CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean

作     者：Le Gao Lijun Xie Yanmin Xiao Xinge Cheng Ruosi Pu Ziheng Zhang Yu Liu Shaopei Gao Zilong Zhang Haoran Qu Haijian Zhi Kai Li Le Gao;Lijun Xie;Yanmin Xiao;Xinge Cheng;Ruosi Pu;Ziheng Zhang;Yu Liu;Shaopei Gao;Zilong Zhang;Haoran Qu;Haijian Zhi;Kai Li

作者机构：MARA Key Laboratory of Biology and Genetics Improvement of SoybeanZhongshan Biological Breeding Laboratory(ZSBBL)National Innovation Platform for Soybean Breeding and Industry-Education IntegrationState Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationNanjing Agricultural UniversityNanjing 210095JiangsuChina Department of HorticultureBeijing Vocational College of AgricultureBeijing 102442China College of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193China College of Bioscience and BiotechnologyShenyang Agricultural UniversityShenyang 110866LiaoningChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2024年第12卷第4期

页      面：1093-1101页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by grants from National Natural Science Foundation of China(32001571) R&D Program of Beijing Municipal Education Commission(KM202212448003,KM202312448004) Science and Technology Innovation Project of Beijing Vocational College of Agriculture(XY-YF-22-02) Zhongshan Biological Breeding Laboratory(ZSBBL-KY2023-03) China Agriculture Research System of MOF and MARA(CARS-04) Jiangsu Collaborative Innovation Center for Modern Crop Production(JCICMCP) Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP) 

主　　题：Soybean RNA virus Soybean mosaic virus Resistance CRISPR/CasRx 

摘      要：Soybean mosaic virus(SMV),an RNA virus,is the most common and destructive pathogenic virus in soybean *** newly developed CRISPR/Cas immune system has provided a novel strategy for improving plant resistance to viruses;hence,this study aimed to engineer SMV resistance in soybean using this ***,multiple sgRNAs were designed to target positive-and/or negative-sense strands of the SMV HC-Pro ***,the corresponding CRISPR/CasRx vectors were constructed and transformed into *** inoculation with SMV,39.02%,35.77%,and 18.70%of T_(1)plants were confirmed to be highly resistant(HR),resistant(R),and mildly resistant(MR)to SMV,respectively,whereas only 6.50%were identified as susceptible(S).Additionally,qRT-PCR and DAS-ELISA showed that,both at 15 and 30 d post-inoculation(dpi),SMV accumulation significantly decreased or was even undetectable in HR and R plants,followed by MR and S ***,the expression level of the CasRx gene varied in almost all T_(1)plants with different resistance level,both at 15 and 30 ***,when SMV resistance was evaluated in the T_(2)generation,the results were similar to those recorded for the T_(1)*** findings provide new insights into the application of the CRISPR/CasRx system for soybean improvement and offer a promising alternative strategy for breeding for resistance to biotic stress that will contribute to the development of SMV-immune soybean germplasm to accelerate progress towards greater soybean crop productivity.