Anaphylaxis effect and substance basis of honeysuckle extract

作     者：Yu-fei Feng Guo-zhao Qin Zhong-xu Jing Yan-hong Wang Yan-yan Zhou Yu-fei Feng;Guo-zhao Qin;Zhong-xu Jing;Yan-hong Wang;Yan-yan Zhou

作者机构：Heilongjiang University of Chinese MedicineHarbin 150040China Heilongjiang Province Traditional Chinese Medicine AdministrationHarbin 150040China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2021年第13卷第3期

页      面：403-409页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：project supported by the Heilongjiang University of Chinese Medicine Province and Excellent Youth Project of Natural Science Foundation of Heilongjiang Province(YQ2019H031) Postdoctoral Researchers Settled in Heilongjiang Scientific Research Startup Fund(2020) Outstanding Innovative Talents Project from Heilongjiang University of Chinese Medicine(2018) 

主　　题：anaphylaxis basis anaphylaxis effect honeysuckle extract spectrum-effect relationships 

摘      要：Objective:To explore the anaphylaxis effect and anaphylaxis substances of ***:Rat peritoneal mast cells(PMC)were separated and purified,the cells were incubated with compound 48/80(0.02 g/L),physiological saline and honeysuckle extract(120 g/L)at 37°C for 0,15,30,45 and 60 *** were observed by optical microscope and transmission electron *** V positive cell rate was detected by flow cytometry to reflect the degranulation rate of *** rats were supplied with honeysuckle extract by intravenous injection at a dose of 2.25 g/*** administration,different parameters were analyzed,including the symptoms,histamine(HIS)and tryptase(MCT)levels,which were determined to explore the effect of *** analysis was used to calculate the relationships between the peaks and the pharmacological effects to explore potentially anaphylactoid ***:The percentage of Annxin V positive cells and the degranulation ratio were markedly elevated in PMC treated with honeysuckle extract for more than 15 min(P0.05).HIS and MCT level were significantly elevated after injection of honeysuckle extract for more than 15 *** of PMC and systemic symptoms were also changed compared with the controlled group(P0.05).Regression analysis was used to calculate the relationship between peaks and pharmacological effects,and to determine peaks 7,10 and 13 as possible anaphylactoid ***:This study established a prospective method to clarify the anaphylactoid components of honeysuckle extract,which would provide guidance for screening anaphylactoid components in traditional Chinese medicine injections containing honeysuckle in the prescription.