Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

作     者：YU Yong-hui ZHANG Pei WANG Cheng-long LIU Jian-gang LI Peng ZHANG Da-wu YU Yong-hui;ZHANG Pei;WANG Cheng-long;LIU Jian-gang;LI Peng;ZHANG Da-wu

作者机构：Department of Traditional Chinese MedicineChina-Japan Friendship HospitalBeijing100029China Graduate ShcoolBeijing University of Chinese MedicineBeijing100029China Department of Cardiovascular DiseaseMongolian Hospital of Traditional Chinese MedicineWuhaiInner Mongolia Autonomous Region016000China Center for Cardiovascular DiseasesXiyuan HospitalChina Academy of Chinese Medical SciencesBeijing100091China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2021年第27卷第8期

页      面：613-620页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(No.81273934 and No.81874410) 

主　　题：Panax quinquefolium saponin Chinese medicine neonatal rat ventricular myocytes hypoxia-reperfusion energy metabolism adenosine monophosphate-activated protein kinaseαpathways 

摘      要：Objective To investigate the effects and underlying mechanisms of Panax quinquefolium saponin(PQS)on energy deficiency in hypoxia-reperfusion(H/R)induced *** The H/R injury involved hypoxia for 3 h and then reperfusion for 2 *** recruited from neonatal rat ventricular myocytes(NRVMs)were randomly divided into control,H/R,H/R+compound C(C.C),H/R+PQS,and H/R+C.C+PQS *** assay,lactase dehydrogenase(LDH)leakage and early apoptosis rate were evaluated to assess cell *** of high energy phosphate compounds were conducted to detect the energy *** expression levels of adenosine monophosphate-activated protein kinase a(AMPKα),glucose transporter 4(GLUT4),phosphate fructose kinase 2(PFK2),fatty acid translocase/cluster of differentiation 36(FAT/CD36),and acetyl CoA carboxylase 2(ACC2)in the regulatory pathways were measured by Western *** staining of GLUT4 and FAT/CD36 was used to observe the mobilization of metabolic *** PQS(50 mg/L)pretreatment significantly alleviated H/R-induced inhibition of NRVMs viability,up-regulation of LDH leakage,acceleration of early apoptosis,and reduction of energy production(P0.05).Compared with the H/R group,up-regulated expression of AMPKα,GLUT4,PFK2,FAT/CD36 and ACC2 were observed,and more GLUT4 and FAT/CD36 expressions were detected on the membrane in the H/R+PQS group(P0.05).These effects of PQS on H/R-induced NRVMs were eliminated in the H/R+C.C+PQS group(P0.05).Conclusion PQS has prominent advantages in protecting NRVMs from H/R-induced cell damages and energy metabolic disorders,by activation of AMPKα-mediated GLUT4-PFK2 and FAT/CD36-ACC2 pathways.