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Re-expression of DIRAS3 and P53 induces apoptosis and impaired autophagy in head and neck squamous cell carcinoma

Military Medical research 2021年第2期8卷 148-161页

作者： Zhe Liu Douglas R.Hurst Xing qu Li-Guang lu chen-Zhou Wu Yu-Yu Li Yi Li State Key Laboratory of Oral Diseases&national Clinical research {2. for Oral Diseases West China Hospital of StomatologySichuan UniversityChengdu 610041China Department of Head and Neck Oncology West China Hospital of StomatologySichuan UniversityChengdu 610041China Department of Pathology University of Alabama at BirminghamBirminghamAL 35216USA Department of Evidence Based Stomatology West China Hospital of StomatologySichuan UniversityChengdu 610041China.

Background:p53 and DIRAS3 are tumor suppressors that are frequently silenced in *** this study,we sought to determine whether the concurrent re-expression of p53 and DIRAS3 could effectively induce head and neck squamous cell carcinoma(HNSCC)cell ***:CAL-2. and SCC-2. cells were treated with Ad-DIRAS3 and rAd-p53 to induce re-expression of DIRAS3 and p53 *** effects of DIRAS3 and p53 re-expression on the growth and apoptosis of HNSCC cells were examined by TUNEL assay,flow cytometric analysis and *** effects of DIRAS3 and p53 re-expression on Akt phosphorylation,oncogene expression,and the interaction of 4 E-BP1 with eIF4 E were determined by real-time PCR,Western blotting and immunoprecipitation *** ability of DIRAS3 and p53 re-expression to induce autophagy was evaluated by transmission electron microscopy,LC3 fluorescence microscopy and Western *** effects of DIRAS3 and p53 re-expression on HNSCC growth were evaluated by using an orthotopic xenograft mouse ***:TUNEL assay and flow cytometric analysis showed that the concurrent re-expression of DIRAS3 and p53 significantly induced apoptosis(Pduced cell cycle arrest(Pducer and activation of transcription 3(STAT3)and upregulated p2.WAF1/CIP1 and Bax(Pduced the expression of c-Myc,cyclin D1,vascular endothelial growth factor(VEGF),fibroblast growth factor(FGF),epidermal growth factor receptor(EGFR)and Bcl-2.P2.%,P<

关键词： DIRAS3 p53 Apoptosis Autophagy Head and neck squamous cell carcinoma

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Impacts of regional land-use patterns on ecosystem services in the typical agro-pastoral ecotone of northern China

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Ecosystem Health and Sustainability 2022年第1期8卷 534-553页

作者： Huimin chen Yu Zhao Xiao Fu Mingfang Tang Mingjie Guo Shiqi Zhang Yu Zhu Laiye qu gang Wu State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina China IPPR International Engineering Co. LtdBeijingChina

A comprehensive understanding of the spatial-temporal evolution and driving forces on ecosystem services(ES)is essential for the agro-pastoral ecotone’s ecological security in northern ***,the land-use pattern(luLC)agglomeration with spatial differentiation in the pastoral and agricultural areas has been rarely *** distinct luLC(1980-2.18)in Chifeng as an example,we compared four crucial categories of ESs with *** SEM,we further contrasted the effects of several variables on regional ES variations in pastoral-dominated(North)and agriculture-dominated(South)regions,*** revealed the conversion between forest and grassland oriented the luLC transformation in the *** contrast,human-activitiy-oriented land tended to occupy environmentally sensitive places in the *** ES variations were supplied with the North outperforming the South when soil conservation was *** for the impacts of regional ES variations,the natural and luLC policies both showed positive effects,whereas the anthropogenic factors showed positive in the North,which was negative in the ***,the ecologicallymaintained-dominant and ecologically-restored-dominant strategies should be separately adopted in the North and *** study provided appropriate regional ecological management suggestions for balancing the luLC-driven conflicts between ecological protection and regional development.

关键词： Distinct land use/land cover(luLC)patterns Spatialtemporal evolution of ecosystem services(ESs) Agro-pastoral ecotone of northern China InVEST models Structural equation modeling(SEM)

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Site-specific determination of TTR-related functional peptides by using scanning tunneling microscopy

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Nano research 2018年第1期11卷 577-585页

作者： Lanlan Yu Yongfang Zheng JingXu Fuyang qu Yuchen Lin Yimin Zou Yanlian yang Sally L. Gras chen Wang CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Center for Excellence in Nanoscience CAS Center for Excellence in Brain Science National Center for Nanoscience and Technology Beijing 100190 China Department of Chemical and Biomolecular Engineering and Bio2. Molecular Science and Biotechnology Institute The University of Melbourne Parkville Victoria 3010 Australia Department of Chemistry Tsinghua University Beijing 100084 China University of Chinese Academy of Sciences Beijing 100049 China

For the design and optimization of functional peptides, unravelling the structures of individual building blocks as well as the properties of the ensemble is paramount. TI＇R1, derived from human transthyretin, is a fibril-forming peptide implicated in diseases such as familial amyloid polyneuropathy and senile systemic amyloidosis. The functional peptide TTR1-RGD, based on a TFR1 scaffold, was designed to specifically interact with cells. Here, we used scanning tunneling microscopy （STM） to analyze the assembly structures of TTRl-related peptides with both the reverse sequence and the modified forward sequence. The site- specific analyses show the following： i） The TIR1 peptide is involved in assembly, nearly covering the entire length within the ordered [3-sheet structures, ii） For TTR1-RGD peptide assemblies, the TTR1 motif forms the ordered [3-sheet while the RGDS motif adopts a flexible conformation allowing it to promote cell adhesion. The key site is clearly identified as the linker residue Gly13. iii） Close inspection of the forward and reverse peptide assemblies show that in spite of the difference in chemistry, they display similar assembling characteristics, illustrating the robust nature of these peptides, iv） Glycine linker residues are included in the ^-strands, which strongly suggests that the sequence could be optimized by adding more linker residues. These garnered insights into the assembled structures of these peptides help unravel the mechanism driving peptide assemblies and instruct the rational design and optimization of sequence- programmed peptide architectures.

关键词： TTR1 functional peptide key site scanning tunnelingmicroscopy (STM) optimization

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Hexagonal boron nitride nanosheet for effective ambient N2.fixation to NH3

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Nano research 2019年第4期12卷 919-924页

作者： Ya Zhang Huitong du Yongjun Ma Lei Ji Haoran Guo Ziqi Tian Hongyu chen Hong Huang Guanwei Cui Abdullah M. Asiri Fengli qu Liang chen Xuping Sun Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China Chengdu 610054 China College of Chemistry and Chemical Engineering Qufu Normal University Qufu 273165 China Analytical and Test center Southwest University of Science and Technology Mianyang 621010 China Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences Ningbo 315201 China College of Chemistry Chemical Engineering and Materials Science Shandong Normal University Jinan 250014 China Chemistry Department Faculty of Science & Center of Excellence for Advanced Materials Research King Abdulaziz University P.O. Box 80203 Jeddah 21589 Saudi Arabia

Industrial production of NH3 from N2.and H2.significantly relies on Haber-Bosch process,which suffers from high energy consume and CO2.*** a sustainable and environmentally-benign alternative process,electrochemical artificial N2.fixation at ambient conditions,however,is highly required efficient *** this study,we demonstrate that hexagonal boron nitride nanosheet (h-BNNS) is able to electrochemically catalyze N2.to *** acidic solution,h-BNNS catalyst attains a high NH3 formation rate of 2..4 μg·h-1·*** a high Faradic efficiency of 4.7% at-0.75 V *** hydrogen electrode,with excellent stability and *** functional theory calculations reveal that unsaturated boron at the edge site can activate inert N2.molecule and significantly reduce the energy barrier for NH3 fonmation.

关键词： boron nitride nanosheet N2 reduction reaction NH3 electrosynthesis ambient conditions density functional theory

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Surface Br?nsted-Lewis dual acid sites for high-efficiency dinitrogen photofixation in pure water

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Journal of Energy Chemistry 2022年第4期67卷 824-830页

作者： Cai chen Jiewei chen Zhiyuan Wang Fei Huang Jian {2. Yunteng qu Kuang Liang Xiao Ge {2.gang Wang Hui Zhang Yuen Wu College of Chemistry and Chemical Engineering Southwest Petroleum University Department of Chemistry Guangdong Provincial Key Laboratory of Catalysis Southern University of Science and Technology School of Chemistry and Materials Science iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China Shenyang national Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences

As is known to all, nitrogen not only plays an important role in the industrial development of human society but also plays an important part in the proteins that constitute the essence of life[1]. In 1910, the Haber-... 详细信息

关键词： Photocatalytic nitrogen fixation Brønsted-Lewis dual acid sites Surface-modified TiO2 Surface states F and H atoms

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Rapid phenotypic evolution with shallow genomic differentiation during early stages of high elevation adaptation in Eurasian Tree Sparrows

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national Science Review 2020年第1期7卷 113-127页

作者： Yanhua qu Chunhai chen Ying Xiong Huishang She Yong E Zhang Yalin cheng Shane du Bay Dongming Li Per G P Ericson Yan Hao Hongyuan Wang Hongfeng Zhao {2. Song Hailin Zhang Ting yang Chi Zhang Liping Liang Tianyu Wu Jinyang Zhao Qiang Gao Weiwei Zhai Fumin Lei Key Laboratory of Zoological Systematics and Evolution Institute of Zoology Chinese Academy of Sciences BGI Genomics BGI-Shenzhen University of Chinese Academy of Sciences center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences Committee on Evolutionary Biology University of Chicago Life Sciences Section Integrative Research Center Field Museum of Natural History Key Laboratory of Animal Physiology Biochemistry and Molecular Biology of Hebei Province College of Life Sciences Hebei Normal University Department of Bioinformatics and Genetics Swedish Museum of Natural History College of Life Sciences Shaanxi Normal University China national Gene Bank BGI-Shenzhen Human Genetics Genome Institute of Singapore Agency for Science Technology and Research

Known as the ‘third polar region’, the Qinghai-Tibet Plateau represents one of the harshest highland environments in the world and yet a number of organisms thrive there. Previous studies of birds, animals and humans have focused on well-differentiated populations in later stages of phenotypic divergence. The adaptive processes during the initial phase of highland adaptation remain poorly understood. We studied a human commensal, the Eurasian Tree Sparrow, which has followed human agriculture to the Qinghai-Tibet Plateau. Despite strong phenotypic differentiation at multiple levels, in particular in muscle-related phenotypes, highland and lowland populations show shallow genomic divergence and the colonization event occurred within the past few thousand years. In a one-month acclimation experiment investigating phenotypic plasticity, we exposed adult lowland tree sparrows to a hypoxic environment and did not observe muscle changes. Through population genetic analyses, we identified a signature of polygenic adaptation, whereby shifts in allele frequencies are spread across multiple loci, many of which are associated with muscle-related processes. Our results reveal a case of positive selection in which polygenic adaptation appears to drive rapid phenotypic evolution, shedding light on early stages of adaptive evolution to a novel environment.

关键词： denovo genome early stage adaptation high elevation muscle phenotypes polygenic adaptation regulatory evolution

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Expression and inhibitory activity analysis of a 2.-kD Bowman-Birk protease inhibitor in rice

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Chinese Science Bulletin 2006年第1期51卷 54-62页

作者： chen Jun MAO Shengji XlE yang CAO Zhongren ZHANG Yan LIU jing chen Zhangliang qu Lijia GU Hongya Peking-Yale Joint center for Plant Molecular Genetics and Agro-Biotechnology National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China national Plant Gene research {2. (beijing) Beijing 100101 China

Rice Bowman-Birk inhibitors (RBBI), with one (8 kD) or two homologous domains (16 kD), were found to be effective trypsin inhibitors in vitro. In this study, we demonstrate that the 2.-kD protein corresponding to the three-domain RBBI indeed ex- ists in rice in planta, and that the RBBIs are regulated by development and wounding. We also found by inhibitory activity assay that the 3:13 disulfide bond, but not the 4:5 disulfide bond, suppresses the tryp- sin-inhibitory activity, and the D3 domain of RBBI3-1 has no inhibitory activity against trypsin, chymotryp- sin, paparin or subtilisin. Mutation analyses showed that conversion from Lys to Leu or Tyr in the N-terminal P1 site in D1 domain did not create chy- motrypsin-inhibitory activity, suggesting that the structure of the reactive loop in D1 domain hinder the new inhibitory specificity at P1 site, and the chy- motrypsin-inhibitory activity might need the participa- tion of other structures, e.g. 3:13 disulfide bond.

关键词：水稻 RBBI 双硫键胰凝乳蛋白酶胰岛素

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The functional analysis of transiently upregulated miR-101 suggests a “braking” regulatory mechanism during myogenesis

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Science China(Life Sciences) 2021年第10期64卷 1612-1623页

作者： Shurong Liu Shujuan Xie Huafeng chen Bin Li Zhirong chen Yeya Tan Jianhua {2. Lingling Zheng Zhendong Xiao Qi Zhang Lianghu qu MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Vaccine research Institute of Sun Yat-sen University the Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Cell-Gene Therapy Translational Medicine research center the Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China

Skeletal muscle differentiation is a highly coordinated process that involves many cellular signaling pathways and microRNAs(miRNAs).A group of muscle-specific miRNAs has been reported to promote myogenesis by suppressing key signaling pathways for cell ***,the functional role and regulatory mechanism of most non-muscle-specific miRNAs with stage-specific changes during differentiation are largely ***,we describe the functional characterization of miR-101a/b,a pair of non-muscle-specific miRNAs that show the largest change among a group of transiently upregulated miRNAs during myogenesis in C2.12.*** overexpression of miR-101a/b inhibits myoblast differentiation by suppressing the p38/MAPK,Interferon Gamma,and Wnt pathways and enhancing the C/EBP ***2.,a key protein in the p38/MAPK pathway,was identified as a direct target of miR-101a/***,we found that the long non-coding RNA(lncRNA)Malat1,which promotes muscle differentiation,interacts with miR-101a/b,and this interaction competes with Mef2. mRNA to relieve the inhibition of the p38/MAPK pathway during *** results uncovered a“braking”role in differentiation of transiently upregulated miRNAs and provided new insights into the competing endogenous RNA(ceRNA)regulatory mechanism in myoblast differentiation and myogenesis.

关键词： miR-101a/b p38/MAPK signaling pathway Mef2. Malat1 skeletal muscle differentiation

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Nickle-cobalt alloy nanocrystals inhibit activation of inflammasomes

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national Science Review 2023年第8期10卷 211-221页

作者： Jun Lin Liang Dong Yi-Ming Liu Yi Hu chen Jiang Ke Liu Liu Liu Yong-Hong Song Mei Sun Xing-cheng Xiang Kun qu {2. lu Long-Ping Wen Shu-Hong Yu Department of Neurosurgery The First Affiliated Hospital of USTC School of Basic Medical SciencesDivision of Molecular Medicine Department of Chemistry Institute of Biomimetic Materials&Chemistry Division of Nanomaterials&Chemistry Hefei National Research Center for Physical Sciences at the Microscale University of Science and Technology of China The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC) Chinese Academy of Sciences Anhui Province Key Laboratory of Advanced Catalytic Materials and Reaction Engineering School of Chemistry and Chemical EngineeringHefei University of Technology The WUT-AMU Franco-Chinese Institute Wuhan University of Technology Institute of Artificial Intelligence Hefei Comprehensive National Science Center

Activation of inflammasomes—immune system receptor sensor complexes that selectively activate inflammatory responses—has been associated with diverse human diseases, and many nanomedicine studies have reported that structurally and chemically diverse inorganic nanomaterials cause excessive inflammasome activation. Here, in stark contrast to reports of other inorganic nanomaterials, we find that nickel-cobalt alloy magnetic nanocrystals(NiCo NCs) actually inhibit activation of NLRP3, NLRC4 and AIM2.inflammasomes. We show that NiCo NCs disrupt the canonical inflammasome ASC speck formation process by downregulating the lncRNA Neat1, and experimentally confirm that the entry of NiCo NCs into cells is required for the observed inhibition of inflammasome activation. Furthermore, we find that NiCo NCs inhibit neutrophil recruitment in an acute peritonitis mouse model and relieve symptoms in a colitis mouse model, again by inhibiting inflammasome activation. Beyond demonstrating a highly surprising and apparently therapeutic impact for an inorganic nanomaterial on inflammatory responses, our work suggests that nickel-and cobalt-containing nanomaterials may offer an opportunity to design anti-inflammatory nanomedicines for the therapeutics of macrophage-mediated diseases.

关键词： inflammasome nickel-cobalt nanoparticle Neat1 colitis NLRP3

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Effect of Zn on mechanical and corrosion properties of Mg-Sc-Zn alloys

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Journal of Materials Science & Technology 2023年第27期158卷 31-42页

作者： wenjun Ci Xianhua chen Yue Sun Xu Dai Guanzheng Zhu Di Zhao Fusheng Pan International Joint Laboratory for Light Alloys(MOE) College of Materials Science and EngineeringChongqing UniversityChongqing 400045China national Engineering research {2. for Magnesium Alloys Chongqing UniversityChongqing 400045China State Key Laboratory of Power Transmission Equipment&System Security and New Technology Chongqing UniversityChongqing 400044China School of Mechanical Engineering Suzhou University of Science and TechnologySuzhou 215009China

The effect of different Zn concentrations(0 wt.%,1 wt.%,3 wt.%,and 6 wt.%)on the microstructure,cor-rosion property,and mechanical property of Mg-0.3Sc-x Zn(x=0 wt.%,1 wt.%,3 wt.%,and 6 wt.%)alloys was ***,MSZ1 alloy exhibits the highest corrosion resistance(0.194 mm/y)and appropriate mechanical properties with an ultimate tensile strength of 2.8 MPa and elongation of 19%.The superior corrosion resistance of Mg-0.3Sc-1Zn alloys is attributed to the homogeneous volta-potential distribution and the dense corrosion product *** the increase in zinc content,the strength and plasticity of Mg-0.3Sc-x Zn alloys(x=0 wt.%,1 wt.%,3 wt.%,6 wt.%)improved to some *** precipitated ScZn phase plays the role of the second phase strengthening,which enables MSZ6 to obtain the maximum tensile ***,the ScZn phase with low volta potential intensifies the galvanic corrosion,re-sulting in the decline of the corrosion performance.

关键词： High corrosion resistance Mechanical performance Mg-Sc-Zn alloys SKPFM Corrosion product film

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