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饲料添加谷氨酸对草鱼生长、抗氧化能力和肌肉品质性状的影响

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水生生物学报 2024年第9期48卷 1509-1518页

作者：杨婉愉巫丽云张月星韩冬张志敏刘昊昆金俊琰杨云霞朱晓鸣解绶启浙江海洋大学国家海洋设施养殖工程技术研究中心舟山316022 中国科学院水生生物研究所淡水生态与生物技术国家重点实验室武汉430072

实验以草鱼(Ctenopharyngodon idella)为研究对象,探究饲料中添加谷氨酸对草鱼生长性能、肌肉品质和血浆抗氧化能力的影响。研究配制3组等氮等脂实验饲料:对照饲料(Con)、在对照饲料中添加质量分数0.8%和1.6%的L-谷氨酸(分别为0.8%GLU组... 详细信息

实验以草鱼(Ctenopharyngodon idella)为研究对象,探究饲料中添加谷氨酸对草鱼生长性能、肌肉品质和血浆抗氧化能力的影响。研究配制3组等氮等脂实验饲料:对照饲料(Con)、在对照饲料中添加质量分数0.8%和1.6%的L-谷氨酸(分别为0.8%GLU组和1.6%GLU组)。将540尾初始质量为(37.0±0.2)g的草鱼分为3个处理,分别投喂1种实验饲料,每个处理设3个重复网箱,每个网箱放养60尾鱼,养殖周期85d。研究结果发现,饲料中添加谷氨酸组草鱼的生长性能与对照组无显著差异;与对照相比,1.6%GLU组草鱼的背肌肌肉间隙减小,肌纤维密度显著增加(P<0.05);与对照相比,0.8%GLU组和1.6%GLU组草鱼背肌的硬度、咀嚼性、黏附性、内聚性和回复性显著增加,0.8%HYP组肌肉弹性显著增加(P<0.05);饲料中添加谷氨酸组草鱼血浆总胆固醇含量显著下降(P<0.05);1.6%GLU组草鱼血浆碱性磷酸酶AKP活性显著增加(P<0.05);0.8%GLU组和1.6%GLU组草鱼血浆谷丙转氨酶活性显著下降(P<0.05);0.8%GLU组和1.6%GLU组草鱼血浆超氧化物歧化酶SOD活性和总抗氧化能力T-AOC均显著增加(P<0.05)。综上,饲料中添加谷氨酸有助于改善草鱼肌肉品质性状,提高草鱼血浆抗氧化能力。

关键词：谷氨酸肌肉品质抗氧化能力草鱼

Review:Actin-based dynamics during spermatogenesis and its significance

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第7期8卷 498-506页

作者： XIAO Xiang yang wan-xi The Sperm Laboratory Institute of Cell Biology and Genetics School of Life Sciences Zhejiang University Hangzhou 310058 China

Actin can be found in all kinds of eukaryotic cells, maintaining their shapes and motilities, while its dynamics in sperm cells is understood less than their nonmuscle somatic cell counterparts. Spermatogenesis is a complicated process, resulting in the production of mature sperm from primordial germ cell. Significant structural and biochemical changes take place in the seminif-erous epithelium of the adult testis during spermatogenesis. It was proved that all mammalian sperm contain actin, and that F-actin may play an important role during spermatogenesis, especially in nuclear shaping. Recently a new model for sperm head elonga-tion based on the acrosome-acroplaxome-manchette complex has been proposed. In Drosophila, F-actin assembly is supposed to be very crucial during individualization. In this mini-review, we provide an overview of the structure, function, and regulation characteristics of actin cytoskeleton, and a summary of the current status of research of actin-based structure and movement is also provided, with emphasis on the role of actins in sperm head shaping during spermiogenesis and the cell junction dynamics in the testis. Research of the Sertoli ectoplasmic specialization is in the spotlight, which is a testis-specific actin-based junction very important for the movement of germ cells across the epithelium. Study of the molecular architecture and the regulating mechanism of the Sertoli ectoplasmic specialization has become an intriguing field. All this may lead to a new strategy for male infertility and, at the same time, a novel idea may result in devising much safer contraception with high efficiency. It is hoped that the advances listed in this review would give developmental and morphological researchers a favorable investigating outline and could help to enlarge the view of new strategies and models for actin dynamics during spermatogenesis.

关键词： Testis Actin Spermatogenesis Nuclear shaping Sertoli cell

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注水增湿+高能级强夯在湿陷性黄土中的应用

葛洲坝集团科技 2024年第1期 69-72页

作者：雷建龙赵群熊凯万阳中国葛洲坝集团建设工程有限公司云南昆明650217

XLJD项目土质为湿陷性黄土,土体含水量较低,天然含水率在3.9%~8.4%之间,在XLJD单点夯试验区开展15000kN·m直接高能级强夯增湿与18000kN·m直接高能级强夯增湿试验对比研究,以期探索出一条适宜于兰州地区湿陷性黄土区增湿联合高能级强... 详细信息

XLJD项目土质为湿陷性黄土,土体含水量较低,天然含水率在3.9%~8.4%之间,在XLJD单点夯试验区开展15000kN·m直接高能级强夯增湿与18000kN·m直接高能级强夯增湿试验对比研究,以期探索出一条适宜于兰州地区湿陷性黄土区增湿联合高能级强夯地基处理的施工工艺。

关键词：湿陷性黄土高能级强夯增湿施工

阴阳调理灸联合有氧运动对老年单纯性肥胖的疗效分析

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中国疗养医学 2024年第2期33卷 67-70页

作者：王旭红牛丹妹杨万芳杨常青河南省漯河市医学高等专科学校第三附属医院

目的探讨阴阳调理灸联合有氧运动对老年单纯性肥胖的治疗效果。方法选取2022年8月至2023年7月于河南省漯河市医学高等专科学校第三附属医院内分泌科就诊的106例单纯性肥胖患者为研究对象。将患者按照随机数字表分为对照组和观察组，... 详细信息

目的探讨阴阳调理灸联合有氧运动对老年单纯性肥胖的治疗效果。方法选取2022年8月至2023年7月于河南省漯河市医学高等专科学校第三附属医院内分泌科就诊的106例单纯性肥胖患者为研究对象。将患者按照随机数字表分为对照组和观察组，每组各53例。对照组给予有氧运动干预，观察组给予阴阳调和灸联合有氧运动。两组均干预12周，比较两组治疗前后肥胖指标[体质量、腰围、体质指数(BMI)、体脂百分比(F%)]、血清淋巴管功能相关因子[血管内皮生长因子C(VEGF-C)、delta样配体4(DLL4)及肾上腺髓质素(ADM)]，并比较两组临床疗效及治疗前后痰湿体质量表转化分。结果与治疗前相比，两组患者治疗后肥胖指标均下降(P<0.05)，且观察组患者低于对照组(P<0.05)。与治疗前相比，两组患者治疗后血清肠淋巴管功能相关因子VEGF-C、DLL4及ADM水平均下降(P<0.05)，且观察组患者低于对照组(P<0.05)。观察组总有效率高于对照组(94.34%VS 81.13%,P<0.05)。与治疗前相比，两组患者治疗后痰湿体质量表转化分均下降(P<0.05)，且观察组低于对照组(P<0.05)。结论对单纯性肥胖患者在有氧运动基础上联合阴阳调和灸可进一步改善肥胖指标及肠淋巴管功能相关因子水平，具有较好的临床效果，其患者痰湿偏颇体质得到显著改善。

关键词：单纯性肥胖阴阳调理灸有氧运动肥胖指标

Positive adaptation of Salix eriostachya to warming in the treeline ecotone,East Tibetan Plateau

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Journal of Mountain Science 2017年第2期14卷 346-355页

作者： XIAN Jun-ren CHEN Guo-peng LIU Yin-zhan XU Xiao-xun yang Zhan-biao yang wan-qin School of Environmental Sciences Sichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130China Institute of forestry Sciences Bailongjiang Forestry Management Bureau of Gansu ProvinceLanzhou 730070China Key Laboratory of Plant Stress Biology College of Life SciencesHenan UniversityKaifeng 475004China Institute of Ecological Forestry Sichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130China

Understanding of treeline ecotone ecophysiological adaptation to climate warming is still very limited. Furthermore, it is difficult to predict which plant species could dominate in the future. For this reason, a study was conducted in the treeline ecotone, East Tibetan Plateau to detect the adaptation of the dwarf willow(Salix eriostachya) to experimental warming. Compared to ambient conditions, the experimental warming advanced the bud break by 12 days, delayed the leaf abscission by20 days, and prolonged the growing period by 28 *** also increased photosynthesis(47%), number of leaves(333%), leaf area(310%), and carbon sequestration of the dwarf willow. Experimental warming did not affect carbon use efficiency, but decreased water use efficiency *** warming enhanced the clonal ramets of Salix eriostachya(+ 3.7 shrubs m-2). The frequent air temperature fluctuations had minor effect on Salix eriostachya. Based on these findings, we highlighted that Salix eriostachya could dominate in the community treeline ecotone of east Tibetan Plateau in the future climate warming scenario.

关键词： Dwarf willow Experimental warming Ecophysiological adaptation Dominating Treeline

Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells

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World Journal of Stem Cells 2023年第5期15卷 369-384页

作者： Jia-Qi Zhou Hao-yang wan Zi-Xuan wang Nan Jiang Division of Orthopaedics and Traumatology Department of OrthopaedicsNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina

Mesenchymal stem cells(MSCs),distributed in many tissues in the human body,are multipotent cells capable of differentiating in specific *** is usually considered that the differentiation process of MSCs depends on specialized external stimulating factors,including cell signaling pathways,cytokines,and other physical *** findings have revealed other underrated roles in the differentiation process of MSCs,such as material morphology and *** relevant achievements have substantially advanced the applicability of MSCs,some of these regulatory mechanisms still need to be better ***,limitations such as long-term survival in vivo hinder the clinical application of MSCs *** review article summarizes current knowledge regarding the differentiation patterns of MSCs under specific stimulating factors.

关键词： Mesenchymal stem cells Differentiation Osteogenic Chondrogenic Literature review

Novel, Green, Simple and Uncatalyzed Route for High Yield Preparation of Benzaldehyde Glycol Acetal

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Chemical Research in Chinese Universities 2011年第5期27卷 799-802页

作者： TANG Jia SONG Ji-yuan wanG Ge yang Mu GUO wan-chun School of Material Science and Engineering University of Science and Technology Beijing Beij＇ing 100083 P. R. China

A simple approach for the condensation of benzaldehyde with ethylene glycol was adopted without employing any catalyst. The study reveals that factors including temperature, molar ratio of reactants, and the water removal significantly influence the conversion ratio of benzaldehyde. The results indicate that the use of optimal reaction conditions such as temperature and water removing exhibits more pronounced effects on the conversion ratio of benzaldehyde compared with the use of catalysts. In a word, a green and simple method for the condensation of aldehyde and menthandiol has been devised and investigated.

关键词： Condensation Benzaldehyde Ethylene glycol

A novel cellulose hydrogel prepared from its ionic liquid solution

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Chinese Science Bulletin 2009年第9期54卷 1622-1625页

作者： LI Lu LIN ZhangBi yang Xiao wan ZhenZhen CUI ShuXun State Key Laboratory of Polymer Materials Engineering Polymer Research Institute Sichuan University Chengdu 610065 China

A novel cellulose hydrogel is prepared by regenerating cellulose from its ionic liquid solution. The transparency cellulose hydrogel presents a good chemical stability and an acceptable mechanical property. This non-t... 详细信息

关键词：再生纤维素离子液体水凝胶制备纤维素凝胶化学稳定性生物相容性力学性能

Effects of mTOR-STAT3 on the migra=tion and invasion abilities of hepatoma cell and mTOR-STAT3 expression in liver cancer

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Asian Pacific Journal of Tropical Medicine 2014年第5期7卷 368-372页

作者： Xia Pu Qing-Xi Guo Han-An Long Cheng-wan yang Department of Pathology Affiliated Hospital of Luzhou Medical College

Objective:To investigate the effects of mT0R-STAT3 pathway on the invasion and migration of hepatoma ***:mTOR and STAT3 expresssion in the hepatocellular carcinoma cell line HepG2 and normal liver cell line L02 were detected by reverse transcription PCR(RTPCR) and western *** migration and invasion abilities of cells and expression of STAT3were detected by scratch adhesion test and transwell migration assays,after siRNA transfection blocking mTOR expression of HepG2 ***:The HepG2 cells expression is higher compared with normal cells L02 *** blotting assay showed the mTOR expression was blocked,while STAT3 expression was also decreased,after the siRNA transfection of *** migration(scratch adhesion test) and invasion(transwell assays) abilities of HepG2 cells which the mTOR expression was blocked by siRNA interference were significantly decreased(P<0.05).Conclusion:mT0RSTAT3 expression in hepatoma cells HepG2 was significantly higher than that in normal liver *** blocking can reduce the expression of STAT3,which is also closely related to the invasion and metastasis of liver cancer cells.

关键词： Liver cancer mTOR/STAT3 Invasion Metastasis