal volume of distilled water, administered daily for 15 days. The tumor volume changes in and survival times of the mice in the GZFLC-administered groups and a control group were observed. Cytc and Apaf-1 expression levels were detected by immunohistochemistry. Results: GZFLC drug serum decreased the viability and increased the apoptosis of myeloam cells(P<0.05). In addition, this drug increased the ROS levels and decreased the mitochondrial membrane potential(P<0.01). Western blot showed that the Bcl-2/Bax ratios were decreased in the GZFLC drug serum-treated groups, whereas the expression levels of cleaved caspase-3,-9,Cytc and Apaf-1 were increased(all P<0.01). Over time, the myeloma tumor volumes of the mice in the GZFLCadministered groups decreased, and survival time of the mice in the GZFLC-administered groups were longer than that of the mice in the control group. Immunohistochemical a

关键词： Guizhi Fuling Capsule mitochondrial apoptosis multiple myeloma

基于WebGIS的绿地资源信息化系统设计与实现

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计算机技术与发展 2024年第7期34卷 214-220页

作者：王亚波蒲智魏建新鲁文蕊任军新疆农业大学计算机与信息工程学院新疆乌鲁木齐830052 新疆激光雷达应用工程技术研究中心新疆乌鲁木齐830002 新疆自然资源信息中心新疆乌鲁木齐830002

基于WebGIS技术、数据库技术构建B/S架构的绿地资源信息化系统,旨在解决传统绿地资源信息数据管理方式可视化效果差、缺乏空间管理和数据不集中以及共享性差等问题。该系统以国土变更调查数据为基础,对乌鲁木齐市近十年绿地资源数据进... 详细信息

基于WebGIS技术、数据库技术构建B/S架构的绿地资源信息化系统,旨在解决传统绿地资源信息数据管理方式可视化效果差、缺乏空间管理和数据不集中以及共享性差等问题。该系统以国土变更调查数据为基础,对乌鲁木齐市近十年绿地资源数据进行分析和整合,建立了乌鲁木齐市绿地资源基础地理信息数据库,实现了自然资源管理新形势下的绿地数据共享和研究区绿地资源数据的集中管理。采用前后端分离开发,实现高内聚低耦合,增强系统的可维护性并综合考虑系统的安全性和数据访问机制,设计和实现了数据信息化、数据查询、统计分析、指标趋势分析、辅助审核和权限管理等功能。最终通过实际数据验证,较好地实现了系统的多项功能。系统不仅为绿地资源的研究提供了便捷的信息获取途径,同时在推进乌鲁木齐市绿地合理开发利用和绿地管理水平提升方面发挥了重要作用。此外,该系统的开发也为其他城市和地区的国土资源信息化建设提供了实际的技术参考。

关键词：绿地资源信息化管理空间分析 WebGIS PostgreSQL

不同施肥量与益生菌对掌叶大黄生长和土壤微生物组成的影响

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中国现代中药 2024年第6期26卷 1024-1035页

作者：周双双蔡瑜林亚石卓蓉张庆伟西南大学生命科学学院重庆400715 重庆灏天生态农业科技有限公司重庆408407 重庆市南川区道地中药材研究院重庆408407

目的:分析化肥和益生菌对掌叶大黄根茎初生和次生代谢、药用成分含量和土壤微生物群落组成的影响,为合理施肥提供参考。方法:对掌叶大黄幼苗进行2个施肥量(适量施肥、过量施肥)和添加3种益生菌(枯草芽孢杆菌、哈茨木霉菌、解淀粉芽孢杆... 详细信息

目的:分析化肥和益生菌对掌叶大黄根茎初生和次生代谢、药用成分含量和土壤微生物群落组成的影响,为合理施肥提供参考。方法:对掌叶大黄幼苗进行2个施肥量(适量施肥、过量施肥)和添加3种益生菌(枯草芽孢杆菌、哈茨木霉菌、解淀粉芽孢杆菌)处理;通过非靶向代谢组学分析不同处理对掌叶大黄根茎初生和次生代谢的影响;采用高效液相色谱法分析不同处理掌叶大黄根茎蒽醌含量的差异;通过16S rRNA测序和内转录间隔区测序分析不同处理掌叶大黄根际土壤微生物群落组成和多样性。结果:适量施肥和过量施肥均能显著增加掌叶大黄叶片和根茎的质量。在适量施肥的基础上添加益生菌处理,哈茨木霉菌和解淀粉芽孢杆菌对掌叶大黄根茎生长的促进作用优于枯草芽孢杆菌。在物质代谢方面,适量施肥和过量施肥均导致掌叶大黄根茎氨基酸水平升高,但过量施肥导致掌叶大黄根茎糖类和脂类水平降低。与适量施肥比较,哈茨木霉菌能够显著提高掌叶大黄根茎糖类、氨基酸和脂类水平,而解淀粉芽孢杆菌则导致糖类和氨基酸水平降低。在药用成分方面,过量施肥和解淀粉芽孢杆菌处理蒽醌含量明显偏低,适量施肥、枯草芽孢杆菌和哈茨木霉菌处理蒽醌含量较高。施肥对细菌群落组成影响较小,对真菌群落组成影响较大,施肥会导致土壤真菌中多种潜在病原菌(如楔孢黑粉菌属)丰度增加而使真菌整体丰度和多样性降低,益生菌处理(如枯草芽孢杆菌)能减轻施肥带来的影响。结论:综合掌叶大黄生长和药用成分含量情况,在多种施肥组合中,哈茨木霉菌能够较好地促进掌叶大黄根茎生长,并使其具有良好的营养状况和较高的药用成分含量。在保持或改善掌叶大黄种植土壤微生物群落多样性方面,枯草芽孢杆菌具有较好的效果。

关键词：掌叶大黄施肥方案益生菌次生代谢根际微生物 α多样性

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15吨蠕墨铸铁件工艺浅析

铸造设备与工艺 2024年第4期 11-15页

作者：傅强吕清轲许景峰梁亚茹戚鹏超潍柴重机股份有限公司山东潍坊262208

近年来,生产高牌号灰铸铁大型船用发动机机体多次出现裂纹缺陷,影响发动机的整体性能和使用寿命,鉴于蠕墨铸铁的特殊性能,开发大型船用蠕墨铸铁机体成为必然趋势。从蠕化处理、熔炼工艺和铸件检测三个方面优化和试验验证,逐一解决大吨... 详细信息

近年来,生产高牌号灰铸铁大型船用发动机机体多次出现裂纹缺陷,影响发动机的整体性能和使用寿命,鉴于蠕墨铸铁的特殊性能,开发大型船用蠕墨铸铁机体成为必然趋势。从蠕化处理、熔炼工艺和铸件检测三个方面优化和试验验证,逐一解决大吨位蠕墨铸铁生产技术难点,有效降低裂纹缺陷风险,最终实现对大型船用蠕墨铸铁件的批量稳定生产。

关键词：蠕墨铸铁喂线工艺熔炼工艺

Effects of methamidophos and deltamethrin on in vitro protein phosphorylation in Monochamus alternatus

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Insect Science 2008年第6期15卷 529-535页

作者： Jie Liu Xi-Wu Gao Yi-Jun Wu wei Li qi-Lian qin Jiang-Hua Sun State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing College of Agronomy and Biotechnology China Agricultural University Beijing China

Monochamus alternatus Hope （Coleoptera： Cerambycidae） is not only a serious pest insect to pine trees but also the main vector of pine wood nemadote Bursaphelenchus xylophilus, which causes pine wilt disease. To explore the insecticidal mechanism of insecticides to M. alternatus, we chose methamidophos and deltamethrin as the representatives of two groups of insecticides （organophosphates and pyrethroids）, which are widely used for pest control in China and investigated their effects on phosphorylation of proteins from the insect. Phosphorylation of proteins from the insect fat body and head was determined by in vitro 32P-labelling. In the fat body, deltamethrin obviously reduced basal phosphorylation levels of proteins at 111, 95, 77, and 44 kDa, but enhanced the basal phosphorylation level of a protein at 138 kDa. However, in the presence of calmodulin but not cyclic adenosine monophosphate （cAMP）, deltamethrin increased phosphorylation of the protein at 111 kDa. In the head, deltamethrin inhibited basal phosphorylation levels of proteins at 113, 98, and 51 kDa, but potentiated phosphorylation of a protein at 167 kDa activated by cAMP. Methamidophos inhibited phosphorylation of a protein at 44 kDa in the fat body. Although methamidophos did not impact basal phosphorylation levels of any proteins in the head, it inhibited calcium/calmodulin （Ca^2＋CaM）-stimulated phosphorylation of a protein at 51 kDa. Together, our data indicate that methamidophos and deltamethrin altered phosphorylation levels of various proteins in the head and fat body of the pine insect and these two kinds of insecticides acted on the proteins that can be phosphorylated in the tissues respectively, which is possibly related to their toxicity.

关键词： methamidophos deltamethrin Monochamus alternatus protein phosphorylation

SIRT1 facilitates amyloid beta peptide degradation by upregulating lysosome number in primary astrocytes

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Neural Regeneration Research 2018年第11期13卷 2005-2013页

作者： Min-Zhe Li Liang-Jun Zheng Jian Shen Xin-ya Li qi Zhang Xue Bai qing-Song Wang Jian-Guo li General Surgery Department Beijing Chao-Yang HospitalCapital Medical University Beijing China State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing China

Previous studies have shown that sirtuin 1（SIRT1） reduces the production of neuronal amyloid beta（Aβ） and inhibits the inflammatory response of glial cells, thereby generating a neuroprotective effect against Aβ neurotoxicity in animal models of Alzheimer＇s disease. However, the protective effect of SIRT1 on astrocytes is still under investigation. This study established a time point model for the clearance of Aβ in primary astrocytes. Results showed that 12 hours of culture was sufficient for endocytosis of oligomeric Aβ, and 36 hours sufficient for effective degradation. Immunofluorescence demonstrated that Aβ degradation in primary astrocytes relies on lysosome function. Enzymatic agonists or SIRT1 inhibitors were used to stimulate cells over a concentration gradient. Aβ was co-cultured for 36 hours in medium. Western blot assay results under different conditions revealed that SIRT1 relies on its deacetylase activity to promote intracellular Aβ degradation. The experiment further screened SIRT1 using quantitative proteomics to investigate downstream, differentially expressed proteins in the Aβ degradation pathway and selected the ones related to enzyme activity of SIRT1. Most of the differentially expressed proteins detected are close to the primary astrocyte lysosomal pathway. Immunofluorescence staining demonstrated that SIRT1 relies on its deacetylase activity to upregulate lysosome number in primary astrocytes. Taken together, these findings confirm that SIRT1 relies on its deacetylase activity to upregulate lysosome number, thereby facilitating oligomeric Aβ degradation in primary astrocytes.

关键词： nerve regeneration amyloid beta peptide Alzheimer's disease neurodegeneration astrocytes gliocytes sirtuin 1 quantitative proteomics lysosome time point model peptide degradation neural regeneration

Association between cadherin-17 expression and pathological characteristics of gastric cancer:A meta-analysis

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World Journal of Gastroenterology 2015年第12期21卷 3694-3705页

作者： Zi-Wen Long Meng-Long Zhou Jing-wei Fu Xian-Qun Chu ya-Nong Wang Department of Gastric Cancer and Soft-tissue Sarcoma Surgery Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University

AIM: To construct a meta-analysis in order to examine the relationship between cadherin-17(CDH17) andgastric cancer(GC). METHODS: Related articles were selected by searching the following English or Chinese electronic databases: CINAHL, MEDLINE, Science Citation Index, the Chinese Journal Full-Text, and the weipu Journal. NewcastleOttawa Scale(NOS) criteria were used to ensure consistency in reviewing and reporting results. Statistical analyses were conducted with Version 12.0 STATA statistical ***: Ultimately, 11 articles, with a total of 2,120 GC patients, were found to be eligible for study inclusion. In comparisons of GC patients by TNM stage(Ⅲ-Ⅳ vs ?Ⅰ-Ⅱ: OR = 2.35, 95%CI: 1.15-4.825, P = 0.019), histologic grade(3-4 vs 1-2: OR = 3.48, 95%CI: 1.36-8.92, P = 0.009), invasion grade(T3-4 vs T1-2: OR = 2.86; 95%CI: 1.69-4.83; P = 0.000), and lymph node metastasis(positive vs negative: OR = 2.64; 95%CI: 1.33-5.27; P = 0.006), it was found that CDH17 showed more positive expressions in each of the more severe cases. Country-stratified analyses from all four experimental subgroups showed that high CDH17 expression levels may be related to GC among Chinese and Korean populations(all P < 0.05), with the exception of the invasion grade T3-4 vs T1-2 comparison, where the relation only held among the Chinese population(OR = 2.86, 95%CI: 1.69-4.83, P = 0.000). CONCLUSION: Collectively, the data reflects the capacity of CDH17 in tumor proliferation and metastasis among GC patients.

关键词： Cadherin 17 Protein expression Gastric cancer Meta