检索结果-南通市图书馆

维普期刊数据库博看期刊

Autophagy plays a protective role in advanced glycation end products-induced apoptosis of chondrocytes via regulation of tumor necrosis factor-α,nuclear factor-κ B and reactive oxygen species

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2018年第1期11卷 73-77页

作者： Zhi-Jiang Sun Ya-yi xia Orthopaedics Key Laboratory of Gansu Province The Second Hospital of Lanzhou University

Objective: To study the adverse effects of advanced glycation end products(AGEs) on chondrocytes and the role of autophagy in this process. Methods: Chondrocytes were harvested from the human articular cartilage tissues in surgery. AGEs were administered during chondrocytes culture. The rapamycin was used to induce autophagy. The cell viability was determined by 3-[4,5-dimethylthiazol2-yl]-2,5-diphenyl tetrazolium bromide(MTT) *** expression of tumor necrosis factor-α(TNF-α) and nuclear factor-κ B(NF-κ B) was detected by quantitative real-time polymerase chain reaction. The reactive oxygen species(ROS) production and apoptosis of the chondrocytes were determined by fluorescent probe and flow cytometer, respectively. Results: The chondrocytes viability was significantly reduced after 12 h incubation with AGEs(P<0.01)). In contrast, rapamycin pretreatment increased the chondrocytes viability through autophagy. AGEs increased TNF-α and NF-κ B mRNA expression of chondrocytes and autophagy receded or proceeded the change. AGEs increased intracellular ROS accumulation and autophagy reversed the change. AGEs accelerated chondrocytes apoptosis and autophagy suspended apoptosis. Conclusions: Accumulation of AGEs may have an adverse role for chondrocytes by increasing TNF-α and NF-κB expression, ROS accumulation and apoptosis; meanwhile, autophagy ameliorates the AGEsinduced adverse effects.

关键词： Advanced glycation end products Autophagy Tumor necrosis factor-α Nuclear factor-κ B Reactive oxygen species Apoptosis Chondrocytes

Switching Behavior Induced by Electric and Magnetic Fields in （La0.73Bi0.27）0.67Ca0.33MnO3

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics Letters 2014年第4期31卷 159-162页

作者： WANG Ye-Shuai xia Nian-Ming ZUO Hua-Kun SHEN yi-Ning xia Zheng-Cai Wuhan National High Magnetic Field Center Huazhong University of Science and Technology Wuhan 430074

Both electric and magnetic field-induced switching behaviors between a high resistive state and a low resistive one are observed in （La0.73Bi0.27）0.67Ca0.33MnO3. The effects of magnetoresistance *** electric-resistance suggest that the applied electric field and magnetic field greatly tune the percolative paths in the phase-separated system. According to the experimental results, the switching behaviors may come from the coexistence of the charge ordering state, and localized and freedom ferromagnetic states, in which the external field destroys partially the localized ferromagnetic states and charge ordering leads to the ferromagnetic state growth, which causes a switch between a high resistive state and low resistive one. This makes the doped manganite a good system for both electric and magnetic field sensor materials.

关键词： (La0._(73)Bi0._(27))_(0.67)Ca_(0.33)MnO_3 Switching applied

miR-451a和miR-21-5p双重实时荧光定量PCR检测体系的建立及应用

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生物化学与生物物理进展 2024年第3期51卷 706-715页

作者：胡舒啸陈惠香胡胜赵一霞季安全李洋廉洁孙启凡中国人民公安大学侦查学院北京100038 公安部鉴定中心现场物证溯源技术国家工程实验室法医遗传学公安部重点实验室北京100038 山西医科大学法医学院太原030001

目的为了提高微量样本中miRNA的检测通量和检测效率,本文建立了一种能够同时准确定量两种miRNA的双重实时荧光定量PCR(real time fluorogenic quantitative PCR)检测体系,并通过实际样本检测验证其应用于体液鉴别的效果。方法设计适用于... 详细信息

目的为了提高微量样本中miRNA的检测通量和检测效率,本文建立了一种能够同时准确定量两种miRNA的双重实时荧光定量PCR(real time fluorogenic quantitative PCR)检测体系,并通过实际样本检测验证其应用于体液鉴别的效果。方法设计适用于miRNA双重检验的相关引物及探针并优化实验体系组分,建立基于TaqMan技术的miRNA双重实时荧光定量PCR检测体系并验证其特异性、灵敏度和可重复性;使用此检测体系对58份不同体液样本中的miR-451a与miR-21-5p进行检测,并借助miR-451a与miR-21-5p的比值鉴定法评估该体系的体液鉴别能力;使用该检测体系样本数据确定的最佳截断值对模拟案件样本进行鉴别。结果优化的检测体系能够实现对血液与非血液、月经血与外周血的100%区分,同时可以实现对模拟案件样本的准确鉴别。结论该双重实时荧光定量PCR检测体系将时间和材料成本均缩短至原来的一半,为后续建立更多重的实时荧光定量PCR检测体系并应用于体液鉴别打下了基础。

关键词：法医遗传学体液鉴别双重实时荧光定量PCR microRNA

Analysis and discussion of a dead-zone protection mal-operation case in Brazil

在线全文

学校读者我要写书评

暂无评论

Journal of Modern Power Systems and Clean Energy 2014年第1期2卷 87-91页

作者： Dehui CHEN yi xia Wei LIU Salvatore MANTUANO NARI Technology Co. LTDNanjing 210003China State Grid Brazil Holding S.A. Rio de Janeiro 20071-004Brazil

A mal-operation case of busbar protection caused by a dead-zone protection abnormality in the Brazilian Midwest Grid on September 19th,2012,is briefly *** operation process of pilot distance,automatic re-closure,busbar differential and dead-zone protection,and the emphasis on the logic scheme of dead-zone protection are *** time delay between the breaker’s main pole and auxiliary contact during re-closure was the main cause in this case,and a defect in the logic allowed it to *** differences in dead-zone protection logic between Brazil and China are discussed.A test platform was constructed in the laboratory,and a site simulated experiment was also *** causes are suggested and test activities are carried out to verify *** show the values of practical engineering approaches to the *** also contributed to avoiding future mal-operations and strengthening the reliability of the protection system,and thus of the power supply in Brazil.

关键词： Brazilian power system Power system Relay protection device Dead-zone protection

Scale-invariant machine-learning model accelerates the discovery of quaternary chalcogenides with ultralow lattice thermal conductivity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

npj Computational Materials 2022年第1期8卷 455-466页

作者： Koushik Pal Cheol Woo Park yi xia Jiahong Shen Chris Wolverton Department of Materials Science and Engineering Northwestern UniversityEvanstonIL60208USA

We design an advanced machine-learning(ML)model based on crystal graph convolutional neural network that is insensitive to volumes(i.e.,scale)of the input crystal structures to discover novel quaternary chalcogenides,AMM′Q3(A/M/M'=alkali,alkaline earth,post-transition metals,lanthanides,and Q=chalcogens).These compounds are shown to possess ultralow lattice thermal conductivity(κ_(l)),a desired requirement for thermal-barrier coatings and *** screening the thermodynamic stability of~1 million compounds using the ML model iteratively and performing density-functional theory(DFT)calculations for a small fraction of compounds,we discover 99 compounds that are validated to be stable in *** several DFT-stable compounds,we calculate theirκl using Peierls–Boltzmann transport equation,which reveals ultralowκ_(l)(<2 Wm^(−1)K^(−1)at room temperature)due to their soft elasticity and strong phonon *** work demonstrates the high efficiency of scale-invariant ML model in predicting novel compounds and presents experimental-research opportunities with these new compounds.

关键词： ultralow thermal invariant

A new strategy for full-length Ebola virus glycoprotein expression in ***

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2016年第6期31卷 500-508页

作者： Junjie Zai yinhua yi Han xia Bo Zhang Zhiming Yuan Key Laboratory of Agricultural and Environmental Microbiology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China University of Chinese Academy of Sciences Beijing 100049 China Key Laboratory of Special Pathogens and Biosafety Center for Emerging Infectious Diseases WuhanInstitute of Virology Chinese Academy of Sciences Wuhan 430071 China

Ebola virus(EBOV) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. Glycoprotein(GP) is the only envelope protein of EBOV, which may play a critical role in virus attachment and entry as well as stimulating host protective immune ***, the lack of expression of full-length GP in Escherichia coli hinders the further study of its function in viral pathogenesis. In this study, the vp40 gene was fused to the full-length gp gene and cloned into a prokaryotic expression vector. We showed that the VP40-GP and GP-VP40 fusion proteins could be expressed in *** at 16 ℃. In addition, it was shown that the position of vp40 in the fusion proteins affected the yields of the fusion proteins, with a higher level of production of the fusion protein when vp40 was upstream of gp compared to when it was downstream. The results provide a strategy for the expression of a large quantity of EBOV full-length GP, which is of importance for further analyzing the relationship between the structure and function of GP and developing an antibody for the treatment of EBOV infection.

关键词： Ebola virus (EBOV) glycoprotein (GP) E.coli prokaryotic expression

Factors influencing physician's behavioral intention to use Traditional Chinese Medicine to treat coronavirus disease 2019 based on the theory of planned behavior

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2022年第4期42卷 633-640页

作者： CHEN Huang SHI Lushaobo SHI Zengping xia yi WANG Dong General Office the People’s Government of Guangdong ProvinceGuangzhou 510045China the Public Health Service System Construction Research Foundation of Guangzhou 510515 China School of Health Management Southern Medical UniversityGuangzhou 510515China the Public Health Service System Construction Research Foundation of Guangzhou Guangzhou 510515China

OBJECTIVE:To explore the factors influencing physicians’intentions to use Traditional Chinese Medicine(TCM)to treat coronavirus disease 2019(COVID-19).METHODS:A cross-sectional,self-report online survey was conducted from March 16,2020,to April 2,2020,in *** were recruited through convenience and snowball *** were collected by using a self-designed questionnaire based on the Theory of Planned *** equation modeling was used for data ***:A total of 494 physicians were enrolled in this ***,the model explained 75.4%and 75.5%of the total variance in intention and attitude,***,attitudes(β=0.467,P<0.001),past behavior(β=0.384,P<0.05),subjective norms(SN)(β=0.177,P<0.001),and perceived behavioral control(PBC)(β=0.133,P<0.05)significantly affected physicians’intention to use ***(β=0.606,P<0.001)and PBC(β=0.569,P<0.01)significantly influenced physicians’attitudes toward using ***(β=0.064,P=0.263)was not a factor affecting ***:Physicians’intention to use TCM was significantly associated with attitude,past behavior,PBC,and *** findings may not only be useful for understanding the influencing factors and paths of physicians’intention to use TCM to treat COVID-19 but also provide a reference for health authorities and policymakers to promote physicians to utilize TCM.

关键词： COVID-19 Medicine,Chinese Traditional physicians intention behavior control

Synthesis and anti-HBV activity of S-substituted 7-mercapto-4-methylcoumarin analogs

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2008年第8期19卷 925-927页

作者： ying Chen Jin Zhou Hai Wang yi xia Zheng Yu Yang Peng xia Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China

Twelve S-substituted 7-mercapto-4-methylcoumarin analogs were synthesized and evaluated for the inhibition to HBV in HepG2 2.***.1.5 cell. Among them, ten compounds exhibited potent inhibition to HBsAg and/or HBeAg with the IC50 values of sub μmol/L level. The ICso of anti-HBsAg activities of 5c and 5j reached 0.01 μmol/L respectively which were 16 fold more potent than that of 3TC. Compounds 3, 5e, 5g, 5h and 5i showed admirable inhibitory activity to both HBsAg and HBeAg. The bioassay results indicated the S-substituted 7-mercapto-4-methylcoumarin analogs merit attention as novel anti-HBV agents.

关键词： Synthesis S-Substituted 7-mercapto-4-methylcoumarin Anti-HBV

在线全文

学校读者我要写书评

暂无评论

基于改进Fi-GNN模型的点击率预测方法

计算机工程与设计 2024年第6期45卷 1720-1727页

作者：夏义春李汪根李豆豆高坤束阳安徽师范大学计算机与信息学院安徽芜湖241002

为解决基线模型(Fi-GNN)特征交互模块设计不合理的问题,提出一种基于改进Fi-GNN模型的点击率预测方法(Fi-GNN-V2)。针对特征交互模块的邻接矩阵没有考虑到异构节点间的多元关系,在计算异构节点间相互作用的权重时增加边类型的嵌入向量,... 详细信息

为解决基线模型(Fi-GNN)特征交互模块设计不合理的问题,提出一种基于改进Fi-GNN模型的点击率预测方法(Fi-GNN-V2)。针对特征交互模块的邻接矩阵没有考虑到异构节点间的多元关系,在计算异构节点间相互作用的权重时增加边类型的嵌入向量,得到更合理的邻接矩阵;通过多头聚合多个子空间的邻居信息学习不同方式的特征交互;融合二阶以及三阶特征组合解决特征交互模块造成特征域的语义信息丢失问题,设计注意力模块抑制无用特征组合对模型学习的干扰;为进一步提升模型的性能,结合深度神经网络隐式捕捉高阶非线性的特征组合进行联合预测。实验结果表明,该方法优于其它主流点击率预测模型。

关键词：点击率预测邻接矩阵异构节点多空间聚合语义信息注意力模块深度神经网络