Effects of mTOR-STAT3 on the migra=tion and invasion abilities of hepatoma cell and mTOR-STAT3 expression in liver cancer

作     者：Xia Pu Qing-Xi Guo Han-An Long Cheng-Wan Yang 

作者机构：Department of PathologyAffiliated Hospital of Luzhou Medical College 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2014年第7卷第5期

页      面：368-372页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Key Subject of Scientific Research of the Education Department of Sichuan Province(Project Number:13ZA0233) 

主　　题：Liver cancer mTOR/STAT3 Invasion Metastasis 

摘      要：Objective:To investigate the effects of mT0R-STAT3 pathway on the invasion and migration of hepatoma ***:mTOR and STAT3 expresssion in the hepatocellular carcinoma cell line HepG2 and normal liver cell line L02 were detected by reverse transcription PCR(RTPCR) and western *** migration and invasion abilities of cells and expression of STAT3were detected by scratch adhesion test and transwell migration assays,after siRNA transfection blocking mTOR expression of HepG2 ***:The HepG2 cells expression is higher compared with normal cells L02 *** blotting assay showed the mTOR expression was blocked,while STAT3 expression was also decreased,after the siRNA transfection of *** migration(scratch adhesion test) and invasion(transwell assays) abilities of HepG2 cells which the mTOR expression was blocked by siRNA interference were significantly decreased(P0.05).Conclusion:mT0RSTAT3 expression in hepatoma cells HepG2 was significantly higher than that in normal liver *** blocking can reduce the expression of STAT3,which is also closely related to the invasion and metastasis of liver cancer cells.