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Functional analysis of the ABA-responsive protein family in ABA and stress signal transduction in Arabidopsis

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Chinese Science Bulletin 2013年第31期58卷 3721-3730页

作者： LIU LingYun LI Na yao chunpeng MENG SaSa SONG chunpeng State Key Laboratory of Cotton Biology Laboratory of Plant Stress Biology Department of Biology Henan University Zhengzhou Shuqing Medical College Faculty of Basic Medicine

The phytohormone abscisic acid(ABA)plays an important role in plant growth and development,for example in seed dormancy and germination,as well as in plant responses to environmental stresses,such as drought and high *** studies have shown that ABA regulates the expression of genes with an ABA-responsive element(ABRE)and their corresponding physiological *** analysis identified a GRAM domain-containing gene family that has a multiple ABRE cis-element,which was termed the ABA-responsive protein(ABR)*** analyze the function of the ABR family,we identified homozygous T-DNA insertion mutants and constructed abr1,2,3 double mutants and triple *** abr1,abr2 and abr3single mutants showed a normal phenotype;however,the germination of seeds of the double mutants and triple mutant were insensitive to ABA,NaCl,mannitol and ***1-GFP was distributed as a punctate structure in the cytosol and may be localized in the endomembrane *** ABR2-GFP and ABR3-GFP proteins localized in the *** addition,ABR1,ABR2 and ABR3 were expressed in various tissues,and could be induced by several abiotic stresses,especially by *** expressions of these genes were significantly suppressed in aba2,abi1 and abi2 null *** results suggested that the ABR family may act downstream of ABI1 and ABI2 in the ABA signal transduction process in plants.

关键词： ABA调节基因家族非生物胁迫蛋白定位信号转导生理反应拟南芥植物生长发育

High-density genetic mapping identified a major locus for environmental sex expression in pumpkin(Cucurbita moschata Duch.)

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Horticultural Plant Journal 2022年第5期8卷 593-601页

作者： Hafiz Muhammad Khalid Abbas Hexun Huang Tingquan Wu Rui Wang Hu Du Sen Lu Shudan Xue chunpeng yao Qingmin Jin Yujuan Zhong Guangdong Key Laboratory for New Technology Research of Vegetables Vegetable Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China

Long-day length and high temperature inhibit sex expression in pumpkin(Cucurbita moschata Duch.),and therefore directly impact the production *** this study,female flowering patterns in photoperiod-insensitive(PPIS)and photoperiod-sensitive(PPS)germplasms differed significantly in a moderately long day and high temperature ***,both germplasms exhibited a similar response in short day with either low temperature or high temperature *** sensitivity led to this difference in sex expression between the *** the traits of 1st female flowering node(FFFN)and number of female flowers(NFF),high-density linkage map construction and quantitative trait locus(QTL)mapping were performed using SLAF-seq technology and 162 F_(2) individuals generated from PPIS and *** total,4655 SLAFs were selected and mapped on 20 linkage groups(LGs).The total map length was 2502.01 cM with an average interval distance of 0.75 *** QTLs for both FFFN and NFF were detected on LG6 with intervals of 7.89 and 17.67 cM and PVE values of 30.5%and 22.9%,*** analyses of the major locus for FFFN revealed 73 protein-coding *** them,4 were related to sex expression,photoperiod flowering,and hormone *** InDel(insertion and deletion)marker partially correlated with FFFN of the F_(2) population was also *** study identified the QTL for the sex expression response to environmental factors using the high-density linkage *** identified candidate genes and markers will provide useful information about the molecular interaction between the environment and sex expression and for marker-assisted selection of pumpkin environment-insensitive resources.

关键词： Pumpkin Photoperiod sensitivity Sex expression High-density genetic map SLAF-seq QTL mapping

Suppression of edr2-mediated powdery mildew resistance, cell death and ethylene-induced senescence by mutations in ALD1 in Arabidopsis

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Journal of Genetics and Genomics 2011年第4期38卷 137-148页

作者： Haozhen Nie Yingying Wu chunpeng yao Dingzhong Tang State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

EDR2 is a negative regulator of the defense response and cell death in ***-of-function of EDR2 leads to enhanced resistance to powdery *** identify new components in the EDR2 signal transduction pathway,mutations that suppress edr2 resistant phenotypes were *** mutants,edts5-1,edts5-2 and edts5-3（edr two suppressor 5）,were *** EDTS5 gene was identified by map-based cloning and previously was shown to encode an aminotransferase（ALD1）.Therefore we renamed these three alleles ald1-10,ald1-11 and ald1-12,*** in ALD1 suppressed all edr2-mediated phenotypes,including powdery mildew resistance,programmed cell death and ethylene-induced *** of hydrogen peroxide in edr2 was also suppressed by ald1 *** expression of defense-related genes was up-regulated in the edr2 mutant,and the up-regulation of those genes in edr2 was suppressed in the edr2/ald1 double *** ald1 single mutant displayed delayed ethylene-induced *** addition,ald1 mutation suppressed edr1-mediated powdery mildew resistance,but could not suppress the edr11edr2 double-mutant *** data demonstrate that ALD1 plays important roles in edr2-mediated defense responses and senescence,and revealed a crosstalk between ethylene and salicylic acid signaling mediated by ALD1 and EDR2.

关键词： ALD1 Ambidopsis Defense signaling EDR2 Powdery mildew Salicylic acid

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邵雍先后天概念与中医先后天之本论的形成

中国哲学史 2022年第5期 110-115页

作者：姚春鹏曲阜师范大学政治与公共管理学院哲学系

先天、后天是源自《易传》的一对范畴,在邵雍“先天象数学”中得到了发展,并影响到明代之后的中医学。自明代医家孙一奎开始以先天、后天范畴为依据,以“人人有一太极”为思想资源,探究人体之太极——先天命门对生命活动的根源意义,发... 详细信息

先天、后天是源自《易传》的一对范畴,在邵雍“先天象数学”中得到了发展,并影响到明代之后的中医学。自明代医家孙一奎开始以先天、后天范畴为依据,以“人人有一太极”为思想资源,探究人体之太极——先天命门对生命活动的根源意义,发展了中医藏象学理论。经过孙一奎的“肾间动气”命门说,赵献可的“命门无形之火,在两肾有形之中”,张介宾的“命门总主乎两肾,而两肾皆属于命门”;最后由李中梓以“一点元阳则寓于两肾之间,是为命门”,将肾与命门合一而概括为肾为先天之本,又提出脾为后天之本,完成了中医先后天之本的理论创建,邵雍先天学本体论为贯穿其中的哲学理据。

关键词：邵雍先天后天命门

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理学“理气”论与后期中医学

中国哲学史 2009年第1期 57-62页

作者：姚春鹏曲阜师范大学政治与社会发展学院哲学系

理学理气论对后期中医学的发展产生了重要影响。由于理学的启导,后期中医学强调对医理的研究。但是由于中医学与理学属于不同的两门学科,二者在深层上对理气的理解是不同的。医学所探讨的理是医理、人体生命科学之理,而理学所探讨的理... 详细信息

理学理气论对后期中医学的发展产生了重要影响。由于理学的启导,后期中医学强调对医理的研究。但是由于中医学与理学属于不同的两门学科,二者在深层上对理气的理解是不同的。医学所探讨的理是医理、人体生命科学之理,而理学所探讨的理主要是道德之理、社会之理。由于理学的价值立场,在理气观上存在着褒理贬气的倾向;而中医学恰好相反,是重理宝气。在理气关系上,中医学继承了朱子的理气不分先后论,主理气合一之学。朱子的理本论在认识论上发展为中医学对理的重视。在学理上中医学重理宝气,理气并重,而理学的褒理贬气导出的伦理学上的理欲之辨对中医的养生、发病与治疗思想产生了重要影响,强调养生与养德的必然联系。

关键词：理学理气后期中医学

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张介宾医学自然观初探

中国哲学史 2019年第4期 107-113页

作者：姚春鹏曲阜师范大学政治与公共管理学院哲学系

张介宾的医学自然观继承了自周敦颐以来确立的太极、阴阳、五行生化万物的基本模式,并且结合医学实践的需要做了深化发展。其太极并不是朱熹的理本论的太极而是生成论的理气合一的太极。其阴阳是形上与形下合一的阴阳,形上的阴阳阐明了... 详细信息

张介宾的医学自然观继承了自周敦颐以来确立的太极、阴阳、五行生化万物的基本模式,并且结合医学实践的需要做了深化发展。其太极并不是朱熹的理本论的太极而是生成论的理气合一的太极。其阴阳是形上与形下合一的阴阳,形上的阴阳阐明了万物生化的根本机理,形下的阴阳为万物体象之总纲,为医学实践提供了以简御繁的认识论基础。其五行论阐明了五行与阴阳的内在联系,克服了传统五行生克理论简单化的弊病,发展出生克反用,五行互藏的复杂理论。比较而言。更能够反映复杂的自然现象与规律。张介宾的医学自然观虽然总体上缺乏哲学理论的原创性,但在局部的创见也是比较突出的,对中国古代医学和科学的发展有所贡献。

关键词：张介宾太极阴阳五行

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象思维的基本特点

中医杂志 2014年第18期55卷 1531-1534页

作者：姚春鹏曲阜师范大学政治与公共管理学院山东省日照市烟台路80号276826

象思维是与概念思维相对的、完全不同的一种思维形式,也是中医学理论体系建构和临床实践的思维基础。象思维的基本特征包括有象性、整体动态性、非逻辑性和直觉体悟性四个方面,这些特性决定了中医理论不同于西医学的独特性。

关键词：象思维有象性整体性动态性逻辑性直觉性体悟性

Visual Image Processing of Humanoid Go Game Robot Based on OPENCV

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Visual Image Processing of Humanoid Go Game Robot Based on O...

第32届中国控制与决策会议

作者： Yongjie Gui Yanyan Wu Yajie Wang chunpeng yao School of Computer Science Shenyang Aerospace University Engineering Training Center Shenyang Aerospace University

The visual system is a key part of the research on humanoid *** at the chessboard information extraction of Go robots,a python-based chessboard recognition method is *** transformation is used to correct the image,and binarization is used to enhance the *** processing techniques such as color space transformation,threshold segmentation,high-pass filtering,and Huff transform are used to detect,locate,and segment chess pieces in a chess *** show that the method can locate and segment chess pi eces accurately and has a high recognition rate.

关键词： Projection transformation High-pass filtering Hough detection Checkerboard recognition

来源： cnki会议评论

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象思维与六腑命名

中医杂志 2019年第22期60卷 1891-1894页

作者：姚春鹏姚丹曲阜师范大学政治与公共管理学院

与五脏一样,六腑之命名也决定于象思维,胃、肠(大肠、小肠)、膽(胆)、膀胱、焦(三焦的声旁不仅表示读音,也是意义的主要来源。古人根据解剖与经验观察获得了六腑结构和功能认知,运用象思维取象比类的方法确定了六腑之名。明乎此才可能... 详细信息

与五脏一样,六腑之命名也决定于象思维,胃、肠(大肠、小肠)、膽(胆)、膀胱、焦(三焦的声旁不仅表示读音,也是意义的主要来源。古人根据解剖与经验观察获得了六腑结构和功能认知,运用象思维取象比类的方法确定了六腑之名。明乎此才可能深化象思维与中医理论建构关系的理解。

关键词：象思维胃肠膽膀胱焦取象比类