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Axon guidance and neuronal migration research in china

Science china(Life sciences) 2010年第3期53卷 304-314页

作者： yuan xiaobing institute of neuroscience,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 1. institute of neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Proper migration of neuronal somas and axonal growth cones to designated locations in the developing brain is essential for the assembly of functional neuronal *** progress in research of axon guidance and neuronal migration has been made in the last twenty *** researchers began their exploration in this field ten years ago and have made significant contributions in clarifying the signal transduction of axon guidance and neuronal *** unique experimental approaches,including the migration assay of single isolated neurons in response to locally delivered guidance cues,have been developed by chinese neuroscientists to investigate the molecular machinery underlying these guidance events.

关键词： axon guidance neuronal migration development neuronal circuits

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Essential role of PKC-delta in radial migration of cortical neurons

Essential role of PKC-delta in radial migration of cortical ...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Chun-tao ZHAO,Xiao-bing yuan Laboratory of Neural Circuit Development,institute of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai,200031

＜正＞The layered structure of the mammalian neocortex is formed through a well-coordinated radial migration of cohorts of post-mitotic neurons during early *** proteins have been identified as essential for the migration of neurons and the formation of stereotyped cortical ***,how the newborn neurons are regulated to migrate or to terminate their migration at their destined layers remains *** we examined the contribution of a member of novel PKC in the migration of newborn neurons to their final layer in developing rat cortex. Using in utero electroporation of small interference RNAs（siRNA）,we found that specific down-regulation of PKC-delta severely impaired the targeting of the newborn neurons to their destined layers,without affecting proliferation of neural progenitor cells or the differentiation of newborn *** also found that down-regulation of cdk5 lead to similar migration defect to that of *** antibody against p35 immunoprecipitated ***,brain- neurotrophic factor（BDNF） strongly increased the phosphorylation level of both PKC-delta and *** of TrkB receptor also lead to migration defects of cortical ***,the PKC-delta signaling may interact with p35-cdk5 complex and regulate the proper radial migration of newborn cortical neurons.

关键词： radial migration cortex PKC-delta P35 TrkB

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PlexinA2 is essential for migration of cerebellar granule cells from EGL toIGL

PlexinA2 is essential for migration of cerebellar granule ce...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Jian SIMA,Kai CUI,Xiao-Bing yuan Laboratory of Neural Circuit Development,institute of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031

＜正＞In the early postnatal cerebellum,postmitotic granule cells migrate from the external granular layer（EGL） to the internal granular layer（IGL） along radial glial *** this migration event has been well described,the underlying cellular and molecular mechanisms remain largely *** have developed a method of electroporation to reliably introduce DNA constructs into granule cell precursors in the superficial part of EGL in vivo and trace the migration and positioning of granule cells after interference with the expression of specific *** results showed that downregulation of plexinA2 in cerebellar granule cells disrupted their positioning during development with defects in the radial migration and the neurite extension.

关键词： cerebellum granule cell migration plexin A2

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Nestin promotes the proliferation of neural progenitor cells by negatively regulating tumor suppressor PTEN

Nestin promotes the proliferation of neural progenitor cells...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xiao-Jing XUE~(1,2),Xiao-Bing yuan~(1,*) 1 institute of neuroscience and State Key Laboratory of Neurobiology,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china 2 Graduate School of the chinese academy of sciences,shanghai 200031,china

＜正＞The intermediate filament protein Nestin is a widely-accepted molecular marker for neural progenitor cells （NPCs）,but its function during brain development remains an *** explore the potential function of Nestin in neurogenesis,we down-regulated its expression in NPCs in developing cerebral cortex by using the in utero electroporation of specific small interference RNAs（siRNA）.We found that the down-regulation of the expression of Nestin,but not its co-ploymer protein Vimentin,in cortical NPCs resulted in a severe reduction in the genesis of neurons,accompanied with a cell-cycle arrest of affected *** the primary culture of cortical NPCs,down-regulation of Nestin markedly suppressed the colony-formation ability of NPCs and blocked the elevation of cylcinD1/E in response to the mitogen bFGF. Interestingly,the down-regulation of Nestin casued a marked increase in the protein level of the phosphatase and tensin homolog PTEN,and correspondingly suppressed the activation of the phosphoinositide 3-kinase signaling pathway but not the mitogen-activated protein kinase ***,defects in the proliferation of cortical NPCs caused by the down-regulation of Nestin could be mimicked by the overexpression of PTEN and compensated by simultaneous down- regulation of PTEN both in vivo and in ***,Nestin may promote the proliferation of NPCs by negatively regulating the tumor suppressor PTEN.

关键词： Nestin PTEN neural progenitor cells proliferation siRNA

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The role of slit-robo signaling in cortical neuronal migration

The role of slit-robo signaling in cortical neuronal migrati...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Jian SIMA,Kai CUI,Zhi-Hui HUANG,Xiao-Bing yuan Laboratory of Neural Circuit Development,institute of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031

＜正＞During early development of the vertebrate cerebral cortex,newborn neurons migrate along radial glia filaments from the ventricular zone into the cortical plate before arriving at the proper layer and forming appropriate synaptic *** molecular mechanisms underlying the guidance of radial migration remain largely *** in utero electroporation of small interference RNAs（siRNA）,we found that knock down the expression of robo3,one of the receptors of the guidance factor Slit,lead to the failure of newborn neurons to enter the cortical plate and the failure of the midline crossing of axons of transfected ***3 is unlikely to mediate an attractive signal from slit since ectopic neurons in deep cortical layers after silencing robo3 expression displayed normal radial ***-down of robo2 had no effect on radial migration,but remarkably simplified dendrites of cortical neurons after they have arrived at the final ***,co-transfection of the siRNA against robo2 with that of robo3 partially rescued the migration defect caused by transfection of robo3 siRNA *** in-situ hybridization,we found that Slitl is expressed in laminar patter in cortical plate,consistent with the previous *** Slit receptor robo2 is expressed in cortical neurons at both embryonic and postnatal cortical neurons,wheares robo3 is only transiently expressed in newborn *** on these findings and the known effect of robo3 to silence the repulsive action of Slit mediated by robo1/2,we hypothesize that robo3 plays essential roles in the radial migration by silencing the repulsive effect of slitl expressing in cortical plate and allows newborn neurons to migrate from the intermediate zone into the cortical *** studies using in vitro migration assays will be required to further test this hypothesis.

关键词： neuronal migration slit robo2 robo3

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Non-apoptotic role of caspase-3 in synapse refinement

Non-apoptotic role of caspase-3 in synapse refinement

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Fei CHEN,Jin-yuan WANG,Nan-Jie RUAN,Lei QIAN,Bao-Yu DUAN,Zhen-Ge LUO~* institute of neuroscience and Key Laboratory of Neurobiology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china

＜正＞During the development of vertebrate neuromuscular junction,the formation and maintenance of acetylcholine receptor（AChR） clusters are regulated by both positive and negative *** stabilizes,whereas acetylcholine（ACh） destabilizes AChR *** mechanisms underlying this counteractive interaction are not completely *** we show that caspase-3,an effector caspase involved in apoptosis,negatively regulates AChR *** found that caspase-3 was activated by cholinergic stimulation of cultured muscle *** activation did not depend on calpain,a calcium-dependent protease which is involved in the dispersion of AChR *** treatment prevented the activation of ***,inhibition or down-regulation of caspase-3 attenuated ACh agonist-induced dispersion of AChR ***,the loss of AChR clusters in agrin mutant mice was partially rescued by the inhibition of caspase-3 or genetic ablation of *** together, these results suggest a novel role of caspase-3 in the refinement of synapse.

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Lipid rafts mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X3 receptor

Lipid rafts mediates ATP-induced endocytosis,signal transduc...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xu-Qiao CHEN~(1,#),Bin WANG~(1,#),Chengbiao WU~2,Jin PAN~1,Bo yuan~3,yuan-yuan SU~1,Xing-Yu JIANG~3,Xu ZHANG~4 Lan BAO~(1,*) 1 Laboratory of Molecular Cell Biology,institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 2 Department of neurosciences,School of Medicine,University of California,San Diego,La Jolla,California,USA 3National Center of Nanoscience and Technology,Beijing 100190,china 4 institute of neuroscience and State Key Laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china

P2X receptors are a family of cation channels expressed in neurons and nonneuronal *** play a variety of roles including synaptic transmission,muscle contraction,neuronal secretion and inflammation response. Among P2X receptors,the P2X subtype is selectively expressed in dorsal root ganglion *** raft is a specialized membrane microdomain and participate many signal transduction *** report showed that P2X receptor is localized in the lipid rafts of DRG *** have found P2X receptor engaged in retrograde transport in the form of signaling *** we investigated the role of lipid rafts in a,B-MeATP-dependent endocytosis and signal transduction,and retrograde transport of P2X *** of lipid raft with MBCD or nystatin abolished ATP-dependent endocytosis of P2X receptor and ATP-induced ERK activation in P2X receptor-expressing HEK293 *** vivo sucrose gradient fractionation with vesicular pellets prepared from sciatic nerve axoplasm showed that abundant P2X receptor was localized in lipid raft *** transported P2X receptors were colocalized with the lipid raft marker caveolinla in sciatic *** terminal treatment with MPCD attenuated retrograde transport of P2X receptor in axon of dorsal root ganglion ***,lipid raft mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X receptor.

关键词： P2X3 receptor lipid raft endocytosis ERK retrograde transport

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ATP-dependent retrograde axonal transport of P2X3 receptor in primary sensory neurons

ATP-dependent retrograde axonal transport of P2X3 receptor i...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Bin WANG~1,Xu-Qiao CHEN~1,Cheng-Biao WU~2,Jin PAN~1,Bo yuan~3,yuan-yuan SU~1,Xing-Yu JIANG~3, Xu ZHANG~4,Lan BAO~(1,*) 1 Laboratory of Molecular Cell Biology,institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 2 Department of neurosciences,School of Medicine,University of California,San Diego,La Jolla,California 92093- 0662,USA 3 National Center for Nanoscience and Technology,Beijing 100080,china 4 institute of neuroscience and State Key Laboratory of neuroscience,institutes for biological sciences,chinese academy of sciences,shanghai 200031,china

Objective P2X receptors are ATP-gated cation channels and play variable roles in pathophysiological *** P2X receptors,P2X

关键词： primary sensory neuron P2X3 receptor axonal retrograde transport

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Deficiency of miR-146 Contributes to Abnormal Activation of the TypeI Interferon Pathway in Systemic Lupus Erythematosus

Deficiency of miR-146 Contributes to Abnormal Activation of ...

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第六届全国免疫学学术大会

作者： yuanjia Tang xiaobing Luo Huijuan Cui Xuming Ni Min yuan Yanzhi Guo Xinfang Huang Haibo Zhou Shunle Chen Nan Shen Joint Molecular Rheumatology Laboratory of shanghai Ren Ji Hc,spital and institute of Health sciences,shanghai institutes for biological sciences,chinese academy of sciences and shanghai JiaoTong University School of Medicine,shanghai,china

MicroRNAs have been recently identified as regulators that modulate target gene expression and are involved in shaping immune *** study was undertaken to investigate the contribution of miR-146a,which was identified i...

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Neuregulinl represses limbic epileptogenesis via activation of ErbB4 signaling in parvalbumin-positive interneurons

Neuregulinl represses limbic epileptogenesis via activation ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Guo-He TAN~(1,★),yuan-yuan LIU~(1,★),Xiao-Ling HU~1,Dong-Min YIN~2,Lin MEI~(2,*),Zhi-Qi XIONG~(1,*) 1 institute of neuroscience and State Key Laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china 2 Program of Developmental Neurobiology and Department of Neurology,institute of Molecular Medicine and Genetics,Georgia’s Health sciences University,Augusta,GA 30912,USA

Objective Epilepsy is a common and refractory neurological disorder that affects l%-2%worldwide people,but the neuronal regulatory mechanisms of epileptogenesis remain largely ***-dependent expression of polypeptide growth factors has been implicated in epileptogenesis;however,little is known about factors that may act as intrinsic,homeostatic or counter-balancing *** this study,we investigated the role of a growth factor neuregulin 1(NRG1) and its ErbB4 receptor in limbic *** Two classical models of limbic epilepsy,kindling and pilocarpine models,were used to investigate the expression pattern of NRG1 and ErbB4. Using lose- and gain- of- function strategies,we determined the role of NRG1/ErbB4 signaling in epileptogenesis pharmacologically and ***/loxP-mediated cell-specific mutation was employed to identify the specific cellular mechanism underlying the effect of this signaling in limbic ***(1) Limbic seizure activity increased the expression of Nrg1 and phosphorylation of ErbB4 receptor in the brain.(2) In kindling model, epileptogenesis was inhibited by intracerebral infusion of recombinant NRG1,but exacerbated by neutralizing endogenous NRG1 with soluble ecto-ErbB4.(3) Pharmacologically,inhibition of ErbB4 activity with PD158780 greatly promoted kindling-induced epileptogenesis.(4) Genetically,heart-rescued ErbB4 knockout mice exhibited accelerated progression of the kindling effect.(5) Specific depletion of ErbB4 in parvalbumin(PV)-positive GABAergic interneurons resulted in accelerated kindling ***,NRG1 infusion failed to suppress kindling development in PV-Cre;ErbB4 mice.(6) Deletion of ErbB4 in CaMKIIα-positive pyramidal neurons was ineffective for epileptogenesis,as evidenced by normal kindling progression of both CaMKIIα-iCre;ErbB4 and CaMKIIα-CreER;ErbB4 mice.(7) Ablation of ErbB4 in PV-positive neurons increased kindling-induced brain hyperexcitability and spo

关键词： limbic epilepsy epileptogenesis neuregulin 1 ErbB4 parvalbumin-positive interneurons

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