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Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole(Cynoglossus semilaevis)

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Zoological Research 2022年第3期43卷 319-330页

作者： Yi-Fang Lu Qian Liu Kai-Qiang Liu hong-yan wang Cheng-Hua Li Qian wang Chang-Wei Shao School of Marine Sciences Ningbo UniversityNingboZhejiang 315211China Key Lab of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoShandong 266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and TechnologyQingdaoShandong 266000China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China

Chinese tongue sole(Cynoglossus semilaevis)is an economically important marine fish species with a ZZ/ZW sex determination mechanism,which can be influenced by *** splicing(AS)is an important mechanism regulating the expression of genes related to sex determination and gonadal differentiation,but has rarely been reported in *** this study,to explore the molecular regulatory mechanisms of sex determination and gonadal differentiation,we combined isoform and RNA sequencing(Iso-Seq and RNA-Seq)to perform transcriptome profiling of male and female gonads in *** total,81883 and 32341 full-length transcripts were obtained in males and females,respectively.A total of 8279 AS genes were identified,including 2639 genes showing differential AS(DAS)between males and *** intersecting DAS genes and differentially expressed genes(DEGs)were enriched in the meiotic cell cycle pathway,and genes related to gonadal differentiation,such as esrrb and wt1a,were found to have sex-specific ***,this study revealed AS events in the gonadal transcriptomes of male and female ***,described the characteristics of active transcription in the testes,and identified candidate genes for studying the regulatory mechanisms of AS during gonadal differentiation.

关键词： Gonadal differentiation Alternative splicing Sex-specific splicing Cynoglossus semilaevis Iso-Seq RNA-Seq

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Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro

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World Journal of Stem Cells 2024年第2期16卷 163-175页

作者： yan Ren yan-Ni Cui hong-Wei wang The Second Clinical Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases in Shanxi ProvinceTaiyuan 030001Shanxi ProvinceChina

BACKGROUND In vitro expansion to increase numbers of hematopoietic stem cells(HSCs)in cord blood could improve clinical efficacy of this vital ***(NAM)can promote HSC expansion ex vivo,but its effect on hematopoietic stem and progenitor cells(HSPCs,CD34^(+)CD38)and functional subtypes of HSCs-shortterm repopulating HSCs(ST-HSCs,CD34^(+)CD38CD45RACD49f^(+))and long-term repopulating HSCs(LT-HSCs,CD34^(+)CD38CD45RACD49f^(+)CD90^(+))is not yet *** a sirtuin 1(SIRT1)inhibitor,NAM participates in regulating cell adhesion,polarity,migration,proliferation,and ***,SIRT1 exhibits dual effects by promoting or inhibiting differentiation in different tissues or *** propose that the concentration of NAM may influence proliferation,differentiation,and SIRT1 signaling of *** To evaluate the effects and underlying mechanisms of action of different concentrations of NAM on HSC proliferation and *** CD34^(+)cells were purified from umbilical cord blood using MacsCD34 beads,and cultured for 10-12 d in a serum-free medium supplemented with cytokines,with different concentrations of NAM added according to experimental *** cytometry was used to detect phenotype,cell cycle distribution,and apoptosis of the cultured ***-time polymerase chain reaction was used to detect the transcription levels of target genes encoding stemness-related factors,che mokines,components of hypoxia pathways,and antioxidant ***-dihydro-fluorescein diacetate probes were used to evaluate intracellular production of reactive oxygen species(ROS).Determination of the effect of different culture conditions on the balance of cytokine by cytometric bead *** Compared with the control group,the proportion and expansion folds of HSPCs(CD34^(+)CD38)incubated with 5 mmol/L or 10 mmol/L NAM were significantly increased(all P<0.05).The ST-HSCs ratio and fold expansion of the 5 mmol/L NAM group were significantly

关键词： Hematopoietic stem cells Nicotinamide Concentration Proliferation Differentiation Sirtuin 1

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Nondestructive Quantitative Analysis of Cofrel Medicines by Double ANN-NIR Spectroscopy

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Chinese Chemical Letters 2006年第9期17卷 1209-1212页

作者： Ming yang LIU Yu MENG Jun Feng LI Hai Tao ZHANG hong yan wang College of Chemistry Jilin University Changchun 130012 Pfizer Pharmaceuticals Limited Co. Dalian 116600

In this paper, a double artificial neural network （DANN） algorithm was used to parse near infrared （NIR） reflectance spectrum of Cofrel medicines. The contents of benproperine phosphate, which is the effective ingredient in Cofrel medicines, were accurately nondestructive quantitatively predicted. Compared the results with those of HPLC, the relative errors （RE %） were less than 0.18%. The analytical results could be applied to qualitative control of Cofrel medicines.

关键词： Double ANN NIR spectroscopy nondestructive quantitative analysis Cofrel.

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Beneficial effect of probiotics supplements in reflux esophagitis treated with esomeprazole: A randomized controlled trial

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World Journal of Gastroenterology 2019年第17期25卷 2110-2121页

作者： Qing-Hua Sun hong-yan wang Shi-Dong Sun Xin Zhang Han Zhang Department of Gastroenterology Peking University Third Hospital Department of Gastroenterology PKUCare Luzhong Hospital

BACKGROUND Reflux esophagitis(RE) is a common digestive disorder, and its frequent recurrences cause significant physical pain and are financially burdensome to patients. However, studies on the natural history of treated RE are few. Although proton pump inhibitors(PPIs) as the first-line treatment provide notable symptomatic relief, disordered gut microbiota has been observed among PPI users. Probiotics are commonly administered to patients to regulate the disordered intestinal *** To evaluate the therapeutic effects in RE patients treated with a combination of esomeprazole and probiotics [Bacillus subtilis(B. subtilis) and Enterococcus faecium(E. faecium)].METHODS One hundred and thirty-four RE patients were randomized into two groups of 67 subjects each. The probiotics group was administered with esomeprazole 20 mg b.i.d. and live combined B. subtilis and E. faecium enteric-coated capsules 500 mg t.i.d. for eight weeks; the placebo group was administered with esomeprazole 20 mg b.i.d. and placebo for eight weeks. Subsequently, 12-wk follow-up was carried out on patients who achieved both endoscopic and clinical cure. Endoscopy,reflux diagnostic questionnaire(RDQ), gastrointestinal symptom rating scale(GSRS), and lactulose hydrogen breath test were performed to evaluate the therapeutic effects. A difference of P 0.05). However, the GSRS diarrhea syndrome score was decreased significantly in the probiotics group(P = 0.002),and the small intestinal bacterial overgrowth negative rate in the probiotics group was significantly higher than that in the placebo group(P = 0.002). Of 114 endoscopically and clinically cured patients, 96 completed the follow-up. The logrank test showed that the time to relapse was shorter

关键词： Proton pump inhibitors Probiotics Small intestinal bacterial overgrowth Reflux esophagitis Relapse

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Rapid determination of C1^- by light-absorption ratio variation approach using Cl^--eosin Y-Ag^+ adsorptive precipitation

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Chinese Chemical Letters 2008年第5期19卷 569-572页

作者： Jing Bai hong yan wang hong Wen Gao State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and Engineering Tongji University Shanghai 200092 China

The adsorptive precipitation between eosin Y （EY） and AgCl colloids at pH 3.73 caused the sensitive color change of the solution. The reaction mechanism between EY and AgCl was analyzed and this reaction was used fo... 详细信息

关键词： Determination of chloride Eosin Y Adsorptive precipitation Light-absorption ratio variation approach

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Anaphylaxis effect and substance basis of honeysuckle extract

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Chinese Herbal Medicines 2021年第3期13卷 403-409页

作者： Yu-fei Feng Guo-zhao Qin Zhong-xu Jing yan-hong wang yan-yan Zhou Heilongjiang University of Chinese Medicine Harbin 150040China Heilongjiang Province Traditional Chinese Medicine Administration Harbin 150040China

Objective:To explore the anaphylaxis effect and anaphylaxis substances of ***:Rat peritoneal mast cells(PMC)were separated and purified,the cells were incubated with compound 48/80(0.02 g/L),physiological saline and honeysuckle extract(120 g/L)at 37°C for 0,15,30,45 and 60 *** were observed by optical microscope and transmission electron *** V positive cell rate was detected by flow cytometry to reflect the degranulation rate of *** rats were supplied with honeysuckle extract by intravenous injection at a dose of 2.25 g/*** administration,different parameters were analyzed,including the symptoms,histamine(HIS)and tryptase(MCT)levels,which were determined to explore the effect of *** analysis was used to calculate the relationships between the peaks and the pharmacological effects to explore potentially anaphylactoid ***:The percentage of Annxin V positive cells and the degranulation ratio were markedly elevated in PMC treated with honeysuckle extract for more than 15 min(P<0.05).HIS and MCT level were significantly elevated after injection of honeysuckle extract for more than 15 *** of PMC and systemic symptoms were also changed compared with the controlled group(P<0.05).Regression analysis was used to calculate the relationship between peaks and pharmacological effects,and to determine peaks 7,10 and 13 as possible anaphylactoid ***:This study established a prospective method to clarify the anaphylactoid components of honeysuckle extract,which would provide guidance for screening anaphylactoid components in traditional Chinese medicine injections containing honeysuckle in the prescription.

关键词： anaphylaxis basis anaphylaxis effect honeysuckle extract spectrum-effect relationships

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Meiotic chromosome preparation techniques of pollen mother cells for laser micro-dissection in Populus spp.

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Forestry Studies in China 2010年第2期12卷 74-78页

作者： yanG hong-yan wang Jun KANG Xiang-yang National Engineering Laboratory for Tree Breeding Key Laboratory of Genetics and Breeding in Forest Trees and OrnamentalPlants of Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

In order to isolate meiotic chromosomes of Populus species meiotic chromosome preparation techniques of pollen mother cells for laser micro-dissection were studied. Pollen mother cells at diakinesis ofPopulus canadensis Moench were used as samples. Two methods were used to prepare meiotic chromosomes： in the first, cell suspensions were dropped on polyethylene-naphthalate or polyester membrane slides which had just been incubated at -20~C; in the second method, cell suspensions were also dropped on polyethylene-naphthalate or polyester membrane slides, but spread with the aid of high temperatures. The cells did not completely spread by the first method and chromosomes at diakinesis could not be individually distinguished. In contrast, well-spread diakinesis chromosomes were obtained by the second method, where chromosomes, connected with their nucleolus, were successfully isolated with the laser micro-dissection system. As well, we discuss the prospect of applications of laser micro-dissection in cytogeneties and molecular genetics in Populus species.

关键词： chromosomes diakinesis laser micro-dissection Populus spp. pollen mother cells

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Study on Multi-objective Optimization of Airbag Landing Attenuation System for Heavy Airdrop

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Defence Technology（防务技术） 2013年第4期9卷 237-241页

作者： hong-yan wang Huang-jie hong Jian-yang LI Qiang RUI Academy of Armored Force Engineering

A finite element model of vehicle and its airbag landing attenuation system is established and verified *** design cases are selected to constrain the airbag design for extreme landing conditions,while the height and width of airbag and the area of vent hole are chosen as design *** optimization is forced to compromise the design variables between the conflicting requirements of the two *** order to optimize the parameters of airbag,the multi-dimensional response surfaces based on extended Latin hypercube design and radial basis function are employed instead of the complex finite element *** optimal solution sets based on response surfaces are then obtained by multi-objective genetic *** results show the optimization method presented in this paper is a practical tool for the optimization of airbag landing attenuation system for heavy airdrop.

关键词：多目标优化系统衰减安全气囊空投着陆 Pareto最优解集有限元模型

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Anti-inflammatory effect and antihepatoma mechanism of carrimycin

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World Journal of Gastroenterology 2023年第14期29卷 2134-2152页

作者： Xiu-yan Li Yu-Ting Luo yan-hong wang Zhi-Xin yang Yu-Zhou Shang Qing-Xia Guan Key Laboratory of Basic and Application Research of Beiyao Heilongjiang University of Chinese MedicineHarbin 150040Heilongjiang ProvinceChina

BACKGROUND New drugs are urgently needed for the treatment of liver cancer, a feat that could be feasibly accomplished by finding new therapeutic purposes for marketed drugs to save time and costs. As a new class of national anti-infective drugs, carrimycin(CAM) has strong activity against gram-positive bacteria and no cross resistance with similar drugs. Studies have shown that the components of CAM have anticancer *** To obtain a deeper understanding of CAM, its distribution, metabolism and antiinflammatory effects were assessed in the organs of mice, and its mechanism of action against liver cancer was predicted by a network pharmacology *** In this paper, the content of isovaleryl spiramycin Ⅲ was used as an index to assess the distribution and metabolism of CAM and its effect on inflammatory factors in various mouse tissues and organs. Reverse molecular docking technology was utilized to determine the target of CAM, identify each target protein based on disease type, and establish a target protein-disease type network to ascertain the effect of CAM in liver cancer. Then, the key action targets of CAM in liver cancer were screened by a network pharmacology method, and the core targets were verified by molecular docking and visual *** The maximum CAM concentration was reached in the liver, kidney, lung and spleen 2.5 h after intragastric administration. In the intestine, the maximum drug concentration was reached 0.5 h after administration. In addition, CAM significantly reduced the interleukin-4(IL-4) levels in the lung and kidney and especially the liver and spleen;moreover, CAM significantly reduced the IL-1β levels in the spleen, liver, and kidney and particularly the small intestine and lung. CAM is predicted to regulate related pathways by acting on many targets,such as albumin, estrogen receptor 1, epidermal growth factor receptor and caspase 3, to treat cancer, inflammation and other *** We determined that C

关键词： Carrimycin Reverse molecular docking Network pharmacology Liver cancer Antiinflammatory Anti-hepatoma

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Simultaneous Determination of Gold and Platinum by Double Artificial Neural Network Analysis with Flow-injection Chemiluminescence

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Chinese Chemical Letters 2006年第10期17卷 1343-1346页

作者： Ming yang LIU Hai Tao ZHANG Jun Feng LI Shu Gui CHEN hong yan wang College of chemistry Jilin University Changchun 130012

A highly sensitive double artificial neural network （DANN） analysis with flow-injection chemiluminescence （FI-CL） has been developed to simultaneously determine the trace amounts of the gold and platinum in simula... 详细信息

关键词： Double artificial neural networks flow-injection chemiluminescence simultaneous determination gold and platinum.

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