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Synthesis and characterization of molecular sieve Ni-ZSM-5 and its single crystal

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Chinese Journal of Chemistry 1991年第2期9卷 109-115页

作者： yao,shang-qing qiu,shi-lun pang,wen-qin SUN,Tie JIANG,Da-Zhen Department of Chemistry,Jilin University,Changchun 130023 Department of Chemistry Jilin University Changchun 130023

Using tetrapropylammonium bromide(TPABr)as a template,molecular sieve Ni-ZSM-5 and its single crystals were synthesized in a nonalkaline system in the presence of fluoride *** means of XRD,IR,EPMA,MAS NMR,EPR,DTA and H_2-TPR techniques,it is proved that the hetero-atom,nickel,is really located in the zeolite *** finally,Ni-ZSM-5 was found to have high catalytic cracking reactivity.

关键词： SM Synthesis and characterization of molecular sieve Ni-ZSM-5 and its single crystal Ni

瓯江口海域鱼类早期资源群落结构及优势种相关性分析

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水生生物学报 2024年第6期48卷 898-909,I0004-I0006页

作者：李尚清叶深王雨航唐未秦松范青松邱文致艾龙威郑春芳刘伟成温州大学生命与环境科学学院温州325035 浙江省海洋水产养殖研究所温州325005 浙江省近岸水域生物资源开发与保护重点实验室温州325005

为掌握瓯江口海域鱼类早期资源现状及其与主要环境因子之间的关系,基于2021年春季(5月)、夏季(8月)和秋季(11月)对瓯江口海域内25个站位的表层水平拖网数据和温度、盐度及pH等基础水文调查数据,对该海域鱼卵、仔稚鱼的物种优势度、生物... 详细信息

为掌握瓯江口海域鱼类早期资源现状及其与主要环境因子之间的关系,基于2021年春季(5月)、夏季(8月)和秋季(11月)对瓯江口海域内25个站位的表层水平拖网数据和温度、盐度及pH等基础水文调查数据,对该海域鱼卵、仔稚鱼的物种优势度、生物多样性和群落结构特征等进行分析,同时探讨优势种侧带小公鱼属鱼类(Stolephorus spp.)资源分布与环境因子之间的关系。结果表明:3个航次调查共采集鱼卵8427粒,仔稚鱼40365尾,共鉴定49种,隶属于12目23科39属,以鲈形目(Perciformes)为主要类群,在发育阶段上以前弯曲期仔鱼居多,按照生态类型划分主要以海洋性与河口性鱼类为主。调查海域内不同季节采获鱼卵、仔稚鱼的种类、数量和密度有所差异,平均密度在0.07—4.72粒(尾)/m^(3),春季最高分别为34种、38083粒(尾)和4.72粒(尾)/m^(3)。在空间分布上,鱼卵沿着瓯江口由内向外呈递增趋势,而仔稚鱼则呈递减趋势。同时各季节鱼卵、仔稚鱼生物多样性的差异性较为显著,春季的多样性指数(H’)和均匀度指数(J’)均为最高,丰富度指数(D)最高值出现在夏季。调查海域内各季节鱼卵、仔稚鱼的优势种组成差异较大,除共有优势种侧带小公鱼属鱼类外,春季、夏季和秋季航次的主要优势种分别为虾虎鱼科鱼类(Gobiidae spp.)、凤鲚(Coilia mystus Linnaeus)和舌鳎属鱼类(Cynoglossus sp.1)。利用相对重要性指数IRI计算物种优势度,结果显示侧带小公鱼属鱼类的优势度在所有航次中均为最高,通过分析其与环境因子的关系,发现与盐度的相关性最高(P<0.01),与温度相关性最低(P<0.05)。经与历史资料对比,推测春季北龙岛附近海域是鱼类重要的产卵场,高温高盐弱碱的水文环境更有利于侧带小公鱼属鱼类的生长和繁育,这为瓯江口海域鱼类资源养护管理和渔业生态环境的保护提供了参考依据。

关键词：鱼卵仔稚鱼优势种群落结构环境因子瓯江口

Efficacy of Moxifloxacin against Mycobacterium abscessus in Zebrafish Model in vivo

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Biomedical and Environmental Sciences 2020年第5期33卷 350-358页

作者： NIE wen Juan XIE Zhong yao GAO Shan TENG tian Lu ZHOU wen Qiang shang Yuan Yuan JING Wei shi wen Hui WANG qing Feng HUANG Xue Rui CAI Bao Yun WANG Jun WANG Jing GUO Ru GE Qi Ping NIE Li Hui HAN Xi qin DU Ya Dong CHU Nai Hui Tuberculosis Department Beijing Chest Hospital affiliated to Capital Medical UniversityBeijing 101149China Beijing Chest Hospital affiliated to Capital Medical University Beijing 101149China

Objective Moxifloxacin(MFX)shows good in vitro activity against Mycobacterium abscessus and can be a possible antibiotic therapy to treat *** infection;however,other studies have shown a lower or no *** aimed to evaluate MFX activity against *** using zebrafish(ZF)model in *** A formulation of *** labeled with CM-Dil was micro-injected into *** curves were determined by recording dead ZF every *** were lysed,and colony-forming units(CFUs)were *** dissemination and fluorescence intensity in ZF were *** rates of MFX and azithromycin(AZM,positive control)were determined and *** Significantly increased survival rate was observed with different AZM ***,increasing MFX concentration did not result in a significant decrease in ZF survival *** significant differences in bacterial burdens by CFU loads were observed between AZM and MFX groups at various *** fluorescence intensity in ZF was significantly correlated with AZM ***,with increasing MFX concentration,fluorescence intensity decreased slightly when observed under fluorescence *** rates at various concentrations were comparable between the MFX and AZM groups,with no significant *** MFX showed limited efficacy against *** in vivo using ZF *** activity in vivo needs to be confirmed.

关键词： Mycobacterium abscessus Moxifloxacin Azithromycin Zebrafish

慢性牙周炎伴咬合创伤患者龈沟液Sclerostin、SLIT3与牙周临床指标和RANKL-OPG系统的相关性研究

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现代生物医学进展 2024年第1期24卷 83-86,105页

作者：秦高伟邱丽娜姜智雯张思瑶李慧吉林大学中日联谊医院口腔科吉林长春130033 郑州人民医院口腔科河南郑州450053 大连市口腔医院口腔综合科辽宁大连116021

目的:探讨慢性牙周炎伴咬合创伤患者龈沟液骨硬化蛋白(Sclerostin)、神经轴突导向因子3(SLIT3)与牙周临床指标和核因子-κb受体活化因子配体(RANKL)-骨保护蛋白(OPG)系统的相关性。方法:选择2021年1月至2023年1月我院收治的123例慢性牙... 详细信息

目的:探讨慢性牙周炎伴咬合创伤患者龈沟液骨硬化蛋白(Sclerostin)、神经轴突导向因子3(SLIT3)与牙周临床指标和核因子-κb受体活化因子配体(RANKL)-骨保护蛋白(OPG)系统的相关性。方法:选择2021年1月至2023年1月我院收治的123例慢性牙周炎伴咬合创伤患者(创伤组)和101例不伴咬合创伤的慢性牙周炎患者(对照组)。治疗前,检测龈沟液中Sclerostin、SLIT3、RANKL、OPG水平,计算RANKL/OPG比值,评估临床牙周指标出血指数(SBI)、菌斑指数(PLI)、探诊深度(PD)、附着丧失(AL)。分析Sclerostin、SLIT3与牙周临床指标、RANKL、OPG、RANKL/OPG之间的相关性。结果:创伤组治疗前龈沟液中Sclerostin、SLIT3、RANKL水平,RANKL/OPG,PLI、SBI、AL、PD高于对照组(P<0.05),OPG低于对照组(P<0.05)。创伤组治疗前龈沟液中Sclerostin、SLIT3与PLI、SBI、AL、PD、RANKL、RANKL/OPG呈正相关(P<0.05),与OPG呈负相关(P<0.05)。结论:慢性牙周炎伴咬合创伤患者龈沟液中Sclerostin、SLIT3水平显著增高,且与牙周临床指标异常、RANKL/OPG增加有关。

关键词：慢性牙周炎咬合创伤龈沟液 Sclerostin SLIT3 牙周临床指标 RANKL-OPG系统

Downregulation of microRNA-382 is associated with poor outcome of esophageal squamous cell carcinoma

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世界胃肠病学杂志：英文版 2015年第22期21卷 6884-6891页

作者： Bo Qi Jian-Guo Lu wen-Jian yao Ting-Min Chang Xiu-Guang qin Ying-Hua Ji tian-Yun Wang shang-Guo Liu Han-Chen Li Yu-Zhen Liu Bao-Sheng Zhao Department of Thoracic Surgery The First Affiliated Hospital of Xinxiang Medical University Department of Gastroenterology The First Affiliated Hospital of Xinxiang Medical University Department of Oncology The First Affiliated Hospital of Xinxiang Medical University Department of Molecular Biology Xinxiang Medical University

AIM: To study the potential prognostic role of microRNA-382(miR-382) in esophageal squamous cell carcinoma(ESCC).METHODS: Forty six patients were divided into 2groups according to postoperative survival time:the poor outcome group(28 patients), who showed early metastasis but no recurrence, and died within 1year after surgery, 12 patients of the group received postoperative chemotherapy treatment that was given after early metastasis happening; the good outcome group(18 patients), who had no clinical metastasis and recurrence, and survived 5 years or more after surgery, all patients did not receive any postoperative treatment. Total RNA was extracted from the patients' formalin-fixed and paraffin-embedded esophageal cancer tissues. miR-382 level was evaluated using highthroughput real-time quantitative polymerase chain reaction analysis. The correlation between miR-382 level and clinicopathologic features was analyzed through COX regression model, and Kaplan-Meier analysis was used to analyze the relationship betweenmiR-382 level and patient survival ***: miR-382 was differentially expressed in the two groups. Overall the average miR-382 level in the ESCC patients with good outcome was 9.8 ± 3.8,while miR-382 level in the ESCC patients with poor outcome was 3.0 ± 0.8. The differences of miR-382 levels between two groups were significant(P < 0.05).Kaplan-Meier analysis results showed that miR-382 expression level generally had a significant reversecorrelation with ESCC patient survival time(P <0.001), in which the patients with higher expressions of miR-382 had a longer survival time either among individuals with the same tumor stage or among the overall ***: miR-382 levels are reverse-correlated with ESCC poor outcomes, suggesting that miR-382 could be a potential predictive biomarker for both prognosis and treatment of ESCC.

关键词： Esophageal squamous cell carcinoma miR-382 Metastasis Outcome Prognosis

Taxonomy and phylogeny of hyaline‑spored coelomycetes

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Fungal Diversity 2020年第1期100卷 279-801页

作者： wen‑Jing Li Eric H.C.McKenzie Jian‑Kui(Jack)Liu D.Jayarama Bhat Dong‑qin Dai Erio Camporesi qing tian Sajeewa S.N.Maharachchikumbura Zong‑Long Luo qiu‑Ju shang Jin‑Feng Zhang Narumon Tangthirasunun Samantha C.Karunarathna Jian‑Chu Xu Kevin D.Hyde Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China World Agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Mushroom Research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Mai 50150Thailand Landcare Research Manaaki Whenua Private Bag 92170AucklandNew Zealand School of Life Science and Technology University of Electronic Science and Technology of ChinaChengdu 611731People’s Republic of China No.128/1‑J Azad Cooperative Housing SocietyCurcaP.O.Goa VelhaGoa 403108India Center for Yunnan Plateau Biological Resources Protection and Utilization College of Biological Resource and Food EngineeringQujing Normal UniversityQujingYunnan 655011People’s Republic of China A.M.B.Gruppo Micologico Forlivese“Antonio Cicognani”Via Roma 18ForlìItaly A.M.B Circolo Micologico“Giovanni Carini” C.P.314BresciaItaly Societàper gli Studi Naturalistici della Romagna C.P.143BagnacavalloRAItaly College of Agriculture and Biological Sciences Dali UniversityDali 671003YunnanPeople’s Republic of China Department of Biology Faculty of ScienceKing Mongkut’s Institute of TechnologyLadkrabangChalongkrung Rd.Ladkrabang DistrictBangkok 10520Thailand

Coelomycete is a general term used for asexual fungi which produce conidia in fruiting bodies:pycnidial,acervular,cupulate,pycnothyria or stromatic *** group contains numerous plant pathogenic,saprobic and endophytic species associated with a wide range of ***,morphological characters and host associations have been used as criteria to identify and classify coelomycetes,and this has resulted in a poor understanding of their generic and species *** based taxonomic studies have provided a better outlook of the phylogenetic and evolutionary trends in ***,the present outcomes represent only a preliminary step towards the understanding of *** genera have not been revisited since they were first *** present study revises the classification of the hyaline-spored coelomycetes and provides a modern taxonomic framework based on both morphology and *** total,248 genera were investigated,of which less than 100 are known to have sequence ***-locus sequence data analyses of 28S nrDNA,18S nrDNA,ITS,RNA polymerase II second largest subunit(rpb2),and part of the translation elongation factor 1-alpha gene(tef1)andβ-tubulin(tub2)gene regions were *** a result,three new genera and 23 new species are *** addition,three new links between sexual and asexual genera are *** are 138 genera that lack sequence data,and these are treated as Ascomycota,genera incertae *** drawings and descriptions are provided based on the examination of types and fresh collections and on the literature.

关键词： 26 new taxa Ascomycota Asexual morphs Basidiomycota

Preliminary results of environmental monitoring of the natural gas hydrate production test in the South China Sea

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China Geology 2018年第2期1卷 202-209页

作者： Jian-liang Ye Xu-wen qin Hai-jun qiu Qian-yong Liang Yi-fei Dong Jian-gong Wei Hai-long Lu Jing-an Lu yao-hong shi Chao Zhong Zhen Xia Guangzhou Marine Geological Survey China Geological SurveyGuangzhou 510075China Gas Hydrate Engineering Technology Center China Geologtcal SurveyGuangzhou 510075China College of Engineering Peking UniversityBeijing 100871China

Natural gas hydrate (NGH)is considered as one of the new clean energy sources of the 21st century with the highest *** environmental issues of NGH production have attracted the close attention of scientists in various *** May 10 to July 9,2017,the first offshore NGH production test in the South China Sea (SCS)was conducted by the China Geological *** addition,environmental security has also been effectively guaranteed via a comprehensive environmental monitoring system built during the NGH production *** monitoring system considered sea-surface atmosphere methane and carbon dioxide concentrations,dissolved methane in the sea water column,and the seafloor physical oceanography and marine chemistry *** whole process was monitored via multiple means, in multiple layers,in all domains,and in real *** the production test,an environmental investigation was promptly conducted to evaluate the environmental impact of the NGH production test. The monitoring results showed that the dissolved methane concentration in seawater and the near-seabed environment characteristics after the test were consistent with the background values,indicating that the NGH production test did not cause environmental problems such as methane leakage.

关键词： South China Sea Natural gas hydrate Production test Methane leakage Environmental momtoring

Factors associated with a SARS-CoV-2 recurrence after hospital discharge among patients with COVID-19:systematic review and meta-analysis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第12期21卷 940-947页

作者： Meng-qi yao qiu-xian ZHENG Jia XU Jing-wen DENG tian-tian GE Hai-bo ZHOU Feng-tian WU Xin-yu GU qin YANG Yan-li REN Gang WANG Zhi CHEN Zhejiang University School of Public Health Hangzhou 310058China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infections Diseasesthe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China

Background:The proportion of recurrences after discharge among patients with coronavirus disease 2019(COVID-19)was reported to be between 9.1%and 31.0%.Little is known about this issue,however,so we performed a meta-analysis to summarize the demographical,clinical,and laboratorial characteristics of non-recurrence and recurrence ***:Comprehensive searches were conducted using eight electronic *** re-garding the demographic,clinical,and laboratorial characteristics of both recurrence and non-recurrence groups were extracted,and quantitative and qualitative analyses were ***:Ten studies involving 2071 COVID-19 cases were included in this *** proportion of recurrence cases involving patients with COVID-19 was 17.65%(between 12.38%and 25.16%)while older patients were more likely to experience recurrence(weighted mean difference(WMD)=1.67,range between 0.08 and 3.26).The time from discharge to recurrence was 13.38 d(between 12.08 and 14.69 d).Patients were categorized as having moderate severity(odds ratio(OR)=2.69,range between 1.30 and 5.58),while those with clinical symptoms including cough(OR=5.52,range between 3.18 and 9.60),sputum production(OR=5.10,range between 2.60 and 9.97),headache(OR=3.57,range between 1.36 and 9.35),and diz-ziness(OR=3.17,range between 1.12 and 8.96)were more likely to be associated with *** pre-senting with bilateral pulmonary infiltration and decreased leucocyte,platelet,and CD4+T counts were at risk of COVID-19 recurrence(OR=1.71,range between 1.07 and 2.75;WMD=?1.06,range between?1.55 and?0.57,WMD=?40.39,range between?80.20 and?0.48,and WMD=?55.26,range between?105.92 and?4.60,respectively).Conclusions:The main factors associated with the recurrence of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)after hospital discharge were older age,moderate severity,bilateral pulmonary infiltration,laboratory findings including decreased leucocytes,platelets,and CD4+T counts,a

关键词： Recurrence case Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) Risk factor Meta-analysis

Plasma metabolomic and lipidomic alterations associated with COVID-19

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National Science Review 2020年第7期7卷 1157-1168页

作者： Di Wu Ting Shu Xiaobo Yang Jian-Xin Song Mingliang Zhang Chengye yao wen Liu Muhan Huang Yuan Yu qingyu Yang Tingju Zhu Jiqian Xu Jingfang Mu Yaxin Wang Hong Wang Tang Tang Yujie Ren Yongran Wu Shu-Hai Lin Yang qiu Ding-Yu Zhang You shang Xi Zhou Joint Laboratory of Infectious Diseases and Health Wuhan Institute of Virology & Wuhan Jinyintan Hospital Wuhan Institute of Virology Center for Biosafety Mega-ScienceChinese Academy of Sciences (CAS) State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-Science CAS Center for Translational Medicine Jinyintan Hospital Joint Laboratory of Infectious Diseases and Health Wuhan Institute of Virology & Wuhan Jinyintan Hospital Wuhan Jinyintan Hospital Department of Critical Care Medicine Union Hospital Tongji Medical College Huazhong University of Science and Technology Department of Infectious Diseases Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

The pandemic of the coronavirus disease 2019(COVID-19) has become a global public health crisis. The symptoms of COVID-19 range from mild to severe, but the physiological changes associated with COVID-19 are barely understood. In this study, we performed targeted metabolomic and lipidomic analyses of plasma from a cohort of patients with COVID-19 who had experienced different symptoms. We found that metabolite and lipid alterations exhibit apparent correlation with the course of disease in these patients, indicating that the development of COVID-19 affected their whole-body metabolism. In particular, malic acid of the TCA cycle and carbamoyl phosphate of the urea cycle result in altered energy metabolism and hepatic dysfunction, respectively. It should be noted that carbamoyl phosphate is profoundly down-regulated in patients who died compared with patients with mild symptoms. And, more importantly, guanosine monophosphate(GMP), which is mediated not only by GMP synthase but also by CD39 and CD73, is significantly changed between healthy subjects and patients with COVID-19, as well as between the mild and fatal cases. In addition, dyslipidemia was observed in patients with ***, the disturbed metabolic patterns have been found to align with the progress and severity of COVID-19. This work provides valuable knowledge about plasma biomarkers associated with COVID-19 and potential therapeutic targets, as well as an important resource for further studies of the pathogenesis of COVID-19.

关键词： SARS-Co V-2 COVID-19 metabolome lipidome