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贝氏柯克斯体耐受渗透脱水研究模型的建立

中国人兽共患病学报 2024年第5期40卷 415-420页

作者：王涛孙静尹白露于永慧何卫平段学章解放军总医院第五医学中心肿瘤医学部放射治疗科北京100039 解放军总医院第五医学中心感染病医学部国家感染临床医学研究中心北京100039 军事医学研究院微生物流行病研究所病原微生物生物安全国家重点实验室北京100071

目的基于无生命培养体系,探索建立贝氏柯克斯体耐受脱水的实验室研究模型。方法梯度调整贝氏柯克斯体无生命培养体系中的渗透压条件,利用qPCR定量测定不同渗透压下贝氏柯克斯体在培养周期内基因组拷贝数变化并描记生长曲线,进一步利用... 详细信息

目的基于无生命培养体系,探索建立贝氏柯克斯体耐受脱水的实验室研究模型。方法梯度调整贝氏柯克斯体无生命培养体系中的渗透压条件,利用qPCR定量测定不同渗透压下贝氏柯克斯体在培养周期内基因组拷贝数变化并描记生长曲线,进一步利用相差显微镜、透射电子显微镜对真核细胞以及无细胞培养方法所得贝氏柯克斯体进行形态学分析,并感染BGMK细胞测定不同渗透压培养所得贝氏柯克斯体的感染性VFU。结果贝氏柯克斯体可在高渗透压条件的无生命培养基中适应性生存7 d以上,经高渗透压培养后的菌体脱水收缩且大小类似于小贝氏体,同时感染后24 h的VFU较对照组降低。结论成功建立了贝氏柯克斯体耐受高渗压脱水的研究模型,为下一步用蛋白质组学等方法研究贝氏柯克斯体在自然环境中耐受干燥脱水的遗传学机制奠定了基础。

关键词：贝氏柯克斯体 Q热渗透压脱水无生命培养

政策工具视角下我国2003-2021年人兽共患病防控政策文本研究

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医学信息 2024年第1期37卷 49-53+60页

作者：黄美淳马金辉郭静李俞彤余勇晖海南医学院管理学院海南医学院公共卫生与全健康国际学院

目的了解我国人兽共患病防治领域政策的侧重点和整体布局，分析政策工具的应用情况，为我国人兽共患病防治领域政策的出台和完善提供参考。方法基于政策工具类型和人兽共患病研究热点两个维度构建二维分析框架，采用内容分析法，对34... 详细信息

目的了解我国人兽共患病防治领域政策的侧重点和整体布局，分析政策工具的应用情况，为我国人兽共患病防治领域政策的出台和完善提供参考。方法基于政策工具类型和人兽共患病研究热点两个维度构建二维分析框架，采用内容分析法，对34篇人兽共患病政策文本、293条政策工具进行分析。结果在X维度中，环境型政策使用最多，供给型次之，需求型最少，分别占52.55%、36.19%、11.26%；在Y维度中，防治策略占比最大，疫苗研制次之，临床诊断较低，致病原最少，分别占59.73%、23.21%、11.94%、5.12%；在二维耦合分析中，防治策略在供给型、环境型政策工具应用中均占比最高，需求型政策工具整体使用较少。结论我国人兽共患病防控政策在两个维度的分布均不合理；政策工具的内部和外部结构存在失衡的现象。建议优化政策工具的结构，注重政策工具的使用，提高政策的二维耦合程度，促进人兽共患病防控领域的可持续发展。

关键词：政策工具人兽共患病文本分析

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重庆中心城区轨道与地面公交换乘规划实践

交通企业管理 2024年第5期39卷 71-73页

作者：张菲娜周进均喻永辉重庆市交通规划研究院

随着重庆市主城区现状轨道交通线网规模不断增大,轨道交通机动化出行分担率从2016年的13.3%逐年增加至2021年的20.0%,但公共交通机动化分担率却由59.3%下降至55.3%。轨道和地面公交整体效应未得到充分发挥,轨道与地面公交换乘不便已成... 详细信息

随着重庆市主城区现状轨道交通线网规模不断增大,轨道交通机动化出行分担率从2016年的13.3%逐年增加至2021年的20.0%,但公共交通机动化分担率却由59.3%下降至55.3%。轨道和地面公交整体效应未得到充分发挥,轨道与地面公交换乘不便已成为公共交通系统整体发展的短板。为落实国家、市委市政府“坚持绿色交通发展导向,构建公共交通主导的城市交通出行结构”发展战略和《重庆市城市提升行动计划》实施民生实事项目的要求,全面提升公共交通服务品质和增加市民公共交通出行幸福感,研究围绕老百姓最关心最直接最现实的轨道与地面公交换乘问题开展规划设计工作,也可为其他城市轨道与地面公交换乘规划设计提供经验参考。

关键词：公共交通轨道交通地面公交换乘距离大数据公交首末站

Construction and Expression of Human Survivin and Preparation of Its Polyclonal Antibody

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Chemical Research in Chinese Universities 2008年第6期24卷 767-770页

作者： ZHANG Hai-hong ZHANG Xi-zhen ZHAO Dong-hai SHI He-liang yu yong-hui WU yong-ge yu Xiang-hui KONG Wei Vaccine Research Center College of Life Science Jilin University Changchun 130012 P R. China

Survivin, a novel member of inhibitor of apoptosis（IAP） protein family, is aberrantly expressed in cancer but undetectable in normal, differentiated adult tissues. The cancer-specific expression of survivin, coupled with its importance in inhibiting cell death and in regulating cell division makes it a useful diagnostic marker of cancer and a potential target for cancer treatment. Survivin cDNA amplified from the total RNA of 293 cells through RT-PCR was cloned into prokaryotic expression vector pRSET-B. The recombinant plasmid pRSET-B-Surv was expressed in *** BL21, and the relative molecule mass（Mr） of expressed fusion protein was approximately 21000. The recombinant protein was purified through Ni^2＋ affinity chromatography column and characterized by SDS-PAGE and Western blot. The purified recombinant protein was then injected into rabbits, and antisurvivin polyclonal antibody with a high titer was obtained.

关键词： Clone Survivin Expression Polyclonal antibody

Application Status of Chinese Medicine on Cancer Rehabilitation: A Preliminary Questionnaire Survey

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Chinese Journal of Integrative Medicine 2020年第12期26卷 890-896页

作者： yu hui-yong WANG Xue-qian ZHANG Ying LIU Jie LIN Hong-sheng Department of Respiratory Beijing University of Chinese Medicine Third Affiliated HospitalBeijing(100029)China Department of Oncology Guang’anmen HospitalChina Academy of Chinese Medicine SciencesBeijing(100053)China

Objective:To investigate the application status of Chinese medicine(CM)in cancer rehabilitation,so as to provide references for improving the level of CM cancer ***:A survey questionnaire regarding"application status of CM rehabilitation in cancer patients"for oncologists(doctor version)and cancer patients(patient version)were developed,*** September 2014 to November 2016,a total of 1,000 doctors from oncology department in 48 hospitals and 2,000 cancer patients from CM oncology department from 8 hospitals in China were recruited in this *** psychological,nutrition and exercise rehabilitation guidance for cancer patients provided by doctors,their mastery conditions regarding cancer staging treatment and CM syndrome differentiation,and recommendation from doctors on CM rehabilitation were *** patients’awareness on the importance of psychological,nutrition and exercise rehabilitation,as well as their knowledge and needs for rehabilitation were also *** impact of gender,age,professional title,hospitals grades of physicians on their knowledge of cancer staging treatment and CM syndrome differentiation,and the relationship between gender,age,education level and economic conditions and patient’s knowledge along with the needs of CM rehabilitation were further ***:Totally 1,000 questionnaires were issued to doctors and 963 questionnaires returned,among which 948 were valid representing a response rate of 94.80%.A total of 2,000 questionnaires were issued to patients and 1,705 valid data finally returned with a response rate of 85.25%.The survey showed that cancer patients generally paid much attention to psychological,nutritional and sports rehabilitation,and had a strong demand for CM *** of CM rehabilitation was not well provided by oncologists,and the rehabilitation guidance as well as CM rehabilitation measures were obviously insufficient in cancer *** an

关键词： cancer rehabilitation questionnaire survey Chinese medicine psychological rehabilitation nutrition rehabilitation exercise rehabilitation

Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

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Chinese Journal of Integrative Medicine 2021年第8期27卷 613-620页

作者： yu yong-hui ZHANG Pei WANG Cheng-long LIU Jian-gang LI Peng ZHANG Da-wu Department of Traditional Chinese Medicine China-Japan Friendship HospitalBeijing100029China Graduate Shcool Beijing University of Chinese MedicineBeijing100029China Department of Cardiovascular Disease Mongolian Hospital of Traditional Chinese MedicineWuhaiInner Mongolia Autonomous Region016000China Center for Cardiovascular Diseases Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing100091China

Objective To investigate the effects and underlying mechanisms of Panax quinquefolium saponin(PQS)on energy deficiency in hypoxia-reperfusion(H/R)induced *** The H/R injury involved hypoxia for 3 h and then reperfusion for 2 *** recruited from neonatal rat ventricular myocytes(NRVMs)were randomly divided into control,H/R,H/R+compound C(C.C),H/R+PQS,and H/R+C.C+PQS *** assay,lactase dehydrogenase(LDH)leakage and early apoptosis rate were evaluated to assess cell *** of high energy phosphate compounds were conducted to detect the energy *** expression levels of adenosine monophosphate-activated protein kinase a(AMPKα),glucose transporter 4(GLUT4),phosphate fructose kinase 2(PFK2),fatty acid translocase/cluster of differentiation 36(FAT/CD36),and acetyl CoA carboxylase 2(ACC2)in the regulatory pathways were measured by Western *** staining of GLUT4 and FAT/CD36 was used to observe the mobilization of metabolic *** PQS(50 mg/L)pretreatment significantly alleviated H/R-induced inhibition of NRVMs viability,up-regulation of LDH leakage,acceleration of early apoptosis,and reduction of energy production(P<0.05).Compared with the H/R group,up-regulated expression of AMPKα,GLUT4,PFK2,FAT/CD36 and ACC2 were observed,and more GLUT4 and FAT/CD36 expressions were detected on the membrane in the H/R+PQS group(P<0.05).These effects of PQS on H/R-induced NRVMs were eliminated in the H/R+C.C+PQS group(P<0.05).Conclusion PQS has prominent advantages in protecting NRVMs from H/R-induced cell damages and energy metabolic disorders,by activation of AMPKα-mediated GLUT4-PFK2 and FAT/CD36-ACC2 pathways.

关键词： Panax quinquefolium saponin Chinese medicine neonatal rat ventricular myocytes hypoxia-reperfusion energy metabolism adenosine monophosphate-activated protein kinaseαpathways

Mold breakout prediction based on computer vision and machine learning

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Journal of Iron and Steel Research International 2024年第8期31卷 1947-1959页

作者： Yan-yu Wang Qi-can Wang yong-chang Zhang yong-hui Cheng Man Yao Xu-dong Wang School of Materials Science and Engineering Dalian University of TechnologyDalian 116024LiaoningChina Key Laboratory of Solidification Control and Digital Preparation Technology Dalian University of TechnologyDalian 116024LiaoningChina

Breakout is the most serious production accident in continuous casting and must be detected and predicted by stable and reliable *** sticking region,which forms on the local copper plate and expanded into a"V"shape,is the typical precursor of ***,computer vision technology was exploited to visualize the temperature change rate of the copper plate based on the temperature signals from thermocouples;then,the static and dynamic features of the abnormal sticking region were ***,logistic regression and Adaboost models were used to study and identify these features,resulting in the development of a mold breakout prediction model based on computer vision and machine *** test results demonstrate that the proposed model can effectively distinguish anomalous temperature patterns and considerably reduce false alarms without any missing *** a result,the proposed method could offer valuable insights into the realm of abnormality detection and prediction during continuous casting process.

关键词： Abnormality detection Image processing Adaboost Logistic regression Continuous casting

Ozone decomposition under the irradiation of 253.7 nm in the presence of methyl bromide

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Journal of Environmental Sciences 2000年第3期12卷 266-269页

作者： LI Chao\|min, HOU Jian, yu yong, HOU hui\|qi (Institute of Environmental Science, Fudan University, Shanghai 200433, China) Institute of Environmental Science Fudan University Shanghai 200433 China

In this paper the mechanism and kinetic of ozone decomposition under the irradiation of 253.7 nm UV\|light were studied. The quantum yield of the ozone depletion in the presence of methyl bromide is further determine... 详细信息

关键词： methyl bromide ozone UV

Adhesion property of AlCrNbSiTi high-entropy alloy coating on zirconium:experimental and theoretical studies

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Nuclear Science and Techniques 2024年第8期35卷 79-91页

作者： Bao‑Liang Zhang Wen‑Guan Liu Meng‑He Tu Can Fang Yan Liu yu‑hui Wang yong Hu hui Wang China Institute of Atomic Energy Beijing 102413China Sino‑French Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China School of Power and Mechanical Engineering Wuhan UniversityWuhan 430072China

Experimental scratch tests and first-principles calculations were used to investigate the adhesion property of AlCrNbSiTi high-entropy alloy(HEA)coatings on zirconium *** HEA and Cr coatings were deposited on Zr alloy substrates using multi-arc ion plating technology,and scratch tests were subsequently conducted to estimate the adhesion property of the *** results indicated that Cr coatings had better adhesion strength than HEA coatings,and the HEA coatings showed *** special quasi-random structure approach was used to build HEA models,and Cr/Zr and HEA/Zr interface models were employed to investigate the cohesion between the coatings and Zr substrate using first-principles *** calculated interface energies showed that the cohesion between the Cr coating and the Zr substrate was stronger than that of the HEA coating with *** contrary to Al or Si in the HEA coating,Cr,Nb,and Ti atoms binded strongly with Zr *** on the calculated elastic constants,it was found that low Cr and high Al content decreased the mechanical performances of HEA ***,this study demonstrated the utilization of a combined approach involving first-principles calculations and experimental studies for future HEA coating development.

关键词： High-entropy alloy coating Cr coating Adhesion property Scratch test First-principles calculation