High-density genetic mapping identified a major locus for environmental sex expression in pumpkin(Cucurbita moschata Duch.)

作     者：Hafiz Muhammad Khalid Abbas Hexun Huang Tingquan Wu Rui Wang Hu Du Sen Lu Shudan Xue Chunpeng Yao Qingmin Jin Yujuan Zhong Hafiz Muhammad Khalid Abbas;Hexun Huang;Tingquan Wu;Rui Wang;Hu Du;Sen Lu;Shudan Xue;Chunpeng Yao;Qingmin Jin;Yujuan Zhong

作者机构：Guangdong Key Laboratory for New Technology Research of VegetablesVegetable Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2022年第8卷第5期

页      面：593-601页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by Key Realm R&D Program of Guangdong Province(Grant No.2020B020220003) the National Natural Science Foundation of China(Grant No.31601748) the Agricultural competitive industry discipline team building project of Guangdong Academy of Agricultural Sciences(Grant No.202103TD) 

主　　题：Pumpkin Photoperiod sensitivity Sex expression High-density genetic map SLAF-seq QTL mapping 

摘      要：Long-day length and high temperature inhibit sex expression in pumpkin(Cucurbita moschata Duch.),and therefore directly impact the production *** this study,female flowering patterns in photoperiod-insensitive(PPIS)and photoperiod-sensitive(PPS)germplasms differed significantly in a moderately long day and high temperature ***,both germplasms exhibited a similar response in short day with either low temperature or high temperature *** sensitivity led to this difference in sex expression between the *** the traits of 1st female flowering node(FFFN)and number of female flowers(NFF),high-density linkage map construction and quantitative trait locus(QTL)mapping were performed using SLAF-seq technology and 162 F_(2) individuals generated from PPIS and *** total,4655 SLAFs were selected and mapped on 20 linkage groups(LGs).The total map length was 2502.01 cM with an average interval distance of 0.75 *** QTLs for both FFFN and NFF were detected on LG6 with intervals of 7.89 and 17.67 cM and PVE values of 30.5%and 22.9%,*** analyses of the major locus for FFFN revealed 73 protein-coding *** them,4 were related to sex expression,photoperiod flowering,and hormone *** InDel(insertion and deletion)marker partially correlated with FFFN of the F_(2) population was also *** study identified the QTL for the sex expression response to environmental factors using the high-density linkage *** identified candidate genes and markers will provide useful information about the molecular interaction between the environment and sex expression and for marker-assisted selection of pumpkin environment-insensitive resources.