检索结果-南通市图书馆

Chemical Chaperones Increasing Expression Level of Soluble Single-chain Fv Antibody(scFv2F3)

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2007年第1期23卷 69-75页

作者： DU Xiu-bo SUN ye LIN Feng ZHENG Ke-yan WANG Ke-wei LIN Ting-ting LIU Jun-qiu SHEN jia-cong LUO Gui-min Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education Key Laboratory for Supramolecular Structure and Materials of the Ministry of Education Jilin University Changchun 130012 P. R. China

Glutathione peroxidase（GpX） is a sclenoenzyme that protects the biomembrane and other cellular components against oxidative damage. The selenium-containing single chain Fv fragment of monoelonal antibody 2173 （Se scFv2F3） is a kind of GpX mimic and it has a wide clinical applications because of its high activity and low antigenicity. Se-seFv2F3 is generated by the chemical modification of the single chain Fv fragment of monoelonal antibody 2F3 （ scFv2F3 ）, which can be expressed in E. coll. In this article, the effect of chemical chaperones, such as glycerol, glucose, and β-cyclodextrin added to the culture medium, on the expression of soluble scFv2F3 was investigated. The expression level was evaluated by the determination of soluble scFv2F3 contents in the whole cell lysates. The results suggest that both glycerol and β-cyclodextrin greatly increase the expression level of soluble scFv2F3, and β-cyclodextrin is found to be more effective compared with glycerol. Glucose has a slight effect on the expression level of soluble scFv2F3. This is the first example, wherein β-cyclodextrin has been used as a chemical chaperone during the cell culture to improve the expression level of recombinant proteins. In addition, chemical chaperones are found to decrease the toxic effect of IpTG on cells.

关键词： Glutathione peroxidase Soluble expression Glycerol Glucose β-Cyclodextrin

Association of T regulatory cells with natural course and response to treatment with interferon-α in patients with chronic hepatitis B infection

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2012年第8期125卷 1465-1468页

作者： XU Hong-tao XING Tong-jing LI Hao ye Jun Department of Infectious Diseases Taizhou People's HospitalTaizhouJiangsu 225300China

Background Regulatory T cell populations, particularly CD4＋CD25＋ T regulatory cells, have been implicated in the persistence of hepatitis B virus （HBV） infection. However, no clear relationship has been established between the frequency of CD4~CD25~ T regulatory cells in the peripheral blood and either the disease phases in the natural history of chronic HBV infection or in the response to interferon-a therapy. Methods In the present study, three different common markers of CD4＋CD25＋ T regulatory cells were used to determine the numbers of T regulatory cells in healthy controls and in patients with chronic HBV infection. Results No significant difference was found when samples were gated for CD25hi and CD25＋Foxp3＋ T cells. A significant correlation was found between the number of CD4＋ Treg cells that gated with CD25＋Foxp3＋ and CD25＋CD127low/- in healthy controls and in patients with chronic hepatitis B （CHB）（r=0.67, 0.59; p 〈0.01）. The percentages of Treg cells were （8.56±2.01）% in asymptomatic carriers （Asc）, （8.74±3.04）% in inactive HBsAg carriers, （10.7±2.93）% in CHB and （7.42±1.28）% in healthy controls （F=-11.1, p 〈0.001）. The percentage of Treg cells in patients with CHB was higher than in asymptomatic HBV patients, inactive HBsAg carriers, or healthy controls （p 〈0.01）. The proportion of CD4＋CD25＋CD127 low/T cells in patients who responded to interferon-（] was （11.9±3.3）%, （9.1±2.4）% and （9.0±2.9）% at baseline, week 12 and week 24 after treatment, respectively （Z=2.42, p〈0.05; Z=2.67, p〈0.01）. Conclusions These results suggest that the proportion of the CD4＋CD25＋ regulatory T cells might be affected by the application of different markers in process to detect T regulatory cells. The frequency of Treg cells was increased in patients with CHB, which might be associated with the disease activity of these patients and contribute to prevention of extensive liver damage. A decline in Treg cells at week 12 of treatment might be associated with a better response to t

关键词： CD4+CD25+ regulatory T cells Foxp3 chronic hepatitis B interferon-α

Malnutrition associated factors on children under 5 years old in Lhaviyani Atoll,Maldives

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2020年第4期34卷 301-308页

作者： Mariyam Asra Xiaofang Lin Ijaz Ul Haq Asfia Banu pasha Yingying Shao Rui Qin Manli Hu ye Ding Zhixu Wang Department of Maternal Child and Adolescent HealthSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of Nutrition and Food Hygiene School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Department of pediatrics the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China

In this report,we aimed to analyze the prevalence of undernutrition and associated factors among children under 5 years of age in Lhaviyani Atoll,Maldives.A total of 800 children(under 5 years old) and their mothers were selected for this *** was collected by using a pretested questionnaire and anthropometric measurements were taken from the hospital record ***-square tests and multivariate logistic regression were used to find the association between nutritional status and *** distribution of height for age and weight for age in surveyed children in Maldives was skewed to the left compared with the WHO *** prevalence of undernutrition based on underweight(10.75%),stunting(13.5%),and wasting(9.60%) was estimated to be 23.85% among *** age,gender and mother’s education were significantly associated with undernutrition(pprove child health in this region.

关键词： Maldives undernutrition stunting

Tamoxifen and clomiphene inhibit SARS-CoV-2 infection by suppressing viral entry

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第1期7卷 34-37页

作者： Shulong Zu Dan Luo Lili Li Qing ye Rui-Tinq Li Yanan Wang Meilinq Gao Heng Yang Yong-Qiang Deng Genhong Cheng Center for Systems Medicine Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Institute of Systems Medicine Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Suzhou Institute of Systems Medicine SuzhouJiangsuChina State Key Laboratory of pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyAcademy of Military Medical SciencesBeijingChina Suzhou Func Biotech Inc SuzhouJiangsuChina Department of Microbiology Immunology and Molecular GeneticsUniversity of CaliforniaLos AngelesCAUSA

Dear Editor,COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still a threat to millions of lives *** SARS-CoV-2 vaccines have been approved to reduce the severity and death associated with COVID-19,the number of SARS-CoV-2-infected cases still remains high,especially with the appearance of various mutant strains such as p.1.351 and p.1.617(also known as South Africa strain and India strain,respectively),which may reduce the efficacy of vaccine *** is an urgent need to develop effective antiviral agents to treat C0VID-19 patients,especially with those infected with SARS-CoV-2 variants of concern.

关键词： acute vaccine respiratory

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

北疆地区全新世气候波动与水热组合特点

中国沙漠 2000年第2期20卷 185-191页

作者：叶玮中国科学院新疆生态与地理研究所浙江师范大学地理系浙江金华321004

湖沼沉积、黄土沉积和冰积等地质记录的研究表明 ,北疆地区全新世气候受地理位置和大气环流影响 ,气候变化较为复杂。整个北疆地区早全新世气候暖干 ,中全新世至现代 ,气候特点因地因时而异。受西风控制地区 ,代表暖干环境的黄土沉积可... 详细信息

湖沼沉积、黄土沉积和冰积等地质记录的研究表明 ,北疆地区全新世气候受地理位置和大气环流影响 ,气候变化较为复杂。整个北疆地区早全新世气候暖干 ,中全新世至现代 ,气候特点因地因时而异。受西风控制地区 ,代表暖干环境的黄土沉积可一直延伸到晚全新世 ,而可能受夏季风影响的地区和偏北的阿尔泰山 ,环境的好转早于西风区。

关键词：北疆地区全新世气候特点水热组合特点古气候

慢性阻塞性肺疾病急性加重期患者焦虑抑郁现况及其影响因素

在线全文

学校读者我要写书评

暂无评论

中国呼吸与危重监护杂志 2024年第7期23卷 457-464页

作者：裴文婧李萍汪若文刘辉刘鹏程高磊马长秀郑凌张习伟孙晓赵大海叶静安徽医科大学第二附属医院呼吸与危重症医学科合肥综合性国家科学中心人工智能研究院(安徽省人工智能实验室)

目的了解慢性阻塞性肺疾病（简称慢阻肺）急性加重患者焦虑抑郁现况，并分析相关影响因素。方法对2022年8月—2023年10月入住安徽医科大学第二附属医院呼吸与危重症医学科的慢阻肺急性加重患者进行问卷调查。调查包括基本人口学信息... 详细信息

目的了解慢性阻塞性肺疾病（简称慢阻肺）急性加重患者焦虑抑郁现况，并分析相关影响因素。方法对2022年8月—2023年10月入住安徽医科大学第二附属医院呼吸与危重症医学科的慢阻肺急性加重患者进行问卷调查。调查包括基本人口学信息、焦虑和抑郁评分、慢阻肺患者生活质量及呼吸困难症状，同时收集电子病历系统中患者的临床资料，根据焦虑或抑郁评分将慢阻肺患者分为焦虑组和无焦虑组或抑郁组和无抑郁组，比较组间各项观察指标并分析影响因素。结果 164例慢阻肺急性加重患者中，合并焦虑的患者123例（75.0%），合并抑郁的患者125例（76.2%），同时合并焦虑抑郁有105例，占64.0%。受教育程度、居住地、月收入、吸烟指数、慢性阻塞性肺疾病评估测试（chronic obstructive pulmonary disease assessment test,CAT）评分与慢阻肺急性加重合并焦虑有关（均pphil percentage,NEU%）、淋巴细胞百分比（lymphocyte percentage,LYM%）、嗜碱性粒细胞百分比（basophile percentage,BASO%）、碱性磷酸酶、总二氧化碳浓度、中性粒细胞淋巴细胞比值（neutrophil to lymphocyte ratio,NLR）、血小板淋巴细胞比值（platelet to lymphocyte ratio,pLR）与慢阻肺急性加重合并抑郁有关（均ppLR值是慢阻肺急性加重合并抑郁的独立危险因素。结论慢阻肺急性加重患者中合并焦虑抑郁的患病率较高，影响因素有慢阻肺患者生活质量、呼吸困难症状、受教育程度、居住地、月收入、吸烟指数、酸碱平衡、NEU%、LYM%、BASO%、NLR和pLR等。

关键词：慢性阻塞性肺疾病急性加重焦虑抑郁慢性阻塞性肺疾病评估测试血小板淋巴细胞比值

Sequential ultrasound molecular imaging for noninvasive identification and assessment of non-alcoholic steatohepatitis in mouse models

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Liver Research 2023年第4期7卷 342-351页

作者： Tingting Sha Yujia You Xiaoyan Miao Huan Deng Wei Zhang Huolin ye ping Wang Rongqin Zheng Jie Ren Tinghui Yin Department of Medical Ultrasound Laboratory of Novel Optoacoustic(Ultrasonic)ImagingThe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdongChina Department of Medical Ultrasound Chengdu Women's and Children's Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduSichuanChina Department of Medical Ultrasonics The Third Affiliated Hospital of Southern Medical UniversityAcademy of OrthopedicsGuangzhouGuangdongChina

Background and objective:Noninvasive non-alcoholic steatohepatitis(NASH)assessment is a clinical challenge to the management of non-alcoholic fatty liver *** aim to develop diagnostic models based on sequential ultrasound molecular imaging(USMI)for the noninvasive identification of NASH in mouse ***:Animal experiments were approved by the Animal Ethics Committee of South China Agricultural ***-nine C57BL/6 mice were divided into normal control,non-alcoholic fatty liver,NASH,and hepatitis *** USMI was implemented using CD36-targeted microbubbles(MBs-CD36)and intercellular adhesion molecule-1(ICAM-1)-targeted microbubbles(MBs-ICAM-1)to visualize hepatic steatosis and *** targeting signal of USMI was quantified as the normalized intensity difference(NID)with the destruction-replenishment *** analysis was conducted between the NID-MBs-CD36 and pathological steatosis score and between the NID-MBsICAM-1 and pathological inflammation ***,diagnostic models combining NID-MBs-CD36 with NID-MBs-ICAM-1 were established for NASH ***:MBs-CD36 and MBs-ICAM-1 were successfully prepared and used for sequential USMI in all ***-MBs-CD36 values increased with the progression of steatosis,while NID-MBs-ICAM-1 values increased in parallel with the progression of inflammation.A strong positive correlation was identified between NID-MBs-CD36 and pathological steatosis grade(r_(s)=0.9078,ppathological inflammation grade(r_(s)=0.9071,pperformance in detecting NASH,with 95%sensitivity,97%specificity,95%positive predictive values,97%negative predictive values,and 96%***:Sequential USMI using MBs-CD36 and MBs-ICAM-1 allows noninvasive grading of hepatic steatosis and *** USMI-based diagnostic models hold great p

关键词： Non-alcoholic steatohepatitis(NASH) Ultrasound molecular imaging(USMI) CD36 Intercellular adhesion molecule-1(ICAM-1) Noninvasive diagnosis

透析中关节活动度运动对维持性血液透析患者疲乏症状和运动能力的影响研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国血液净化 2024年第7期23卷 552-556页

作者：李露王丽梅齐佳红叶立卉郑园华张翀旎南昌大学第一附属医院肾内科血液透析中心南昌330006 南昌大学护理学院南昌330006

目的探讨透析中关节活动度运动对维持性血液透析(maintenance hemodialysis,MHD)患者的影响。方法选取2023年4月─10月南昌大学第一附属医院血液透析中心的MHD患者为研究对象,由研究者随机投掷硬币将东湖院区和象湖院区随机分为试验组... 详细信息

目的探讨透析中关节活动度运动对维持性血液透析(maintenance hemodialysis,MHD)患者的影响。方法选取2023年4月─10月南昌大学第一附属医院血液透析中心的MHD患者为研究对象,由研究者随机投掷硬币将东湖院区和象湖院区随机分为试验组和对照组,对照组患者给予常规护理,试验组患者在常规护理的基础上实施透析中关节活动度运动。比较2组患者干预前后的疲乏量表-14(fatigue scale-14,FS-14)得分、6分钟步行试验(6 minutes walk test,6MWT)、30秒坐立试验(30-second sit-to-stand test,30s-STS)和简短生活质量量表得分(36-item short form health survey,SF-36)差异。结果透析中关节活动度运动干预后试验组患者的FS-14得分低于对照组(Z=-7.782,pppp=0.012)高于对照组。结论透析中关节活动度运动可有效缓解患者的疲乏症状,提高运动能力、生活质量。

关键词：维持性血液透析患者透析中运动关节活动度疲乏运动能力

高压氧早期干预对缺氧缺血性脑病新生儿行为神经评分及智能发育商的影响

在线全文

学校读者我要写书评

暂无评论

中国临床康复 2005年第23期9卷 194-195页

作者：叶红廉江市人民医院儿科广东省廉江市524400

目的:观察早期应用高压氧干预后缺氧缺血性脑病新生儿行为神经评分及对1年后智能发育商的变化。方法:选择2002-01/2004-03收治于廉江市人民医院儿科窒息合并缺氧缺血性脑病新生儿80例。随机分为2组,常规药物组40例和高压氧早期干预组40... 详细信息

目的:观察早期应用高压氧干预后缺氧缺血性脑病新生儿行为神经评分及对1年后智能发育商的变化。方法:选择2002-01/2004-03收治于廉江市人民医院儿科窒息合并缺氧缺血性脑病新生儿80例。随机分为2组,常规药物组40例和高压氧早期干预组40例。常规药物组严格按照儿科治疗手册中常规3项支持疗法和3项对症处理进行早期治疗,高压氧早期干预组同时早期给予高压氧l治疗。高压氧舱为上海701所研制的SHC-1800小型氧舱,给予一级吸氧,治疗压力为0.08Mpa,升压30min,减压30min,稳压40min,中间休息1次,合计100min,1次/d,10～14d为1个疗程,可治疗2～3个疗程。采用中国新生儿20项行为神经评分法,对所有患儿分别于治疗前、治疗1个疗程后各测定1次行为神经评分(满分为40分,pppppp<0.05)。结论:高压氧早期干预治疗能提高缺氧缺血性脑病新生儿的行为神经评分及1年后智能发育商。高压氧可提高血氧分压、加大氧弥散半径、改善组织有氧代谢及组织微循环,有利于脑组织的存活和受损神经细胞的修复,可明显改善患儿的预后。

关键词：氧吸入疗法/高压氧窒息新生儿智力测验