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Response Proliferative Capacity of Undifferentiated Stem Cells of Obtained Human Adult Dental Follicle

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Stem Cell Discovery 2014年第4期4卷 125-137页

作者： Larissa Kim higashi de Carvalho Aline Vieira Pinheiro de Araujo Manuela Garcia Laveli da Silva Rosa Andréa Nogueira Laiso Durvanei Augusto Maria Faculty of Medicine University of Sao Paulo Sao Paulo Brazil Laboratory of Biochemistry and Biophysics Butantan Institute Sao Paulo Brazil

Objective: The aim of this study was correlation proliferative activity, markers express stem cells, and lipid peroxides of undifferentiated stem cells of human adult dental follicle (DF) following culture. Methods: For this study, we used 8 samples from DF of impacted third molars to maintain culture conditions and evaluated the growth curve, cell viability, production of lipid peroxidation, cell cycle phases, and proliferative index during 25 days of culture. Results: Cells after culture showed characteristics of fibroblast-like type following 25th day of culture. The results of lipid peroxidation showed that stem cells in culture produce 13 nmoles/ml malondialdehyde at the start of culture, increasing until the 12th day and then began a decline that lasted until the 25th day. We revealed that DFSCs presented a significantly higher percentage of cells in S + G2/M phases by the 15th day of culture compared with cells at the start of culture. Cell surface markers revealed that cell lines were negative for HLA-DR and positive for CD90, CD44, and CD105. The expression of p21 protein, involved in the regulation of the cell cycle, showed a significant increase from the 15th to 25th day of culture. Results of cell division rates show a significant increase between the 6th and 15th day of culture. Conclusions: We conclude that the culture remained stable during the 25 days of culture, presenting the markers of stem cells and markers of control, progression, and cell proliferation that there was an increased production of lipid peroxides between the 6th and 12th days;this increase is related to the increased numbers of cells that also occurs during this period. Then, there is a significantly decline in the production of lipid peroxides and the number of cells, which is accompanied by an increase in cell unviability.

关键词： Follicle Dental Stem Cells Lipid Peroxidation Cell Cycle Proliferation

Females with schizophrenia have abnormal functional cortical connectivity in the gamma frequency during an auditory oddball task using magnetoencephalography

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Open Journal of Psychiatry 2014年第1期4卷 24-38页

作者： Toshiro Fujimoto Eiichi Okumura Kouzou Takeuchi Atsushi Kodabashi Toshiaki Otsubo Katsumi Nakamura Masaki Sekine Shinichiro Kamiya yuji higashi Susumu Shimooki Toshiyo Tamura Faculty of Biomedical Engineering Osaka Electro-Communication University Osaka Japan Fujimoto General Hospital Fujimoto Medical System Miyazaki Japan Neuroscience Project Office Yokogawa Electric Corporation Ishikawa Japan

We studied differences in imaginary coherence (IC) of the gamma band between brain regions of female schizophrenia patients during the auditory oddball task using magnetoencephalography (MEG). Subjects were 12 right-handed female schizophrenia patients, who were evaluated by the Positive and Negative Syndrome Scales (PANSS). Functional connectivity during an auditory oddball task was reconstructed in low gamma (30 - 50 Hz) and high gamma (50 - 100 Hz and 100 - 150 Hz) bands, and represented by IC using seeds determined by the significant oscillatory power changes obtained by event-related synchronization (ERS) and event-related desynchronization (ERD) power measurements. Gamma ERS (30 - 50 Hz) power was decreased in the left precuneus at 500 - 750 ms and in the right precuneus at 750 - 1000 ms. IC in the gamma band (50 - 100 Hz) was decreased between the right precuneus (seed) and right paracentral lobule (target) and between the right precuneus and right hypothalamus at 0 - 250 ms. IC in the gamma band (100 - 150 Hz) was increased between the left precuneus and right cuneus (Brodmann area 7) at 250 - 500 ms, between the left precuneus and right culmen at 500 - 750 ms, and between the left precuneus and right cuneus (Brodmann area 17), between the left precuneus and right posterior cingulate cortex, and between the left precuneus and right caudate nucleus at 750 - 1000 ms. In the high gamma band (50 - 100 Hz) at 0 - 250 ms, significant positive correlations were shown between IC and conceptual disorganization in PANSS scores, between IC and unusual thought content score, and between IC and positive scale score. IC within the high gamma band in female schizophrenia patients showed two types of functional disconnection, intrahemispheric and interhemispheric. IC between the right or left precuneus and other specific cortical areas showed dysfunction, suggesting that the parietal lobe plays an important role in dysfunction in connectivity in the gamma band during the od

关键词： Schizophrenia Female Gamma Band Coherence Functional Connectivity Oddball Magnetoencephalography

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非24小时光周期对生菜生长的影响

农业工程技术 2016年第4期36卷 27-29页

作者： Takanobu higashi Shuhei NISHIKAWA Nobuya OKAMyRA 李明(译) 不详农业部规划设计研究院设施农业研究所

LED是发光二板管(light emitting diode)的简称,具有能耗低、寿命长、体积小、重量轻、发热量少等重要特点,而且LED发出的光线波长范围狭窄,可通过组合提供与植物光合成和光形态建成所需波长一致的光谱,从而满足植物生长需要。很多研究... 详细信息

LED是发光二板管(light emitting diode)的简称,具有能耗低、寿命长、体积小、重量轻、发热量少等重要特点,而且LED发出的光线波长范围狭窄,可通过组合提供与植物光合成和光形态建成所需波长一致的光谱,从而满足植物生长需要。很多研究者针对不同LED光照波长对植物的影响进行了大量研究,为推动LED在农业生产中的应用起到了推动作用。该文则以植物生物钟为切入点,指出可利用LED来调控植物生物钟,从而达到增加产量的目的。该研究对进一步拓宽LED的应用范围,提高LED的利用效率有非常重要的意义。

关键词：植物基因波长范围光合成光形态建成二板增加产量生理节奏文则利用效率农业生产

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氢对Al-8％Mg合金应力腐蚀开裂的影响

轻合金加工技术 1982年第Z3期 94-97页

作者： T.Ohnishni K.higashi Sakai 汪平

铝合金中氢对应力腐蚀开裂的作用,最近以来颇为引人关注。铝合金在应力腐蚀介质中所产生的脆性以及氢脆和应力腐蚀开裂之间的密切关系在许多文献中已有所论述。然而,对氢脆和应力腐蚀开裂之间关系的详细情况并非都非常清楚了。有些作者... 详细信息

铝合金中氢对应力腐蚀开裂的作用,最近以来颇为引人关注。铝合金在应力腐蚀介质中所产生的脆性以及氢脆和应力腐蚀开裂之间的密切关系在许多文献中已有所论述。然而,对氢脆和应力腐蚀开裂之间关系的详细情况并非都非常清楚了。有些作者也研究了Al—8％Mg合金的这些现象,并观察到合金有相当大的脆性,也发现它们两者有着密切的关系。本文的目的在于阐明氢对Al—8％Mg合金应力腐蚀开裂的影响,并以氢脆的观点解释应力腐蚀

关键词：应力腐蚀开裂氢脆应力腐蚀断口 Mg

PERFORMANCE IMPROVEMENT OF AUTONOMOUS FORM MEASURING DEVICE FOR FREE FORM SURFACES

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PERFORMANCE IMPROVEMENT OF AUTONOMOUS FORM MEASURING DEVICE ...

超细纹理超塑性材料Zn-Al合金及其在无铅和地震减振装置方面的应用(英文)

第二届亚洲机械电子学学术会议

作者： Hua QIU Department of Mechanical Engineering,Faculty of Engineering,Kyushu Sangyo University 2-3-1 Matsukadai,higashi-ku,Fukuoka City,813-8503 Japan

This paper reports the author’s recent work to improve the measuring performance of the autonomous form measuring device developed previously,which is applicable to autonomously inspecting profiles of free form surfaces on a machining *** main improvements includes acceptation of a gap sensor to precisely detect the reference position along a canning line,an AC servo motor to smoothly perform the autonomous movement following the profile to be measured,and a new type of laser displacement detector to enhance the measurement capability to the different states of *** results of verification experiments with the new device sufficiently demonstrate the effectiveness of these improvements.

关键词： Autonomous form measurement Free form surface Machining center Performance improvement

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光散射学报 2005年第3期17卷 222-225页

作者： Koichi Makii Atsumichi Kushibe Tsutomu Tanaka Kenji higashi Materials Research. Laboratories Kobe Steel Ltd. 5-5 Takatsukadai 1-chome Nishi-ku Kobe (651-2271) JapanR & Institute Takenaka Corp. 5-1 1-chome Ohtsuka Inzai Chiba (270-1395) JapanDep. of Met. and Mat. Sci. Osaka Pref. University Gakuen-cho 1-1 Sakai(599-8531) Japan Dep. of Met. and Mat. Sci. Osaka Pref. University Gakuen-cho 1-1 Sakai(599-8531) Japan

Since the Great Hanshin Earthquake in 1995, many investigations on the seismic damper have been made. On the other hand, it also theoretically projected that ultra fine grained Zn-Al alloys could show superplasticity at low temperatures [1-4]. Authors tried to make a massive ultra fine grained Zn-22 mass % Al alloy by means of TMCP (thermo-mechanical controlling process). The superplastic Zn-Al alloy is not susceptible to strain deterioration because it has a low work-hardening rate and, thus, does not accumulate strain significantly when deformed plastically. On the other hand, since its work hardening rate is low, plastic deformation proceeds locally, required plastic energy can’t be sufficiently obtained and local fracture and local deformation instability can take place easily. The structural designer must overcome the strain localization resulting from the low work-hardening rate. Several types of seismic dampers were successfully developed with overcoming the local deformation instability. Three types of seismic dampers were finally developed.

关键词：超细纹理超塑性材料 Zn-Al合金地震减振装置机械控制过程

Three-dimensionally Perforated Calcium Phosphate Ceramics

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Journal of Wuhan University of Technology(Materials Science) 2005年第B12期20卷 223-225页

作者： Atsuo Ito T Sakurai y Sogo M Ikeuchi H Ohgushi T Mutsuzaki Institute for Human Science and Biomedical Engineering National Institute of Advanced Industrial Science and Technology Central 6 1-1-1 Higashi Tsukuba 305-8566 Japan Research Institute for Cell Engineering National Institute of Advanced Industrial Science and TechnologyAmagasaki Site 3-11-46 Nakouji Amagasaki Hyogo 661-0974 Japan Department of Orthopedic Surgery University of Tsukuba 1-1-1 Tennodai Tsukuba 305-8571 Japan

Porous calcium phosphate ceramics were produced by compression molding using a special mold followed by sintering. The porous calcium phosphate ceramics have three-dimensional and penetrated open pores 380-400μm in diacneter spaced at intervals of 200μm. The layers of the linear penetration pores alternately lay perpendicular to pore direction. The porosity was 59%-65% . The Ca/ P molar ratios of the porous calcium phos phate ceramics range from 1.5 to 1.85. A binder cantaining methyl cellulose was most effective for preparing the powder compact among vinyl acetate, polyvinyl alcohol, starch, stearic acid, methyl cellulose and their mixtures . Stainless steel, polystyrene, nylon and bamboo were used as the long columnar dies for the penetrated open pores. When polystyrene, nylon and bamboo were used as the long columnar male dies, the dies were burned oat during the sintering process. Using stainless steel as the male dies with the removal of the dies before heat treatment resulted in a higher level of densification of the calcium phosphate ceramic.

关键词： calcium phosphate porous ceramic three-dimensionally perforated tissue engineering scaffold tricalcium phosphate

Clear cell carcinoma of salivary gland type presenting as an endotracheal polypoid tumor

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Case Reports in Clinical Medicine 2013年第2期2卷 159-162页

作者： Kazuhiro Tabata Michiyo higashi Shinichi Kitajima Ikumi Kitazono Tsubasa Hiraki Tsunayuki Otsuka Masaya Aoki Kazuhiro Wakita Hirofumi Nakayama Suguru yonezawa Junya Fukuoka Department of General Thoracic Surgery Kagoshima University Graduate School of Medical and Dental Science Kagoshima Japan Department of Human Pathology Kagoshima University Graduate School of Medical and Dental Sciences Kagoshima Japan Department of Pathology Kagoshima University Hospital Kagoshima Japan Department of Pathology Nagasaki University Graduate School of Biomedical Science Nagasaki Japan Department of Radiology Kagoshima University Graduate School of Medical and Dental Sciences Kagoshima Japan Laboratory of Pathology Nagasaki University Hospital Nagasaki Japan

A 66-year-old woman presented with a 2-year history of asthma has progression of dyspnea within a week, and a computed tomography (CT) revealed an endotracheal tumor at level of aortic arch. The resected tumor, measured by 18 × 12 × 10 mm, has histologically malignant features including frank invasion with desmoplastic stroma and foci of necrosis. Presence of glycogen and mucin was confirmed by periodic acid-Schiff (PAS) and diastase PAS staining. Immunohistochemically, the tumor cells were diffusely positive for cytokeratin (CK) 7 and AE1/3, focally positive for CK5/6 and CK14, but negative for CK20. Thyroid transcription factor-1 (TTF-1), Napsin A, and SP-A, indicating lung parenchymal origin were all negative. Other muscular markers, neuroendocrine markers, renal cell carcinoma markers, and markers related to perivascular epithelioid cell tumors were all negative. Postoperative systemic examination suggests no primary tumor outside the lung. The follow up was excellent being recurrence free for 14 months in spite of the positive surgical margin by the tumor cells. This is a first report ?of rare neoplasm, clear cell carcinoma of salivary gland type, presenting as an endotracheal mass.

关键词： Clear Cell Carcinoma Salivary Gland Lung Primary Histological Feature

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关于和刻本《欧阳文忠公文集》

斯文 2017年第2期 242-254页

作者：东英寿九州大学大学院比较社会文化研究院

由皆川淇园(1734~1805年)和清田儋叟(1719~1785年)编纂的和刻本《欧阳文忠公文集》三十六卷刊行于江户时代宝历十三年(1763)的京都。关于此和刻本的底本,一方面,清水茂氏认为是收录在周必大编纂的《欧阳文忠公集》一百五十三卷中《居士... 详细信息

由皆川淇园(1734~1805年)和清田儋叟(1719~1785年)编纂的和刻本《欧阳文忠公文集》三十六卷刊行于江户时代宝历十三年(1763)的京都。关于此和刻本的底本,一方面,清水茂氏认为是收录在周必大编纂的《欧阳文忠公集》一百五十三卷中《居士集》五十卷的部分,而王岚氏则以为其源于单刻的《居士集》五十卷本系统。然而,从《欧阳文忠公文集》三十六卷的天头,以及各卷末留有周必大编纂《欧阳文忠公集》时所作的校勘来看,清水氏与王岚氏的说法是不能成立的。另一方面,森山秀二氏认为和刻本是以明代曾鲁考异本《居士集》五十卷为基础刻成的。从曾鲁考异本《居士集》五十卷天头部分的校勘与和刻本的校勘大致相同来看,和刻本的校勘确实参考了曾鲁考异本。但是依据皆川淇园《刻欧阳文忠公文集序》的'其家旧蓄欧集两本'的记述来看,其编纂和刻本时共使用了两种欧阳修集版本,除了曾鲁考异本以外的另一种欧阳修集究竟为何种版本,依然不清楚。此外,从和刻本《欧阳文忠公文集》三十六卷的刊行开始,在日本江户时代对以欧阳修为首的唐宋八大家的评价开始提高。可以说,和刻本《欧阳文忠公文集》的刊行,象征着江户时代对于唐宋八大家评价的嬗变。

关键词：欧阳修和刻本《欧阳文忠公文集》曾鲁考异本皆川淇园