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An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

Horticultural Plant Journal 2024年第4期10卷 983-994页

作者： Xiaoxiao yu Jintai yu yin Lu Wenjing Li Guanzhong Huo Jun Zhang yan Li Jianjun Zhao Jun Li State Key Laboratory of North China Crop Improvement and Regulation College of Life SciencesHebei Agricultural UniversityBaodingHebei 071001China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaodingHebei 071001China Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei Collaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071001China College of Modern Science and Technology Hebei Agricultural UniversityBaodingHebei 071001China Human Resource Department Hebei Agricultural UniversityBaodingHebei 071001China

Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less *** order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene *** established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×1.^(5)·g^(-1.FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection ***,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and ***,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.

关键词： Chinese cabbage PTGE Transfection efficiency CRISPR/Cas9

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A robust viscoelastic surfactant tolerating 20% HCl up to 1.0℃ for oil well stimulation

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Petroleum Science 2024年第3期21卷 1865-1874页

作者： Ji Wang Ning Qi Hong-yao yin yu-Jun Feng Polymer Research Institute State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu610065SichuanPR.China Tianfu yongxing Laboratory New Theory and Technology of CO_(2) Capture Research CenterChengdu610065SichuanPR China School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongPR China

Viscoelastic surfactants(VES)are often used as viscous diverters in acidizing stimulation to prolong the acid consumption time and maximize zonal coverage of the acid for improving well ***,the ceiling temperature of commercial VES cannot exceed 1.0℃in practical use because of the poor thermal stability and fragile molecular structure,hindering their implementation in hightemperature oil reservoirs,i.e.,≥1.0℃.Here we synthesized a novel C22-tailed diamine,N-erucaminopropyl-N,N-dimethylamine(EDPA),and examined comparatively its rheological behavior,assemblies morphology and molecular stability in 20 wt%HCl with a commercial VES,erucyl dimethyl amidopropyl betaine(EDAB).The feasibility of EDPA for acidizing stimulation was assessed by acid etching of carbonate rock with its HCl solution at 1.0℃.Rheological results showed that the 2.5 wt%EDPA—20 wt%HCl solution maintains stable viscosity of 90 m Pa s at 1.0℃for 60 min,while that of 2.0 wt%EDAB HCl solution is just 1.m Pa s under identical conditions.1. NMR spectra and cryo-TEM observations revealed that the chemical structure and self-assembled architectures of EDPA remained intact in such context,but the EDAB suffered from degradation due to the hydrolysis of the amide group,accounting for the poor heat-resistance and *** reaction rate of 2.5 wt%EDPA HCl solution with carbonate rock was one order of magnitude lower than that of 20 wt%HCl solution at 1.0℃,underpinning the potential of EDPA to be used in the high-temperature reservoirs *** work improved the thermal tolerance of VES in highly concentrated HCl solution,paving a feasible way for the acidization of high-temperature reservoir environments(~1.0℃).

关键词： Viscoelastic surfactants Thermal stability Self-diverting acid Wormlike micelles Acidizing stimulation

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Force-induced Caspase-1.dependent pyroptosis regulates orthodontic tooth movement

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International Journal of Oral Science 2024年第2期16卷 238-250页

作者： Liyuan Chen Huajie yu Zixin Li yu Wang Shanshan Jin Min yu Lisha Zhu Chengye Ding Xiaolan Wu Tianhao Wu Chunlei Xun yanheng Zhou Danqing He yan Liu Department of Orthodontics Central LaboratoryPeking University School and Hospital for Stomatology&National Center for Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Research Center of Oral Biomaterials and Digital Medical Devices&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental Materials&National Engineering Research Center of Oral Biomaterials and Digital Medical DevicesBeijingChina Peking University Hospital of Stomatology Fourth Division BeijingChina

Pyroptosis,an inflammatory caspase-dependent programmed cell death,plays a vital role in maintaining tissue homeostasis and activating inflammatory *** tooth movement(OTM)is an aseptic force-induced inflammatory bone remodeling process mediated by the activation of periodontal ligament(PDL)progenitor ***,whether and how force induces PDL progenitor cell pyroptosis,thereby influencing OTM and alveolar bone remodeling remains *** this study,we found that mechanical force induced the expression of pyroptosis-related markers in rat OTM and alveolar bone remodeling *** or enhancing pyroptosis level could suppress or promote OTM and alveolar bone remodeling *** Caspase-1.(−/−)mice,we further demonstrated that the functional role of the force-induced pyroptosis in PDL progenitor cells depended on ***,mechanical force could also induce pyroptosis in human ex-vivo force-treated PDL progenitor cells and in compressive force-loaded PDL progenitor cells in vitro,which influenced ***,transient receptor potential subfamily V member 4 signaling was involved in force-induced Caspase-1.dependent pyroptosis in PDL progenitor ***,this study suggested a novel mechanism contributing to the modulation of osteoclastogenesis and alveolar bone remodeling under mechanical stimuli,indicating a promising approach to accelerate OTM by targeting Caspase-1.

关键词： homeostasis movement thereby

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Highly efficient photo-thermal synergistic catalysis of CO_(2)methanation over La_(1.x)Ce_(x)NiO_(3)perovskite-catalyst

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Nano Research 2024年第9期17卷 7945-7956页

作者： Ting Li Zhen-yu Zhang De-Cun Luo Bo-yu Xu Rong-Jiang Zhang Ji-Long yao Dan Li Tao Xie Institute of Industrial Catalysis School of Chemical Engineering and TechnologyXi’an Jiaotong UniversityXi’an 710049China

Solar-driven photo-thermal catalytic CO_(2)methanation reaction is a promising technology to alleviate the problems posed by greenhouse gases ***,designing advanced photo-thermal catalysts remains a research challenge for CO_(2)methanation *** this work,a series of ABO3(A=lanthanide,B=transition metal)perovskite catalysts with Ce-substituted LaNiO3(La_(1.x)Ce_(x)NiO_(3),x=0,0.2,0.5,0.8,1.were synthesized for CO_(2)*** La_(0.2)Ce_(0.8)NiO_(3) exhibited the highest CH_(4) formation rate of 258.9 mmol·g^(-1.·hcat-1.CO_(2)conversion of 55.4%and 97.2%CH_(4) selectivity at 300℃with the light intensity of 2.9 W·cm^(-2).Then the catalysts were thoroughly analyzed by physicochemical structure and optical properties *** partial substitution of the A-site provided more active sites for the adsorption and activation of CO_(2)/H_(2).The sources of the active sites were considered to be the oxygen vacancies(O_(v))created by lattice distortions due to different species of ions(La^(3+),Ce^(4+),Ce^(3+))and exsolved Ni0 by H_(2)*** catalysts have excellent light absorption absorbance and low electron-hole(e^(-)/h^(+))recombination rate,which greatly contribute to the excellent performance in photo-thermal synergistic catalysis(PTC)CO_(2)*** results of in situ irradiated electron paramagnetic resonance spectrometer(ISI-EPR)and ISI-X-ray photoelectron spectroscopy(XPS)indicated that the aggregation of unpaired electrons near the defects and Ni metal(from La and Ce ions to Ov and Ni0)accelerated adsorption and activation of CO_(2)/H_(2).At last,the catalyst properties and structure were correlated with the proposed reaction mechanism from the in situ diffuse reflection infrared Fourier transform spectrum(DRIFTS)*** in situ precipitation of the B-site enhanced the dispersion of Ni,while its enriched photoelectrons upon illumination further promote hydrogen *** H^(*)spillover accelerated t

关键词： photo-thermal catalysis CO_(2)methanation perovskite A-site substitution in situ diffuse reflection infrared Fourier transform spectrum(DRIFTS)

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Sequence-Controlled Synthesis of Alternating Poly(ester amide)s using Water as Control Agent in One-Pot

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Chinese Journal of Polymer Science 2024年第8期42卷 1015-1020页

作者： Tao Liu Fang-Fang Niu Kan Cheng yu-Min Xia Bo-Mou Ma Xue-Li Wang Jian-yong yu yong He State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China Innovation Center for Textile Science and Technology Donghua UniversityShanghai 201620China

Poly(ester amide)s(PEAs)attract much attention as a new kind of biodegradable ***,the synthesis of PEAs with sequence-regulated chain structures is still complicated due to the multistep polymerization,high monomer purity,as well as the usage of organic solvents,which greatly inhibits its development and ***,a one-pot strategy without solvent was developed to synthesize the alternating PEA fromα,ω-amino alcohol and dicarboxylic acid by sequential polycondensation,where water was used as the controlling agent for amidation and ***,the amidation and esterification were controlled to proceed in turn by adding or removing water,realizing the one-pot preparation of alternating *** resultant PEA is characterized by^1.-and^(1.)C-NMR,and its chain structure is confirmed to be highly quasi-alternating similar to alternating PEAs prepared by classical methods The evaluations from DSC and DMA demonstrate that the properties of quasi-alternating PEA(T_(m)=1.2℃,ΔH_(m)=30J·g^(-1.)are far superior to those of random PEA(T_(m)=72℃,ΔH_(m)1.J·g^(-1.).

关键词： Biodegradable macromolecules Polymer architectures Sequence-controlled synthesis One-pot polymerization

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Off-design condition optimization of organic Rankine cycle based on genetic algorithm

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Energy and Built Environment 2024年第5期5卷 665-682页

作者： Shiqi Wang Zhongyuan yuan Nanyang yu School of Mechanical Engineering Southwest Jiaotong UniversityNo.111North Section 12nd Ring RoadChengdu610031China

Organic Rankine cycle(ORC)has been considered as one of the most promising technologies in industrial waste heat utilization and power *** the actual operation of ORC system,due to the fluctuation of cooling and heat sources,the system operates under off-design conditions in most *** this paper,thermodynamic model,heat transfer process description and power equipment model are established to evaluate the operating parameters of ORC for the off-design *** temperature and condensation temperature are taken as independent parameters for the operation of ORC *** algorithm is adopted to optimize the independent parameters under the maximum net output *** results show that the effect of optimizing independent parameters is to make the working fluid at the outlet of the preheater as close as possible to a saturated liquid state,and the working fluid at the inlet of the screw expander should be in a saturated gas *** the optimal power output increasing by 1..1.for every 5°C increase in hot water inlet temperature,9.2%for every 20 kg/s increase in hot water mass flow rate,and 3.9%for every 1.C decrease in cooling water *** optimization method of off-design operating conditions has good system performance and good engineering application prospects.

关键词： Organic rankine cycle Optimize operation Genetic algorithm Net output power

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Unlocking stability:Preserving activity of biomimetic catalysts with covalent organic framework cladding

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Chinese Chemical Letters 2024年第6期35卷 1-2页

作者： Ting Wang Xin yu yaqiang Xie College of the Environment&Ecology Xiamen UniversityXiamen 361102China

Enzymes are extremely complicated biocatalysts in several industrial processes due to their numerous advantages over conventional catalysts,including lower physiological and environmental toxicity,exceptional selectivity,and milder reaction conditions[1..However,their widespread applications are constrained by the high production costs,low operational stability,and complexity involved in the recovery and reusability of enzymes[2].

关键词： stability catalysts exceptional

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Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1

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Plant Communications 2024年第5期5卷 9-24页

作者： yang yu Haixia yu Jing Peng Wang Jinsong yao yi Peng Wang Feng Li Zhang Shi Rong Wang yajie Zhao Xiang yu Zhao Xian Sheng Zhang ying Hua Su State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’anShandong 271018China

In the realm of genetically transformed crops,the process of plant regeneration holds utmost ***,the low regeneration efficiency of several wheat varieties currently restricts the use of genetic transformation for gene functional analysis and improved crop *** research explores overex-pression of TaLAX PANICLE1.TaLAX1.,which markedly enhances regeneration efficiency,thereby boost-ing genetic transformation and genome editing in *** noteworthy is the substantial increase in regeneration efficiency of common wheat varieties previously regarded as recalcitrant to genetic *** study shows that increased expression of TaGROWTH-REGULATING FACTOR(TaGRF)genes,alongside that of their co-factor,TaGRF-INTERACTING FACTOR 1.TaGIF1.,enhances cytokinin accumulation and auxin response,which may play pivotal roles in the improved regeneration and transfor-mation of TaLAX1.overexpressing wheat *** of TaLAX1.homologs also significantly in-creases the regeneration efficiency of maize and soybean,suggesting that both monocot and dicot crops can benefit from this *** shed light on a gene that enhances wheat genetic trans-formation and elucidate molecular mechanisms that potentially underlie wheat regeneration.

关键词： plant regeneration genetic transformation TaLAX1 TaGRF4–TaGIF1 cytokinin auxin wheat

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Epitranscriptomic Regulation of NMDA Receptors Rears its Ugly Head in Chemotherapy-Induced Neuropathic Pain

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Neuroscience Bulletin 2024年第8期40卷 1209-1211页

作者： Wing Lam yu Gerald W.Zamponi Department of Clinical Neurosciences Hotchkiss Brain InstituteAlberta Children's Hospital Research InstituteUniversity of CalgaryCalgaryAB T2N 4N1Canada

A number of different cancer chemotherapy agents such as cisplatin,oxaliplatin,and paclitaxel can lead to nerve damage,thereby giving rise to neuropathic pain states that present with mechanical and cold allodynia[1..Although these pain conditions often resolve after completion of the chemotherapy,a significant portion of patients exhibit longlasting pain that can persist longer than 1. months with a negative impact on the quality of life of affected individuals[1..

关键词： chemotherapy pain damage

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Study on tungsten sintering temperature field based on electromagnetic simulation and computational fluid dynamics

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Tungsten 2024年第2期6卷 355-366页

作者： Lu-yan Wang yue-Guang yu Jian-Xin Dong Kai-Ping Du Shan-yu Liu Cao-Bing Li University of Science and Technology Beijing Beijing 100083China BGRIMM Technology Group Beijing 100160China China Iron&Steel Research Institute Group Beijing 100081China

In order to study the influence of various sintering variables more intuitively and clearly,electromagnetic field analysis and computational fl uid dynamics are used to analyze the infl uence of gas flow,heating power and other process parameters on the temperature range of medium-frequency sintering in this *** results indicate that the uniformity of temperature fi eld is determined by the gas flow rate and heating *** the heating power is 50 kW,the stable temperature drops about 1. K for every 1.m^(3)·h^(−1.increase of hydrogen *** peak value of the maximum temperature diff erence on the tungsten rods gradually increases with the increase of the hydrogen flow rate,and it appears slightly *** temperature distribution of tungsten rods in furnace is similar with diff erent hydrogen flow *** the same time,the closer to the furnace wall,the higher the temperature of the tungsten *** temperature diff erence between tungsten rods mainly occurred within 1.h after the beginning of *** the heating power can improve the stable temperature,but has little eff ect on the heating *** heating power can increase the peak value of the maximum temperature diff erence on the tungsten rods,but has little eff ect on the occurrence time of the peak value of the maximum temperature diff erence on the tungsten *** the heating power will increase the temperature diff erence of a single tungsten rod,but the temperature diff erence of a single tungsten rod never exceeds 65 *** study can be used to guide the process optimization *** with numerical simulation calculation,the sintering parameters with low energy consumption and high temperature uniformity can be quickly and eff ectively found,thus improving the microstructure and properties of sintered tungsten products.

关键词： Tungsten products Sintering Hydrogen flow rate Heating power

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