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Apoptosis in oncology

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Cell Research 2001年第1期11卷 1-7页

作者： FAN XIAO QIANG, ya jun guo (International Joint Cancer Institute, The Second Military Medical University, Shanghai 200423, China) 1International Joint Cancer Institute The Second Military Medical University Shanghai 200433 China 1International Joint Cancer Institute The Second Military Medical University Shanghai 200433 China

Apoptosis is a complex process involving a large array of genes and mutation of any of these genes may lead to malignancy formation. Re-acquirement of FasL by tumor cells may enable them to evade the surveillance of immune system and thus contributes to the growth of tumor. Apart from traditional therapies, inducing apoptosis of tumor cell by new methods employing death receptor ligands and making use of Fas counterattack is also being developed.

关键词： Apoptosis mitochondria death receptor death receptor ligand tumor therapy.

Improved discriminative training for generative model

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The Journal of China Universities of Posts and Telecommunications 2009年第3期16卷 126-130页

作者： WU ya-hui,guo jun,LIU Gang Laboratory of Pattern Recognition and Intelligent System,Beijing University of Posts and Telecommunications,Beijing 100876,China [a]Laboratory of Pattern Recognition and Intelligent System Beijing University of Posts and Telecommunications Beijing 100876 China

This article proposes a model combination method to enhance the discriminability of the generative model. Generative and discriminative models have different optimization objectives and have their own advantages and drawbacks. The method proposed in this article intends to strike a balance between the two models mentioned above. It extracts the discriminative parameter from the generative model and generates a new model based on a multi-model combination. The weight for combining is determined by the ratio of the inter-variance to the intra-variance of the classes. The higher the ratio is, the greater the weight is, and the more discriminative the model will be. Experiments on speech recognition demonstrate that the performance of the new model outperforms the model trained with the traditional generative method.

关键词： model combination discriminability generative model training speech recognition

Plutella xylostella granulovirus late gene promoter activity in the context of the Autographa californica multiple nucleopolyhedrovirus genome

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Virologica Sinica 2016年第3期31卷 229-239页

作者： He-Lin Ren Yuan Hu ya-jun guo Lu-Lin Li Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life SciencesCentral China Normal University

Within Baculoviridae, little is known about the molecular mechanisms of replication in betabaculoviruses, despite extensive studies in alphabaculoviruses. In this study, the promoters of nine late genes of the betabaculovirus Plutella xylostella granulovirus(Plxy GV) were cloned into a transient expression vector and the alphabaculovirus Autographa californica multiple nucleopolyhedrovirus(Ac MNPV) genome, and compared with homologous late gene promoters of Ac MNPV in Sf9 cells. In transient expression assays, all Plxy GV late promoters were activated in cells transfected with the individual reporter plasmids together with an Ac MNPV bacmid. In infected cells, reporter gene expression levels with the promoters of Plxy GV e18 and Ac MNPV vp39 and gp41 were significantly higher than those of the corresponding Ac MNPV or Plxy GV promoters,which had fewer late promoter motifs. Observed expression levels were lower for the Plxy GV p6.9,pk1, gran, p10 a, and p10 b promoters than for the corresponding Ac MNPV promoters, despite equal numbers of late promoter motifs, indicating that species-specific elements contained in some late promoters were favored by the native viral RNA polymerases for optimal transcription. The 8-nt sequence TAAATAAG encompassing the ATAAG motif was conserved in the Ac MNPV polh, p10,and pk1 promoters. The 5-nt sequence CAATT located 4 or 5 nt upstream of the T/ATAAG motif was conserved in the promoters of Plxy GV gran, p10 c, and pk1. The results of this study demonstrated that Plxy GV late gene promoters could be effectively activated by the RNA polymerase from Ac MNPV, implying that late gene expression systems are regulated by similar mechanisms in alphabaculoviruses and betabaculoviruses.

关键词： Plutella xylostella granulovirus(PlxyGV) Autographa californica multiple nucleopolyhedrovirus(AcMNPV) late gene expression

Preparation and catalytic properties of mesoporous nV ‐MCM‐41 for propane oxidative dehydrogenation in the presence of CO_2

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Chinese Journal of Catalysis 2018年第6期39卷 1099-1109页

作者： Zai‐Fang Han Xu‐Liang Xue Jian‐Min Wu Wan‐Zhong Lang ya‐jun guo The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials Department of Chemistry and Chemical Engineering Shanghai Normal University Shanghai 200234 China

The nV‐MCM‐41 catalysts were prepared by one‐step hydrothermal synthesis and applied to the oxidative dehydrogenation of propane（ODHP） in the presence of CO2. Several state‐of‐the‐art char‐acterization methods were performed to explore the correlation between catalytic performance and the physicochemical characterizations of the catalysts. Because moderate amounts of V species were introduced into the framework of MCM‐41, the catalyst maintained a large specific surface area, a highly ordered mesoporous structure, and highly dispersed V active sites（monomeric and dimeric V oxide species）, while the high‐vanadium‐doping catalysts caused an enhancement in the number of acidic sites and V2O5 crystallites. The ODHP reaction showed that the 6.8 V‐MCM‐41（V content 6.8 wt%） catalyst exhibits high activity and stability, and the C3H8/CO2 molar ratio（1：4） was suitable. The promoting effect of CO2 on the oxidative dehydrogenation of ODHP was demonstrated as the reaction coupling mechanism and ＂lattice oxygen＂ mechanism.

关键词： Propane dehydrogenation Vanadium oxide MCM‐41 Mesoporous support Heterogeneous catalysis

Vena cava thrombosis after vena cava filter placement： Incidence and risk factors

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Journal of Geriatric Cardiology 2011年第2期8卷 99-103页

作者： ya-Juan guo jun Feng Tian-Rong Qu yan Qu ya-Min Liu Yu-Shun Zhang Hong-yan Tian Ai-Qun Ma Department of Cardiology and Periphery Vascular Medicine the First Affiliated Hospital of Medical College of Xi＇an Jiaotong University Xi＇an 710061 Shaanxi Province China

Background The objective of this study was to assess the clinical safety and efficacy of vena cava filter （VCF） placement, with particular emphasis on the incidence and risk factors of inferior vena cava thrombosis （VCT） after VCF placement. Methods Clinical data of patients with venous thromboembolism （VTE）, with or without placement of VCF, were analyzed in a retrospective single-center audit of medical records from January 2005 to june 2009. The collected data included demographics, procedural details, filter type, indications, and complications. Results A total of 168 cases of VTE （82 with VCF; 86 without VCF） were examined. Over a median follow-up of 24.2 months, VCT occurred in 18 of 82 patients with VCFs （11 males, 7 females, mean age 55.4 years）. In 86 patients without VCFs, VCT occurred in only 6 individuals （4 males, 2 females） during the study period. VCT was observed more frequently in patients fitted with VCFs than in those without VCFs （22% vs. 7.0%）. Conclusions The incidence of VCT in patients with VTE after VCF implantation was 22% approximately. Anticoagulation therapy should be continued for all patients with VCF placement, unless there is a specific contraindication. Almost all instances of VCT in patients with VCF implants in our study occurred after stopping anticoagulation treatment. The use of VCFs is increasing, and more trials are needed to confirm their benefit and accurately assess their safety.

关键词： vena cava filters venous thromboembolism complication

A human-specific insertion promotes cell proliferation and migration by enhancing TBC1D8B expression

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Science China(Life Sciences) 2024年第4期67卷 765-777页

作者： Hui Zhao Lin-Lin Liu Jian Sun Lian Jin Hai-Bing Xie Jian-Bo Li Hui Xu Dong-Dong Wu Xiao-Lin Zhuang Min-Sheng Peng ya-jun guo Wei-Zhu Qian Newton OOtecko Wei-Jie Sun Liang-Hu Qu Jie He Zhao-Li Chen Rong Liu Ce-Shi Chen ya-Ping Zhang State Key Laboratory for Conservation and Utilization of Bio-resource School of Life SciencesSchool of Ecology and Environmental ScienceYunnan UniversityKunming 650091China School of Forensic Medicine Kunming Medical UniversityKunming 650500China Academy of Biomedical Engineering Kunming Medical UniversityKunming 650500China The Third Affiliated Hospital Kunming Medical UniversityKunming 650118China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming 650201China State Key Laboratory of Genetic Resources and Evolution Yunnan Laboratory of Molecular Biology of Domestic AnimalsKunming Institute of ZoologyChinese Academy of SciencesKunming 650201China The Key Laboratory of Gene Engineering of the Ministry of Education State Key Laboratory for BiocontrolSun Yat-sen UniversityGuangzhou 510275China National Engineering Research Center for Antibody Medicine and Shanghai Key Laboratory of Cell Engineering and Antibody Shanghai 201203China Department of Thoracic Surgery Cancer Institute and HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Human-specific insertions play important roles in human phenotypes and *** we reported a 446-bp insertion(Insert-446)in intron 11 of the TBC1D8B gene,located on chromosome X,and traced its origin to a portion of intron 6 of the EBF1 gene on chromosome ***,Insert-446 was present in the human Neanderthal and Denisovans genomes,and was fixed in humans after human-chimpanzee *** have demonstrated that Insert-446 acts as an enhancer through binding transcript factors that promotes a higher expression of human TBC1D8B gene as compared with orthologs in *** addition,over-expression TBC1D8B promoted cell proliferation and migration through“a dual finger”catalytic mechanism(Arg538 and Gln573)in the TBC domain in vitro and knockdown of TBC1D8B attenuated tumorigenesis in *** of Insert-446 prevented cell proliferation and migration in cancer and normal *** results reveal that the human-specific Insert-446 promotes cell proliferation and migration by upregulating the expression of TBC1D8B *** findings provide a significant insight into the effects of human-specific insertions on evolution.

关键词： human-specific insertion enhancer gene expression cell proliferation and migration

Lentivirual vector-mediated doxycycline-inducible iASPP gene targeted RNA interference in hepatocellular carcinoma

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Chinese Journal of Cancer 2010年第9期29卷 796-801页

作者： Ming-Shu Pang Xia Chen Bin LU Jian Zhao Bo-Hua Li Yu-Quan Wei ya-jun guo State Key Laboratory of Biotherapy and Cancer Center West China Hospital West China Medical School Sichuan University Chengdu Sichuan 610041 P. R. China International Joint Cancer Institute the Second Military Medical University Shanghai 200433 P. R. China PLA General Hospital Cancer Center Chinese PLA Postgraduate Medical School Beijing 100853 P. R. China National Engineering Research Center of Antibody Medicine Shanghai 201203 P. R. China

Background and Objective:iASPP, an inhibitory member of the apoptosis-stimulating proteins of p53 (ASPP) family, has been found to be up-regulated in various human tumor *** study was to construct an efficient doxycycline-regulated, lentiviral vector-mediated knockdown system for iASPP that will allow for inducible down-regulation of iASPP gene expression and preliminary functional ***:A pair of complementary oligos with hairpin structures targeting the iASPP gene and a negative control were synthesized, then ligated with pLVTHM vector and *** fragment containing the shRNA cassette was cloned to pLVCT-tTR-KRAB *** recombinant vectors were co-transfected with viral packaging mix into 293T cells, and viral supernatant was harvested to determine the *** treatment with or without doxycycline, HepG2 cells infected with virus were harvested and the expression of iASPP was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot *** effects on tumor growth were characterized using MTS assay, soft agar colony formation, and flow cytometry ***:The lentiviral vector expressing shRNA that targets to the oncogene iASPP was constructed ***2 infected with the lentivirus expressing shRNA against iASPP inhibited the expression of iASPP in the presence of doxycycline, which resulted in the repression of tumor cell proliferation and anchorage-independent growth ***:The lentiviral vector-mediated tet-on system demonstrates efficient and inducible knockdown of iASPP in hepatocellular carcinoma *** gene may be involved in tumorigenesis and progression of human tumors.

关键词：强力霉素载体介导肝癌细胞 RNA干扰基因定位诱导 HepG2细胞逆转录聚合酶链反应

Evolution Mechanism of Calcium Carbonate in Solution

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Chinese Journal of Chemical Physics 2010年第6期23卷 731-737,746页

作者：郭亚平唐海雄周玉贾德昌宁聪琴郭亚军上海师范大学化学系上海200234 哈尔滨工业大学特种陶瓷研究所哈尔滨150001 中国科学院上海硅酸盐研究所高性能陶瓷和超微结构国家重点实验室上海200050

Calcium carbonate was synthesized in a CaCl2/NaCO3 mixed solution by using ethylenediaminetetraacetic acid （EDTA） as an additive. The thermodynamics and kinetics analyses indicate that although the driving force of amorphous calcium carbonate （ACC） precipitation is always less than that of calcite and vaterite precipitation, the nucleation rate of ACC is greater than that of calcite and vaterite at the initial stage of the precipitation reaction. With the increasing incubation time, vaterite and calcite particles nucleate heterogeneously by using the as-formed particles as active sites. Scanning electron microscopy images indicate that the transformation mechanism of ACC and vaterite to calcite is the dissolution-recrystallisation reaction. The presence of EDTA not only improves the stabilities of ACC and vaterite, but also leads to forming enlongated, connected rhombohedral calcite crystals after incubation 7 days in solutions. The ACC and vaterite are stabler in air than in solutions at room temperature, although the dissolution-recrystallisation reaction occurs on the surface.

关键词： Calcium carbonate Dissolution-recrysatllisation reaction Phase transition

星点设计-效应面法优化太白楤木总皂苷磷脂复合物制备工艺

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中药材 2016年第6期39卷 1361-1364页

作者：翟秉涛李佳柳小莉杨伟平郑蓓史亚军郭东艳陕西中医药大学陕西咸阳712046

目的:采用星点设计-效应面法优化太白楤木总皂苷磷脂复合物(TSAT-PC)的制备工艺,并对其理化性质进行研究。方法:采用溶剂挥发法制备TSAT-PC,单因素试验考察反应溶剂、反应时间、反应温度、投料比、药物浓度对复合率的影响,星点设计-效... 详细信息

目的:采用星点设计-效应面法优化太白楤木总皂苷磷脂复合物(TSAT-PC)的制备工艺,并对其理化性质进行研究。方法:采用溶剂挥发法制备TSAT-PC,单因素试验考察反应溶剂、反应时间、反应温度、投料比、药物浓度对复合率的影响,星点设计-效应面法优化制备工艺,并对形成的磷脂复合物进行理化性质研究。结果:最佳制备工艺条件:反应溶剂反应时间1 h、反应温度45℃、大豆磷脂(SL)与TSAT的投料比3∶1、药物浓度16 mg/m L,复合率可达97.23%,与预测值偏差<5%;IR验证了磷脂复合物的形成,其在正辛醇中的溶解度有了较大提高。结论:采用星点设计-效应面法成功制备了TSAT-PC,改善了脂溶性,为中药制剂的进一步开发利用提供了参考。

关键词：太白楤木总皂苷磷脂复合物星点设计-效应面法