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Deferoxamine promotes recovery of traumatic spinal cord injury by inhibiting ferroptosis

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Neural Regeneration Research 2019年第3期14卷 532-541页

作者： Xue Yao Yan Zhang Jian Hao Hui-quan Duan Chen-Xi Zhao Chao Sun Bo Li Bao-you fan Xu Wang Wen-xiang Li Xuan-Hao Fu yong Hu Chang Liu xiao-Hong Kong shi-Qing Feng Department of Orthopedics Tianjin Medical University General Hospital State Key Laboratory of Medicinal Chemical Biology Nankai University International Science and Technology Cooperation Base of Spinal Cord Injury Department of Orthopedics Nankai Hospital Department of Orthopedic and Traumatology The University of Hong Kong Pokfulam Hong Kong Special Administrative Region School of Medicine Nankai University Tianjin Neurological Institute Key Laboratory of Post-Neuroinjury Neuro-repair and Regeneration in Central Nervous System Ministry of Education and Tianjin City

Ferroptosis is an iron-dependent novel cell death pathway. Deferoxamine, a ferroptosis inhibitor, has been reported to promote spinal cord injury repair. It has yet to be clarified whether ferroptosis inhibition represents the mechanism of action of Deferoxamine on spinal cord injury recovery. A rat model of Deferoxamine at thoracic 10 segment was established using a modified Allen's method. Ninety 8-week-old female Wistar rats were used. Rats in the Deferoxamine group were intraperitoneally injected with 100 mg/kg Deferoxamine 30 minutes before injury. Simultaneously, the Sham and Deferoxamine groups served as controls. Drug administration was conducted for 7 consecutive days. The results were as follows:(1) Electron microscopy revealed shrunken mitochondria in the spinal cord injury group.(2) The Basso, Beattie and Bresnahan locomotor rating score showed that recovery of the hindlimb was remarkably better in the Deferoxamine group than in the spinal cord injury group.(3) The iron concentration was lower in the Deferoxamine group than in the spinal cord injury group after injury.(4) Western blot assay revealed that, compared with the spinal cord injury group, GPX4, xCT, and glutathione expression was markedly increased in the Deferoxamine group.(5) Real-time polymerase chain reaction revealed that, compared with the Deferoxamine group, mRNA levels of ferroptosis-related genes Acyl-CoA synthetase family member 2(ACSF2) and iron-responsive element-binding protein 2(IREB2) were up-regulated in the Deferoxamine group.(6) Deferoxamine increased survival of neurons and inhibited gliosis. These findings confirm that Deferoxamine can repair spinal cord injury by inhibiting ferroptosis. Targeting ferroptosis is therefore a promising therapeutic approach for spinal cord injury.

关键词： nerve regeneration iron spinal cord injury secondary injury ferroptosis deferoxamine GPX4 xCT treatment astrogliosis lipid peroxidation neural regeneration

Neurofibromatosis type 1 with multiple gastrointestinal stromal tumors:A case report

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World Journal of Clinical Cases 2023年第10期11卷 2336-2342页

作者： Min-quan Yao Yu-Peng Jiang bing-Hong Yi yong Yang da-Zhuang Sun Jin-Xing fan Department of Gastrointestinal Surgery Tongxiang First People’s HospitalJiaxing 314500Zhejiang ProvinceChina Endoscopy Center Tongxiang First People’s HospitalJiaxing 314500Zhejiang ProvinceChina

BACKGROUND Neurofibromatosis type 1(NF1)is characterized by café-au-lait patches on the skin and the presence of *** stromal tumor(GIST)is the most common non-neurological tumor in NF1 *** NF1-associated GIST,KIT and PDGFRA mutations are frequently absent and imatinib is *** resection is first-line *** SUMMARY A 56-year-old woman with NF1 was hospitalized because of an incidental pelvic *** examination was notable for multiple café-au-lait patches and numerous subcutaneous soft nodular masses of the skin of the head,face,trunk,and *** abdomen was soft and *** masses were *** rectal examination was *** computed tomography was suspicious for GIST or solitary fibrous *** was performed,which identified eight well-demarcated masses in the *** were resected and pathologically diagnosed as *** patient was discharged on day 7 after surgery without *** tumor recurrence was evident at the 6-mo *** Laparoscopy is effective for both diagnosis and treatment of NF1-associated GIST.

关键词： Neurofibromatosis type 1 Gastrointestinal stromal tumors KIT PDGFRA Laparoscopy Case report

Simultaneous Screening and Analysis of Anti-inflammatory and Antiproliferative Compounds from Euphorbia maculata Combining Bio-affinity Ultrafiltration with Multiple Drug Targets

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Journal of Analysis and Testing 2022年第2期6卷 98-110页

作者： Tojofaniry Fabien Rakotondrabe Min-Xia fan yong-Li Zhang ming-quan Guo CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China University of Chinese Academy of Sciences Beijing 100049China Sino-Africa Joint Research Center Chinese Academy of SciencesWuhan 430074China Innovation Academy for Drug Discovery and Development Chinese Academy of SciencesShanghai 201203China

Euphorbia maculata has long been used for managing different impairments in Asian ***,its antioxidant,anti-inflammatory and antiproliferative potentialities,along with its potential bioactive compounds remain *** this context,a bio-a ffinity ultrafiltration strategy was developed to fish out ligand candidates against Cycloxygenase-2(COX-2),Topoisomerase I(Topo I),and TopoisomeraseⅡ(TopoⅡ).Thereafter,lead compounds activities were assessed in silico and in vitro for ascertaining the screening results and forecasting its corresponding *** a result,the *** ethyl acetate(EMEA)fraction showed interesting COX-2 inhibition activity with an IC 50 value of 0.67±0.09μg/mL,as well as good growth inhibitions for three malignant cell *** chemical fingerprinting was also conducted to enable a tentative identification of 17 compounds,among which,11 were assessed as ligand candidates to COX-2,8 compounds to Topo I,and 10 compounds to TopoⅡ.Dihydromyricetin and quercetin-3-O-arabinoside were revealed to be multipotent compounds which exerted good a ffinity to the three targeted enzymes,and also supported with their molecular docking simulations and in vitro assay *** interrelationship between ***’s associated activities(antioxidant,anti-inflammatory and antiproliferative)was revealed with the corresponding multipotent phytochemical active components from this *** also provided a useful direction for its empirical traditional use and further explorations in the near future.

关键词： Euphorbia maculata Antioxidant Anti-infl ammatory Antiproliferative UF-UPLC-QTOF-MS/MS Multipotent compounds

A simulation study of a windowless gas-stripping room in an E//B neutral particle analyzer

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Nuclear Science and Techniques 2021年第7期32卷 22-30页

作者： Yuan Luo Wei-Ping Lin Pei-Pei Ren Guo-Feng Qu Jing-Jun Zhu Xing-quan Liu xiao-bing Luo Zhu An Roy Wada Lin-Ge Zang Yu-fan Qu Zhong-bing shi Key Laboratory of Radiation Physics and Technology of the Ministry of Education Institute of Nuclear Science and TechnologySichuan UniversityChengdu 610064China Cyclotron Institute Texas A&M UniversityCollege StationTX 77843USA School of Physics Henan Normal UniversityXinxiang 453007China Southwestern Institute of Physics Chengdu 610064China

The neutral particle analyzer(NPA)is one of the crucial diagnostic devices in a Tokamak *** stripping unit is one of the main parts of the NPA.A windowless gas-stripping room with two differential pipes has been constructed in a parallel electric and magnetic fields(E//B)*** pressure distributions in the stripping chamber are simulated by ANSYS Fluent together with MolFlow+.Based on the pressure distributions obtained from the simulation,the stripping efficiency of the E//B NPA is studied using *** reaction physics is modified to track the charge state of each particle in a cross-section-based method in *** transmission rates(R)and stripping efficiencies f_(+1)are examined for particle energies ranging from 20 to 200 keV with the input pressure(P_(0)),ranging from 20 to 400 *** to the combined global efficiency,R×f_(+1),P_(0)=240 Pa is obtained as the optimum pressure for the maximum global efficiency in the incident energy range investigated.

关键词： Neutral particle analyzer Windowless gas-stripping chamber Stripping efficiency Ansys Fluent MolFlow+ GEANT4

Elongational Flow-induced Crystallization of Poly(L-lactic acid)Telechelic Ionomers

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Chinese Journal of Polymer Science 2024年第12期42卷 1957-1965,I0010页

作者： fan Liu Shao-yong Huang Jian Tang quan Chen State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China

In this study,we prepared unentangled and slightly entangled poly(L-lactic acid)telechelic ionomer samples(Mn=5 and 16 kg/mol)based on sodium sulfonate *** telechelic samples exhibit extremely slow crystallization kinetics below the melting temperature T_(m) and above the glass transition temperature T_(g),which enables us to examine the linear viscoelasticity of the ionomer melt samples *** application of either the shear flow(at 85℃)or elongational flow(between 70 and 90℃)strongly accelerates the crystallization,leading to strong strain hardening and formation of highly orientedα*** on the relative average rates of the strain-induced dissociation and strain-induced crystallization,the stress evolution can be classified into two cases,and the critical work for strain-induced crystallization is higher in case where the strain-induced dissociation occurs earlier than the strain-induced crystallization.

关键词： Polylactides telechelic ionomers Flow-induced crystallization Strain-induced dissociation

Flash flood hazard mapping: A pilot case study in Xiapu River Basin, China

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Water Science and Engineering 2015年第3期8卷 195-204页

作者： da-wei Zhang Jin quan Hong-bin Zhang fan Wang Hong Wang xiao-yan He China Institute of Water Resources and Hydropower Research Research Center on Flood and Drought Disaster Reduction Ministry of Water Resources

Flash flood hazard mapping is a supporting component of non-structural measures for flash flood prevention. Pilot case studies are necessary to develop more practicable methods for the technical support systems of flash flood hazard mapping. In this study, the headwater catchment of the Xiapu River Basin in central China was selected as a pilot study area for flash flood hazard mapping. A conceptual distributed hydrological model was developed for flood calculation based on the framework of the Xinanjiang model, which is widely used in humid and semi-humid regions in China. The developed model employs the geomorphological unit hydrograph method, which is extremely valuable when simulating the overland flow process in ungauged catchments, as compared with the original Xinanjiang model. The model was tested in the pilot study area, and the results agree with the measured data on the whole. After calibration and validation, the model is shown to be a useful tool for flash flood calculation. A practicable method for flash flood hazard mapping using the calculated peak discharge and digital elevation model data was presented, and three levels of flood hazards were classified. The resulting flash flood hazard maps indicate that the method successfully predicts the spatial distribution of flash flood hazards, and it can meet the current requirements in China.

关键词： Flash flood hazard mapping Conceptual distributed hydrological model Xinanjiang model Geomorphological unit hydrograph Xiapu River Basin

Synthesis and Crystal Structure of a Dinuclear Cu(Ⅱ) Complex Based on a Carboxyl-substituted 1H-1,2,3-Triazole and Its DNA Cleavage Activity

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Chinese Journal of Structural Chemistry 2015年第6期34卷 917-924页

作者： LIU Wen-Qian ZHOU shi-Lei fan ming-Zhu PAN Zhi-quan CHEN Yun-Feng School of Chemistry and Environmental Engineering Wuhan Institute of Technology

The novel dinuclear copper complex [Cu2（H20）2（DMF）2（L）2] （1, H2L = 5-phenyl- 2H-1,2,3-triazole-4-carboxylic acid, DMF = N,N-dimethyl-formamide） has been synthesized and characterized by X-ray single-crystal diffraction. The compound crystallizes in triclinic system, space group P1 with a = 9.591, b = 10.508, c = 15.515A,β = 75.11°, V= 1446.2 A3, Z= 2, Mr = 683.62, Dc = 1.570 g/cm3, μ = 1.531 mm^-1, F（000） = 700, the final R = 0.0404 and wR = 0.1130 for 5327 observed reflections with I 〉 2σ（I）. In each unit of the complex, two Cu2＋ ions coordinated with two triazole ligands to form a dimeric [5,6,5] tricyclic structure. The central Cu atom is five-coordinated, and each copper atom shows a square pyramidal geometry. The crystal structure is stabilized by the inversion-related O-H…O hydrogen bond and C-H…O hydrogen bonding interactions to form a layer structure. Fluorescent spectra show an obvious quenching of fluorescence compared with free 1,2,3-triazole ligand. The results of agarose gel electrophoresis indicate that this complex can cleave the plasmid supercoiled DNA within shorter time in the 50-folds excess of ascorbate under physiological conditions, providing a new example in the research for artificial metal nucleic acid enzyme.

关键词： 1,2,3-triazole copper complex crystal structure fluorescent property DNA cleavage activity

Expression and function of classical protein kinase C isoenzymes in gastric cancer cell line and its drugresistant sublines

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World Journal of Gastroenterology 2002年第3期8卷 441-445页

作者： Ying han Zhe-Yi han Xin-Min Zhou Ru shi Yue Zheng yong-quan shi Ji-Yan Miao Bo-Rong Pan dai-ming fan Institute of Digestive Disease Xijing HospitalFourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina Department of Pharmaeology and Reagents Xijing HospitalFourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina the 6th Undergraduate Section Fourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina Oncology Center of Xijing Hospital Fourth Military Medical UniversityXi’an 710032Shannxi ProvinceChina

AIM: To investigate the expression and function of classicalprotein kinase C (PKC) isoenzymes in inducing MDRphenotype in gastric cancer ***: Two cell lines were used in the study: gastriccancer cell SGC7901 and its drug-resistant cell SGC7901/VCRstepwise-selected by vincristine 0.3, 0. 7 and 1.0 mg@ L-1 ,respectively. The expression of classical PKC (cPKC)isoenzymes in SGC7901 cells and SGC7901/VCR cells weredetected using immunofluorescent cytochemistry, laserconfocal scanning microscope and Wsstern blot. The effectsof anti-PKC isoenzymes antibody of adriamycinaccumulation in SGC7901/VCR cells were determined usingflow cytometric ***: (1) SGC7901 cells exhibited positive staining ofPKC-α. SGC7901/VCR cells exhibited stronger staining ofPKC-α than SGC7901 cells. The higher dosage vincristineselected, the much stronger staining of PKC-α was observedon SGC7901/VCR cells. (2) Both SGC7901 and SGC7901/VCRcells exhibited positive staining of PKC-βⅠ and PKC-βⅡ withno significant difference. ( 3 ) Compared with SGC7901,SGC7901/VCR cells had decreased adriamycin accumulationand retention. Accumulation of adriamycin in SGC7901 was5.21 + 2.56 mg@ L-1, in SGC7901/VCR 0.3 was 0.85 + 0.29 mg@L-1 , in SGC7901/VCR 0.7 was 0.81 + 0.32 og@ L-1 , and inSGC7901NCR 1.0 was 0.80 + 0.33 mg @ L-1; Retention ofadriamycin in SGC 7901 was 2.51 + 1.23 mg@L-1, in SGC7901/VCR 0.3 was 0.47 + 0.14 mg@ L-1 , in SGC7901/VCR 0.7 was 0.44 + 0.15 mg@ L-1, and in SGC 7901/VCR 1.0 was 0.41 + 0.1 1mg @ L-1 . (4) Fluorescence intensity presented adriamycinaccumulation in SGC7901/VCR cells was increased from 1.14+0.36 to 2.71 +0.94 when cells were co-incubated with anti-PKC-αbut not with anti-PKC-βⅠ, PKC-βⅡ and PKCγ ***: PKC-α, but not PKC-βⅠ, PKC-βⅡ or PKCγ,may play a role in multidrug resistance of gastric cancercells SGC7901/VCR.

关键词： C蛋白激酶同工酶胃癌细胞表达抗药性

Baseline optimization for scalar magnetometer array and its application in magnetic target localization

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Chinese Physics B 2018年第6期27卷 213-219页

作者： Li-ming fan quan Zheng Xi-Yuan Kang xiao-Jun Zhang Chong Kang College of Science Harbin Engineering University Harbin 150001 China College of Mechanical and Electrical Engineering Harbin Engineering University Harbin 150001 China College of Computer Science and Technology Harbin Engineering University Harbin 150001 China

Generally, a magnetic target can be described with six parameters, three describing the position and three describing the magnetic moment. Due to a lack of sufficient components from one magnetometer, we need more than one magnetometer when locating the magnetic target. Thus, a magnetometer array should be designed. The baseline of the array is an important factor that affects the localization accuracy of the target. In this paper, we focus on the localization of a static target by using a scalar magnetometer array. We present the scalar magnetometer array with a cross-shaped *** propose a method of determining the optimal baseline according to the parameters of the magnetometer and detection requirements. In the method, we use the traditional signal-to-noise ratio（SNR） as a performance index, and obtain the optimal baseline of the array by using the Monte Carlo method. The proposed method of determining the optimal baseline is verified in simulation. The arrays with different baselines are used to locate a static magnetic target. The results show that the location performance is better when using the array with the optimal baseline determined by the proposed method.

关键词： scalar magnetometer array baseline optimization Monte Carlo simulation magnetic anomaly