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Carboxyl terminal of rhodopsin kinase is required for the phosphorylation of photo-activated rhodopsin

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Cell Research 1998年第4期8卷 303-310页

作者： YU QING MING ZHI JIE CHENG JIAN ZHAO TIAN HUA ZHOU ya lan wu lan MA GANG PEI(Shanghai Institute of Cell Biology, Chinese Academy of Science, 320 Yue yang Road, Shanghai 200031, China)(Department of Neurobiology, Shanghai Medical University,Shanghai Department of Neurobiology Shanghai Medical University Shanghai 200031 China

Human rhodopsin kinase (RK) and a carboxyl terminus-truncated mutant RK lacking the last 59 amino acids (RKC) were expressed in human embryonic kidney 293 cells to investigate the role of the carboxyl terminus of RK in recognition and phosphorylation of rhodopsin. RKC, like the wild-type RK, was detected in both plasma membranes and cytosolic fractions. The Cterminal truncated rhodopsin kinase was unable to phosphorylate photo-activated rhodopsin, but possesses kinase activity similar to the wild-type RK in phosphorylation of small peptide substrate. It suggests that the truncation did not disturb the gross structures of RK catalytic domain. Our results also show that RKC failed to translocate to photo-activated rod out segments. Taken together,our study demonstrate the carboxyl terminus of RK is required for phosphorylation of photo-activated rhodopsin and strongly indicate that carboxyl-terminus of RK may be involved in interaction with photo-activated rhodopsin.

关键词： Rhodopsin kinase phosphorylation fruncation

Induction of apoptosis and change of bcl-2 expression in macrophage Ana-1 cells by all-trans retinoic acid

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Cell Research 1996年第2期6卷 137-144页

作者： YIN DE LING XIU HAI REN SHI ZHONG BU ya lan wu LI ZHEN JIANG ZHI JIANG wu WEI HU GANG PEI (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China)(Correspondence: Dr. G. Pei, Shanghai Institute of Cell Biology Chine Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China.

Macrophage cells play an important role in the initiation and regulation of the immune response. All-trans retinoic acid (ATRA) and its natural and synthetic analogs (retinoids) affect a large number of biological *** , retinoids have been shown promise in the therapy and prevention of various cancers. However, many interesting questions related to the activities of retinoids remain to be answered: (Ⅰ) Molecular mechanisms by which retinoids exert their effects; (Ⅱ) why the clinical uses of retinoids give undesirable side effects of varying severity with a higher freqllency of blood system symptoms; (Ⅲ)little is known for its impacts on macrophage cells etc. We set up this experiment, therefore, to examine the apoptosis of ATRA on macrophage Ana-1 cell line. Apoptosis of the cells was quantitated, after staining cells with propidium iodide (PI), by both accounting nuclear condensation and flow cytometry. When the cells were treated with ATRA at or higher than 1 μM for more than 24 h, significant amount of the apoptotic cells was observed. Induction of apoptosis of Ana-1 cells by ATRA was in time- and dose-dependent manners, exhibiting the similar pattern as the apoptosis induced by actinomycin D (ACTD). ATRA treatment of Ana-1 cells also caused the changes of the mRNA levels of apoptosis-associated gene bcl-2, as detected by Northern blot analysis. The temporal changes of bcl-2 expression by ATRA was also parallel to that by ACTD. In conclusion,ATRA can induce apoptosis in macrophage cells, which may be helpful in understanding of immunological functions retinoids.

关键词： All-trans retinoic acid apoptosis Ana-1 cells bcl-2

Tuning the interfacial reaction environment via pH-dependent and induced ions to understand C–N bonds coupling performance in NO3- integrated CO2 reduction to carbon and nitrogen compounds over dual Cu-based N-doped carbon catalyst

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Journal of Energy Chemistry 2025年第1期100卷 273-285页

作者： Tianhang Zhou Chen Shen Zhenghao wu Xingying lan Yi Xiao College of Carbon Neutrality Future Technology State Key Laboratory of Heavy Oil Processing China University of Petroleum (Beijing) Institute of Materials Science TU Darmstadt Department of Chemistry Xi'an Jiaotong-Liverpool University Department of Mechanical and Mechatronics Engineering and Waterloo Institute of Nanotechnology University of Waterloo Waterloo

Dual atomic catalysts （DAC）,particularly copper （Cu2）-based nitrogen （N） doped graphene,show great potential to effectively convert CO2and nitrate （NO3-） into important industrial chemicals such as ethylene,glycol,acetamide,and urea through an efficient catalytical process that involves C–C and C–N ***,the origin of the coupling activity remained unclear,which substantially hinders the rational design of Cu-based catalysts for the N-integrated CO2reduction reaction （CO2RR）.To address this challenge,this work performed advanced density functional theory calculations incorporating explicit solvation based on a Cu2-based N-doped carbon (Cu2N6C10) catalyst for *** calculations are aimed to gain insight into the reaction mechanisms for the synthesis of ethylene,acetamide,and urea via coupling in the interfacial reaction *** to the sluggishness of CO2,the formation of a solvation electric layer by anions （F-,Cl-,Br-,and I-） and cations (Na+,Mg2+,K+,and Ca2+) leads to electron transfer towards the Cu *** process significantly accelerates the reduction of *** results reveal that*CO intermediates play a pivotal role in N-integrated ***,the Cu2-based N-doped carbon catalyst examined in this study has demonstrated the most potential for C–N coupling to *** findings reveal that through the process of a condensation reaction between*CO and NH2OH for urea synthesis,*NO3-is reduced to*NH3,and*CO2to*CCO at dual Cu atom *** dual-site reduction facilitates the synthesis of acetamide through a nucleophilic reaction between NH3and the ketene ***,we found that the I-and Mg2+ions,influenced by pH,were highly effective for acetamide and ammonia synthesis,except when F-and Ca2+were ***,the mechanisms of C–N bond formation were investigated via ab-initio molecular dynamics simulations,and we found that adjusting the micro-environment can change the dominant side reaction,shift

关键词： pH-dependent micro-environments C–N coupling Dual Cu-based nitrogen carbon catalysts Explicit solvation model Ion stabilizer AIMD

Erythropoietin Receptor Positive Circulating Progenitor Cells and Endothelial Progenitor Cells in Patients with Different Stages of Diabetic Retinopathy

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Chinese Medical Sciences Journal 2011年第2期26卷 69-76页

作者： Liu-mei Hu Xia Lei Bo Ma Yu Zhang yan yan ya-lan wu Ge-zhi Xu Wen Ye Ling Wang Guo-xu Xu Guo-tong Xu Wei-ye Li Department of Ophthalmology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China Tongji Eye Institute and Department of Regenerative Medicine Tongji University School of Medicine Shanghai 200092 China Laboratory of Clinical Visual Sciences Institute of Health Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences ＆ Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Ophthalmology Eye and ENT Hospital Fudan University Shanghai 200031 China Department of Ophthalmology Huashan Hospital Affiliated to Fudan University Shanghai 200040 China Department of Ophthalmology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Ophthalmology Second Affiliated Hospital of Soochow University Suzhou Jiangsu 215004 China Department of Ophthalmology Drexel University College of Medicine Philadelphia PA 19107 USA

Objective To investigate the possible involvement of erythropoietin （EPO）/erythropoietin receptor （EPOR） system in neovascularization and vascular regeneration in diabetic retinopathy （DR）. Methods EPOR positive circulating progenitor cells （CPCs： CD34^＋） and endothelial progenitor cells （EPCs： CD34^＋KDR^＋） were assessed by flow cytometry in type 2 diabetic patients with different stages of DR. The cohort consisted of age- and sex-matched control patients without diabetes （n=7）, non-prolif- erative DR （NPDR, n=7）, proliferative DR （PDR, n=8）, and PDR complicated with diabetic nephropathy （PDR-DN, n=7）. Results The numbers of EPOR^＋ CPCs and EPOR^＋ EPCs were reduced remarkably in NPDR corn pared with the control group （both P（0.01）, whereas rebounded in PDR and PDR-DN groups in varying degrees. Similar changes were observed in respect of the proportion of EPOR^＋ CPCs in CPCs （NPDR vs. control, P（0.01） and that of EPOR^＋ EPCs in EPCs （NPDR vs. control, P〈0.05）. Conclusion Exogenous EPO, mediated via the EPO/EPOR system of EPCs, may alleviate the impaired vascular regeneration in NPDR, whereas it might aggravate retinal neovascularization in PDR due to a rebound of EPOR^＋ EPCs associated with ischemia.

关键词： circulating progenitor cells endothelial progenitor cells erythropoietin re-ceptor diabetic retinopathy

Multiple Tin Compounds Modified Carbon Fibers to Construct Heterogeneous Interfaces for Corrosion Prevention and Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 507-527页

作者： Zhiqiang Guo Di lan Zirui Jia Zhenguo Gao Xuetao Shi Mukun He Hua Guo Guanglei wu Pengfei Yin College of Science Sichuan Agricultural UniversityYa’an 625014People’s Republic of China School of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002People’s Republic of China Institute of Materials for Energy and Environment State Key Laboratory of Bio‑Fibers and Eco‑TextilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071People’s Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’anShaanxi 710072People’s Republic of China

Currently,the demand for electromagnetic wave(EMW)absorbing materials with specific functions and capable of withstanding harsh environments is becoming increasingly ***-component interface engineering is considered an effective means to achieve high-efficiency EMW ***,interface modulation engineering has not been fully discussed and has great potential in the field of EMW *** this study,multi-component tin compound fiber composites based on carbon fiber(CF)substrate were prepared by electrospinning,hydrothermal synthesis,and high-temperature thermal *** utilizing the different properties of different substances,rich heterogeneous interfaces are *** effectively promotes charge transfer and enhances interfacial polarization and conduction *** prepared SnS/SnS_(2)/SnO_(2)/CF composites with abundant heterogeneous interfaces have and exhibit excellent EMW absorption properties at a loading of 50 wt%in epoxy *** minimum reflection loss(RL)is−46.74 dB and the maximum effective absorption bandwidth is 5.28 ***,SnS/SnS_(2)/SnO_(2)/CF epoxy composite coatings exhibited long-term corrosion resistance on Q235 steel ***,this study provides an effective strategy for the design of high-efficiency EMW absorbing materials in complex and harsh environments.

关键词： Electrostatic spinning Component regulation Heterogeneous interfaces Electromagnetic wave absorption Corrosion protection

Sine oculis homeobox homolog family function in gastrointestinal cancer:Progression and comprehensive analysis

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World Journal of Clinical Oncology 2025年第1期16卷 10-24页

作者： yang-Zheng lan Zheng wu Wen-Jia Chen Xin-Ning Yu Hua-Tao wu Jing Liu Department of The Breast Center Cancer Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Department of General Surgery First Affiliated Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina

The sine oculis homeobox homolog(SIX)family,a group of transcription factors characterized by a conserved DNA-binding homology domain,plays a critical role in orchestrating embryonic development and organogenesis across various organisms,including *** six distinct members,from SIX1 to SIX6,each member contributes uniquely to the development and differentiation of diverse tissues and organs,underscoring the versatility of the SIX *** or mutations in SIX genes have been implicated in a spectrum of developmental disorders,as well as in tumor initiation and progression,highlighting their pivotal role in maintaining normal developmental trajectories and cellular *** to target the transcriptional complex of the SIX gene family have emerged as a promising strategy to inhibit tumor *** the development of inhibitors targeting this gene family is still in its early stages,the significant potential of such interventions holds promise for future therapeutic ***,this review aimed to comprehensively explore the advancements in understanding the SIX family within gastrointestinal cancers,focusing on its critical role in normal organ development and its implications in gastrointestinal cancers,including gastric,pancreatic,colorectal cancer,and hepatocellular *** conclusion,this review deepened the understanding of the functional roles of the SIX family and explored the potential of utilizing this gene family for the diagnosis,prognosis,and treatment of gastrointestinal cancers.

关键词： Sine oculis homeobox homolog Gastrointestinal cancer Transcription factor Development Regulation Diagnosis Therapeutics

维持性血液透析患者的居家血压变异度及影响因素

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医学研究生学报 2018年第5期31卷 516-520页

作者：李倩倩朱亚梅刘云徐琴娟何亚岚邬步云南京中医药大学护理学院南京护理学硕士研究生210023 江苏省人民医院血液净化中心南京210029 南京中医药大学金陵临床医学院南京210002 苏州大学附属第一医院血液净化中心苏州215006 江苏大学附属医院血液净化中心镇江212000

目的血压变异度(BPV)是维持性血液透析(HD)患者死亡的独立危险因素,目前国内尚无研究HD患者居家BPV影响因素的研究,文中旨在了解HD患者居家BPV状况,探讨影响HD患者BPV的相关影响因素,为临床降低居家BPV提供理论依据。方法选取2017年3-5... 详细信息

目的血压变异度(BPV)是维持性血液透析(HD)患者死亡的独立危险因素,目前国内尚无研究HD患者居家BPV影响因素的研究,文中旨在了解HD患者居家BPV状况,探讨影响HD患者BPV的相关影响因素,为临床降低居家BPV提供理论依据。方法选取2017年3-5月在江苏省人民医院、苏州大学附属第一医院、江苏大学附属医院3所三级甲等医院肾内科透析室行维持性HD的103例患者。收集患者1周7 d家庭监测血压及透析前血压,取家庭监测收缩压的平均值和标准差,以收缩压变异系数表示居家BPV。根据BPV均值分为高BPV组(BPV≥5.8%)和低BPV组(BPV年龄>LVMI。结论在临床护理工作中应积极控制患者透析间期体重增长,鼓励患者进行家庭血压监测,提高患者对居家BPV的重要性认识。同时居家BPV是预测左心室肥厚的重要指标,可作为提高患者自我管理能力的客观工具。

关键词：血液透析血压变异性家庭自测血压影响因素

Double [5]carbohelicene: facile synthesis, chiroptical properties,isomerization study, and lasing application

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Science China Chemistry 2025年第1期 233-240页

作者： Peipei Liu yantong Li Meng-Xiang wu Hao Kang Xiao-Li Zhao Lin Xu Linlin Liu Xiaodong Li Junfeng Fang Zhiwei Fang ya Cheng Hai-Bo yang Huakang Yu Xueliang Shi State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical ProcessesSchool of Chemistry and Molecular Engineering East China Normal University School of Physics and Optoelectronics South China University of Technology Institute of Polymer Optoelectronic Materials & Devices State Key Laboratory of Luminescent Materials & Devices South China University of Technology School of Physics and Electronic Science Engineering Research Center of Nanophotonics & Advanced Instrument Ministry of EducationEast China Normal University State Key Laboratory of Precision Spectroscopy School of Physics and Electronic Science East China Normal University

Double carbohelicenes have attracted considerable attention due to their aesthetic structures, distinct π-conjugation extension, inherent chirality, and intriguing optical and electronic properties. Herein, the concise de novo synthesis of a new double[5]carbohelicene 1 together with its chiroptical properties, isomerization process and lasing application is presented. 1 was synthesized by a simple protocol involving the formation of a 6,6′-bipentacene as the key intermediate. It is worth noting that1 existed as two diastereomers 1-PP/MM and 1-PM, which were successfully isolated and unambiguously confirmed by Xray crystallography. The optical resolution of the racemic 1-PP/MM was successfully achieved by chiral-phase highperformance liquid chromatography（HPLC）, and 1-PP and 1-MM were characterized by circular dichroism. Interestingly, it was found that 1-PP/MM could be completely isomerized to 1-PM upon heating, and the detailed thermodynamics and kinetics of the isomerization process were systematically investigated. 1-PM exhibited deep-red luminescence with emission maximum at 723 nm and fluorescence quantum yield as high as 32.95%. Consequently, we can showcase the lasing application of 1-PM by doping with epoxy resin to assemble a bottle microlaser spontaneously, which the lasing wavelength is 796.6 nm at a threshold of 0.8 m J cm-2. We believe that our studies, including the facile synthesis methodology, detailed isomerization studies, and lasing application, will shed some light on the design and synthesis of novel carbohelical systems with more functions.

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Two new pseudo-isomeric nickel(Ⅱ)metal-organic frameworks with efficient electrocatalytic activity toward methanol oxidation

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Rare Metals 2021年第2期40卷 489-498页

作者： Shan Liu Xiao Wang Hong-Guang Yu ya-Pan wu Bo Li ya-Qian lan Tao wu Jian Zhang Dong-Sheng Li College of Materials and Chemical Engineering Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion MaterialsChina Three Gorges UniversityYichang443002China

Searching for non-noble metal catalysts with high activities and low price is critical to the commercialization of methanol oxidation in the process of fuel ***,two new pseudo-isomeric Ni metal-organic frameworks(Ni-MOFs)(CTGU-17/CTGU-18)have been prepared with 1,4-naphthalenedi-carboxylate(H_(2)NDC),4,4’-dipyridyl amine(DPA)and Ni(ClO_(4))_(2)under hydrothermal *** phase contains one water molecule,but differs dramatically in its bonding to the framework,resulting in different topological networks with fourfold interpenetrating 4-connected dia net(CTGU-17)and(3,7)-connected net(CTGU-18)and diverse electrocatalytic performance for methanol oxidation reaction(MOR).Remarkably,by combining each MOF with the conductive additive,acetylene black(AB),a composite material,AB&CTGU-18(2:4)was confirmed to have an excellent performance for MOR with a high mass specific peak current of 442.3 mA·mg^(-1).The enhanced electrocatalytic activities and robustness might be attributed to the synergetic effect of acetylene black and the active metal centers of MOFs particles for methanol *** present work would provide a new insight for the rational design of MOFs-based composites with enhanced methanol oxidation reaction performance.

关键词： Nickel(Ⅱ) MOFs Composite materials Electrocatalysis Methanol oxidation