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Field released transgenic papaya effect on soil microbial communities and enzyme activities

Journal of Environmental Sciences 2006年第4期18卷 734-740页

作者： WEI Xiang-dong ZOU Hui-ling CHU Lee-min LIAO Bin ye chang-min LAN Chong-yu School of Life Sciences Sun Yat-sen University Guangzhou 510275 China Department of Biology The Chinese University ofHong Kong Shatin Hong Kong China

Soil properties, microbial communities and enzyme activities were studied in soil amended with replicase （RP）-transgenic or non-transgenic papaya under field conditions. Compared with non-transgenic papaya, significant differences （P〈0.05） were observed in total nitrogen in soils grown with transgenic papaya. There were also significant differences （P〈0.05） in the total number of colony forming units （CFUs） of bacteria, actinomycetes and fungi between soils amended with RP-transgenic plants and non-transgenic plants. Compared with non-transgenic papaya, the total CFUs of bacteria, actinomycetes and fungi in soil with transgenic papaya increased by 0.43-1.1, 0.21-0.80 and 0.46-0.73 times respectively. Significantly higher （P〈0.05） CFUs of bacteria, actinomycetes and fungi resistant to kanamycin （Km） were obtained in soils with RP-transgenic papaya than those with non-transgenic papaya in all concentrations of Km. Higher resistance quotients for Km＇（kanamycin resistant） bacteria, actinomycetes and fungi were found in soil planted with RP-transgenic papaya, and the resistance quotients for Km＇ bacteria, actinomycetes and fungi in soils with transgenic papaya increased 1.6-4.46, 0.63-2.5 and 0.75-2.30 times. RP-transgenic papaya and non-transgenic papaya produced significantly different enzyme activities in arylsulfatase （5.4-5.9x）, polyphenol oxidase （0.7-1.4x）, invertase （0.5-0.79x）, cellulase （0.23-0.35x） and phosphodiesterase （0.16-0.2x）. The former three soil enzymes appeared to be more sensitive to the transgenic papaya than the others, and could be useful parameters in assessing the effects of transgenic papaya. Transgenic papaya could alter soil chemical properties, enzyme activities and microbial communities.

关键词： transgenic papaya soil microbes enzyme activity kanamycin resistance resistance quotient

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阴道彩超检测子宫内膜厚度及其血流鉴别宫内外早孕的临床价值

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智慧健康 2021年第8期7卷 1-3页

作者：张婷叶昌敏苟元林巴中市中医院超声科四川巴中636000 巴中市恩阳区人民医院超声科四川巴中636000

目的探讨阴道彩超检测子宫内膜厚度及其血流鉴别宫内外早孕的临床价值。方法以我院于2018年8月至2020年8月收治的80例早孕期孕妇作为本次研究的观察主体,80例早孕孕妇均进行腹部彩超检查和阴道彩超检查,将其检查结果与最终确诊结果相比... 详细信息

目的探讨阴道彩超检测子宫内膜厚度及其血流鉴别宫内外早孕的临床价值。方法以我院于2018年8月至2020年8月收治的80例早孕期孕妇作为本次研究的观察主体,80例早孕孕妇均进行腹部彩超检查和阴道彩超检查,将其检查结果与最终确诊结果相比较,并观察分析早孕期孕妇的子宫内膜厚度以及血流情况。结果经腹部彩超检查结果显示,确诊为宫内早孕的55例(68.75%),宫外早孕25例(31.25%),宫外早孕漏检14例(17.50%),经阴道彩超检查结果显示,确诊为宫内早孕的42例(52.50%),宫外早孕38例(47.50%),宫外早孕漏检1例(1.25%),阴道彩超检查宫外早孕的检出率明显高于腹部彩超检查,阴道彩超检查宫外早孕漏检率明显低于腹部彩超检查,比较差异显著(P1.5cm占比较大,子宫内膜血流血流阻力指数0.5占比较大,比较差异显著(P<0.05)。结论在宫内外早孕临床鉴别中,通过阴道彩超对患者的子宫内膜厚度及其血流情况进行检测,并根据声像特征,可提高宫外孕检出率。

关键词：宫内早孕宫外早孕阴道彩超子宫内膜厚度

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ANA和ENA检测在自身免疫性疾病中的应用评价

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现代免疫学 2011年第1期31卷 56-59页

作者：陆慧琦李畅叶伟民杨洁何铭珺韩焕兴第二军医大学长征医院实验诊断科上海200003

探讨抗核抗体和抗可溶性核抗原抗体的不同检测方法对自身免疫性疾病诊断的指导意义。回顾性分析2007年1月至2009年10月间在长征医院就诊的患者血清自身抗体的检测结果,549位患者,其中自身免疫病患者224例,非自身免疫病325例,所有患者血... 详细信息

探讨抗核抗体和抗可溶性核抗原抗体的不同检测方法对自身免疫性疾病诊断的指导意义。回顾性分析2007年1月至2009年10月间在长征医院就诊的患者血清自身抗体的检测结果,549位患者,其中自身免疫病患者224例,非自身免疫病325例,所有患者血清同时检测ANA和ENA。以Hep-2细胞/肝组织为基质的间接免疫荧光法检测ANA,免疫印迹法检测ENA。两种检测方法产生4种检出模式:ANA+/ENA+、ANA-/ENA-、ANA+/ENA-和ANA-/ENA+。前两种模式共占检测的62.84%。ANA和ENA在自身免疫病患者中的阳性率(63.4%和58.5%)显著高于非自免病患者(16.9%和25.2%),ANA和ENA在自身免疫病组和非自身免疫病组间阳性率比较,差异有统计学意义(P0.05)。间接免疫荧光法相对费时,而且需要操作者具备一定的经验,作为初筛实验,ANA的检测较ENA有更高的灵敏度,两者联合检测则将有利于提高检测的灵敏度和可靠性。

关键词：抗核抗体抗可溶性核抗原间接免疫荧光法斑点法

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Regularity of Solutions to Elliptic Equations with VMO Coefficients

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Acta Mathematica Sinica,English Series 2004年第6期20卷 1103-1118页

作者： ye min CHEN LMAM School of Mathematical SciencePeking University

The aim of this paper is to study the regularity of solutions to the Dirichlet problems for general second-order elliptic equations in Lebesgue and Morrey *** consider both non- divergence and divergence forms and the... 详细信息

关键词： Regularity Elliptic equations VMO

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Representations of Frobenius-type Triangular Matrix Algebras

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Acta Mathematica Sinica,English Series 2017年第3期33卷 341-361页

作者： Fang LI chang ye Department of Mathematics Zhejiang University Hangzhou 310027 P. R. China

The aim of this paper is mainly to build a new representation-theoretic realization of finite root systems through the so-called Frobenius-type triangular matrix algebras by the method of reflection functors over any field. Finally, we give an analog of APR-tilting module for this class of algebras. The major conclusions contains the known results as special cases, e.g., that for path algebras over an algebraically closed field and for path algebras with relations from symmetrizable cartan matrices. Meanwhile, it means the corresponding results for some other important classes of algebras, that is, the path algebras of quivers over Frobenius algebras and the generalized path algebras endowed by Frobenius algebras at vertices.

关键词： Frobenius-type triangular matrix algebras reflection functor locally free module rootsystem APR-tilting module

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Luminosity of a radio pulsar and its new emission death line

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Research in Astronomy and Astrophysics 2020年第11期20卷 325-332页

作者： Qing-Dong Wu Qi-Jun Zhi Cheng-min Zhang De-Hua Wang chang-Qing ye School of Physics and Electronic Sciences Guizhou Normal UniversityGuiyang 550001China National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Guizhou Provincial Key Laboratory of Radio Astronomy and Data Processing Guiyang 550001China University of Chinese Academy of Sciences Beijing 100049China

We investigated the pulsar radio luminosity(L),emission efficiency(ratio of radio luminosity to its spin-down powerE),and death line in the magnetic field(B)versus spin period(P)*** found that the dependence of pulsar radio luminosity on its spin-down power(L-E)is very weak,shown as L^E0.06,which deduces an equivalent inverse correlation between emission efficiency and spin-down power asξ~***,we examined the distributions of radio luminosity of millisecond and normal pulsars and found that for the similar spin-down powers,the radio luminosity of millisecond pulsars is about one order of magnitude lower than that of the normal *** analysis of pulsar radio flux suggests that these correlations are not due to a selective effect but are intrinsic to the pulsar radio emission *** radio radiations may be dominated by the different radiation *** cutoff phenomenon of currently observed radio pulsars in B-P diagram is usually referred as the"pulsar death line",which corresponds toE≈1030 erg s-1 and is obtained by the cut-off voltage of electron acceleration gap in the polar cap model of pulsar proposed by Ruderman and ***,this death line can be inferred by the actual observed pulsar flux S≥1 mJy and 1 kpc distance,together with the maximum radio emission efficiency of 1%.However,the observation data show that the 37 pulsars pass over the death line,including the recently observed two pulsars with long periods of 23.5 s and 12.1 s,which violate the prediction of the polar cap *** present,the actual observed pulsar flux can reach0.01 mJy by FAST *** will arise the observational limit of spin-down power of pulsars as low as E˙≈1028 erg *** means that the new death line is downward shifted two orders of magnitude,which might be favorably referred as the"observational limit–line".Accordingly,the pulsar theoretical model for the cut-off voltage of gap should be heavily modified.

关键词： stars neutron pulsars general stars fundamental parameters

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Expression of lens-related microRNAs in transparent infant lenses and congenital cataract

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International Journal of Ophthalmology(English edition) 2017年第3期10卷 361-365页

作者： chang-Rui Wu min ye Li Qin Yue Yin Cheng Pei Department of Ophthalmology the First Affiliated Hospital of Xi’an Jiaotong University Ningxia Eye Hospital People Hospital of Ningxia Hui Autonomous Region(First Affiliated Hospital of Northwest University for Nationalities) Basic Research Center Affiliated Shaanxi Provincial Tumor HospitalCollege of MedicineXi’an Jiaotong University

AIM：To identify the expression of lens-related micro RNAs（miRNAs）in the central epithelium of transparent infant lenses and congenital cataract. METHODS：Lens-related mi RNAs were retrieved from Pub Med database. The expression levels of these mi RNAs in transparent infant lenses and congenital cataract were determined by stem-loop reverse transcription-polymerase chain reaction（RT-PCR）. mi Randa algorithm was used to predict the target genes of these differentially expressed mi RNAs. The target m RNA was ***：Six lens-related mi RNAs were retrieved from screening Pub Med database. The most abundant mi RNA in transparent infant lenses according to stem-loop RT-PCR was mi R-184. miR-182 was up-regulated in congenital cataract. Contrarily,miR-204 and miR-124 was *** R-204 exhibited a more significant decrease in expression than mi R-124. In addition,Meis2 was predicted to be the target of mi R-204 using mi Randa algorithm. mi R-204mimic/antagomir transfection experiments suggested the negative correlation between the expression of mi R-204 and ***：The expression levels of miR-182,miR-204 and mi R-124 differ between the central epithelium of transparent infant lens and congenital cataract,suggesting their involvement in the pathogenesis of congenital cataract. miR-204 may act via silencing Meis2 to regulate lens development and congenital cataract formation.

关键词： lens-related miRNAs congenital cataract miR-204 Meis2

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Cluster Structures in 2-Calabi-Yau Triangulated Categories of Dynkin Type with Maximal Rigid Objects

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Acta Mathematica Sinica,English Series 2017年第12期33卷 1693-1704页

作者： Hui min chang Department of Mathematical Sciences Tsinghua University Beijing 100084 P. R. China Department of Applied Mathematics School of Education The Open University of China Beijing 100039 Po R. China

In this paper, we consider two kinds of 2-Calabi-Yau triangulated categories with finitely many indecomposable objects up to isomorphisms, called An,t =D^b（KA（2t＋1）（n＋1）-3）/τ^t（n＋1）-1[1], where n,t ≥ 1, and Dn,t = Db（KD2t（n＋1））/τ^（n＋1）φ^n, where n,t ≥ 1, and φ is induced by an automorphism of D2t（n＋1） of order 2. Except the categories An,1, they all contain non-zero maximal rigid objects which are not cluster tilting. An,1 contain cluster tilting objects. We define the cluster complex of An,t （resp. Dn,t） by using the geometric description of cluster categories of type A （resp. type D）. We show that there is an isomorphism from the cluster complex of An,t （resp. Dn,t） to the cluster complex of root system of type Bn. In particular, the maximal rigid objects are isomorphic to clusters. This yields a result proved recently by Buan-Palu-Reiten： Let RAn,t, resp. RDn,t, be the full subcategory of An,t, resp. Dn,t, generated by the rigid objects. Then RAn,t≈RAn,1 and TDn,t≈TAn,1 as additive categories, for all t 〉 1.

关键词： 2-Calabi-Yau triangulated category cluster structure cluster complex

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Hydrogen storage properties of preferentially orientated Mg-Ni multilayer film prepared by magnetron sputtering

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Rare Metals 2006年第z1期25卷 295-299页

作者： ye Suyun OUYANG Liuzhang ZHU min College of Mechanical Engineering South China University of Technology Guangzhou 510640 China College of Mechanical Engineering South China University of Technology Guangzhou 510640 China College of Mechanical Engineering South China University of Technology Guangzhou 510640 China

Mg-Ni multi-layer thin film was deposited on (001) Si wafer by magnetron sputtering with dual-target. X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis reveal that the microstructure of the Mg-Ni multilayer thin film is composed of fine-crystalline Ni layer and crystalline [001] Mg layer. Hydrogenation process of the films were carried out by using the automatic gas reaction controller. The films undergone hydrogenation for different time were analyzed by XRD. The results show that hydrogenation properties of Mg with different preferential orientations are different. (002) diffraction peak of Mg disappears in compensating the appearing of the peaks of Mg2NiH4 and MgH2 in hydrogenation at 533 K, while the (101) peak still remains. The result reveals that the Mg film with (001) preferential orientation absorbs hydrogen at certain temperature easier than that of the Mg film with (101) orientation. This phenomenon can be explained in the view point of the energy change for the nucleation and growth of hydride in different crystal plane.

关键词： hydrogen storage Mg-Ni film multi-layers magnetron sputtering

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A multi-point collaborative DDoS defense mechanism for IIoT environment

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Digital Communications and Networks 2023年第2期9卷 590-601页

作者： Hongcheng Huang Peixin ye min Hu Jun Wu School of Communication and Information Engineering Chongqing University of Posts and TelecommunicationsChongqing400065China Chongqing Engineering Research Center of Communication Software Chongqing400065China

Nowadays,a large number of intelligent devices involved in the Industrial Internet of Things(IIoT)environment are posing unprecedented cybersecurity *** to the limited budget for security protection,the IIoT devices are vulnerable and easily compromised to launch Distributed Denial-of-Service(DDoS)attacks,resulting in disastrous ***,considering the particularity of the IIoT environment,most of the defense solutions in traditional networks cannot be directly applied to IIoT with acceptable security ***,in this work,we propose a multi-point collaborative defense mechanism against DDoS attacks for ***,for the single point DDoS defense,we design an edge-centric mechanism termed EdgeDefense for the detection,identification,classification,and mitigation of DDoS attacks and the generation of defense *** the practical multi-point scenario,we propose a collaborative defense model against DDoS attacks to securely share the defense information across the network through the ***,a fast defense information sharing mechanism is designed to reduce the delay of defense information sharing and provide a responsive cybersecurity *** simulation results indicate that the identification and classification performance of the two machine learning models designed for EdgeDefense are better than those of the state-of-the-art baseline models,and therefore EdgeDefense can defend against DDoS attacks *** results also verify that the proposed fast sharing mechanism can reduce the propagation delay of the defense information blocks effectively,thereby improving the responsiveness of the multi-point collaborative DDoS defense.

关键词： Industrial internet of things(IIoT) DDoS Deep learning Blockchain Edge computing

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