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CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean

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The Crop Journal 2024年第4期12卷 1093-1101页

作者： Le Gao Lijun Xie Yanmin Xiao Xinge Cheng Ruosi Pu Ziheng Zhang Yu Liu shaopei Gao Zilong Zhang Haoran Qu Haijian Zhi Kai Li MARA Key Laboratory of Biology and Genetics Improvement of Soybean Zhongshan Biological Breeding Laboratory(ZSBBL)National Innovation Platform for Soybean Breeding and Industry-Education IntegrationState Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationNanjing Agricultural UniversityNanjing 210095JiangsuChina Department of Horticulture Beijing Vocational College of AgricultureBeijing 102442China College of Agronomy&Biotechnology China Agricultural UniversityBeijing 100193China College of Bioscience and Biotechnology Shenyang Agricultural UniversityShenyang 110866LiaoningChina

Soybean mosaic virus(SMV),an RNA virus,is the most common and destructive pathogenic virus in soybean *** newly developed CRISPR/Cas immune system has provided a novel strategy for improving plant resistance to viruses;hence,this study aimed to engineer SMV resistance in soybean using this ***,multiple sgRNAs were designed to target positive-and/or negative-sense strands of the SMV HC-Pro ***,the corresponding CRISPR/CasRx vectors were constructed and transformed into *** inoculation with SMV,39.02%,35.77%,and 18.70%of T_(1)plants were confirmed to be highly resistant(HR),resistant(R),and mildly resistant(MR)to SMV,respectively,whereas only 6.50%were identified as susceptible(S).Additionally,qRT-PCR and DAS-ELISA showed that,both at 15 and 30 d post-inoculation(dpi),SMV accumulation significantly decreased or was even undetectable in HR and R plants,followed by MR and S ***,the expression level of the CasRx gene varied in almost all T_(1)plants with different resistance level,both at 15 and 30 ***,when SMV resistance was evaluated in the T_(2)generation,the results were similar to those recorded for the T_(1)*** findings provide new insights into the application of the CRISPR/CasRx system for soybean improvement and offer a promising alternative strategy for breeding for resistance to biotic stress that will contribute to the development of SMV-immune soybean germplasm to accelerate progress towards greater soybean crop productivity.

关键词： Soybean RNA virus Soybean mosaic virus Resistance CRISPR/CasRx

IbNIEL-mediated degradation of IbNAC087 regulates jasmonic acid-dependent salt and drought tolerance in sweet potato

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Journal of Integrative Plant Biology 2024年第2期66卷 176-195页

作者： Xu Li Zhen Wang Sifan Sun Zhuoru Dai Jun Zhang Wenbin Wang Kui Peng Wenhao Geng Shuanghong Xia Qingchang Liu Hong Zhai shaopei Gao Ning Zhao Feng Tian Huan Zhang Shaozhen He Key Laboratory of Sweet Potato Biology and Biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis&Utilization and Joint Laboratory for International Cooperation in Crop Molecular BreedingMinistry of EducationCollege of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Sanya 572025China State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterKey Laboratory of Biology and Genetic Improvement of MaizeBeijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Sweet potato(Ipomoea batatas[L.]Lam.)is a crucial staple and bioenergy *** abiotic stress tolerance holds significant importance in fully utilizing marginal *** processes regulate abiotic stress responses,yet the molecular regulatory mechanisms in sweet potato remain *** this study,a NAC(NAM,ATAF1/2,and CUC2)transcription factor,IbNAC087,was identified,which is commonly upregulated in salt-and drought-tolerant *** of IbNAC087 increased salt and drought tolerance by increasing jasmonic acid(JA)accumulation and activating reactive oxygen species(ROS)scavenging,whereas silencing this gene resulted in opposite ***-rich IbNAC087-OE(overexpression)plants exhibited more stomatal closure than wild-type(WT)and IbNAC087-Ri plants under NaCl,polyethylene glycol,and methyl jasmonate ***087 functions as a nuclear transcriptional activator and directly activates the expression of the key JA biosynthesisrelated genes lipoxygenase(IbLOX)and allene oxide synthase(IbAOS).Moreover,IbNAC087 physically interacted with a RING-type E3 ubiquitin ligase NAC087-INTERACTING E3 LIGASE(IbNIEL),negatively regulating salt and drought tolerance in sweet *** ubiquitinated IbNAC087 to promote 26S proteasome degradation,which weakened its activation on IbLOX and *** findings provide insights into the mechanism underlying the IbNIEL-IbNAC087 module regulation of JA-dependent salt and drought response in sweet potato and provide candidate genes for improving abiotic stress tolerance in crops.

关键词： abiotic tolerance IbNAC087 IbNIEL JA sweet potato

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铁路货车钩舌检修数字孪生车间的研究与应用

计算机集成制造系统 2024年第7期30卷 2474-2485页

作者：杨康吴少培董志宇李国芳丁旺才兰州交通大学机电工程学院甘肃兰州730070

为了实现对铁路货车检修车间的实时透明管控,提高车间管理的信息化和智能化水平。首先,基于数字孪生技术,提出一种数字孪生检修车间六维模型,介绍了系统核心功能和开发流程,并详细阐述了数据采集及交换、三维可视化场景构建,被检对象生... 详细信息

为了实现对铁路货车检修车间的实时透明管控,提高车间管理的信息化和智能化水平。首先,基于数字孪生技术,提出一种数字孪生检修车间六维模型,介绍了系统核心功能和开发流程,并详细阐述了数据采集及交换、三维可视化场景构建,被检对象生成等全要素精准建模过程。其次,基于分布式MQTT构架的数据采集及交换的方法,建立多源异构数据流转节点的状态矩阵,利用嵌入式设备、采集卡、传感器,逻辑控制器与车间管控系统进行数据交换,结合检修车间设备、物料、产线等检修全过程动态模型,构建了虚实交互映射的检修车间数字孪生系统。最后,通过基于数据挖掘的钩舌检修跟踪、检修工艺甘特图等技术输出,展示了数字孪生关键技术在钩舌智能检修中的应用场景,验证了所提方法的有效性,同时为数字孪生在智能轨道交通中的应用提供参考。

关键词：数字孪生检修车间实时信息铁路货车

可见光拓展InP/InGaAs宽光谱红外探测器

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红外与激光工程 2015年第11期44卷 3177-3180页

作者：史衍丽郭骞李龙邓功荣杨绍培范明国刘文波北方夜视科技集团有限公司云南昆明650223

由于标准InP/In0.53Ga0.47As短波红外探测器的响应波段为0.87~1.7μm,在高性能夜视中具有重要的应用。为了进一步利用夜天光在可见光区间的辐射能量,需要将InP/In0.53Ga0.47As短波探测器的光谱响应拓展到可见光,从而实现包含可见光和短... 详细信息

由于标准InP/In0.53Ga0.47As短波红外探测器的响应波段为0.87~1.7μm,在高性能夜视中具有重要的应用。为了进一步利用夜天光在可见光区间的辐射能量,需要将InP/In0.53Ga0.47As短波探测器的光谱响应拓展到可见光,从而实现包含可见光和短波波段的宽光谱探测。通过特殊的材料设计和背减薄工艺,成功研制了可见光拓展的320×256 InP/InGaAs宽光谱红外探测器。采用增加滤光片的方法完成了器件在可见光、短波的成像演示,结果表明：目标在可见光、短波波段呈现出不同的特征信息,而不加滤光片的可见光拓展InP/InGaAs宽光谱红外探测器则探测到两个波段的信息,既包含目标的可见光信息同时也具有短波信息,从而实现了可见/短波双波段探测的效果,可显著提升对目标的探测能力。

关键词： InP/In0.53Ga0.47As 光谱响应可见光拓展可见/短波双波段探测

InP/InGaAs探测器氮化硅钝化膜的ICPCVD生长技术

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红外技术 2019年第6期41卷 511-514页

作者：龚燕妮杨文运杨绍培范明国褚祝军北方夜视技术股份有限公司云南昆明650223 北方夜视科技集团有限公司云南昆明650223

采用ICPCVD工艺对InP/InGaAs探测器进行SiNx薄膜钝化,讨论了不同气体比例、射频功率和反应压力对SiNx薄膜应力和致密性的影响。初步得到了低应力、良好致密性的SiNx薄膜沉积工艺。利用此工艺制作的InP/InGaAs探测器在偏压为-10mV的暗电... 详细信息

采用ICPCVD工艺对InP/InGaAs探测器进行SiNx薄膜钝化,讨论了不同气体比例、射频功率和反应压力对SiNx薄膜应力和致密性的影响。初步得到了低应力、良好致密性的SiNx薄膜沉积工艺。利用此工艺制作的InP/InGaAs探测器在偏压为-10mV的暗电流比PECVD工艺制作的器件降低了一个数量级,为4.4×10^-8A。

关键词： InP/InGaAs探测器 ICPCVD 氮化硅

基于STC8A8K64S4A12的农用无人机电流表系统研究

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信息记录材料 2022年第6期23卷 42-47页

作者：安红恩杨少沛许强黄河交通学院机电工程学院河南焦作454950

为提升农作物植保作业中无人机的电源容量、续航能力和通信效果,以STC8A8K64S4A12处理器为平台,集成数据采集模块(CS5460A)和通信模块(RS485),利用单片机C语言和汇编语言指令,设计了一种基于嵌入式微处理器为核心的农用无人机电流表系统... 详细信息

为提升农作物植保作业中无人机的电源容量、续航能力和通信效果,以STC8A8K64S4A12处理器为平台,集成数据采集模块(CS5460A)和通信模块(RS485),利用单片机C语言和汇编语言指令,设计了一种基于嵌入式微处理器为核心的农用无人机电流表系统,并使用多功能校准仪、信号发生器、示波器等仪器就输入数据和显示数据的稳定性和可靠性进行试验验证,并对引起测量误差的起因进行了研究分析。通过无人机飞行实验,验证了此电流表系统的稳定性和可靠性,解决了无人机电源容量校小、续航能力差和通信效果不佳等问题。

关键词：农用无人机电流表系统嵌入式微处理器

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无人机空中充电智能系统研究

信息记录材料 2022年第5期23卷 18-20页

作者：安红恩杨少沛许强黄河交通学院机电工程学院河南焦作454950

无人机的续航能力始终是影响无人机发展的一个重要因素,为解决这一问题提出了空中充电系统设计。无人机空中无线充电系统设计主要包括空中充电的设计,无线电能发送模块、无线电能接收模块的设计,电源检测系统的设计,抗干扰系统设计,通... 详细信息

无人机的续航能力始终是影响无人机发展的一个重要因素,为解决这一问题提出了空中充电系统设计。无人机空中无线充电系统设计主要包括空中充电的设计,无线电能发送模块、无线电能接收模块的设计,电源检测系统的设计,抗干扰系统设计,通信识别模块设计等。在磁共振无线充电的基础上,论述了S-S WPT系统结构和LCC-S拓扑结构,为无人机空中充电提供参考依据,同时探讨空中充电系统的运行体系结构,总结出不同体系结构的特点和使用方式,具有一定的应用价值。

关键词：无人机空中充电智能

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基于融合稀疏模型的引导单幅图像去雨算法

传感器与微系统 2020年第4期39卷 148-151,160页

作者：王科平蔡凯利杨少沛杨艺河南理工大学电气工程与自动化学院河南焦作454150 黄河交通学院河南焦作454950

针对现有单幅图像去雨算法存在雨线残留较明显或背景过度平滑的问题,本文提出融合稀疏模型的引导单幅图像去雨算法。通过交替方向乘子法分别对融合解析和综合稀疏模型学习出含部分雨线残留的背景图和雨线图;采用具有较好保边平滑性的引... 详细信息

针对现有单幅图像去雨算法存在雨线残留较明显或背景过度平滑的问题,本文提出融合稀疏模型的引导单幅图像去雨算法。通过交替方向乘子法分别对融合解析和综合稀疏模型学习出含部分雨线残留的背景图和雨线图;采用具有较好保边平滑性的引导滤波得到最终去雨图。实验结果表明:所提算法在峰值信噪比和结构相似性方面均比近三年典型对比算法有所提高,且有效去除雨线的同时能较大程度地保留图像背景细节信息。

关键词：单幅图像去雨解析稀疏模型综合稀疏模型交替方向乘子法引导滤波

FUZZY ECONOMIC ASSESSMENT FOR OFFSHORE OIL FIELDS

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China Ocean Engineering 1990年第1期5卷 97-108页

作者： Lin, shaopei Sun, Qiliang yang, Xiaofeng Shanghai Jiao Tong Univ Shanghai China

It is understood that economic assessment is a key issue in the development of offshore oil field. In view of uncertainties in exploration, engineering investment etc. the capital investment will take serious risk, especially for small marginal blocks. In order to investigate the relationships between main economic indices and uncertain factors, the upper and lower fuzzy bounds as well as the tolerance are presented in this paper based on the concept of fuzzy mathematics. Quantizing those uncertainties of concerned factors, the risk of investment can then be identified through conventional cash flow table and sensitivity analysis, consequently, the risk can be reduced through careful handling of the uncertainties. As an example, the economic analysis of a reusable platform in a marginal offshore oil field is presented, which shows the process of fuzzy assessment in exposing severe marginality of the field and the performance of discounted cash flow rate of return by the fluctuation of uncertainties.

关键词： Economics Analysis Mathematical Techniques Fuzzy Sets