Expression of lens-related microRNAs in transparent infant lenses and congenital cataract

作     者：Chang-Rui Wu Min Ye Li Qin Yue Yin Cheng Pei 

作者机构：Department of Ophthalmologythe First Affiliated Hospital of Xi’an Jiaotong University Ningxia Eye HospitalPeople Hospital of Ningxia Hui Autonomous Region(First Affiliated Hospital of Northwest University for Nationalities) Basic Research CenterAffiliated Shaanxi Provincial Tumor HospitalCollege of MedicineXi’an Jiaotong University 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2017年第10卷第3期

页      面：361-365页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by the Natural Science Foundation of China(No.81470614) the Fundamental Research Funds for the Central Universities sponsored by Xi’an Jiaotong University(No.xjj2013067) Youth Foundation of the First Affiliated Hospital,Medical College,Xi’an Jiaotong University(No.2014YK7) Scientific Research Funds for the Health and Family Planning of Shaanxi Province(No.2016D068) 

主　　题：lens related miRNAs congenital cataract miR 204 Meis2 

摘      要：AIM：To identify the expression of lens-related micro RNAs（miRNAs）in the central epithelium of transparent infant lenses and congenital cataract. METHODS：Lens-related mi RNAs were retrieved from Pub Med database. The expression levels of these mi RNAs in transparent infant lenses and congenital cataract were determined by stem-loop reverse transcription-polymerase chain reaction（RT-PCR）. mi Randa algorithm was used to predict the target genes of these differentially expressed mi RNAs. The target m RNA was ***：Six lens-related mi RNAs were retrieved from screening Pub Med database. The most abundant mi RNA in transparent infant lenses according to stem-loop RT-PCR was mi R-184. miR-182 was up-regulated in congenital cataract. Contrarily,miR-204 and miR-124 was *** R-204 exhibited a more significant decrease in expression than mi R-124. In addition,Meis2 was predicted to be the target of mi R-204 using mi Randa algorithm. mi R-204mimic/antagomir transfection experiments suggested the negative correlation between the expression of mi R-204 and ***：The expression levels of miR-182,miR-204 and mi R-124 differ between the central epithelium of transparent infant lens and congenital cataract,suggesting their involvement in the pathogenesis of congenital cataract. miR-204 may act via silencing Meis2 to regulate lens development and congenital cataract formation.