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Associations of Sarcopenia, Handgrip Strength and Calf Circumference with Cognitive Impairment among Chinese Older Adults

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Biomedical and Environmental Sciences 2021年第11期34卷 859-870页

作者： WU Bing LYU yue Bin CAO Zhao Jin WEI Yuan SHI Wan Ying GAO Xiang zhou Jin Hui KRAUS Virginia Byers ZHAO Feng CHEN Xin LU Feng zhang ming Yuan LIU Ying Chun TAN Qi yue SONG Shi Xun QU Ying Li ZHENG Xu Lin SHEN Chong MAO Chen SHI Xiao ming Center for Global Health School of Public HealthNanjing Medical UniversityNanjing 211166JiangsuChina china CDC Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China School of Public Health Jilin UniversityChangchun 130012JilinChina department of Nutritional Sciences Pennsylvania State UniversityUniversity ParkPA19019USA Duke Molecular Physiology Institute and department of Medicine Duke University School of MedicineDurhamNorth Carolina27701USA beijing Municipal Health Commission Information Center Beijing 100034China Division of Epidemiology School of Public HealthSouthern Medical UniversityGuangzhou 510515GuangdongChina

Objective To evaluate the associations of sarcopenia,handgrip strength and calf circumference with cognitive impairment among Chinese older *** Totally 2,525 older adults were recruited from the Healthy Aging and Biomarkers Cohort *** impairment was assessed by the Chinese Mini-Mental State *** strength was calculated from the means of the right and left hand *** circumference was measured at the site of maximum circumference of the non-dominant *** formula developed by Ishii was used to define *** logistic regression was performed to evaluate the associations of sarcopenia,handgrip strength,and calf circumference with cognitive *** The prevalence of cognitive impairment was 34.36%.The adjusted odds ratio(OR)for cognitive impairment in individuals with sarcopenia was 2.55[95% confidence interval(95%CI):1.86-3.50].Compared with individuals in the first quartile(Q1)of calf circumference,the adjusted ORs in the second,third,and fourth quartiles(Q_(2),Q_(3),and Q_(4))were 0.75(95% CI:0.58-0.96),0.59(95% CI:0.44-0.79),and 0.62(95% CI:0.45-0.8),*** with individuals in Q1 of handgrip strength,the adjusted ORs for Q_(2),Q_(3),and Q_(4) were 0.49(95%CI:0.38-0.62),0.31(95% CI:0.23-0.41),and 0.30(95%CI:0.21-0.44),*** Sarcopenia,identified by low handgrip strength and low calf circumference,was positively associated with cognitive impairment.

关键词： Sarcopenia Cognitive impairment Handgrip strength Calf circumference Older adults

A Dandy-Walker malformation associated with ganglioglioma

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Chinese Medical Journal 2019年第20期132卷 2495-2497页

作者： Lei-ming Wang Meng zhang Pei-Pei Wang Xin-Gang zhou yue-Shan Piao De-Hong Lu department of Pathology Xuanwu HospitalCapital Medical UniversityBeijing 100053China department of Radiology Xuanwu HospitalCapital Medical UniversityBeijing 100053China department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China

To the Editor: The Dandy-Walker malformation (DWM) is a rare congenital malformation involving the posterior fossa. Diagnosis of the DWM is based on a series of characteristic neuroimaging findings, which include complete or partial agenesis of the cerebellar vermis, cystic dilatation of the fourth ventricle, and enlarged posterior fossa.[1] Several malformation syndromes and cytogenetic abnormalities have been associated with the DWM. However, the co-existence of the DWM and neoplasms is rare.[2] There are rare reports of the co-existence of the DWM and ganglioglioma.

关键词： malformation neoplasms glioma

天语计划——寻找第二个太阳系及探索动态宇宙

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天文学报 2024年第4期65卷 1-43页

作者：冯发波芮易成杜芝茂林清张聪聪周丹崔开明 OGIHARA Masahiro 杨明林杰蔡永志杨涛只庞晓莹简明杰李文雄郭恒潇史弦史建春李荐扬郭康柔姚嵩陈啊明贾鹏谭先瑜 JENKINS S.James 蒋宏轩张铭缘李可欣肖光耀郑书玥宣一帆郑捷何敏 JONES R.A.Hugh 宋翠英上海交通大学李政道研究所上海201210 上海交通大学物理与天文学院上海200240 上海天文馆(上海科技馆分馆) 上海200127 中国科学院上海天文台上海200030 同济大学测绘与地理信息学院上海200092 中国科学院星系宇宙学重点实验室(中国科学技术大学) 合肥230026 中国科学技术大学天文与空间科学学院合肥230026 中国科学院云南天文台昆明650216 中国科学院天体结构与演化重点实验室昆明650216 国际超新星中心-云南省国际超新星研究重点实验室昆明650216 西安交通大学物理学院西安710049 西交利物浦大学物理系苏州215123 上海师范大学上海市星系与宇宙学半解析研究重点实验室上海200234 department of Astronomy Stockholm University Stockholm11421 中国科学院国家天文台北京100101 中山大学大气科学学院珠海519082 太原理工大学电子信息与光学工程学院太原030024 Instituto de Estudios Astrofısicos Facultad de Ingenierıa y CienciasUniversidad Diego Portales Santiago8170641 Centro de Astrof´ısica y Tecnologıas Afines(CATA)Santiago 8480000 山东大学空间与物理学院山东省光学天文与日地空间环境重点实验室威海264209 中国科学院国家天文台中国科学院天文光学技术重点实验室北京100101 Centre for Astrophysics Research University of Hertfordshire HertfordshireAL109AB 清华大学天体物理研究中心北京100084

像木星和土星这样的冷巨行星对类地行星的形成和宜居性起到了关键作用,因此,对像太阳系这样具有多个冷巨行星的系统的搜寻具有重要意义.Kepler以及TESS (Transiting Exoplanet Survey Satellite)太空望远镜成功地通过凌星的方法发现了... 详细信息

像木星和土星这样的冷巨行星对类地行星的形成和宜居性起到了关键作用,因此,对像太阳系这样具有多个冷巨行星的系统的搜寻具有重要意义.Kepler以及TESS (Transiting Exoplanet Survey Satellite)太空望远镜成功地通过凌星的方法发现了大量短周期行星,然而这些巡天项目的观测基线不足以发现更长周期的行星.为此,天语计划将部署两台1m望远镜(天语一号和天语二号),用于结合天语和其他计划的数据通过凌星法来发现冷巨行星及其他行星,并最终发现类太阳系.天语一号具有约10平方度的视场以及高速CMOS (complementary metal-oxide-semiconductor)科学相机,将对超过1000万颗恒星每小时进行一次高精度测光采样,并通过天语二号的多波段测光、光谱仪以及高分辨率成像对天语一号发现的候选行星进行确认.天语望远镜将放置于海拔约4000m的中国青海冷湖,是目前中国境内最好观测台址之一.天语对亮于14等星的测光精度为0.1%,对亮于18等星的测光精度优于1%,天语预计在5yr内将在类太阳周期发现超过300颗凌星行星,其中包括约17颗冷巨行星.基于系统轨道共面以及孪生地球发生率为10%的假设,天语预计将发现1-2个类太阳系,并可被未来的地球2.0计划所证实.此外,天语还将通过多种巡天观测模式测量从亚秒到周时标的光变,探测不同时标的时域现象,包括超新星早期光变、稀有变星和双星、潮汐瓦解事件、Be星、彗星活动以及系外小行星等.这些发现不仅将深化我们对宇宙的理解,还将为公众科学和科普提供重要平台.

关键词：技术:测光行星与卫星:探测行星与卫星:形成恒星:超新星:普通恒星:变星:普通小行星:普通恒星:伽马射线暴:普通

Impact factors of Blastocystis hominis infection in persons living with human immunodeficiency virus:a large-scale,multi-center observational study from china

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Infectious Diseases of Poverty 2023年第5期12卷 95-96页

作者： Shun-Xian zhang Ji-Chun Wang Zhong-Wei Li Jin-Xin Zheng Wen-Ting zhou Guo-Bing Yang Ying-Fang Yu Xiu-Ping Wu Shan Lv Qin Liu Mu-Xin Chen Yan Lu Zhi-Hui Dou Da-Wei zhang Wen-Wen Lv Lei Wang Zhen-Hui Lu ming Yang Pei-Yong Zheng yue-Lai Chen Li-Guang Tian Xiao-Nong zhou Longhua Hospital Shanghai University of Traditional Chinese Medicine Shanghai China Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research) NHC Key Laboratory of Parasite and Vector Biology WHO Collaborating Centre for Tropical Diseases National Center for International Research On Tropical Diseases National Institute of Parasitic Diseases Shanghai China School of Global Health Chinese Center for Tropical Diseases Research-Shanghai Jiao Tong University School of Medicine Shanghai China department of Science and Technology Chinese Center for Disease Control and Prevention Beijing China Gansu Province People’s Hospital Gansu Provincial Hospital Lanzhou China National Health Commission (NHC) Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China Gansu Provincial Center for Disease Control and Prevention Lanzhou China National Center for AIDS/STD Control and Prevention China Center for Disease Control and Prevention Beijing China The People’s Liberation Army 302 Hospital Beijing China Clinical Research Institute Shanghai Jiao Tong University School of Medicine Shanghai China

Background Blastocystis hominis(Bh)is zoonotic parasitic pathogen with a high prevalent globally,causing opportunistic infections and diarrhea *** immunodeficiency virus(HIV)infection disrupts the immune system by depleting CD4^(+)T lymphocyte(CD4^(+)T)cell counts,thereby increasing Bh infection risk among persons living with HIV(PLWH).However,the precise association between Bh infection risk and HIV-related biological markers and treatment processes remains poorly ***,the purpose of the study was to explore the association between Bh infection risk and CD4^(+)T cell counts,HIV viral load(VL),and duration of interruption in antiviral therapy among *** A large-scale multi-center cross-sectional study was conducted in china from June 2020 to December *** genetic presence of Bh in fecal samples was detected by real-time fluorescence quantitative polymerase chain reaction,the CD4^(+)T cell counts in venous blood was measured using flowcytometry,and the HIV VL in serum was quantified using fluorescence-based *** cubic spline(RCS)was applied to assess the non-linear association between Bh infection risk and CD4^(+)T cell counts,HIV VL,and duration of interruption in highly active antiretroviral therapy(HARRT).Results A total of 1245 PLWH were enrolled in the study,the average age of PLWH was 43 years[interquartile range(IQR):33,52],with 452(36.3%)being female,50.4%(n=628)had no immunosuppression(CD4^(+)T cell counts>500 cells/μl),and 78.1%(n=972)achieved full virological suppression(HIV VL31)of PLWH had *** prevalence of Bh was found to be 4.9%[95%confidence interval(CI):3.8-6.4%]among *** nonlinear associations were observed between the Bh infection risk and CD4^(+)T cell counts(Pfor nonlinearity<0.001,L-shaped),HIV VL(Pfor nonlinearity<0.001,inverted U-shaped),and duration of interruption in HARRT(Pfor nonlinearity<0.001,inverted U-shaped).Conclusions

关键词： Blastocystis hominis Human immunodeficiency virus Viral load Non-linear association Restricted cubic spline Propensity score china

A flexible adhesive with a conductivity of 5240 S/cm

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Science Bulletin 2021年第7期66卷 657-660,M0003页

作者： Dongwei zhang Sai Liu yue Jiang Yuxin Yin Zhengjie Xu Xiangyu Kong Guofu zhou Jun-ming Liu Krzysztof Kempa Liming Ding Jinwei Gao Guangdong Provincial Key Laboratory of Optical Information Materials and Technology Academy of Advanced OptoelectronicsSouth China Normal UniversityGuangzhou 510006China Laboratory of Solid State Microstructures Nanjing UniversityNanjing 210093China department of Physics Boston CollegeChestnut Hill MA 02467USA Center for Excellence in Nanoscience(CAS) Key Laboratory of Nanosystem and Hierarchical Fabrication(CAS)National Center for Nanoscience and TechnologyBeijing WO 190China Guangdong Provincial Engineering Technology Research Center for Transparent Conductive Materials Guangzhou 510006China

Conductive adhesives(CAs)providing reliable connectionsbetween electronic *** been widely used in solarcells[1],light emitting diodes(LED5)[2],radio frequency compo-nents[3],and printed&wearable ***.[4-6].Basically,CAs are mainly composed of conductive fillers and resin,whichgive the *** *** the conductive path,respectively[7].Among conductive fillers with high conductivity,such as carbon materials[7,8].and metals[4,5.9.10],are the keycomponent[9,10],determining the feature and cost of the CAs.

关键词：导电银胶柔性薄膜光滑表面渗流阈值导电介质电导率导电网络高电导

基于金属-半导体-金属结构的Bi2Te3室温高响应率太赫兹探测器

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红外与毫米波学报 2019年第4期38卷 459-463,472页

作者：徐新月张晓东吴敬江林吴彩阳姚娘娟曲越周炜尹一鸣黄志明东华大学理学院应用物理系上海201620 中国科学院上海技术物理研究所红外物理国家重点实验室上海200083 中国科学院大学北京100049 上海智能电子与系统研究所上海201620

基于二维拓扑绝缘体Bi 2Te 3材料利用微纳工艺制备了金属-拓扑绝缘体-金属(MTM)结构的太赫兹光电探测器.器件在0.022 THz的响应率可达2×10 3 A/W,噪声等效功率(NEP)低于7.5×10 -15 W/Hz 1/2 ,探测率D*高于1.62 ×10 11 cm·Hz 1/2 /W;... 详细信息

基于二维拓扑绝缘体Bi 2Te 3材料利用微纳工艺制备了金属-拓扑绝缘体-金属(MTM)结构的太赫兹光电探测器.器件在0.022 THz的响应率可达2×10 3 A/W,噪声等效功率(NEP)低于7.5×10 -15 W/Hz 1/2 ,探测率D*高于1.62 ×10 11 cm·Hz 1/2 /W;在0.166 THz的响应率可达281.6 A/W,NEP低于5.18×10 -14 W/Hz 1/2 ,D*高于2.2×10 10 cm·Hz 1/2 /W;在0.332 THz的响应率可达7.74 A/W,NEP低于1.75×10 -12 W/Hz 1/2 ,D*高于6.7 ×10 8 cm ·Hz 1/2 /W;同时器件在太赫兹波段具有小的时间常数(7~8 μs).该项工作突破了传统光子探测的带间跃迁,实现了可室温工作、高响应率、高速响应以及高灵敏度的太赫兹探测器件.

关键词：太赫兹探测器二维材料 Bi2Te3

Timing analysis of the black hole candidate EXO 1846–031 with Insight-HXMT monitoring

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Research in Astronomy and Astrophysics 2021年第3期21卷 193-204页

作者： He-Xin Liu yue Huang Guang-Cheng Xiao Qing-Cui Bu Jin-Lu Qu Shu zhang Shuang-Nan zhang Shu-Mei Jia Fang-Jun Lu Xiang Ma Lian Tao Wei zhang Li Chen Li-ming Song Ti-Pei Li Yu-Peng Xu Xue-Lei Cao Yong Chen Cong-Zhan Liu Ce Cai Zhi Chang Gang Chen Tian-Xiang Chen Yi-Bao Chen Yu-Peng Chen Wei Cui Wei-Wei Cui Jing-Kang Deng Yong-Wei Dong Yuan-Yuan Du Min-Xue Fu Guan-Hua Gao He Gao Min Gao ming-Yu Ge Yu-Dong Gu Ju Guan Cheng-Cheng Guo Da-Wei Han Jia Huo Lu-Hua Jiang Wei-Chun Jiang Jing Jin Yong-Jie Jin Ling-Da Kong Bing Li Cheng-Kui Li Gang Li Mao-Shun Li Wei Li Xian Li Xiao-Bo Li Xu-Fang Li Yang-Guo Li Zheng-Wei Li Xiao-Hua Liang Jing-Yuan Liao Bai-Sheng Liu Guo-Qing Liu Hong-Wei Liu Xiao-Jing Liu Yi-Nong Liu Bo Lu Xue-Feng Lu Qi Luo Tao Luo Bin Meng Yi Nang Jian-Yin Nie Ge Ou Na Sai Ren-Cheng Shang Xin-Ying Song Liang Sun Ying Tan You-Li Tuo Chen Wang Guo-Feng Wang Juan Wang Ling-Jun Wang Weng-Shuai Wang Yu-Sa Wang Xiang-Yang Wen Bai-Yang Wu Bo-Bing Wu Mei Wu Shuo Xiao Shao-Lin Xiong He Xu Jia-Wei Yang Sheng Yang Yan-Ji Yang Yi-Jung Yang Qi-Bin Yi Qian-Qing Yin Yuan You Ai-Mei zhang Cheng-Mo zhang Fan zhang Hong-Mei zhang Juan zhang Tong zhang Wan-Chang zhang Wen-Zhao zhang Yi zhang Yi-Fei zhang Yong-Jie zhang Yuan-Hang zhang yue zhang Zhao zhang Zhi zhang Zi-Liang zhang Hai-Sheng Zhao Xiao-Fan Zhao Shi-Jie Zheng Yao-Guang Zheng Deng-Ke zhou Jian-Feng zhou Yu-Xuan Zhu Ren-Lin Zhuang yue Zhu Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China university of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100049China Institut fur Astronomie und Astrophysik Kepler Center for Astro and Particle PhysicsEberhard Karls Universität72076 TubingenGermany department of Astronomy Beijing Normal UniversityBeijing 100088China department of Astronomy Tsinghua UniversityBeijing 100084China department of Physics Tsinghua UniversityBeijing 100084China department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Space Astronomy and Technology National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China College of Physics Sciences&Technology Hebei UniversityBaoding 071002Hebei ProvinceChina

We present the observational results from a detailed timing analysis of the black hole candidate EXO 1846-031 during its outburst in 2019 with the observations of Insight-HXMT,NICER and *** outburst can be classified roughly into four different ***-C quasi-periodic oscillations(QPOs)observed by NICER(about 0.1-6 Hz)and Insight-HXMT(about 0.7-8 Hz)are also reported in this ***,we study various physical quantities related to QPO *** QPO rms-frequency relationship in the energy band 1-10 keV indicates that there is a turning pointing in frequency around2 Hz,which is similar to that of GRS 1915+105.A possible hypothesis for the relationship above may be related to the inclination of the source,which may require a high inclination to explain *** relationships between QPO frequency and QPO rms,hardness,total fractional rms and count rate have also been found in other transient sources,which can indicate that the origin of type-C QPOs is non-thermal.

关键词： X-ray binaries black hole candidate QPO EXO 1846–031

Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An evidence-based clinical practice guideline(updated version)

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Military Medical Research 2020年第3期7卷 249-282页

作者： Ying-Hui Jin Qing-Yuan Zhan Zhi-Yong Peng Xue-Qun Ren Xun-Tao Yin Lin Cai Yu-Feng Yuan Ji-Rong yue Xiao-Chun zhang Qi-Wen Yang Jianguang Ji Jian Xia Yi-Rong Li Fu-Xiang zhou Ya-Dong Gao Zhui Yu Feng Xu ming-Li Tu Li-ming Tan Min Yang Fang Chen Xiao-Ju zhang Mei Zeng Yu Zhu Xin-Can Liu Jian Yang Dong-Chi Zhao Yu-Feng Ding Ning Hou Fu-Bing Wang Hao Chen Yong-Gang zhang Wei Li Wen Chen yue-Xian Shi Xiu-Zhi Yang Xue-Jun Wang Yan-Jun Zhong ming-Juan Zhao Bing-Hui Li Lin-Lu Ma Hao Zi Na Wang Yun-Yun Wang Shao-Fu Yu Lu-Yao Li Qiao Huang Hong Weng Xiang-Ying Ren Li-Sha Luo Man-Ru Fan Di Huang Hong-Yang Xue Lin-Xin Yu Jin-Ping Gao Tong Deng Xian-Tao Zeng Hong-Jun Li Zhen-Shun Cheng Xiao-Mei Yao Xing-Huan Wang Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China National Clinical Research Center for Respiratory Diseases China-Japan Friendship HospitalBeijing 100029China department of Pulmonary and Critical Care Medicine China-Japan Friendship HospitalBeijing 10029China Leishenshan Hospital in Wuhan Wuhan 430200China department of Critical Care Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China Institutes of Evidence-based Medicine and Knowledge Translation Henan UniversityKaifeng 475000HenanChina department of Medical Imaging Guizhou Provincial People’s HospitalGuiyang 550002China departments of Orthopedics Zhongnan Hospital of Wuhan UniversityWuhan 430071China department of Hepatobiliary Surgery Zhongnan Hospital of Wuhan UniversityWuhan 430071China National Clinical Research Center for Geriatrics West China Hospital of Sichuan UniversityChengdu 610041China department of Geriatrics West China Hospital of Sichuan UniversityChengdu 610041China department of Radiology Zhongnan Hospital of Wuhan UniversityWuhan 430071China department of Clinical Laboratory Peking Union Medical College Hospital(PUMCH)Peking Union Medical CollegeChinese Academy of Medical Sciences(CAMS)Beijing 100730China Center for Primary Health Care Research Lund University and Region Skåne25002 MalmöSweden Emergency Center Zhongnan Hospital of Wuhan UniversityWuhan 403371China department of Allergology Zhongnan Hospital of Wuhan UniversityWuhan 430071China department of Radiation and Medical Oncology Zhongnan Hospital of Wuhan UniversityWuhan 43071China department of Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhan 430060China department of Emergency Medicine and Chest Pain Center Qilu Hospital of Shandong UniversityJinan 250002China department of Respiratory and Critical Care Medicine Suizhou Central HospitalHubei University of MedicineSuizhou 441300HubeiChina department of Clinic Pharmacy Second People’s Hospital of Huaihua Ci

The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the cause of a rapidly spreading illness,coronavirus disease 2019(COVID-19),affecting more than seventeen million people around the *** and treatment guidelines for clinicians caring for patients are *** the early stage,we have issued"A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV)infected pneumonia(standard version)";now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based *** formed a working group of clinical experts and *** steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas:chemoprophylaxis,diagnosis,treatments,and discharge *** searched the literature for direct evidence on the management of COVID-19,and assessed its certainty generated recommendations using the Grading of Recommendations,Assessment,Development and Evaluation(GRADE)*** were either strong or weak,or in the form of ungraded consensus-based ***,we issued 34 *** them,6 were strong recommendations for,14 were weak recommendations for,3 were weak recommendations against and 11 were ungraded consensus-based *** covered topics of chemoprophylaxis(including agents and Traditional Chinese Medicine(TCM)agents),diagnosis(including clinical manifestations,reverse transcription-polymerase chain reaction(RT-PCR),respiratory tract specimens,IgM and IgG antibody tests,chest computed tomography,chest X-ray,and CT features of asymptomatic infections),treatments(including lopinavirritonavir,umifenovir,favipiravir,interferon,remdesivir,combination of antiviral drugs,hydroxychloroquine/chloroquine,interleukin-6 inhibitors,interleukin-1 inhibitors,glucocorticoid,qingfei paidu decoction,lianhua qingwen granules/capsules,convalescent plasma,lung

关键词： COVID-19 SARS-CoV-2 Recommendation Chemoprophylaxis Diagnosis Treatment Discharge management Traditional Chinese medicine guideline

miR-203a-5p靶向调控JAG1对多发性骨髓瘤细胞增殖、周期调控的作用及机制研究

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中国实验血液学杂志 2023年第3期31卷 801-809页

作者：张越陈婷婷周合冰陈文明首都医科大学附属北京潞河医院血液科北京101149 首都医科大学附属北京朝阳医院血液科北京市多发性骨髓瘤医疗研究中心北京100020

目的:探讨miR-203a-5p在多发性骨髓瘤(MM)中的生物学功能及其调控机制。方法:采用稳健排序整合方法对GEO数据库中的3个miRNA表达谱(GSE16558、GSE24371和GSE17498,包含131个MM样本和17个正常浆细胞样本)进行差异性表达整合分析。用慢病... 详细信息

目的:探讨miR-203a-5p在多发性骨髓瘤(MM)中的生物学功能及其调控机制。方法:采用稳健排序整合方法对GEO数据库中的3个miRNA表达谱(GSE16558、GSE24371和GSE17498,包含131个MM样本和17个正常浆细胞样本)进行差异性表达整合分析。用慢病毒构建了能稳定过表达miR-203a-5p的MM细胞株。采用qRT-PCR方法检测MM细胞株RPMI8226及U266中miR-203a-5p的表达情况,并检测miR-203a-5p表达上调后对MM细胞增殖及细胞周期表型的影响。应用稳定表达miR-203a-5p的U226细胞进行裸鼠成瘤实验,评价miR-203a-5p在体内肿瘤发生中的作用。利用Target Scan和miRanda等生物预测软件预测miR-203a-5p的候选靶基因,利用荧光素酶报告基因实验、qRT-PCR和Western blot研究MM细胞中miR-203a-5p的潜在靶点。结果:miR-203a-5p在MM细胞系中的表达显著低于正常浆细胞。miR-203a-5p过表达可以抑制RPMI8226和U266细胞的增殖和细胞周期进展。动物体内实验表明,上调miR-203a-5p可以明显抑制体内肿瘤的生长。通过双荧光素酶报告基因实验证实了JAG1的3’UTR区域可以与miR-203a-5p结合,JAG1是miR-203a-5p的作用靶基因。此外,回复实验结果显示,过表达JAG1可以逆转由miR-203a-5p引起的细胞增殖抑制及周期阻滞。结论:miR-203a-5p通过靶向调控JAG1抑制多发性骨髓瘤细胞的增殖和细胞周期进展,从而发挥抑癌作用,该分子有望作为一种基于miRNA的MM治疗靶点。

关键词：多发性骨髓瘤 miR-203a-5p JAG1 增殖细胞周期