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Application of Optical Tweezers in the Research of Molecular Interaction between Lymphocyte Function Associated Antigen-1 and Its Monoclonal Antibody

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Cellular & Molecular Immunology 2007年第3期4卷 221-225页

作者： Haodong Chen Kuikui Ge yinmei li Jianguang Wu Yongqiang Gu Haiming Wei Zhigang Tian Institute of Immunology Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei 230027 China Hefei National Laboratory for Physical Sciences at Microscale and Physics Department University of Science and Technology of China Hefei 230027 China

Lymphocyte function associated antigen-1 （CD11a/CD18, LFA-1） plays an important role in the structure of the immunological synapse and is required for efficient lysis of cytotoxic T lymphocytes （CTLs） and natural killer （NK） cells. To study the activation mode of LFA-1 on the NK cell surface, optical tweezers were used in the work. As an emerging technology, optical tweezers are widely used to manipulate microscopic objects and measure the forces of molecular interactions in the field of biological research. In our study, a new platform was constructed to study the single molecular behavior of receptor on cell surface using optical tweezers. Based on the platform, the interaction between an NK cell and a polystyrene microsphere coated with anti-LFA-1 antibody was observed. The result confirmed that the adhesion forces between an NK cell and a polystyrene bead were time-dependent. According to our findings, we propose that anti-LFA-1 antibody may cause the clustering of LFA-1 on NK cell surface. Cellular ＆ Molecular Immunology.

关键词： NK cell LFA- 1 optical tweezers polystyrene microsphere

Application of BP neural networks in non-linearity correction of optical tweezers

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Frontiers of Electrical and Electronic Engineering in China 2008年第4期3卷 475-479页

作者： Ziqiang WANG yinmei li liren LOU Henghua WEI Zhong WANG School of Science University of Science and Technology of ChinaHefei 230026China

The back-propagation(BP)neural network is proposed to correct nonlinearity and optimize the force measurement and calibration of an optical tweezer *** the low convergence rate of the BP algo-rithm,the Levenberg-Marquardt(LM)algorithm is used to improve the BP *** proposed method is experimentally studied for force calibration in a typical optical tweezer system using *** result shows that with the nonlinear correction using BP net-works,the range of force measurement of an optical tweezer system is enlarged by 30%and the precision is also improved compared with the polynomial fitting *** is demonstrated that nonlinear correction by the neural network method effectively improves the per-formance of optical tweezers without adding or changing the measuring system.

关键词： optical tweezers back-propagation(BP) nonlinearity correction

Aurora A orchestrates entosis by regulating a dynamic MCAK-TIP150 interaction

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Journal of Molecular Cell Biology 2014年第3期8卷 240-254页

作者： PengXia JinhuaZhou Xiaoyu Song BingWu Xingliu Di li Shuyuan Zhang Zhikai Wang H uijuan Yu Tarsha Ward Jiancun Zhang yinmei li Xiaoning Wang YongChen Zhen Guo Xuebiao Yao Anhui Key Laboratory of Cellular Dynamics & Chemical Biology Department of Optics and Optical Engineering and Hefei National Laboratory for Physical Sciences at Nanoscale University of Science and Technology of China Hefei 230027 China Molecular Imaging Center Atlanta Clinical and Translational Science Institute Morehouse School of Medicine Atlanta GA 30310 USA Harvard Medical School Boston MA 02115 USA Guangzhou Institutes of Biomedicine and Health Guangzhou 510513 China The 301 Hospital Beijing 100039 China Xijing Hospital The Fourth Military Medical University Xi'an 710032 China

Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto the microtubule plus ends and orchestrates micro- tubule plus-end dynamics during cell division. Here we show that TIP150 cooperates with MCAK to govern entosis via a regulatory cir- cuitry that involves Aurora A-mediated phosphorylation of MCAK. Our biochemical analyses show that MCAK forms an intra-molecular association, which is essential for TIP150 binding. Interestingly, Aurora A-mediated phosphorylation of MCAK modulates its intra-mo- lecular association, which perturbs the MCAK-TI P150 interaction in vitro and inhibits entosis in vivo. To probe if MCAK-TIP150 inter- action regulates microtubule plasticity to affect the mechanical properties of ceUs during entosis, we used an optical trap to measure the mechanical rigidity of live MCF7 ceils. We find that the MCAK cooperates with TIP150 to promote microtubule dynamics and modulate the mechanical rigidity of the cells during entosis. Our results show that a dynamic interaction of MCAK-TI P150 orchestrated by Aurora A-mediated phosphorylation governs entosis via regulating microtubule plus-end dynamics and cell rigidity. These data reveal a previously unknown mechanism of Aurora A regulation in the control of microtubule plasticity during ceU-in-ceU pro- cesses.

关键词： Aurora A TIP150 MCAK entosis microtubule plus-end kinesin

Dynamic 3D holographic projection of vectorial images with a multimode fiber

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Photonics Research 2024年第6期12卷 1344-1350页

作者： JINGHAN ZHUANG PANPAN YU YIFAN liU YIJING WU ZIQIANG WANG yinmei li LEI GONG Department of Optics and Optical Engineering University of Science and Technology of ChinaHefei 230026China Advanced Laser Technology Laboratory of Anhui Province Hefei 230026China

An optical multimode fiber(MMF)is capable of delivering structured light modes or complex images with high ***,we present a holographic approach to enable the MMF as a 3D holographic projector with the capability of complete polarization *** harnessing the strong coupling of the spatial and polarization degrees of freedom of light propagating through MMFs,our approach realizes active control of the output intensity and polarization in 3D space by shaping only the wavefront of the incident *** this manner,we demonstrate MMF-based holographic projection of vectorial images on multiple planes via a phase-only ***,dynamic projection of polarization-multiplexed grayscale images is presented with an averaged Pearson correlation coefficient of up to *** work is expected to benefit fiber-based holographic displays,data transmission,optical imaging,and manipulation.

关键词： holographic projection enable

汉英主题结构的典型特征束:基于口语语料库的话语分析

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外语与外语教学 2020年第4期 124-137,F0003页

作者：李银美袁凤识北方工业大学文法学院北京100144

主题结构的研究成果丰富,但不同结构在汉英语言中的话语表现尚存明显分歧。本文构建了一个主题原型范畴考察的典型特征束,以汉英随意性自然口语对话为语料,分析汉英四类主题结构的使用倾向。研究发现,汉语主题结构使用频率较高,不同结... 详细信息

主题结构的研究成果丰富,但不同结构在汉英语言中的话语表现尚存明显分歧。本文构建了一个主题原型范畴考察的典型特征束,以汉英随意性自然口语对话为语料,分析汉英四类主题结构的使用倾向。研究发现,汉语主题结构使用频率较高,不同结构在主题指称义和回指性上表现分立,但均易成为后续话语的凸显主题,具有较高的预指性,汉语主题的话语属性突出;英语主题结构使用频率较低,不同结构在主题指称义和回指性上表现接近,但在预指性上表现不同,英语主题的语法特征更为突出。主题结构的原型分析表明主题结构间不具有完全的替换关系。

关键词：主题结构典型特征束有定性回指性预指性

情境植入及其层级限定:认知功能路向的语句标记研究

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外语教学 2023年第3期44卷 30-36页

作者：李银美王义娜北方工业大学文法学院北京航空航天大学外国语学院

如何标示所述事件的认识地位是定式小句研究的重要议题,以Langacker和Nuyts为代表的认知功能路向从小句的情境植入和事态限定层级角度,推进了对时、体、情态和言据等四大语义范畴的语句标记认识。梳理其理论观点和研究进展的进步意义有... 详细信息

如何标示所述事件的认识地位是定式小句研究的重要议题,以Langacker和Nuyts为代表的认知功能路向从小句的情境植入和事态限定层级角度,推进了对时、体、情态和言据等四大语义范畴的语句标记认识。梳理其理论观点和研究进展的进步意义有三:1)情境植入关注言者如何使用语言标记植入不同的认知场景,从言者介入角度探究语句标记,推动了语言研究的认识转向;2)基于事件的现实性和命题事件的来源可信度,提出了小句情境植入的句内/句外标记层级,将情境植入方式由时态、情态扩展到言据标记,言据在小句情境植入中的作用受到了重视;3)从层级限定看,形态、词汇及句法手段都可发挥语句标记作用,开辟了一条语法标记研究的新路径。

关键词：情境植入层级事态限定时态情态言据性

汉英主题结构的标记性:基于口语语料库的话语认知分析

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外国语 2016年第6期39卷 34-45页

作者：王义娜李银美北京航空航天大学外国语学院北京100191

以往主题结构的标记性研究主要关注其主题表现。本文基于认知语法的当前话语空间分析模型,以日常对话为语料,从主题和述题的情境植入角度就三类主题结构在句法、话语和认知层面上的标记性等级进行了汉英对比考察。研究发现:汉语的主题... 详细信息

以往主题结构的标记性研究主要关注其主题表现。本文基于认知语法的当前话语空间分析模型,以日常对话为语料,从主题和述题的情境植入角度就三类主题结构在句法、话语和认知层面上的标记性等级进行了汉英对比考察。研究发现:汉语的主题标记性低于英语,而述题表现却相反;就结构间对比:汉英偏置结构的典型特征分布基本一致,但在两类前置结构上差异显著。其标记性倾向受到认知情境因素制约:汉语主题的情境植入方式简单,以延续主题和提升主题为主,话语凸显度较高,易使用主观评价类述题,而英语主题情境植入方式复杂,多为提升或旁枝主题,更易与客观描写述题搭建语义关联。不同主题结构的标记性倾向可反观汉英语言的类型差异。

关键词：主题结构情境植入标记性典型特征束当前话语空间

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汉英主题结构的主观性:述题的情境植入视角

外语教学与研究 2019年第2期51卷 189-201,319页

作者：王义娜李银美北京航空航天大学北方工业大学

本文基于认知语法的情境植入视角,从述题角度对比考察汉英主题结构在自然口语中的使用倾向。研究发现,汉英述题小句的情境植入层级差异显著:汉语主题结构偏于态度限定,呈现出明显的主观性特征;而英语主题结构偏好于时间情境限定,主观表... 详细信息

本文基于认知语法的情境植入视角,从述题角度对比考察汉英主题结构在自然口语中的使用倾向。研究发现,汉英述题小句的情境植入层级差异显著:汉语主题结构偏于态度限定,呈现出明显的主观性特征;而英语主题结构偏好于时间情境限定,主观表达受限。从具体对比数据可见:汉语的悬置主题结构最为主观,其次是主语主题和偏置主题,宾语主题结构相对客观;而英语主要为偏置主题和主语主题结构,前者汉英表现趋同,后者差异明显。从限定方式看,汉语的高层情境标记具有事态限定和主题关涉双重作用,其表达偏好可为汉英语言的类型差异提供新的解释角度。

关键词：主题结构述题小句主观性情境植入事态限定

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双光镊测量胶体微粒间相互作用势

中国激光 2008年第1期35卷 151-155页

作者：任洪亮庄礼辉李银妹中国科学技术大学物理系合肥微尺度物质科学国家实验室安徽合肥230026

分析了两个存在相互作用势的胶体微粒的布朗运动,讨论了通过微粒布朗运动的显微观测来测量胶体微粒间相互作用势的实验方法。将双光镊系统与同步斩光器相结合,建立了相应的测量系统。对1μm直径的聚苯乙烯球悬浮液中两个小球之间的静电... 详细信息

分析了两个存在相互作用势的胶体微粒的布朗运动,讨论了通过微粒布朗运动的显微观测来测量胶体微粒间相互作用势的实验方法。将双光镊系统与同步斩光器相结合,建立了相应的测量系统。对1μm直径的聚苯乙烯球悬浮液中两个小球之间的静电相互作用势进行了实验研究。实验结果和DLVO(Derjagin-Landau-Verwey-Overbeek)理论符合较好,为DLVO理论提供了直接的微观实验依据。对决定实验精度和效率的一些实验因素,包括测量过程中光镊的开关切换时间、光镊打开和关闭的持续时间、样品离样品池底面高度和实验测量区间划分精度等进行了具体讨论。所建立的实验装置和方法可用于测量不同类型胶体粒子间的相互作用势,这为深入研究决定分散体系宏观性质的微观基础提供了一种有效的手段。

关键词：测量相互作用势双光镊斩光器