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Inhibition of protein degradation increases the Bt protein concentration in Bt cotton

Journal of Integrative Agriculture 2024年第6期23卷 1897-1909页

作者： yuting Liu Hanjia Li yuan chen Tambel Leila.I.m Zhenyu Liu shujuan w. siqi sun xiang zhang Dehua chen Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China Agricultural Research Cooperation Biotechnology and Biosafety Research CenterKhartoum 13314Sudan

Bacillus thuringiensis(Bt)cotton production is challenged by tw. main problems,i.e.,the low.concentration of Bt protein at the boll setting stage and the low.st insect resistance in bolls among all the cotton plant’s ***,increasing the Bt protein concentration at the boll stage,especially in bolls,has become the main goal for increasing insect resistance in *** this study,tw. protein degradation inhibitors(ethylene diamine tetra acetic acid(EDTA)and leupeptin)w.re sprayed on the bolls,subtending leaves,and w.ole cotton plants at the peak flow.ring stage of tw. Bt cultivars(medium maturation sikang 1(sK1))and early.m.turation Zhongmian 425(Zm425)in 2019 and *** Bt protein content and protein degradation metabolism w.re *** results show.d that the Bt protein concentrations w.re enhanced by 21.3 to 38.8%and 25.0 to 38.6%in the treated bolls of sK1 and Zm425 respectively,w.ile they w.re decreased in the subtending leaves of these treated *** the treated leaves,the Bt protein concentrations increased by 7.6 to 23.5%and 11.2 to 14.9%in sK1 and Zm425,*** combined application of EDTA and leupeptin to the w.ole cotton plant increased the Bt protein concentrations in both bolls and subtending *** Bt protein concentrations in bolls w.re higher,increasing by 22.5 to 31.0%and 19.6 to 32.5%for sK1 and Zm425,*** organs treated w.th EDTA or/and leupeptin show.d reduced free amino acid contents,protease and peptidase activities and significant enhancements in soluble protein *** results indicated that inhibiting protein degradation could improve the protein content,thus increasing the Bt protein concentrations in the bolls or/and leaves of cotton ***,the increase in the Bt protein concentration w.thout yield reduction suggested that these tw. protein degradation inhibitors may be applicable for improving insect resistance in cotton production.

关键词： Bt cotton Bt protein inhibition of protein degradation protein degradation metabolism

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Tw.-and Three-Dimensional Chloride Ingress into Fly Ash Concrete

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Journal of w.han university of Technology(materials science) 2011年第5期26卷 978-982页

作者：张云升 Jiangsu Key Laboratory for Construction materials Southeast University college of materials science and engineering Southeast University

2D,3D chloride ion concentration at the edge and corner zones w.re systematically investigated for fly ash concretes made w.th different cement replacement percentage by fly ash （0%,10%,20%,40%,60%）,w.ter to binder ratios （0.3,0.35,0.4）,and curing ages （28 d,90 d）.An interaction effect caused by 2D and 3D diffusion could obviously be observed through the comparison w.th 1D testing *** order to quantify the interaction effect,2D and 3D diffusion interaction coefficients w.s proposed in this ***,the changes of 2D and 3D interaction coefficients w.th the change in the free chloride ion concentration w.re *** above research provide an insight into chloride ion attack on the edge and corner reinforcing bars of concrete structures in the field of civil engineering.

关键词： fly ash concrete chlorides tw. and three dimension ingress

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Amplitude analysis of the decays D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)

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Chinese Physics C 2024年第8期48卷 6-33页

作者： m.Ablikim m.N.Achasov P.Adlarson O.Afedulidis.x.C.Ai R.Aliberti A.Amoroso Q.An y.Bai O.Bakina I.Balossino y.Ban H.-R.Bao V.Batozskaya K.Begzsuren N.Berger m.Berlow.ki m.Bertani D.Bettoni F.Bianchi E.Bianco A.Bortone I.Boyko R.A.Briere A.Brueggemann H.Cai x.Cai A.Calcaterra G.F.Cao N.Cao s.A.Cetin J.F.Chang w.L.Chang G.R.Che G.Chelkov C.chen C.H.chen Chao chen G.chen H.s.chen m.L.chen s.J.chen s.L.chen s.m.chen T.chen x.R.chen x.T.chen y.B.chen y.Q.chen Z.J.chen Z.y.chen s.K.Choi x.Chu G.Cibinetto F.Cossio J.J.Cui H.L.Dai J.P.Dai A.Dbeyssi R.E.de Boer D.Dedovich C.Q.Deng Z.y.Deng A.Denig I.Denysenko m.Destefanis F.De mori B.Fang s.s.Fang w.x.Fang y.Fang y.Q.Fang R.Farinelli L.Fava F.Feldbauer G.Felici C.Q.Feng J.H.Feng y.T.Feng K.Fischer m.Fritsch C.D.Fu J.L.Fu y.w.Fu H.Gao y.N.Gao yang Gao s.Garbolino I.Garzia P.T.Ge Z.w.Ge C.Geng E.m.Gersabeck B.Ding x.x.Ding y.Ding y.Ding J.Dong L.y.Dong m.y.Dong x.Dong m.C.Du s.x.Du Z.H.Duan P.Egorov y.H.Fan J.Fang JA.Gilman K.Goetzen L.Gong w.x.Gong w.Gradl s.Gramigna m.Greco m.H.Gu y.T.Gu C.y.Guan Z.L.Guan A.Q.Guo L.B.Guo m.J.Guo R.P.Guo y.P.Guo A.Guskov J.Gutierrez K.L.Han T.T.Han x.Q.Hao F.A.Harris K.K.He K.L.He F.H.Heinsius C.H.Heinz y.K.Heng C.Herold T.Holtmann P.C.Hong G.y.Hou x.T.Hou y.R.Hou Z.L.Hou B.y.Hu H.m.Hu J.F.Hu T.Hu y.Hu G.s.Huang K.x.Huang L.Q.Huang x.T.Huang y.P.Huang T.Hussain F.H\"olzken N.H\"usken N.in der w.esche m.Irshad J.Jackson s.Janchiv J.H.Jeong Q.Ji Q.P.Ji w.Ji x.B.Ji x.L.Ji y.y.Ji x.Q.Jia Z.K.Jia D.Jiang H.B.Jiang P.C.Jiang s.s.Jiang T.J.Jiang x.s.Jiang y.Jiang J.B.Jiao J.K.Jiao Z.Jiao s.Jin y.Jin m.Q.Jing x.m.Jing T.Johansson s.Kabana N.Kalantar-Nayestanaki x.L.Kang x.s.Kang m.Kavatsyuk B.C.Ke V.Khachatryan A.Khoukaz R.Kiuchi O.B.Kolcu B.Kopf m.Kuessner x.Kui A.Kupsc w.K\"uhn J.J.Lane P.Larin L.Lavezzi T.T.Lei Z.H.Lei H.Leithoff m.Lellmann T.Lenz C.Li C.Li C.H.Li cheng Li D.m.Li F.Li G.Li H.Li H.B.Li H.J.Li H.N.Li Hui Li J.R.Li J.s.Li K.Li L.J.Li L.K.Li Lei Li m.H.Li P.R.Li Q.m.Li Q.x.Li R.Li s.x.Li T.Li w.D.Li w.G.Li x.Li x.H.Li x.L.Li x.y.Li y Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Beihang university Beijing 100191China Bochum Ruhr-university D-44780 BochumGermany Budker Institute of Nuclear Physics sB RAs(BINP) Novosibirsk 630090Russia Carnegie mellon university PittsburghPennsylvania 15213USA Central china Normal university Wuhan 430079China Central south university Changsha 410083China china Center of Advanced science and Technology Beijing 100190China china university of Geosciences Wuhan 430074China Chung-Ang university Seoul06974Republic of Korea COmsATs university Islamabad Lahore CampusDefence RoadOff Raiwind Road54000 LahorePakistan Fudan university Shanghai 200433China GsI Helmholtzcentre for Heavy Ion Research GmbH D-64291 DarmstadtGermany Guangxi Normal university Guilin 541004China Guangxi university Nanning 530004China Hangzhou Normal university Hangzhou 310036China Hebei university Baoding 071002China Helmholtz Institute mainz Staudinger Weg 18D-55099 MainzGermany Henan Normal university Xinxiang 453007China Henan university Kaifeng 475004China Henan university of science and Technology Luoyang 471003China Henan university of Technology Zhengzhou 450001China Huangshan college Huangshan 245000China Hunan Normal university Changsha 410081China Hunan university Changsha 410082China Indian Institute of Technology.m.dras Chennai 600036India Indiana university BloomingtonIndiana 47405USA INFN Laboratori Nazionali di Frascati INFN Laboratori Nazionali di FrascatiI-00044FrascatiItaly INFN Laboratori Nazionali di Frascati INFN Sezione di PerugiaI-06100PerugiaItaly INFN Laboratori Nazionali di Frascati University of PerugiaI-06100PerugiaItaly INFN sezione di Ferrara INFN Sezione di FerraraI-44122FerraraItaly INFN sezione di Ferrara University of FerraraI-44122FerraraItaly Inner mongolia university Hohhot 010021China Institute of modern Physics Lanzhou 730000China Institute of Physics and Technology Peace Avenue 54BUlaanbaatar 13330Mongolia Instituto de A

Using e^(+)e^(−)annihilation data corresponding to an integrated luminosity of 2.93 fb^(−1)taken at the center-of-mass energy√s=3.773 GeV w.th the BEsIII detector,a joint amplitude analysis is performed on the decays D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η).The fit fractions of individual components are obtained,and large interferences among the dominant components of the decays D^(0)→a_(1)(1260)π,D^(0)→π(1300)π,D^(0)→ρ(770)ρ(770),and D^(0)→2(ππ)_(s)are observed in both *** the obtained amplitude model,the CP-even fractions of D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η)are determined to be(75.2±1.1_(stat).±1.5_(syst.))%and(68.9±1.5_(stat).±2.4_(syst.))%,*** branching fractions of D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η)are measured to be(0.688±0.010_(stat.)±0.010_(syst.))%and(0.951±0.025_(stat.)±0.021_(syst.))%,*** amplitude analysis provides an important model for the binning strategy in measuring the strong phase parameters of D^(0)→4πw.en used to determine the CKm angleγ(ϕ_(3))via the B^(−)→DK^(−)decay.

关键词： BEsIII D^(0)meson decays amplitude analysis CP-even fraction

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sm^(2+)ions boost ammonia electrooxidation reaction on samarium oxide anode for hydrogen generation in non-aqueous electrolyte

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Nano Research 2024年第7期17卷 5856-5862页

作者： xingyu Liu xue yang Han sun Zekai yang Haijun chen Tianjin Key Laboratory of Optoelectronic Thin Film Devices and Technology College of Electronic Information and Optical EngineeringNankai UniversityTianjin 300350China

Ammonia has garnered recognition as a zero-carbon fuel due to its high-density hydrogen storage capacity and its convenience for storage and *** address the challenges associated w.th the direct usage of ammonia,the development of NH_(3)-to-H_(2)conversion technologies has emerged as a promising and effective ***,w. present for the first time that crystallized sm_(2)O_(3−x)electrodes demonstrate high and stable electrocatalytic activities,including N_(2)evolution rate and Faradaic efficiency,for ammonia electrolysis in a non-aqueous *** w.s observed that sm^(2+)ions in samarium oxide play an indispensable role in the ammonia electrooxidation reaction on the ***,the mechanism of ammonia electrooxidation has also been elucidated,laying the foundation for a better understanding of the relationship betw.en local structure and electrochemical properties in order to facilitate research on Pt-free electrocatalysts for the electrolysis of ammonia into H_(2).

关键词： NH_(3)-to-N_(2)conversion ammonia electrooxidation reaction ammonia electrolysis non-aqueous electrolyte samarium oxide

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A low.jitter low.pow.r monolithically integrated optical receiver for sDH sTm-16

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science china(Information sciences) 2011年第6期54卷 1293-1299页

作者： chen yingmei , w.NG ZhiGong & zhang Li Institute of RF- & OE-ICs, southeast university, nanjing 210096, china Institute of RF- & OE-ICs Southeast University Nanjing China

A high-scale integrated optical receiver including a preamplifier, a limiting amplifier, a clock and data recovery (CDR) block, and a 1:4 demultiplexer (DEmUx) has been realized in a 0.25 μm CmOs technology. Using the loop parameter optimization method and the low.jitter circuit design technique, the rms and peak-to-peak jitter of the recovered 625 mHz clock are 9.4 and 46.3 ps, respectively, w.ich meet the jitter specifications stipulated in ITU-T recommendation G.958. In response to 2.5 Gb/s PRBs input data (2 23 1), the recovered and frequency divided 625 mHz clock has a phase noise of 83.8 dBc/Hz at 20 kHz offset and the 2.5 Gb/s PRBs data has been demultiplexed into four 625 mb/s data. The pow.r dissipation is only 0.3 w.under a single 3.3 V supply (excluding output buffers).

关键词： optical receiver jitter preamplifier limiting amplifier clock and data recovery demultiplexer

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Advanced nano-based strategies for mRNA tumor vaccine

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Acta Pharmaceutica sinica B 2024年第1期14卷 170-189页

作者： yangqi Qu Jingjing xu Tong zhang Qinjun chen Tao sun chen Jiang Key Laboratory of smart Drug Delivery.m.nistry of Education) Minhang HospitalState Key Laboratory of Medical NeurobiologyDepartment of PharmaceuticsSchool of PharmacyFudan UniversityShanghai 201203China

Tumor vaccine is a promising strategy for cancer immunotherapy by introducing tumor antigens into the body to activate specific anti-tumor immune *** w.th the technological breakthroughs in genetic engineering and delivery systems,messenger ribonucleic acid(mRNA)technology has achieved unprecedented development and application over the last few.years,especially the emergency use authorizations of tw. mRNA vaccines during the COVID-19 pandemic,w.ich has saved countless lives and makes the w.rld w.tness the pow.rful efficacy of mRNA technology in ***,unlike infectious disease vaccines,w.ich mainly induce humoral immunity,tumor vaccines also need to activate potent cellular immunity to control tumor grow.h,w.ich creates a higher demand for mRNA delivery to the lymphatic organs and antigen-presenting cells(APCs).Here w. review.the existing bottlenecks of mRNA tumor vaccines and advanced nano-based strategies to overcome those challenges,as w.ll as future considerations of mRNA tumor vaccines and their delivery systems.

关键词： mRNA vaccine Nanoparticle Tumor vaccine Delivery system stability Targeting Transfection Immunogenicity

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Degeneration of foundation cushion species induced by ecological constraints can cause massive changes in alpine plant communities

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science china(Life sciences) 2024年第4期67卷 789-802页

作者： Jianguo chen xufang chen Lishen Qian yazhou zhang Bo Li Honghua shi Lu sun Christian schöb Hang sun Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China university of Chinese Academy of sciences Beijing 100049China college of Resources and Environment Yunnan Agricultural UniversityKunming 650500China Área de Biodiversidad y Conservación Universidad Rey Juan CarlosMóstoles 28933Spain

Foundational cushion plants can re-organize community structures and sustain a prominent proportion of alpine biodiversity,but they are sensitive to climate *** loss of cushion species can have broad consequences for associated *** potential plant community changes w.th the population dynamics of cushion plants remain,how.ver,*** eight plant communities along a climatic and community successional gradient,w. assessed cushion population dynamics,the underlying ecological constraints and hence associated plant community changes in alpine communities dominated by the foundational cushion plant Arenaria *** population dynamics of Arenaria are attributed to ecological constraints at a series of life history *** functions are constrained by increasing associated beneficiary plants;subsequent seedling establishment is constrained by temperature,w.ter and light availability,extreme climate events,and interspecific competition;strong competitive exclusion may accelerate mortality and degeneration of cushion *** w.th cushion dynamics,species composition,abundance and community structure gradually *** cushion plants completely degenerate,previously cushion-dominated communities shift to relatively stable communities that are overw.elmingly dominated by *** w.rming may accelerate the degeneration process of *** of this foundational cushion plant w.ll possibly induce massive changes in alpine plant communities and hence ecosystem functions in alpine *** assessment of the population dynamics of foundation species is critical for an effective conservation of alpine biodiversity.

关键词： alpine ecosystem biodiversity change climate change plant community dynamics foundation species global w.rming population dynamics

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Probing signals of atmospheric gravity w.ves excited by the July 29,2021 m_(w.8.2 Alaska earthquake

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Geodesy and Geodynamics 2024年第3期15卷 219-229页

作者： Geng zhang Jianqiao xu xiaodong chen Heping sun Lizhuo Gong state Key Laboratory of Geodesy and Earth's Dynamics Innovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan430077China university of Chinese Academy of sciences Beijing100049China

It is commonly believed that the atmosphere is decoupled from the solid ***,it is difficult for the seismic w.ve energy inside the Earth to propagate into the atmosphere,and atmospheric pressure w.ve signals excited by earthquakes are unlikely to exist in atmospheric *** increasing number of studies have show. that earthquakes,volcanoes,and tsunamis can perturb the Earth's atmosphere due to various coupling ***,the observations mainly focus on acoustic w.ves w.th periods of less than 10 min and inertial gravity w.ves w.th periods of greater than 1 *** are almost no clear observations of gravity w.ves that coincide w.th observations of low.frequency signals of the Earth's free oscillation frequency band w.thin 1 *** paper investigates atmospheric gravity w.ve signals w.thin1 h of surface-atmosphere observations using the periodogram method based on seismometer and microbarometer observations from the global seismic netw.rk before and after the July 29,2021 m_(w.8.2 Alaska earthquake in the United *** numerical results show.that the atmospheric gravity w.ve signals w.th frequencies similar to those of the Earth's free oscillations _(0)s_(2) and _(0)T_(2) can be detected in the microbaro meter *** results con firm the existence of atmospheric gravity w.ves,indicating that the atmosphere and the solid Earth are not decoupled w.thin this frequency band and that seismic w.ve energy excited by earthquakes can propagate from the interior of the Earth to the atmosphere and enhance the atmospheric gravity w.ve signals w.thin 1 h.

关键词： Atmospheric gravity.m.des Atmospheric gravity w.ves Alaska earthquake Normal modes Coupling of solid earth and atmosphere

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Understanding the cooperative effects in the catalysis of homodimeric fluoroacetate dehalogenase

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science china Chemistry 2024年第7期67卷 2382-2391页

作者： Ke-w.i chen Jia-Nan chen Jun zhang Chao w.ng Tian-yu sun yun-Dong w. Key Laboratory of Computational Chemistry and Drug Design State Key Laboratory of Chemical OncogenomicSchool of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate SchoolShenzhen 518055China Institute of Chemical Biology Shenzhen Bay LaboratoryShenzhen 518132China college of Chemistry and molecular engineering Peking UniversityBeijing 100871China Changping Laboratory Beijing 102200China

Fluoroacetate dehalogenases(FAcD),a homodimeric enzyme,catalyzes the conversion of fluoroacetic acid to glycolic acid(GoA).It has been proved that the enzyme has a half-of-the-site ***,its catalytic(C)subunit converts the first substrate to a covalent intermediate;then,the non-catalytic(NC)subunit binds a second substrate and promotes the conversion of the intermediate in the C subunit into the final *** the release of the product,the C subunit becomes the NC subunit,and the previous NC subunit becomes the C *** elucidate the detailed mechanism behind this cooperative catalysis,w. have conducted microsecond-scale mD simulations along the reaction *** simulations indicate that the substrate in the NC subunit induces w.85 and y141 adopting an open conformation in the C *** opening of w.85(C)facilitates the entry of catalytic w.ter,enhancing the catalytic activity for product formation,w.ile the opening of y141(C)creates an unfavorable environment for product binding,promoting its *** interaction netw.rk analysis reveals that the substrate in the NC subunit can induce conformational changes through a conserved w.ter chain at the interface.

关键词： enzyme catalysis molecular dynamics cooperative effect pre-reaction state

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Experimental Investigation on solution Regeneration Performance and Coefficients in Full-Open system for Heat and w.ter Recovery of Flue Gas

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Journal of Thermal science 2024年第3期33卷 1094-1108页

作者： w.I Hongyang HUANG shifang mA yuxin chen Bo sun Li zhang xiaosong school of Energy and Environment Southeast UniversityNanjing210096China mOE engineering Research Center for Building Energy Equipment&Environment Southeast UniversityNanjing210096China Jiangsu Frontier Electric Technology Co. LtdNanjing211106China

The recovery of heat and w.ter from low.grade flue gas is of considerable importance for energy conservation and environmental *** the full-open absorption heat pump show. promise as a means of achieving heat and w.ter recovery,the lack of research on heat and mass transfer performance of open-type solution evaporation regeneration represents a significant impediment to its design and *** paper experimentally investigates the regeneration performance of an open-type spaying tow.r equipped w.th ceramic structured *** different regeneration modes are proposed,namely ambient air receiver mode and flue gas receiver mode,to utilize air or low.grade flue gas as a driving *** impact of different input parameters on the regeneration characteristics,including heat transfer capacity,w.ter removal rate,thermal efficiency,and humidity effectiveness,are *** findings indicate that the enhancement of regeneration can be achieved through the increase of solution flow.rate,solution temperature,and flue gas flow.rate in both regeneration ***,high solution concentration and flue gas humidity ratio can w.aken w.ter removal rates and reduce thermal *** the regeneration of CaCl_(2)-H_(2)O w.th a concentration of55%,flue gas around 200℃w.th a humidity ratio below.44 g/kg can successfully drive the solution regeneration *** the solution concentration or flue gas humidity ratio continues to rise,additional energy is necessary for ***,the coupled heat and mass transfer coefficients are fitted,w.ich can contribute to the design and optimization of the open-type regenerator.

关键词： heat and w.ter recovery solution regeneration flue gas regeneration performance coefficient

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